In vitro and in vivo evaluation of protein quality of enzymatic treated feather meals
© The Author(s) 2016
Received: 26 January 2016
Accepted: 19 June 2016
Published: 4 July 2016
Feeding trials were designed to evaluate the nutritive value of feather meal treated by K6 and K82 keratinase. There were five treatments in feather meal preparation: CFM (non-enzymatically treated feather meal), K6FM (K6 keratinase treated feather meal), K82FM (K82 keratinase treated feather meal), K6:K82FM [K6 and K82 keratinase (5:1) treated feather meal] and CMFM (commercial enzyme treated feather meal). The pepsin digestibility of CFM (70 %) and CMFM (68 %) was significantly higher than K6FM (60 %), K82FM (61 %) and K6:K82FM (63 %). Total amino acid content of K82FM (89.65/100 g) was the highest compared with the other treatments. The nutrient digestibility of the feather meals was determined for broiler chicks between 21 and 27 days old. The apparent nitrogen retention of K82FM (85.82 %) and K6FM (77.31 %) was significantly higher than K6:K82FM (55.42 %), CMFM (45.70 %) and CFM (48.16 %). The apparent metabolisable energy (AMEn) was not significantly different between the feather meal treatments, although K82FM, K6FM and K6:K82FM showed AMEn higher than CMFM and CFM. The results indicated that both K6 and K82 keratinase had a positive effect on the protein quality of the feather meal produced by the enzymatic–hydrothermal method.
KeywordsBacillus Feather meal Keratinase Broiler
Feathers are produced in large amounts as by-product of poultry processing plant worldwide. The feather is 90 % keratin protein and the accumulation of feathers in the environment results in pollution and feather protein wastage (Belarmino et al. 2012; Gopinath et al. 2015; Gousterova et al. 2005; Onifade et al. 1998). Therefore, utilization of biological by-product as livestock feed is an accepted practice, which reduces costs both in terms of waste disposal and meat production from livestock (Grazziotin et al. 2006). Feathers have elevated keratin content and the use of this protein source should be considered. Traditional ways to degrade feathers such as alkali hydrolysis and steam pressure cooking not only destroy the amino acids, but also require large amounts of energy (Papadopoulos et al. 1986; Tiwary and Gupta 2012). The biodegradation of the feathers by keratinase from microorganisms could be a cost effective alternative. Keratinase and related products have many applications (Brandelli et al. 2010; Gupta and Ramnani 2006; Gupta et al. 2013). For example, the feather hydrolysates of Bacillus licheniformis PWD-1 and Vibrio sp. strain kr2 (Williams et al. 1991; Grazziotin et al. 2006) can be used as feed additives, while the keratinase from Bacillus subtilis S14 and Brevibacillus brevis US575 exhibits remarkable dehairing capabilities in the leather processing industry (Macedo et al. 2005; Jaouadi et al. 2013). Keratinase is very important for the utilisation of feather meal. The properties of keratinolysis are widespread in the microbial world. However, only few of these properties have been commercially exploited. Keratinases from Bacillus sp. particularly B. licheniformis and B. subtilis have been extensively studied for their efficiency in terms of feather degradation (Manczinger et al. 2003; Mazotto et al. 2011; Thys et al. 2004). A feather-degrading B. licheniformis KUB-K0006 and B. pumilus KUB-K0082 were discovered and isolated by Nitisinprasert and Kaewsompong (1997). These aerobic bacterial isolates possessed effective keratinase, with high feather digestibility at wide pH ranges and high temperatures of up to 50 °C. The enzyme keratinases were purified and characterised as a serine protease (Nitisinprasert et al. 1999; Titapoka 2003). Keratinases from B. licheniformis KUB-K0006 (K6 keratinase) and B. pumilus KUB-K0082 (K82 keratinase) displayed different abilities of feather digestion and quantities of amino acid released. K6 keratinase is endo-acting, whereas K82 keratinase is an exo-acting enzyme (Nitisinprasert et al. 1999). The aims of this study were to evaluate the effect of K6 and K82 keratinase on feather meal quality and nutrient digestibility at industrial scale production levels of broiler chicks.
Results and discussion
Characteristics of feather meals from industrial scale production
Proximate composition and pepsin digestibility of feather meal CFM, K6FM, K82FM, K6:K82FM and CMFM
Fat ball (kg)
Crude fat (%)
Crude protein (%)
Energy (Cal/100 g)
Pepsin digestibility (%)
Amino acid composition of feather meal CFM, K6FM, K82FM, K6:K82FM and CMFM
Amino acid (g/100 g)
Total amino acids
Effect of feather meal on nutrient digestion of broiler chicks
Apparent nitrogen retention (ANR), apparent fat digestibility (AFD) and apparent metabolisable energy (AMEn) in feed samples
Apparent nitrogen retention (%)
Apparent fat digestibility (%)
Apparent metabolisable energy (N-corrected) (kcal/kg)
Diet 1 (control)
Diet 2 (K6FM)
Diet 3 (K82FM)
Diet 4 (K6:K82FM)
Diet 5 (CMFM)
Pool ± SE
There were no significant differences in the AFD and apparent metabolisable energy among the treatments (P > 0.05). However, Diet 3 (K82FM) showed the highest AMEn, 440 kcal/kg higher than Diet 1 (CFM). This indicated that the feather meal prepared by keratinase at high temperature and pressure increased the metabolisable energy levels of the broilers. The results revealed that K82FM was a good alternative protein source, with high levels of AFD and AMEn.
In vivo digestibility, metabolisable energy and protein (nitrogen) retention of the enzymatic treated feather meal showed an inverse relationship with in vitro pepsin digestibility. However, the amino acid content of the feather meal with enzyme treatments was higher than without the treatment (Table 2). Papadopoulos (1987) concluded that pepsin digestibility should not be considered in the evaluation of feather meal as a poultry feed. Although the trends in values were similar, there was poor correlation between in vitro and in vivo tests of protein quality, therefore care should be taken when comparing reports in which different methods of testing have been used.
The feather meals prepared from hydrolysis reactions using concentrated crude K6 and K82 keratinase were tested for nutrient digestibility in the broiler chicks. Results showed higher ANR for K6FM and K82FM in vivo. Nevertheless, crude protein and total amino acid contents of K82FM were highest compared against the other feather meal treatments. The value of pepsin digestible protein (PDP) of the feather meal produced from the commercial enzyme (CMFM) was the highest, but K6FM and K82FM were found to improve the ANR of the chicks better than CMFM. There were no significant differences in apparent metabolisable energy among the feather meal treatments, however K6 and K82 keratinase feather meal treatments showed higher AMEn than the commercial enzyme feather meal treatment (180 kcal/kg). Therefore, the K6 and K82 keratinase showed potential use for industrial feather meal production.
K6 keratinase and K82 keratinase were prepared from B. licheniformis KUB-K0006 and B. pumilus KUB-K0082, respectively. The fermentation medium contained (g/l) NH4Cl (0.5), NaCl (0.5), K2HPO4.3H2O (0.354), KH2PO4 (0.4), MgCl2·6H2O (0.24) and feather meal (10 % w/v), with initial pH at 7.5. The production was performed in a 200 l fermenter (FM-300A, B.E. Marubishi, Japan), aeration rate 1.0 l/min, agitation speed 250 rpm at a temperature of 37 °C for 24 h. Inoculation volume was 5 %. The cell-free media were concentrated by membrane ultrafiltration with 30 kDa molecular weight cut off (Sartorius model SM 17546, Germany). The activity of crude concentrated K6 and K82 keratinase was measured by the hydrolysis of milled feather (Lin et al. 1992).
Feather meal production
Feather meals were prepared at industrial scale production (3.8 tonnes feather/batch). The concentrated crude K6 keratinase, K82 keratinase, K6:K82 (5:1) and commercial enzyme were used in the feather meal production. A total of 3.8 tonnes of native feathers were collected from the slaughter house of B. Foods Product International Co., Ltd. (Thailand) and then transferred into a cooker/drier with keratinase enzyme (493,600 U/batch). The feathers were incubated at 60 °C for 1 h and then autoclaved at 130 °C for 20 min. After hydrolysis, the feathers were dried to 10 % moisture content (maximum value) and hammer milled to obtain 3 mm particle size. The feather meal was stored in a silo. The samples were analysed; moisture (ISO 1999), crude fat (AOAC 2000), ash, protein, pepsin digestibility and amino acid profiles (AOAC 2005) at Betagro Science Center Co., Ltd. (Thailand).
Diet composition and nutrient content of corn soybean meal diet
Soybean meal, 440 g/kg CP
Mono-dicalcium phosphate (MCP)
Premixa (vitamin and mineral)
ME for poultry (kcal/kg)
Methionine + cysteine
Chickens and management
The study protocol was approved by the Animal Ethics Committee of Kasetsart University, Thailand. One hundred and twenty male broiler chicks aged 21 days (Ross 308, Betagro Agro-Group Public Co. Ltd.), were randomly allocated to 20 metabolic cages. Each treatment was replicated four times with six birds per cage. The birds were housed in a room maintained at a controlled temperature of 25 °C with ventilation and lighting. Feed and water were provided to the chicks for ad libitum intake throughout the experiment. Test diets were provided for the 21 day old chicks when they were placed in the cages. After 4 days of experimental diet feeding (adjusting period), all the feeders were removed and all the excreta trays were cleaned. The amount of remaining feed was recorded and the feeders were then reinstalled. This was considered as the starting point of the feed intake and excreta collection period. During the 72 h collection period, excreta samples of 200 g for each replicate were collected once daily at 8:00 a.m. All excreta trays were cleaned at 4:00 pm in the evening to prevent contamination. The wet excreta was adjusted to pH 4.0 using 6 N sulphuric acid and then stored at −4 °C until required for testing. At the end of the collection period the samples were defrosted, homogenized, sun dried and 200 g aliquots were dried in an oven at 70 °C to constant weight. Dried excreta and feed samples were milled and analysed for moisture, gross energy (GE), crude protein and crude fat (AOAC 1990). Chromic oxide was measured following the method of Bolin et al. (1952). ANR, AFD and apparent metabolisable energy corrected for nitrogen (AMEn) were calculated according to the method of Hill et al. (1960).
The data were subjected to analysis of variance (ANOVA) arranged in completely randomized design (CRD). Significant differences between treatment groups were detected by Duncan’s multiple range test and contrast comparison analysis (SAS, 2003). Means were compared and considered significant when P < 0.05.
WE, AP, TU, RT, SN and SK conceived, designed of the study and analyzed the data. WE also drafted the manuscript. All authors read and approved the final manuscript.
This research was financially supported by the Royal Golden Jubilee (RGJ) Grant, The Thailand Research Fund (TRF), Kasetsart University Research and Development Institute (KURDI), and the Office of the Higher Education Commission, Thailand. We also thank Betagro Science Center Co., Ltd. and B. Foods Product International Co., Ltd. for technical support.
The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, ArlingtonGoogle Scholar
- AOAC (2000) Official methods of analysis of AOAC International. Association of Official Analytical Chemists, GaithersburgGoogle Scholar
- AOAC (2005) Official methods of analysis of AOAC International. AOAC International, GaithersburgGoogle Scholar
- Belarmino D, Ladchumananandasivam R, Belarmino L, Pimentel J, Rocha B, Galvão A, Andrade S (2012) Physical and morphological structure of chicken feathers (keratin biofiber) in natural, chemically and thermally modified forms. Mater Sci Appl 3:887–893Google Scholar
- Bolin DW, King RP, Klosterman EW (1952) A simplified method for the determination of chromic oxide (Cr2O3) when used as an index substance. Science 116(3023):634–635View ArticleGoogle Scholar
- Brandelli A, Daroit DJ, Riffel A (2010) Biochemical features of microbial keratinases and their production and applications. Appl Microbiol Biotechnol 85(6):1735–1750View ArticleGoogle Scholar
- Eaksuree W, Prachayakiti A, Taharnklaew R, Haltrich D, Nitisinprasert S, Keawsompong S (2016) Keratinase Fermentation by Bacillus licheniformis KUB-K0006. VRU Res Dev J Sci Technol 11(2)Google Scholar
- Gopinath SCB, Anbu P, Lakshmipriya T, Tang T-H, Chen Y, Hashim U, Ruslinda AR, Arshad MKM (2015) Biotechnological aspects and perspective of microbial keratinase production. Biomed Res Int 2015:10View ArticleGoogle Scholar
- Gousterova A, Braikova D, Goshev I, Christov P, Tishinov K, Vasileva-Tonkova E, Haertlé T, Nedkov P (2005) Degradation of keratin and collagen containing wastes by newly isolated thermoactinomycetes or by alkaline hydrolysis. Lett Appl Microbiol 40(5):335–340View ArticleGoogle Scholar
- Grazziotin A, Pimentel FA, de Jong EV, Brandelli A (2006) Nutritional improvement of feather protein by treatment with microbial keratinase. Anim Feed Sci Tech 126(1–2):135–144View ArticleGoogle Scholar
- Gupta R, Ramnani P (2006) Microbial keratinases and their prospective applications: an overview. Appl Microbiol Biotechnol 70(1):21–33View ArticleGoogle Scholar
- Gupta R, Rajput R, Sharma R, Gupta N (2013) Biotechnological applications and prospective market of microbial keratinases. Appl Microbiol Biotechnol 97(23):9931–9940View ArticleGoogle Scholar
- Hill FW, Anderson DL, Renner R, Carew LB (1960) Studies of the metabolizable energy of grain and grain products for chickens. Poult Sci 39(3):573–579View ArticleGoogle Scholar
- ISO (1999) Animal feeding stuffs—determination of moisture and other volatile matter content. Standard 6496:1999. International Organisation for Standardisation (ISO), GenevaGoogle Scholar
- Jaouadi NZ, Rekik H, Badis A, Trabelsi S, Belhoul M, Yahiaoui AB, Aicha HB, Toumi A, Bejar S, Jaouadi B (2013) Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities. PLoS ONE 8(10):e76722View ArticleGoogle Scholar
- Kim WK, Patterson PH (2000) Nutritional value of enzyme- or sodium hydroxide-treated feathers from dead hens. Poult Sci 79(4):528–534View ArticleGoogle Scholar
- Lin X, Lee CG, Casale ES, Shih JC (1992) Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl Environ Microbiol 58(10):3271–3275Google Scholar
- Macedo AJ, da Silva WO, Gava R, Driemeier D, Henriques JA, Termignoni C (2005) Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities. Appl Environ Microbiol 71(1):594–596View ArticleGoogle Scholar
- Manczinger L, Rozs M, Vágvölgyi C, Kevei F (2003) Isolation and characterization of a new keratinolytic Bacillus licheniformis strain. World J Microbiol Biotechnol 19(1):35–39View ArticleGoogle Scholar
- Mazotto AM, Coelho RR, Cedrola SM, de Lima MF, Couri S, Paraguai de Souza E, Vermelho AB (2011) Keratinase production by three Bacillus spp. using feather meal and whole feather as substrate in a submerged fermentation. Enzyme Res. Article ID 523780Google Scholar
- Nitisinprasert S, Kaewsompong S (1997) Screening and characterization of microorganisms for feather degradation from various sources in Thailand. J Sci Khonkaen Univ 25(1):65–75Google Scholar
- Nitisinprasert S, Pornwirun W, Keawsompong S (1999) Characterizations of two bacterial strains showing high keratinase activities and their synergism in feather degradation. Kasetsart J (Nat Sci) 33:191–199Google Scholar
- Onifade AA, Al-Sane NA, Al-Musallam AA, Al-Zarban S (1998) A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour Technol 66(1):1–11View ArticleGoogle Scholar
- Papadopoulos MC (1987) In vitro and in vivo estimation of protein quality of laboratory treated feather meal. Bio Waste 21(2):143–148View ArticleGoogle Scholar
- Papadopoulos MC, El Boushy AR, Roodbeen AE, Ketelaars EH (1986) Effects of processing time and moisture content on amino acid composition and nitrogen characteristics of feather meal. Ann Feed Sci Technol 14(3–4):279–290View ArticleGoogle Scholar
- Thys RC, Lucas FS, Riffel A, Heeb P, Brandelli A (2004) Characterization of a protease of a feather-degrading microbacterium species. Lett Appl Microbiol 39(2):181–186View ArticleGoogle Scholar
- Titapoka S (2003) Study on synergism of keratinase produced by Bacillus licheniformis KUB-K0006 and Bacillus pumilus KUB-K0082. Kasetsart University, BangkokGoogle Scholar
- Tiwary E, Gupta R (2012) Rapid conversion of chicken feather to feather meal using dimeric keratinase from Bacillus licheniformis ER-15. J Bioproces Biotech 2:123. doi:10.4172/2155-9821.1000123 View ArticleGoogle Scholar
- Williams CM, Lee CG, Garlich JD, Shih JCH (1991) Evaluation of a bacterial feather fermentation product, feather-lysate, as a feed protein. Poult Sci 70(1):85–94View ArticleGoogle Scholar