Fig. 2

TLR2/4 and NF-κB is required for P. gingivalis-induced miR-132 expression. a THP-1 cells were transfected with 100 nM siTLR2 and siTLR4 for 48 h, then infected with P. gingivalis (MOI 100) for 6 h. b THP-1 derived macrophages were infected with P. gingivalis (MOI 100) for the indicated times. Nuclear extracts were analyzed for p65 and p50 binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA Assay kit. c THP-1 derived macrophages were pretreated with 300 μM PDTC for 1 h, then infected with live P. gingivalis (MOI 100) for indicated times, after which the total RNA was purified from the respective cell pellets and analyzed by qRT-PCR for the expression of miR-132. The data represent mean values ± SD (n ≧ 3). Bar graphs show the fold increase of protein expression compared with control cells; columns show mean values of n ≧ 3 samples; error bars show standard deviation