- Open Access
Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001
SpringerPlus volume 2, Article number: 154 (2013)
Amylases are one of the most important enzymes in present-day biotechnology. The present study was concerned with the production and partial characterization of extracellular amylase from Bacillus amyloliquefaciens P-001. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Enzyme production was induced by a variety of starchy substrate but corn flour was found to be a suitable natural source for maximum production. Tryptone and ammonium nitrate (0.2%) as nitrogen sources gave higher yield compared to other nitrogen sources. Maximum enzyme production was obtained after 48 hrs of incubation in a fermentation medium with initial pH 9.0 at 42°C under continuous agitation at 150 rpm. The size of inoculum was also optimized which was found to be 1% (v/v). Enzyme production was 2.43 times higher after optimizing the production conditions as compared to the basal media. Studies on crude amylase revealed that optimum pH, temperature and reaction time of enzyme activity was 6.5, 60°C and 40 minutes respectively. About 73% of the activity retained after heating the crude enzyme solution at 50°C for 30 min. The enzyme was activated by Ca2+ (relative activity 146.25%). It was strongly inhibited by Mn2+, Zn2+ and Cu2+, but less affected by Mg2+ and Fe2+.
Microbial enzymes are widely used in industrial processes due to their low cost, large productivity, chemical stability, environmental protection, plasticity and vast availability (Burhan et al.2003; Mishra &Behera 2008). Bacillus species such as Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus licheniformis are used as bacterial workhorses in industrial microbial cultivations for the production of a variety of enzymes as well as fine biochemicals for decades. A large quantity (20-25g/l) of extracellular enzymes has been produced and secreted by the various Bacillus strains which have placed them among the most significant industrial enzyme producers. The estimated value of world market is presently about US$ 2.7 billion and is estimated to increase by 4% annually through 2012. Detergents (37%), textiles (12%), starch (11%), baking (8%) and animal feed (6%) are the main industries, which use about 75% of industrially produced enzymes (Harwood 1992; Ferrari et al.1993; Schallmey et al.2004; Das et al.2011). Amylases are among the most important enzymes and are of great significance for biotechnology, constituting a class of industrial enzymes having approximately 25-30% of the world enzyme market (Azad et al.2009; Rajagopalan &Krishnan 2008). Initially the term amylase was used originally to designate an extracellular enzymes capable of hydrolyzing α-1,4-glucosidic linkages in polysaccharides containing three or more 1,4-α-linked glucose units. The enzyme acts on starches, glycogen and oligosaccharides in a random manner, liberating reducing groups. These enzymes are found in prokaryote as well as in eukaryotic organisms. They are widely distributed in microbial, plant and animal kingdoms. In the present day scenario, amylases have a great commercial value in biotechnological applications ranging from food, fermentation, textile to paper industries. These uses have placed greater stress on increasing amylase production and search for more efficient processes (Aehle &Misset 1999; Lin &Hsu 1997;Wolfgang 2007). For the maximum enzyme production, medium optimization is a prime step for its commercial usage.
The present work describes the effects of culture conditions on amylase production in batch experiments in shake flasks and under controlled conditions in a laboratory incubator. In this study, we show that enzyme synthesis is affected by carbon and nitrogen sources and maximal activity is attained with inorganic than organic nitrogen sources. The optimum enzyme production by the bacterial isolate was found at 42°C, whereas maximum enzyme activity was observed at 60°C. The enzyme was activated by Ca2+ (relative activity 146.25%). It was strongly inhibited by Mn2+, Zn2+ and Cu2+, but less affected by Mg2+ and Fe2+.
Results and Discussion
The genus Bacillus produces a large variety of extracellular enzymes, of which amylases are of particularly considerable industrial importance (Swain et al.2006). Present study deals with the production condition optimization and partial characterization of crude extracellular amylase produced by Bacillus amyloliquefaciens P-001. Bacillus amyloliquefaciens P-001 were able to hydrolyze starch showing zone of hydrolysis around the colonies on agar medium supplemented with soluble starch (Figure 1).
Therefore, further studies on enzyme production in shake-flask cultures were carried out using Bacillus amyloliquefaciens P-001. The organism was used for extracellular amylase production in shake-flask culture using basal medium (0.1% KH2PO4, 0.25% Na2 HPO4, 0.1% NaCl, 0.2% (NH4)2SO4, 0.005% MgSO4 .7H2O, 0.005% CaCl2, 0.2% tryptone and 1% soluble starch, pH 6.5) (Bose &Das 1996) for 48 hrs hours of incubation at 37°C and enzyme activity was obtained 35.0 U/ml. To enhance the production of enzyme various parameters associated with the production of amylase were studied in the medium used for the enzyme production. Optimization of culture conditions is very important for maximum microbial growth and enzyme production by microorganisms (Kathiresan &Manivannan 2006). Among the physical and chemical parameters, optimum temperature, pH range, carbon and nitrogen sources are the most important for enzyme production by microbes (Bose &Das 1996; Gupta et al.2003).
Among physical parameters, pH of the growth medium plays an important role in enzyme secretion. The pH range observed during the growth of microbes also affects product stability in the medium (Banargee &Bhattacharya 1992). Most of the earlier studies revealed an optimum pH range between 6.0 and 7.0 for the growth of bacterial strains and enzyme production (Gupta et al.2003; Kundu et al.1973; Castro et al.1992). Previous studies have revealed that fungi required slightly acidic pH and bacteria required neutral pH for optimum growth (Gangadharan et al.2006). So, the effect of initial pH on the production of amylase by Bacillus amyloliquefaciens P-001 was investigated at different pH (6.0-9.5). The activity of the enzyme was obtained at slightly alkaline pH 9.0. But, the final pH of the medium (initial pH 9.0) of shake flask fermentation was 7.5. At neutral pH, the results were moderate and at acidic pH the enzyme activity was extremely low (Table 1). It might be due to the fact that the enzyme was inactive in the acidic medium (Castro et al.1993). (Nusrat &Rahman 2007) reported that, α-amylase production at pH 7.0 by the Bacillus amyloliquefaciens was maximum. Another study conducted by O. El-Tayeb (2007) showed that alpha amylase production by Bacillus amyloliquefaciens (strain 267CH) in fermentor was highest at pH 6.0. (El-Tayeb et al.2007).
Temperature is a vital environmental factor which controls the growth and production of metabolites by microorganisms and this is usually varied from one organism to another (Banargee &Bhattacharya 1992; Kumar &Takagi 1999). Bacterial amylases are produced at a much wider range of temperature. Bacillus amyloliquefaciens, B.subtilis, B. licheniformis and B. stearothermophilus are among the most commonly used Bacillus sp. reported to produce α-amylase at temperatures 37–60°C (Mendu et al.2005;Mielenz 1983; Syu &Chen 1997; Mishra et al.2005). A wide range of temperature (35-80°C) has been reported for optimum growth and α-amylase production in bacteria (Burhan et al.2003; Castro et al.1992; Prakash et al.2009; Lin et al.1998). In present study, for the determination of optimum temperature for enzyme production, the fermentation was carried out at different temperatures (32 to 45°C). Enzyme production was gradually increased with increasing temperature and maximum enzyme production was observed at 42°C (Table 1). The optimum range for enzyme production was 40-42°C. Nusrat &Rahman (2007) reported that, α-amylase production was maximum at temperature 37°C by the Bacillus amyloliquefaciens. Haq et al. (2010) reported that, the better activity of α-amylase in stirred fermentor with working volume of 4.5 L was at 37°C in 48 h by using randomly induced mutant strain of Bacillus amyloliquefaciens EMS 6.
From the time course study in shake culture, it was found that the rate of enzyme production was increased with the increase in the fermentation period and reached its maximum activity after 48 hour incubation (Table 1). The total protein content obtained was 3.97 mg/ml at that time. A prolonged incubation time beyond 48 hour did not increase the enzyme production. These findings are similar to the result reported by Haq et al. (2010). A similar result was also found by Asgher et al. (2007) studied on Bacillus subtilis, Kaur &Vyas (2012) in case of Bacillus sp. DLB 9 and Riaz (et al.2003) in case of Bacillus subtilis. It might be due to the accumulation of other by products in the medium Riaz et al. (2003). Efficient induction might not occur until the stationary phase has been reached and the available carbon source was reduced (Huang et al.2003; Wanderley et al.2004). But, Abate et al. (1999) reported that the production of α-amylase by Bacillus amyloliquefaciens starts at the beginning of the exponential growth phase reaching the maximum level after 24 hour and after that, α-amylase level decreased drastically probably due to the accumulation of high level of protease activities concomitant with the sporulation process at the end of the exponential growth phase. Similar findings have been reported on Bacillus amyloliquefaciens Hillier et al. (1997) and Bacillus sp. ANT-6 Burhan et al. (2003).
The volume of inoculum plays an important role in the fermentation of enzymes Lin et al. (1998). In our study, 1% inoculum induced the maximum amylase production (Table 1). As the inoculum level was further increased, the production of enzyme was gradually decreased. It may be due to the fact that at high concentration of inoculum level, the bacteria grow rapidly and the nutrients present in the media were insufficient to overcome the growth of bacteria. Thus, the production of amylase was affected at higher concentration of inoculum. Our findings are in a good agreement with Riaz et al. (2003).
Natural sources could serve as economical and readily available raw material for the production of valuable enzymes. Agricultural wastes are being used for liquid fermentation to reduce the cost of fermentation media. These wastes consist of different carbon sources are necessary for the growth of microorganisms (Haq et al.2005; Swamy &Seenayya 1996; Djekrif-Dakhmouche et al.2005). The nature and amount of carbon sources in culture media are important factor for the production of extracellular amylaseAkcan (2011). It was found that α-amylase production was maximum when starch was used as the carbon source (Gupta et al.2003; Sumrin et al.2011; Bandyopadhyay et al.1993; Lin et al.1994; Narang &Satyanarayana 2001; Bozic et al.2011; Sexana et al.2007). Biosynthesis of the enzyme took place not only in the presence of starch but also with other carbon sources. Our study showed that the production of enzyme was highest when corn flour was used as carbon source in the basal media (Figure 2). Besides, rice flour and wheat bran showed moderate effects on enzyme synthesis. Earlier studies reported that, complex substrates induce higher amylase production Sexana et al. (2007).
The nature and relative concentration of different complex nitrogenous sources in the growth medium are both important in the synthesis of amylase. Lower levels of nitrogen and also excess nitrogen are equally detrimental causing enzyme inhibition Sharma et al. (2012). The influence of organic nitrogen sources on amylase production was determined. Among the different organic nitrogen sources tested, tryptone (0.2%) was found to be a good nitrogen source for amylase production from Bacillus amyloliquefaciens P-001 (Figure 3). In fact, tryptone has been reported to be the best nitrogen source for amylase production Okalo et al. (1996). In present study, yeast extract, casein and beef extract also showed stimulating effects on amylase synthesis. It has been reported that yeast extract also served as good organic nitrogen source for α-amylase synthesis from Bacillus amyloliquefaciens (Sharma et al.2012; Magee &Kosaric 1987). Similarly, casein was reported to be a good nitrogen source for α-amylase production from B. subtilis IP 5832 Bozic et al. (2011). It was observed that inorganic nitrogen sources gave comparatively higher yields than organic nitrogen sources. In present study, the enzyme production was increased when ammonium nitrate used as inorganic nitrogen source in the culture media. According to Coleman &Elliott (1962) ammonium salts were stimulators of B. subtilis amylase synthesis. Our findings are in a good agreement with the findings of these studies (Figure 4). It has also been reported that, ammonium nitrate and sodium nitrate were the best nitrogen sources for maximum amylase production (Kundu et al.1973; Mahmood &Rahman 2008). Ammonium chloride, ammonium sulphate showed stimulating effects on amylase production. It has been found that α-amylase production by B. subtilis DM-03 was maximum when ammonium chloride as the nitrogen source Das et al. (2004). Swain et al. (2006) studied on Bacillus subtilis reporting that, urea inhibited α-amylase activity which is similar to our findings.
A pH range from 5.5-8.0 was used to study the effect of pH on amylase activity (Figure 5) and optimum pH was found at 6.5. In the alkaline pH range, the activity was lower. The effect of temperature on enzyme activity was assayed at different temperatures ranging from 35-70°C at optimum pH. The results showed that enzyme activity was increased with temperature and it showed highest activity at temperature 60°C (Figure 6). Above 60°C temperature activity was also decreased. For determination of optimum reaction time, enzyme assay was carried out at different reaction time ranging from 5–50 minutes at constant temperature and pH. It was observed (Figure 7) that, the enzyme exhibited its maximum activity at 40 min of reaction time. Aygan et al. (2008) in 2008 reported that enzyme obtained from Bacillus sp. AB68 was active in a broad temperature range between 20 and 90°C, with an optimum of 50°C. Stability of the enzyme is of great importance for the economy of their industrial application. In case of thermostabilty, the enzyme was pre incubated at different temperatures for 30 min and then enzyme was assayed. The results (Figure 8) showed that the enzyme activity was retained 73% after heating at 50°C for 30 min. After this time the activity was decreased drastically and enzyme was completely inactivated when heated at 80°C. Thus, the results concluded that the crude enzyme is moderately temperature stable. It is therefore worthwhile to consider means to stabilize the enzyme under storage conditions. Temperature is an important limiting factor for storage of enzymes. In our study, enzyme was stored at room temperature for 21 days. But, the room temperature was moderately suitable for the storage of this enzyme in considering commercialization and industrial application, thus causing the rapid reduction of enzyme activity. Only 66% of the activity retained at room temperature after 21 days (Figure 9).
Most of amylases are known to be metal ion-dependent enzymes, namely divalent ions like Ca2+, Mg2+, Mn2+, Zn2+, Fe2+ etc. Pandey et al. (2000). The effect of metal ions on amylase activity was measured in the presence of various metal ions at a concentration of 2 mM. Activities of enzyme were stimulated in the presence of Ca2+, Mg2+ and Fe2+ ions (Figure 10). On the other hand, a strong inhibitory effect was observed in the presence of Cu2+, Zn2+ and Mn2+. Results suggest that amylase did not require any metal ions for catalytic activity except Ca2+ and was activated (relative activity 146%) by calcium.Tonkova (1991) and Chung et al. (1995) stated that, addition of Ca2+ ion is often significant for production and stability of amylase of many Bacillus spp. According to Gupta et al. (2003), α-amylase contain at least one Ca2+ ion and affinity of Ca is much stronger than that of other metal ions.
The cultural conditions and composition of media for optimal production of amylase by B. amyloliquefaciens P-001 has been developed in this study. Enzyme synthesis was affected by carbon and nitrogen sources and maximal activity was attained with inorganic than organic nitrogen sources. The optimum enzyme production by the bacterial isolate was found at 42°C, whereas maximum enzyme activity was observed at 60°C which could make the enzyme from B. amyloliquefaciens P-001 more suitable for future use in various industries. It can be concluded that, B. amyloliquefaciens P-001 can be a potential producer of extracellular amylase which could find applications in industry. Due to the importance of these findings, further studies need be carried on in order to commercialize the production process.
The bacterial culture Bacillus amyloliquefacience P-001 was obtained from the Microbial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Dhaka. It was maintained on nutrient agar medium. The organism was maintained at 4°C in refrigerator for routine laboratory use. For the long term preservation, the log phage growth bacteria were maintained in 15% glycerol broth at -20°C.
Plate assay method
The Bacillus isolates were tested for amylase activity by employing zone clearing technique Atlas et al. (1995) using starch agar medium. The inoculated plates were incubated at 37°C for two days. After incubation, the zone of hydrolysis of starch was detected by flooding the plates with iodine solution. The development of blue colour indicated the presence of starch, while the areas around the hydrolytic bacteria appeared clear.
Preparation of seed culture
Vegetative inoculums were used in the present studies. Fifty millilitre of inoculums medium containing nutrient broth 13 g/l, pH 7.4 ± 0.2 was transferred to a 100 ml conical flask and cotton plugged. It was sterilized in an autoclave at 15 lbs/inch2 pressure at 121°C for 20 min. After cooling to room temperature, a loopful of freshly grown culture was aseptically transferred to it. The flask was incubated overnight at 37°C and 150 rpm in a rotary shaking incubator.
Enzyme production in shake flask cultures
The enzyme production was carried out in the basal Asgher et al. (2007) medium containing 0.1% KH2PO4, 0.25% Na2 HPO4, 0.1% NaCl, 0.2% (NH4)2SO4,0.005% MgSO4 .7H2O, 0.005% CaCl2, 0.2% tryptone and 1% soluble starch. 1 ml of 24 hours grown inoculums were cultivated in 250-ml Erlenmeyer flasks containing 100 ml of medium with an initial pH 7.0. The cultures were shaken at 150 rpm in a orbital shaker incubator at 37°C for at least 48 h unless otherwise stated. After the incubation, the fermented broth was centrifuged in a refrigerated centrifuge machine at 8000 rpm for 15 minutes at 4°C.
Amylase was determined by using soluble starch, 1% (w/v), as substrate in 0.05 M Sodium phosphate buffer (pH 6.5) essentially according to Gomes et al. (2001). The reaction mixture containing 1.8 ml substrate solution and 0.2 ml suitably diluted enzyme solution was incubated at 50°C for 10 min. The reaction was stopped by adding 3 ml dinitrosalicylic acid (DNS). The reducing sugar released was determined by the method ofMiller (1959). The absorbance was measured at 540 nm with spectrophotometer (Jenway 6305, USA). One unit (U) of enzyme activity is defined in all cases as the amount of enzyme releasing 1 μg of reducing sugar as maltose per minute, under assay conditions.
Soluble protein estimation
Extracellular soluble protein in culture filtrate was estimated by the Lowry’s method using bovine serum albumin (BSA) used as Standard Lowry et al. (1951). 2 ml of analytical reagent was added to 0.2 ml suitably diluted test samples (enzyme solution). The mixture was mixed well and allowed to stand for 10 min at 50°C. Then 0.2 ml of the folin-ciocalteau reagent was added and shaken to mix well and incubated at room temperature for about 30 min. Optical density of the reaction mixture was measured at 600 nm, against a blank prepared with 0.2 ml buffer. A standard curve was constructed with each experiment using bovine serum albumin as a known protein. The amount of the soluble protein was calculated from the standard curve of as mg protein per ml of test samples.
Abate CM, Castro NGR, Sineriz F, Callieri DAS: Production of amylolytic enzymes by Bacillus amyloliquefaciens in pure culture and in co-culture with Zymomonas mobilis. Biotechnol Lett 1999, 21: 249-252. 10.1023/A:1005477505683
Aehle W, Misset O: Enzymes for industrial applications. In Biotechnology. 2nd edition. Edited by: Rehm HJ, Reed G. Wiley-VCH, Germany; 1999.
Akcan N: High level production of extracellular α-amylase from Bacillus licheniformis ATCC 12759 in submerged fermentation. Rom Biotechnol Lett 2011, 16(6):6833-6840.
Asgher M, Asad MJ, Rahman SU, Legge RL: A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing. J Food Eng 2007, 79: 950-955. 10.1016/j.jfoodeng.2005.12.053
Atlas RM, Parks LC, Brown AE: Laboratory Manual of Experimental Microbiology. Mosby-Year Book Inc., St Louis; 1995.
Aygan A, Arikan B, Korkmaz H, Dincer S, Colak O: Highly thermostable and alkaline alpha amylase from halotolerant-alkaliphilic Bacillus sp. AB68. Braz J Microbiol 2008, 39: 547-553.
Azad MA, Bae JH, Kim JS, Lim JK, Song KS, Shin BS, Kim HR: Isolation and characterization of a novel thermostable alpha-amylase from Korean pine seeds. N Biotechnol 2009, 26: 143-149. 10.1016/j.nbt.2009.09.006
Banargee R, Bhattacharya BC: Extracellular alkaline protease of newly isolated Rhizopus oryzae. Biotechnol lett 1992, 14: 301-304. 10.1007/BF01022328
Bandyopadhyay A, Pal SC, Sen SK: Alpha amylase production in lactose medium by Bacillus circulanse ACB. Microbiologia 1993, 9(2):142-148.
Bose K, Das D: Thermostable α-amylase production using B. licheniformis NRRL B1438. Indian J Exp Biol 1996, 34: 1279-1282.
Bozic N, Ruiz J, Lopez-Santin J, Vujcic Z: Optimization of the growth and α-amylase production of Bacillus subtilis IP 5832 in shake flask and laboratory fermenter batch cultures. J Serb Chem Soc 2011, 76(7):965-972. 10.2298/JSC101010098B
Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman G: Enzymatic properties of a novel thermophilic, alkaline and chelator resistant amylase from an alkalophilic Bacillus sp. Isolate ANT-6. Process Biochem 2003, 38: 1397-1403. 10.1016/S0032-9592(03)00037-2
Castro PML, Hayter PM, Ison AP, Bull AT: Application of statistical design to the optimization of culture mediumfor recombinant interferon-gamma production by Chinese hamsterovary cells. Appl Microbiol Biotechnol 1992, 38(1):84-90.
Castro GR, Ferrero MA, Mendez BS, Sineriz F: Screening and selection of bacteria with high amylolytic activity. Acta Biotechnol 1993, 13: 197-201. 10.1002/abio.370130220
Chung YC, Kobayashi T, Kanai H, Akiba T, Kudo T: Purification and properties of extracellular amylase from the hyperthermophilic Archaeon Thermococcus profundus DT 5432. Appl Environ Microbiol 1995, 61(4):1502-1506.
Coleman G, Elliott WH: Studies on α-amylase formation by Bacillus subtilis. Biochem J 1962, 83(2):256-263.
Das K, Doley R, Mukherjee AK: Purification and biochemical characterization of thermostable, alkaliphilic, extracellular α-amylase from Bacillus subtilis DM-03, a strain isolated from the traditional food of India. Biotechnol Appl Biochem 2004, 40: 291-298. 10.1042/BA20040034
Das S, Singh S, Sharma V, Soni ML: Biotechnological applications of industrially important amylase enzyme. Inter J Phar Bio Sci 2011, 2(1):486-496.
Djekrif-Dakhmouche S, Gheribi-Aoulmi Z, Meraihi Z, Bennamoun L: Application of a statistical design to the optimization of culture medium for α-amylase production by Aspergillus niger ATCC 16404 grown on orange waste powder. J Food Eng 2005, 73: 190-197.
El-Tayeb O, Mohammed F, Hashem A, Abdullah M: Optimization of the industrial production of bacterial alpha amyalse in Egypt. IV. Fermentor production and characterization of the enzyme of two strains of Bacillus subtilis and Bacillus amyloliquefaciens. Afr J Biotechnol 2007, 7(24):4521-4536.
Ferrari E, Jarnagin AS, Schmidt BF: Commercial Production of Extracellular Enzymes. In Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics. Edited by: Sonenheim AL, Hoch JA, Losick R. American Society for Microbiology Press, Washington DC; 1993.
Gangadharan D, Sivaramakrishnan S, Namboothiri KM, Pandey A: Solid culturing of Bacillus amyloliquefaciens for -amylase production. Food Technol Biotechnol 2006, 44(2):269-274.
Gomes I, Sultana M, Uddin K, Gomes J, Steiner W, Gomes DJ: Nutrient composition and fermentation conditions for α-amylase production by Bacillus amyloliquefaciens. Bangladesh J Microbiol 2001, 18(2):141-150.
Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B: Microbial α-amylases: a biotechnological prospective. Process Biochem 2003, 38: 1599-1616. 10.1016/S0032-9592(03)00053-0
Haq I, Ashraf H, Qadeer MA, Iqbal J: Pearl millet, a source of alpha amylase production by Bacillus licheniformis. Bioresour Technol 2005, 96(10):1201-1204. 10.1016/j.biortech.2004.09.012
Haq I, Ali S, Javed MM, Hameed U, Saleem A, Adnan F, Qadeer MA: Production of alpha amylase from a randomly induced mutant strain of Bacillus amyloliquefaciens and its application as a desizer in textile industry. Pak J Bot 2010, 42(1):473-484.
Harwood CR: Bacillus subtilis and its relatives: Molecular, biological and industrial workhorses. Trends Biotechnol 1992, 10: 247-256.
Hillier P, Wase DAJ, Emery AN, Solomons GL: Instability of α-amylase production and morphological variation in continuous culture of Bacillus amyloliquefaciens is associated with plasmid loss. Process Biochem 1997, 32: 51-59. 10.1016/S0032-9592(96)00048-9
Huang H, Ridway D, Gu T, Moo-Young M: A segregated model for heterologous α-amylase production by Bacillus subtilis. Enzyme Microb Technol 2003, 32: 407-413. 10.1016/S0141-0229(02)00312-5
Kathiresan K, Manivannan S: α-amylase production by Penicillium fellutanum isolated from mangrove rhizospheric soil. Afr J Biotechnol 2006, 5(10):829-832.
Kaur P, Vyas A: Characterization and optimal production of alkaline α-amylase from Bacillus sp. DLB 9. Afr J Microbiol Res 2012, 6(11):2674-2681.
Kumar CG, Takagi H: Microbial alkaline protease: from a bioindustrial viewpoint. Biotechnol Adv 1999, 17(7):561-594. 10.1016/S0734-9750(99)00027-0
Kundu AK, Das S, Gupta TK: Influence of culture and nurtitional conditions on the production of amylase by the submerged culture of Aspergillus oryzae. J Ferment Technol 1973, 51: 142-150.
Lin LL, Hsu WH: A gene encoding for an alpha-amylase from thermophilic Bacillus sp. strain TS-23 and its expression in Escherichia coli. J Appl Microbiol 1997, 82: 325-334. 10.1046/j.1365-2672.1997.00364.x
Lin LL, Tsau MR, Chu WS: General characteristics of thermostable pullulanases and amylases from an alkalophilic Bacillus sp. TS-23. Appl Microbiol Biotechnol 1994, 42: 51-56. 10.1007/BF00170224
Lin LL, Chyau CC, Hsu WH: Production and properties of a raw-starch-degrading amylase from thermophilic and alkaliphilic Bacillus sp. TS-23. Biotechnol Appl Biochem 1998, 28: 61-68.
Lowry OH, Rosbrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951, 193(1):265-275.
Magee RJ, Kosaric N: The microbial production of 2,3-butanediol. Adv Appl Microbiol 1987, 32: 89-161.
Mahmood S, Rahman SR: Production and partial characterization of extracellular α-amylase by Trichoderma viride. Bangladesh J Microbiol 2008, 25(2):99-103.
Mendu DR, Ratnam BVV, Purnima A, Ayyanna C: Affinity chromatography of α-amylase from Bacillus licheniformis. Enzyme Microb Technol 2005, 37: 712-717. 10.1016/j.enzmictec.2005.04.015
Mielenz JR: Bacillus stearothermophilus contains a plasmid-borne gene for alpha-amylase. Proc Natl Acad Sci 1983, 80: 5975-5979. 10.1073/pnas.80.19.5975
Miller GL: Use of dinitrosalisylic acid reagent for determination of reducing sugar. Anal Chem 1959, 31(3):426-428. 10.1021/ac60147a030
Mishra S, Behera N: Amylase activity of starch degrading bacteria is isolated from soil receiving kitchen wastes. Afr J Biotechnol 2008, 7: 3326-3331.
Mishra S, Noronha SB, Kumar GKS: Increase in enzyme productivity by induced oxidative stress in Bacillus subtilis cultures and analysis of its mechanism using microarray data. Process Biochem 2005, 40: 1863-1870. 10.1016/j.procbio.2004.06.055
Narang S, Satyanarayana T: Thermostable alpha-amylase production by an extreme thermophilic Bacillus thermooleovorans. Lett Appl Microbiol 2001, 32(1):31-35. 10.1046/j.1472-765x.2001.00849.x
Nusrat A, Rahman SR: Comparative studies on the production of extracellular α-amylase by three mesophilic Bacillus isolates. Bangladesh J Microbiol 2007, 24(2):129-132.
Okalo BN, Ezeogu LI, Ebisike CO: Raw starch digesting amylase from Thermoactinomyces thalophilus F13. World J Microb Biotechnol 1996, 12: 637-638. 10.1007/BF00327728
Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R: Advances in microbial amylases. Biotechnol Appl Biochem 2000, 31: 135-152. 10.1042/BA19990073
Prakash B, Vidyasagar M, Madhukumar MS, Muralikrishna G, Sreeramulu K: Production, purification and characterization of two extremely halotolerant, thermostable, and alkali-stable α-amylases from Chromohalobacter sp. TVSP 101. Process Biochem 2009, 44: 210-215. 10.1016/j.procbio.2008.10.013
Rajagopalan G, Krishnan C: Alpha-amylase production from catabolite derepressed Bacillus subtilis KCC103 utilizing sugarcane bagasse hydrolysate. Bioresour Technol 2008, 99(8):3044-3050. 10.1016/j.biortech.2007.06.001
Riaz N, Haq IU, Qadeer MA: Characterization of α-amylase by Bacillus subtilis. Int J Agr Biol 2003, 5(3):249-252.
Schallmey M, Singh A, Ward OP: Developments in the use of Bacillus species for industrial production. Can J Microbiol 2004, 50: 1-17. 10.1139/w03-076
Sexana R, Dutt K, Agarwal L, Nayyar P: A highly thermostable and alkaline amylase from a Bacillus sp. PN5. Bioresour Technol 2007, 98(2):260-265. 10.1016/j.biortech.2006.01.016
Sharma N, Vamil R, Ahmad S, Agarwal R: Effect of different carbon and nitrogen sources on α-amylase production from Bacillius amyloliquefaciens. Int J Pharm Sci Res 2012, 3(4):1161-1163.
Sumrin A, Ahmad W, Ijaz B, Sarwar MT, Gull S, Kausar H, Shahid I, Jahan S, Asad S, Hussain M, Riazuddin S: Purification and medium optimization of α-amylase from Bacillus subtilis 168. Afr J Biotechnol 2011, 10(11):2119-2129.
Swain MR, Kar S, Padmaja G, Ray RC: Partial characterization and optimization of production of extracellular α-amylase from Bacillus subtilis isolated from culturable cow dung microflora. Pol J Microbiol 2006, 55(4):289-296.
Swamy MV, Seenayya G: Thermostable pullulanase and α-amylase activity from Clostridium thermosulfurogenes SV9-optimization of culture conditions for enzyme production. Process Biochem 1996, 31: 157-162. 10.1016/0032-9592(95)00034-8
Syu MJ, Chen YH: A study on the α-amylase fermentation performed by Bacillus amyloliquefaciens. Chem Eng J 1997, 65: 237-247. 10.1016/S1385-8947(97)00020-X
Tonkova A: Effect of glucose and citrate on α-amylase production in Bacillus licheniformis. J Basic Microbiol 1991, 31: 217-222. 10.1002/jobm.3620310310
Wanderley KJ, Torres FAG, Moraes LMP, Ulhoa CJ: Biochemical characterization of α-amylase from the yeast Cryptococcus flavus. FEMS Microbiol Lett 2004, 231(2):165-169. 10.1016/S0378-1097(03)00955-8
Wolfgang A: Enzyme in industry: Production and Applications. Wiley-VCH, Weinheim; 2007.
The research was supported by National Institute of Biotechnology, Savar, Dhaka, Bangladesh.
The authors declare that they have no competing interests.
PKS planned the work that led to the manuscript; PD produced and analyzed the experimental data; SA and KM participated in the interpretation of the results; PD, KM, SMAS and PKS wrote the paper. All authors read and approved the final manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Deb, P., Talukdar, S.A., Mohsina, K. et al. Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001. SpringerPlus 2, 154 (2013). https://doi.org/10.1186/2193-1801-2-154
- Bacillus amyloliquefaciens
- Extracellular amylase
- Shake flask culture
- Production optimization