Skip to main content

Porphyromonas gingivalis-induced miR-132 regulates TNFα expression in THP-1 derived macrophages



Periodontitis is a chronic inflammatory disease induced by periodontopathogens such as Porphyromonas gingivalis (P. gingivalis). MicroRNAs (miRNAs) are small single-stranded noncoding RNAs that regulate gene expression at the level of translation. MiRNAs have been reported to be involved in inflammatory processes. In this study, we examined the effects of P. gingivalis-induced inflammatory miRNAs expression on TNFα production in THP-1 derived macrophages.


Porphyromonas gingivalis induced the expression of miR-132. P. gingivalis-induced miR-132 expression was significantly inhibited by TLR2/4 knock-down and NF-κB inhibitor. Additionally, miR-132 antagomir strongly repressed production of TNFα. The expression of NFE2L2 and NFAT5, the putative target genes of miR-132 involved in regulation of TNFα, decreased in response to P. gingivalis. Furthermore, miR-132 antagomir rescued P. gingivalis-induced suppression of NFE2L2 and NFAT5.


These results suggest that the induction of miR-132 by P. gingivalis can modulate the pathogenesis of periodontitis induced via regulatory expression of TNFα.


Inflammation is central to effective host defense against invading pathogens. Periodontitis, which is one of the most common human diseases, is an infection-driven chronic inflammatory disease of periodontium and the major cause of tooth loss. Periodontitis is initiated by pathogenic bacteria, such as Porphyromonas gingivalis (P. gingivalis), Tannerella forsythia, and Treponema denticola (Byrne et al. 2009; Socransky and Haffajee 1992). P. gingivalis is a gram negative anaerobic short rod that expresses various virulence factors, including outer membrane vesicles, lipopolysaccharides (LPS), fimbriae, lipoproteins, and gingipains (Pathirana et al. 2000; Zhou and Amar 2006). P. gingivalis modulates the expression of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-8, and TNF-α in human and murine monocytes and macrophages (Agarwal et al. 1995; Park et al. 2014a). The differential regulation of inflammatory cytokines in response to infection is important for recruitment of immune cells in periodontitis tissue and required for orchestration or activation of innate and adaptive immune response.

MicroRNAs (miRNAs) are small single-stranded noncoding RNAs (18–25 nt) that post-transcriptionally regulate gene expression by binding to the 3′ untranslated region (UTR) of target mRNA, leading to mRNA degradation and translational repression (Stoecklin-Wasmer et al. 2012). More than 1000 human miRNAs have been identified in mammals to date (miRBasev.18) (Dai and Ahmed 2011), and they have been found to be associated with diverse biological processes including cell differentiation/proliferation, metabolism, tumorigenesis, and immunity (Ambros 2004; Gregory and Shiekhattar 2005; Taganov et al. 2007).

Recently, several miRNAs related to periodontal inflammation have been reported. In a previous study, we reported differential miRNA expression in healthy and periodontitis tissues (Lee et al. 2011). MiR-132 and miR-146 are inflammatory miRNAs related to bacterial infection. MiR-146 induces a negative feedback mechanism in response to bacterial products-induced TLR signaling to prevent excessive inflammation via suppression of IL-1β, IL-6, IL-8, and TNF-α (Marques-Rocha et al. 2015; Staedel and Darfeuille 2013). Overexpression of miR-132 is sufficient to promote activation of NF-κB and production of IL-8 and MCP-1 (Strum et al. 2009). Although these studies shed some light on the relationship between inflammation and miRNA, bacterial infection-induced miR132 function on inflammatory response is poorly understood. Furthermore, limited data describing the role of miRNAs related to periodontitis or periodontopathogens and the effects of P. gingivalis on the expression of various types of miRNA are available.

In the present study, we investigated whether P. gingivalis can affect the expression of inflammatory miRNAs involved in networks that control innate immunity and extend beyond TLR regulation in THP-1 derived macrophages. We found that P. gingivalis induced miR-132 via TLR2/4 and NF-κB signaling. Moreover, the inhibition of miR-132 expression strongly suppressed the production of TNF-α. The expression of NFE2L2 and NFAT5, potential target molecules of miR-132, decreased in response to P. gingivalis and was recovered by miR-132 antagomir treatment. These results suggest the role of miR-132 in the pathogenesis of periodontitis induced by P. gingivalis.


Bacterial culture

Porphyromonas gingivalis (strain 381) wild type and various mutants (MT10, MT10W, DPG) were grown in GAM broth (Nissui Pharmaceutical, Japan) with 5 mg/ml hemin and 0.5 mg/ml vitamin K under anaerobic conditions at 37 °C. An OD of 1.0 (650 nm) was determined to correlate to 109 CFU/ml. The bacteria were washed and suspended in serum free-RPMI media to infect THP-1 derived macrophages at various multiplicity of infections (MOIs).

Cell lines

THP-1 cells were cultured in RPMI 1640 medium with 10 % heat-inactivated fetal bovine serum (FBS; Gibco), 100 U of penicillin/ml, and 100 μg of streptomycin/ml at 37 °C in a 5 % CO2/95 % air incubator. THP-1 cells were differentiated into macrophage-like cells with 50 ng/ml of Phorbol 12-mystristate 13-acetate (PMA; Sigma).

Real-time quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR)

Total RNA was extracted with TRIzol (Invitrogen, USA) according to the manufacturer’s instructions. For miRNA analysis, 10 ng of RNA from each sample was used for quantitative stem-loop reverse transcription and real-time PCR (qRT-PCR). Quantification of expression of mature miRNAs was performed using a TaqMan micro-RNA RT kit, TaqMan Universal PCR Master Mix, and gene-specific primers and fluorogenic Taqman probes (Applied Biosystems, USA). MiRNA expression values were calculated using human RNU44 as an endogenous reference. For miRNA target gene analysis, the reverse transcription of total RNA to cDNA was performed using AccuPower RT PreMix (Bioneer Co, South Korea). The mRNA expression levels were quantified by real-time PCR using a Light Cycler instrument (Roche Applied Science, Mannheim, Germany) with SYBR Green PCR Master Mix (Qiagen, USA) according to the manufacturer’s instructions.

Transient transfection

For RNA interference assay, human siRNAs for TLR2, TLR4, and non-targeting control oligonucleotides were obtained from Qiagen. The siRNA oligonucleotides in this pool were as follows: TLR2, TLR4, and non-targeting control siRNA oligonucleotides. For siRNA experiments in THP-1 cells, cells were seeded in 6-well plates at a density of 1 × 106 cells per well in the presence of 50 nM PMA. The differentiated cells were then transfected with siRNA oligonucleotides (1200 ng) for 48 h using Attractene Transfection Reagent (Qiagen, USA) in 1 ml of RPMI. MiR-132 functional analyses were performed by transfecting synthetic antagomir (20 pmol) in THP-1 cells using Attractene Transfection Reagent.

NF-κB DNA binding assay

Binding of p65 to the double-stranded NF-κB oligonucleotide was measured using a NF-κB Combo Transcription Factor Assay kit (Cayman Chemical Company, USA).

Measurement of TNF-α and IL-1β

To determine the amount of TNF-α and IL-1β released into the culture media after P. gingivalis stimulation, we analyzed the amount in accordance with the manufacturer’s instructions using an ELISA kit (eBioscience, San Diego, CA, USA).

Immunoblot analysis

Cells were washed in ice-cold PBS and lysed, after which a total of 50 μg proteins were separated by 15 % SDS-PAGE, transferred to a PDVF membrane and probed with the indicated antibodies. Reactivity was determined using HRP-conjugated anti-mouse, anti-rabbit or anti-mouse IgG secondary antibody (Jackson Immuno Research Laboratories, USA) (diluted 1:5000) and the signals were visualized using Super Signal West Femto maximum sensitivity substrate (Pierce, USA) with a LAS-4000 FUJIFILM Luminescent Image Analyzer (Fujifilm, Japan).

ASC pyroptosome quantitation in live cells

THP-1/ASC-GFP cells were seeded in 8 well chambers and then primed with PMA (50 nM). THP-1/ASC-GFP cells were transfected with miR-132 antagomir and further challenged with P. gingivalis. ASC pyroptosome formation was observed by confocal Laser-Scanning Microscopy (Carl Zeiss, USA) at various time points. At least 400 cells were counted to enumerate the number of cells containing the ASC-GFP pyroptosome at the end of each period. The percentage of cells with ASC pyroptosome was calculated by dividing the number of cells with ASC pyroptosome by the total cells counted.


Statistically significant differences between samples were analyzed with an unpaired, one-tailed Student’s t test. The data are shown as the mean ± standard deviation (SD).


Porphyromonas gingivalis infection up-regulates miR-132 expression in THP-1 derived macrophages

To investigate the relationship between periodontitis and miRNA, we examined the effects of P. gingivalis on the expression of inflammatory miRNAs, miR-132 and miR-146 in THP-1 derived macrophages. MiR-132 and miR-146 expression were induced by live P. gingivalis infection in a MOI-dependent and time-dependent manner (Fig. 1a, b). To gain more insight into the mechanism by which P. gingivalis induces miRNAs expression, the mutant strains tested included MT10 (RgpA-), MT10W (RpgA-, Kgp-) and DPG (fimbriae-). The mutant strains showed less of an increase of expression of miR-132 and miR-146 than wild-type strain (Fig. 1c, d). These results suggest that the virulence factors, gingipain and fimbriae, may be involved in induction of miR-132 to regulate expression of TNFα. We focused on miR-132 for further investigation because the relationship between miR-132 and P. gingivalis has not been studied, even though miR-132 is associated with regulation of inflammation.

Fig. 1
figure 1

MiR-132 and miR-146 were induced by live P. gingivalis infection. THP-1-derived macrophages were infected with live P. gingivalis (MOI 50, 100, 1000) for the indicated times. Total RNA was purified from the respective cell pellets and analyzed by qRT-PCR for the expression of miR-132 (a) and miR-146 (b). THP-1 derived macrophages were infected with various strains of P. gingivalis (MOI 100) for the indicated times (c, d). MOI multiplicity of infection

Porphyromonas gingivalis induces miR-132 expression through TLR2/4 signaling and NF-κB signaling in THP-1 derived macrophages

Because P. gingivalis activates both TLR2 and TLR4, we investigated the involvement of TLR2 and TLR4 in P. gingivalis-induced miRNA expression by RNA interference assay. Knockdown of TLR2 and TLR4 significantly decreased P. gingivalis-induced miR-132 expression relative to siRNA control (Fig. 2a). TLR2 and/or TLR4 activate NF-κB for the induction of inflammation. Thus, we examined the involvement of NF-κB in P. gingivalis-induced miR-132 expression. The activation of NF-κB, as measured by p65-50 binding to an NF-κB consensus oligonucleotide, was observed at 3 h post-infection (Fig. 2b). The induction of miR-132 expression by P. gingivalis was inhibited by pretreatment of the cells with PDTC, an NF-κB inhibitor (Fig. 2c). These results suggest that P. gingivalis induces miR-132 through the activation of TLR2/4 and NF-κB.

Fig. 2
figure 2

TLR2/4 and NF-κB is required for P. gingivalis-induced miR-132 expression. a THP-1 cells were transfected with 100 nM siTLR2 and siTLR4 for 48 h, then infected with P. gingivalis (MOI 100) for 6 h. b THP-1 derived macrophages were infected with P. gingivalis (MOI 100) for the indicated times. Nuclear extracts were analyzed for p65 and p50 binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA Assay kit. c THP-1 derived macrophages were pretreated with 300 μM PDTC for 1 h, then infected with live P. gingivalis (MOI 100) for indicated times, after which the total RNA was purified from the respective cell pellets and analyzed by qRT-PCR for the expression of miR-132. The data represent mean values ± SD (n 3). Bar graphs show the fold increase of protein expression compared with control cells; columns show mean values of n 3 samples; error bars show standard deviation

The inhibition of miR-132 expression down-regulates the expression of TNFα, but not IL-1β, induced by P. gingivalis infection in THP-1 derived macrophages

To test the effects of miR-132 on pro-inflammatory cytokine production by live P. gingivalis infection, THP-1 cells were transfected with miR-132 antagomir. As shown in Fig. 3a, miR-132 expression in miR-132 antagomir transfected cells showed a 90 % reduction compared with mock transfected cells. MiR-132 antagomir treatment (transfection) resulted in a decrease in P. gingivalis–induced TNFα (Fig. 3b, c), but not IL-1β secretion (Fig. 3d). Because the production of mature IL-1β required the activation of inflammasome, we investigated the effects of miR-132 on inflammasome activation. As shown in Fig. 3e, miR-132 antagomir transfection did not affect the formation of ASC pyroptosome in response to P. gingivalis infection. These results indicate that P. gingivalis induces miR-132 expression and subsequently enhances the expression of TNFα.

Fig. 3
figure 3

MiR-132 is involved in TNF-α, but not IL-1β, induced by P. gingivalis infection in THP-1 derived macrophages. THP cells were transfected with control or miR-132 antagomir (20 pmol). At 24 h post-transfection, RNA was extracted and miR-132 expression was verified by qRT-PCR (a). At 24 h post-transfection, THP-1 cells were infected with live P. gingivalis (MOI 100) for 6 h (b) or 24 h (c, d). Cell culture supernatants were assayed for human TNF-α (b, c) and IL-1β (d) using ELISA. e PMA-primed ASC-GFP stable expressing-THP-1 cells were transfected with control or miR-132 antagomir (20 pmol). At 24 h post-transfection, samples were infected with P. gingivalis for 24 h and ASC-pyroptosomes were then observed and photographed by fluorescence confocal microscopy (right panel). The graph on the left shows the percentages of cells containing the ASC pyroptosome. The percentages of cells containing the ASC pyroptosome were calculated as described in the “Methods” section. TNFα tumor necrosis factor. The data represent mean values ± SD (n 3). Bar graphs show the fold increase of protein expression compared with control cells; columns show mean values of n 3 samples; error bars show standard deviation

Inhibition of miR-132 expression restored the expression of NFE2L2 and NFAT5 decreased by P. gingivalis infection in THP-1 derived macrophages

To identify potential targets of miR-132 involved in inflammatory response, we used the online bioinformatics program TargetScan ( NFE2L2 and NFAT5 were identified as predicted targets of miR-132. To test whether miR132 would modulate NFE2L2 and NFAT5 as well as inflammatory cytokines in P. gingivalis-infected THP-1 derived macrophages, THP-1 cells were transfected with miR-132 antagomir. As shown Fig. 4a and b, P. gingivalis decreased NFE2L2 and NFAT5 expression and miR-132 antagomir up-regulated NFE2L2 and NFAT5 expression in P. gingivalis-infected THP-1 derived macrophages. These data suggest the possible direct repression of NFE2L2 and NFAT5 by miR-132.

Fig. 4
figure 4

MiR-132 regulates the expression of NFE2L2 and NFAT5 induced by P. gingivalis infection in THP-1 cells. a, b THP cells were transfected with control or miR-132 antagomir (50 pmol). At 24 h post-transfection, THP-1 cells were differentiated by PMA for 24 h, RNA was extracted and the expression of NFE2L2 and NFAT5 was verified by qRT-PCR. The data represent mean values ± SD (n 3). Bar graphs show the fold increase of protein expression compared with control cells; columns show mean values of n 3 samples; error bars show standard deviation. c A proposed model of P. gingivalis-induced miR-132 regulates TNFα in THP-1-derived macrophages


In this study, we investigated the effects of P. gingivalis on the expression of inflammatory miRNAs, miR-132 and miR-146, and the role of miR-132 in live P. gingivalis-challenged THP-1-derived macrophages. P. gingivalis induced miR-132 expression via TLR2/4 and NF-κB signaling, and the enhanced miR-132 expression was closely associated with the increased production of TNFα (Fig. 4c).

MiRNAs are abundant in the human genome and control various important cellular processes, including proliferation, differentiation, metabolism, apoptosis and inflammation (Ambros 2004; Zhang and Su 2009). A single miRNA is loaded into the RNA-induced silencing complex (RISC) and guides this complex to the 3′ UTRs of target mRNA, leading to suppressed target protein expression. Several lines of evidence suggest that miRNAs play critical roles in a number of chronic inflammatory diseases (Plank et al. 2013; Zhang et al. 2014). In innate immune response, specific miRNAs can be up- or down-regulated by inflammatory stimuli. The miRNAs significantly linked to inflammatory response were miR-146, miR-155, miR-21, miR-9 and miR-132 (Dai and Ahmed 2011; Schetter et al. 2010), among which miR-132, miR-146, and miR-155 are inflammatory miRNAs. miR-146 and miR-155, but not miR132, are known to be associated with periodontal disease (Xie et al. 2011). MiR-146 expression increased in periodontal-disease gingival tissue (Lee et al. 2011; Xie et al. 2011) and P. gingivalis-LPS induced miR-146 expression in THP-1 cells/THP-1-derived macrophages (Honda et al. 2012) and human gingival fibroblasts (Xie et al. 2013). Honda et al. (2012) suggested that P. gingivalis-LPS-induced miR-146 expression did not modulate proinflammatory cytokines production. Consistent with the above reports, we found that P. gingivalis induced miR-146 in THP-1-derived macrophages. Although these studies demonstrated the involvement of miRNA in periodontitis, the association of miR-132 with periodontitis has not yet been reported.

MiR-132 is well characterized in brain neurons and plays an important role in inflammation, angiogenesis, and neural development (Magill et al. 2010; Mulik et al. 2012; Shaked et al. 2009). MiR-132 modulates neuronal survival in AD neurodegeneration (Wong et al. 2013) and attenuates inflammation in the mouse brain by targeting acetylcholinesterase (Shaked et al. 2009). In innate immune response, miR-132 plays important roles in TLR2 ligand–induced tolerance and cross-tolerance by targeting IRAK4 (Nahid et al. 2013). Nevertheless, the function of miR-132 has been described in only a few studies and is still poorly understood in innate immune response. Our data are the first to reveal the relationship between miR-132 and P. gingivalis-mediated inflammatory response in THP-1-derived macrophages. The bacterial cell surface components facilitate growth, nutrient acquisition, colonization, biofilm formation and evasion of host defense (Yoshimura et al. 2009). Several reports suggest that gingipains is critical for proinflammatory response in periodontal disease progression (Kataoka et al. 2014; Tancharoen et al. 2015). Fimbriae are potent stimulators of inflammation (Takahashi et al. 2006), and P. gingivalis fimbriae significantly induced TNFα production via TLR2 in macrophages (Aoki et al. 2010). Interestingly, our data showed that gingipain mutants (MT10 and MT10 W) and fimbriae mutant (DPG) of P. gingivalis resulted in less increase of miR-132 compared to wild-type. Even though involvement of gingipain and fimbriae in miR132 expression has not yet been investigated, these reports coupled with our results support the possibility that gingipain and fimbriae contribute to production of TNFα via miR-132 induction.

In our study, live P. gingivalis induced miR-132, and its regulation was dependent on TLRs. TLRs recognize pathogens and activate signaling pathways including immune and inflammatory response to regulate cytokines, chemokines, and adhesion molecules and eliminate pathogens. Several reports recently revealed that TLR signaling modulates various miRNAs (Nahid et al. 2011; O’Neill et al. 2011). miR-146, miR-155 and miR-21 have been central in many miRNA studies due to their expression levels following TLR activation (Quinn and O’Neill 2011). Furthermore, miR-9, miR-147, miR-27b, miR-223, and let-7e are induced by TLR stimulus or pathogen infection (He et al. 2014). However, only a few studies have investigated TLR-mediated miR-146 expression in periodontal disease (Honda et al. 2012; Xie et al. 2013). Indeed, this study is the first study to suggest P. gingivalis induces miR-132 via TLR signaling.

The expression of most TLR-responsive miRNAs described to date depends on NF-κB activity. MiR-155 positively regulates inflammation through the TLR-NF-κB pathway in response to Francisella (Cremer et al. 2009). MiR-9, miR-146, miR-181b and miR-21 are also directly regulated through the NF-κB-dependent pathway (Ma et al. 2011). Conversely, miR-29b, let-7i, miR-98, miR-107, miR-27a, and miR-532-5p are negatively regulated by the TLR-NF-κB pathway (He et al. 2014). We found that inhibition of TLR signaling or NF-κB abolished P. gingivalis-induced miR-132 expression. Although this suggests that miR-132 may be directly regulated by the TLR2/4-NF-κB signaling pathway, further studies are needed to better understand the exact mechanism.

Cytokines are key modulators of inflammatory response classified as proinflammatory (IL-1, IL-6, IL15 and TNFα) or anti-inflammatory (IL-4, IL-10, TFGβ and IFNα) (Schetter et al. 2010). Among various cytokines, TNFα affects many functions such as inflammation, tumor promotion, and anti-microbial immunity (Sedger and McDermott 2014). The expression of TNFα was higher in periodontitis patients than healthy controls, and periodontal bacteria stimulate expression of TNFα (Preshaw and Taylor 2011). Consistent with the above data, we previously reported that TNFα was higher in the GCF of periodontitis patients and P. gingivalis-LPS induced gene expression and protein synthesis of TNFα (Han et al. 2005; Park et al. 2014a). Similar to TNFα, IL-1β is a pro-inflammatory cytokine critical to inflammatory response against pathogens that is well known for involvement in periodontal disease. We recently found that the levels of IL-1β and inflammasome components increased in periodontitis patients and P. gingivalis induced IL-1β release via TLR2/TLR4-NLRP3/AIM2 inflammasome-caspase-1 pathway activation (Park et al. 2014b). In the current study, we found that miR-132 positively regulates TNFα, but not IL-1β. Furthermore, miR-132 did not affect inflammasome activation. However, some studies suggest that IL-1β is a target of miR-132, in contrast to our results (Fen et al. 2014; Hanieh and Alzahrani 2013). The precise reasons for this difference are not clear, but these results may be dependent on cell type and stimuli; accordingly, these mechanisms should be further investigated.

Nuclear factor E2-related factor 2 (NFE2L2 or Nrf2) is a master transcription factor essential for protection from oxidative stress via modulation of ARE-dependent genes, including hemo oxygenase-1 (HO-1), a well-known anti-inflammatory enzyme (Hu et al. 2010; Kim et al. 2010). Park et al. recently reported that the anti-inflammatory effect of Schisandrin on P. gingivalis-LPS-stimulated macrophages was due to induction of HO-1 and activation of Nrf-2. Inhibition of miR-132 restored the ochartoxin-driven decrease of Nrf2 and HO-1 expression as well as the increase in ROS production (Stachurska et al. 2013). Our study also found that inhibition of miR-132 rescued P. gingivalis-mediated deduction of Nrf-2 expression. Moreover, our results indicated that P. gingivalis-induced production of TNFα may be enhanced by miR-132 via miR-132-mediated suppression of Nrf2 expression and anti-inflammatory effect of Nrf2. Nuclear factor of activated T cells 5 (NFAT5) is a transcription factor involved in immune response by regulating TNFα expression via direct binding to TNF promoter and enhancing TNF transcription (Esensten et al. 2005). Although NFAT5 positively regulates TNFα, our results showed that P. gingivalis and P. gingivalis-induced miR-132 negatively regulated NFAT5 expression. We assume that these results occurred due to a negative-feedback loop in which P. gingivalis-induced miR-132 attenuated pro-inflammatory effects of P. gingivalis by targeting NFAT5 expression. Since we do not yet fully understand how miR-132-targeted NFAT5 affects the expression of TNFα by P. gingivalis, the exact involvement of miR-132 and NFAT5 in P. gingivalis-induced inflammation will be confirmed later.


In this study, live P. gingivalis infection induced miR-132 via TLR signaling and activation of NF-κB. Furthermore, inhibition of miR-132 expression strongly repressed the production of TNFα and increased NFE2L2 and NFAT5. This is the first study to describe the novel role of miR-132 in the pathogenesis of P. gingivalis. These results support the concept that miR-132 modulates TNFα via inhibition of its target genes, which may provide a new window of opportunity to investigate therapeutic intervention for P. gingivalis-induced TNFα associated diseases such as periodontitis.


  • Agarwal S, Piesco N, Johns L, Riccelli A (1995) Differential expression of IL-1 beta, TNF-alpha, IL-6, and IL-8 in human monocytes in response to lipopolysaccharides from different microbes. J Dent Res 74:1057–1065

    Article  Google Scholar 

  • Ambros V (2004) The functions of animal microRNAs. Nature 431:350–355

    Article  Google Scholar 

  • Aoki Y, Tabeta K, Murakami Y, Yoshimura F, Yamazaki K (2010) Analysis of immunostimulatory activity of Porphyromonas gingivalis fimbriae conferred by Toll-like receptor 2. Biochem Biophys Res Commun 398:86–91

    Article  Google Scholar 

  • Byrne S, Dashper S, Darby I, Adams G, Hoffmann B, Reynolds E (2009) Progression of chronic periodontitis can be predicted by the levels of Porphyromonas gingivalis and Treponema denticola in subgingival plaque. Oral Microbiol Immunol 24:469–477

    Article  Google Scholar 

  • Cremer T, Ravneberg D, Clay C, Piper-Hunter M, Marsh C, Elton T, Gunn J, Amer A, Kanneganti T, Schlesinger L et al (2009) MiR-155 induction by F. novicida but not the virulent F. tularensis results in SHIP down-regulation and enhanced pro-inflammatory cytokine response. PLoS ONE 4:e8508

    Article  Google Scholar 

  • Dai R, Ahmed S (2011) MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases. Transl Res 157:163–179

    Article  Google Scholar 

  • Esensten J, Tsytsykova A, Lopez-Rodriguez C, Ligeiro F, Rao A, Goldfeld A (2005) NFAT5 binds to the TNF promoter distinctly from NFATp, c, 3 and 4, and activates TNF transcription during hypertonic stress alone. Nucleic Acids Res 33:3845–3854

    Article  Google Scholar 

  • Fen L, Rong J, Zhenguo Z, Ning Z, Liang X, Cheng N, Kejian Q (2014) The expression changes in microRNA-132 in the lipopolysaccharide-induced inflammation of rat alveolar macrophages. Zhonghua Wei Zhong Bing Ji Jiu Yi Xu 26:80–83

    Google Scholar 

  • Gregory R, Shiekhattar R (2005) MicroRNA biogenesis and cancer. Cancer Res 65:3509–3512

    Article  Google Scholar 

  • Han S, Jeong S, Nam Y, Yang K, Lim H, Chung J (2005) Xylitol inhibits inflammatory cytokine expression induced by lipopolysaccharide from Porphyromonas gingivalis. Clin Diagn Lab Immunol 12:1285–1291

    Google Scholar 

  • Hanieh H, Alzahrani A (2013) MicroRNA-132 suppresses autoimmune encephalomyelitis by inducing cholinergic anti-inflammation: a new Ahr-based exploration. Eur J Immunol 43:2771–2782

    Article  Google Scholar 

  • He X, Jing Z, Cheng G (2014) MicroRNAs: new regulators of Toll-like receptor signalling pathways. Biomed Res Int 2014:1–14

    Google Scholar 

  • Honda T, Takahashi N, Miyauchi S, Yamazaki K (2012) Porphyromonas gingivalis lipopolysaccharide induces miR-146a without altering the production of inflammatory cytokines. Biochem Biophys Res Commun 420:918–925

    Article  Google Scholar 

  • Hu R, Saw C, Yu R, Kong A (2010) Regulation of NF-E2-related factor 2 signaling for cancer chemoprevention: antioxidant coupled with antiinflammatory. Antioxid Redox Signal 13:1679–1698

    Article  Google Scholar 

  • Kataoka S, Baba A, Suda Y, Takii R, Hashimoto M, Kawakubo T, Asao T, Kadowaki T, Yamamoto K (2014) A novel, potent dual inhibitor of Arg-gingipains and Lys-gingipain as a promising agent for periodontal disease therapy. FASEB J. 28:3564–3578

    Article  Google Scholar 

  • Kim J, Cha Y, Surh Y (2010) A protective role of nuclear factor-erythroid 2-related factor-2 (Nrf2) in inflammatory disorders. Mutat Res 690:12–23

    Article  Google Scholar 

  • Lee Y, Na H, Jeong S, Jeong S, Park H, Chung J (2011) Comparison of inflammatory microRNA expression in healthy and periodontitis tissues. Biocell 35:43–49

    Google Scholar 

  • Ma X, Becker Buscaglia L, Barker J, Li Y (2011) MicroRNAs in NF-kappaB signaling. J Mol Cell Biol 3:159–166

    Article  Google Scholar 

  • Magill S, Cambronne X, Luikart B, Lioy D, Leighton B, Westbrook G, Mandel G, Goodman R (2010) microRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampus. Proc Natl Acad Sci USA 107:20382–20387

    Article  Google Scholar 

  • Marques-Rocha J, Samblas M, Milagro F, Bressan J, Martínez J, Marti A (2015) Noncoding RNAs, cytokines, and inflammation-related diseases. FASEB J 29:3595–3611

    Article  Google Scholar 

  • Mulik S, Xu J, Reddy P, Mulik S, Xu J, Reddy P, Rajasaqi N, Gimenez F, Sharma S, Lu P et al (2012) Role of miR-132 in angiogenesis after ocular infection with herpes simplex virus. Am J Pathol 171:525–534

    Article  Google Scholar 

  • Nahid M, Satoh M, Chan E (2011) MicroRNA in TLR signaling and endotoxin tolerance. Cell Mol Immunol 8:388–403

    Article  Google Scholar 

  • Nahid M, Yao B, Dominguez-Gutierrez P, Kesavalu L, Satoh M, Chan E (2013) Regulation of TLR2-mediated tolerance and cross-tolerance through IRAK4 modulation by miR-132 and miR-212. J Immunol. 190:1250–1263

    Article  Google Scholar 

  • O’Neill L, Sheedy F, McCoy C (2011) MicroRNAs: the fine-tuners of Toll-like receptor signalling. Nat Rev Immunol 11:163–175

    Article  Google Scholar 

  • Park E, Na H, Kim S, Wallet S, Cha S, Chung J (2014a) Xylitol, an anticaries agent, exhibits potent inhibition of inflammatory responses in human THP-1-derived macrophages infected with Porphyromonas gingivalis. J Periodontol 85:212–223

    Article  Google Scholar 

  • Park E, Na H, Song Y, Shin S, Kim Y, Chung J (2014b) Activation of NLRP3 and AIM2 inflammasomes by Porphyromonas gingivalis infection. Infect Immun 82:112–123

    Article  Google Scholar 

  • Pathirana R, O’Brien-Simpson N, Reynolds E (2000) Host immune responses to Porphyromonas gingivalis antigens. Periodontology 52:218–237

    Article  Google Scholar 

  • Plank M, Maltby S, Mattes J, Foster P (2013) Targeting translational control as a novel way to treat inflammatory disease: the emerging role of microRNAs. Clin Exp Allergy 43:981–999

    Article  Google Scholar 

  • Preshaw P, Taylor J (2011) How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol 38:60–84

    Article  Google Scholar 

  • Quinn S, O’Neill L (2011) A trio of microRNAs that control Toll-like receptor signalling. Int Immunol 23:421–425

    Article  Google Scholar 

  • Schetter A, Heegaard N, Harris C (2010) Inflammation and cancer: interweaving microRNA, free radical, cytokine and p53 pathways. Carcinogenesis 31:37–49

    Article  Google Scholar 

  • Sedger L, McDermott M (2014) TNF and TNF-receptors: from mediators of cell death and inflammation to therapeutic giants—past, present and future. Cytokine Growth Factor Rev 25:453–472

    Article  Google Scholar 

  • Shaked I, Meerson A, Wolf Y, Avni R, Greenberg D, Gilboa-Geffen A, Soreq H (2009) MicroRNA-132 potentiates cholinergic anti-inflammatory signalling by targeting acetylcholinesterase. Immunity 31:965–973

    Article  Google Scholar 

  • Socransky S, Haffajee A (1992) The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol 63:322–331

    Article  Google Scholar 

  • Stachurska A, Ciesla M, Kozakowska M, Wolffram S, Boesch-Saadatmandi C, Rimbach G, Jozkowicz A, Dulak J, Loboda A (2013) Cross-talk between microRNAs, nuclear factor E2-related factor 2, and heme oxygenase-1 in ochratoxin A-induced toxic effects in renal proximal tubular epithelial cells. Mol Nutr Food Res 57:504–551

    Article  Google Scholar 

  • Staedel C, Darfeuille F (2013) MicroRNAs and bacterial infection. Cell Microbiol 15:1496–1507

    Article  Google Scholar 

  • Stoecklin-Wasmer C, Guarnieri P, Celenti R, Demmer R, Kebschull M, Papapanou P (2012) MicroRNAs and their target genes in gingival tissues. J Dent Res 91:934–940

    Article  Google Scholar 

  • Strum J, Johnson J, Ward J, Xie H, Feild J, Hester A, Alford A, Waters K (2009) MicroRNA 132 regulates nutritional stress-induced chemokine production through repression of SirT1. Mol Endocrinol 23:1876–1884

    Article  Google Scholar 

  • Taganov K, Boldin M, Baltimore D (2007) MicroRNAs and immunity: tiny players in a big field. Immunity 26:133–137

    Article  Google Scholar 

  • Takahashi Y, Davey M, Yumoto H, Gibson F, Genco C (2006) Fimbria-dependent activation of pro-inflammatory molecules in Porphyromonas gingivalis infected human aortic endothelial cells. Cell Microbiol 8:738–757

    Article  Google Scholar 

  • Tancharoen S, Matsuyama T, Kawahara K, Tanaka K, Lee L, Machigashira M, Noguchi K, Ito T, Imamura T, Potempa J et al (2015) Cleavage of host cytokeratin-6 by lysine-specific gingipain induces gingival inflammation in periodontitis patients. PLoS ONE 10:e0117775

    Article  Google Scholar 

  • Wong H, Veremeyko T, Patel N, Lemere C, Walsh D, Esau C, Vanderburg C, Krichevsky A (2013) De-repression of FOXO3a death axis by microRNA-132 and -212 causes neuronal apoptosis in Alzheimer’s disease. Hum Mol Genet 22:3077–3092

    Article  Google Scholar 

  • Xie Y, Shu R, Jiang S, Liu D, Zhang X (2011) Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues. Int J Oral Sci. 3:125–134

    Article  Google Scholar 

  • Xie Y, Shu R, Jiang S, Liu D, Ni J, Zhang X (2013) MicroRNA-146 inhibits pro-inflammatory cytokine secretion through IL-1 receptor-associated kinase 1 in human gingival fibroblasts. J Inflamm 10:1–9

    Article  Google Scholar 

  • Yoshimura F, Murakami Y, Nishikawa K, Hasegawa Y, Kawaminami S (2009) Surface components of Porphyromonas gingivalis. J Periodontal Res 44:1–12

    Article  Google Scholar 

  • Zhang R, Su B (2009) Small but influential: the role of microRNAs on gene regulatory network and 3′UTR evolution. J Genet Genomics 36:1–6

    Article  Google Scholar 

  • Zhang X, Zhang Y, Liu Z (2014) MicroRNA in chronic rhinosinusitis and allergic rhinitis. Curr Allergy Asthma Rep 14:415

    Article  Google Scholar 

  • Zhou Q, Amar S (2006) Identification of proteins differentially expressed in human monocytes exposed to Porphyromonas gingivalis and its purified components by high-throughput immunoblotting. Infect Immun 74:1204–1214

    Article  Google Scholar 

Download references

Authors’ contributions

The work presented here was carried out in collaboration among all authors. PMH, PEJ, NSH and CJ designed the research. PMH and PEJ analyzed the data, interpreted the results. PMH drafted the manuscript, which was proofread by KHJ, NHS and CJ. All authors read and approved the final manuscript.


This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (NRF-2011-0012897).

Competing interests

The authors declare that they have no competing interests.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Jin Chung.

Additional information

Mi Hee Park and Eunjoo Park contributed equally to this work

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Park, M.H., Park, E., Kim, HJ. et al. Porphyromonas gingivalis-induced miR-132 regulates TNFα expression in THP-1 derived macrophages. SpringerPlus 5, 761 (2016).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: