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Table 1 Effect of culture conditions for extracellular amylase production from Bacillus amyloliquefaciens P-001 in shake-flask cultivations
| Culture condition | Amylase activity (U/ml) (Mean ± SE) | Relative activity (%) | Total soluble protein (mg/ml) (Mean ± SE) |
|---|---|---|---|
| Initial pH | |||
| 6.0 | 17.25±0.53 | 48.13 | 0.87±0.01 |
| 6.5 | 22.65±0.58 | 63.20 | 1.45±0.03 |
| 7.0 | 27.71±0.45 | 77.32 | 1.03±0.04 |
| 7.5 | 28.09±0.93 | 78.38 | 2.76±0.03 |
| 8.0 | 28.92±0.10 | 80.69 | 3.01±0.04 |
| 8.5 | 31.01±0.94 | 86.52 | 2.69±0.02 |
| 9.0 | 35.84±0.17 | 100.00 | 2.84±0.03 |
| 9.5 | 25.39±0.31 | 70.84 | 2.73±0.02 |
| Incubation temperature (°C) | |||
| 32 | 25.75±0.53 | 46.88 | 3.34±0.05 |
| 35 | 37.14±0.89 | 67.61 | 2.35±0.06 |
| 37 | 39.20±0.19 | 71.36 | 2.09±0.05 |
| 40 | 53.85±0.40 | 98.03 | 1.79±0.02 |
| 42 | 54.93±0.18 | 100.00 | 1.18±0.03 |
| 45 | 30.33±0.59 | 55.22 | 2.40±0.04 |
| Incubation period (hr) | |||
| 24 | 10.49±0.19 | 38.24 | 4.15±0.04 |
| 48 | 27.43±0.08 | 100.00 | 3.97±0.07 |
| 72 | 26.28±0.21 | 95.81 | 3.84±0.05 |
| 96 | 24.04±0.07 | 87.64 | 3.23±0.06 |
| Inoculums volume (%) | |||
| 1.0 | 39.25±0.20 | 100.00 | - |
| 1.5 | 35.92±0.27 | 91.52 | - |
| 2.0 | 34.46±0.17 | 87.80 | - |
| 2.5 | 31.18±0.27 | 79.44 | - |
| 3.0 | 30.97±0.18 | 78.90 | - |
| 3.5 | 28.38±0.18 | 72.31 | - |
