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Table 1 Effect of culture conditions for extracellular amylase production from Bacillus amyloliquefaciens P-001 in shake-flask cultivations

From: Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001

Culture condition Amylase activity (U/ml) (Mean ± SE) Relative activity (%) Total soluble protein (mg/ml) (Mean ± SE)
Initial pH    
  6.0 17.25±0.53 48.13 0.87±0.01
  6.5 22.65±0.58 63.20 1.45±0.03
  7.0 27.71±0.45 77.32 1.03±0.04
  7.5 28.09±0.93 78.38 2.76±0.03
  8.0 28.92±0.10 80.69 3.01±0.04
  8.5 31.01±0.94 86.52 2.69±0.02
  9.0 35.84±0.17 100.00 2.84±0.03
  9.5 25.39±0.31 70.84 2.73±0.02
Incubation temperature (°C)    
  32 25.75±0.53 46.88 3.34±0.05
  35 37.14±0.89 67.61 2.35±0.06
  37 39.20±0.19 71.36 2.09±0.05
  40 53.85±0.40 98.03 1.79±0.02
  42 54.93±0.18 100.00 1.18±0.03
  45 30.33±0.59 55.22 2.40±0.04
Incubation period (hr)    
  24 10.49±0.19 38.24 4.15±0.04
  48 27.43±0.08 100.00 3.97±0.07
  72 26.28±0.21 95.81 3.84±0.05
  96 24.04±0.07 87.64 3.23±0.06
Inoculums volume (%)    
  1.0 39.25±0.20 100.00 -
  1.5 35.92±0.27 91.52 -
  2.0 34.46±0.17 87.80 -
  2.5 31.18±0.27 79.44 -
  3.0 30.97±0.18 78.90 -
  3.5 28.38±0.18 72.31 -
  1. Data represent as mean ± standard error (SE) for three replicates.