Fig. 4

MiR-132 regulates the expression of NFE2L2 and NFAT5 induced by P. gingivalis infection in THP-1 cells. a, b THP cells were transfected with control or miR-132 antagomir (50 pmol). At 24 h post-transfection, THP-1 cells were differentiated by PMA for 24 h, RNA was extracted and the expression of NFE2L2 and NFAT5 was verified by qRT-PCR. The data represent mean values ± SD (n ≧ 3). Bar graphs show the fold increase of protein expression compared with control cells; columns show mean values of n ≧ 3 samples; error bars show standard deviation. c A proposed model of P. gingivalis-induced miR-132 regulates TNFα in THP-1-derived macrophages