|
Culture condition
|
Amylase activity (U/ml) (Mean ± SE)
|
Relative activity (%)
|
Total soluble protein (mg/ml) (Mean ± SE)
|
|---|
|
Initial pH
| | | |
|
6.0
|
17.25±0.53
|
48.13
|
0.87±0.01
|
|
6.5
|
22.65±0.58
|
63.20
|
1.45±0.03
|
|
7.0
|
27.71±0.45
|
77.32
|
1.03±0.04
|
|
7.5
|
28.09±0.93
|
78.38
|
2.76±0.03
|
|
8.0
|
28.92±0.10
|
80.69
|
3.01±0.04
|
|
8.5
|
31.01±0.94
|
86.52
|
2.69±0.02
|
|
9.0
|
35.84±0.17
|
100.00
|
2.84±0.03
|
|
9.5
|
25.39±0.31
|
70.84
|
2.73±0.02
|
|
Incubation temperature (°C)
| | | |
|
32
|
25.75±0.53
|
46.88
|
3.34±0.05
|
|
35
|
37.14±0.89
|
67.61
|
2.35±0.06
|
|
37
|
39.20±0.19
|
71.36
|
2.09±0.05
|
|
40
|
53.85±0.40
|
98.03
|
1.79±0.02
|
|
42
|
54.93±0.18
|
100.00
|
1.18±0.03
|
|
45
|
30.33±0.59
|
55.22
|
2.40±0.04
|
|
Incubation period (hr)
| | | |
|
24
|
10.49±0.19
|
38.24
|
4.15±0.04
|
|
48
|
27.43±0.08
|
100.00
|
3.97±0.07
|
|
72
|
26.28±0.21
|
95.81
|
3.84±0.05
|
|
96
|
24.04±0.07
|
87.64
|
3.23±0.06
|
|
Inoculums volume (%)
| | | |
|
1.0
|
39.25±0.20
|
100.00
|
-
|
|
1.5
|
35.92±0.27
|
91.52
|
-
|
|
2.0
|
34.46±0.17
|
87.80
|
-
|
|
2.5
|
31.18±0.27
|
79.44
|
-
|
|
3.0
|
30.97±0.18
|
78.90
|
-
|
|
3.5
|
28.38±0.18
|
72.31
|
-
|
- Data represent as mean ± standard error (SE) for three replicates.
