Fabrication and vibration characterization of curcumin extracted from turmeric (Curcuma longa) rhizomes of the northern Vietnam
- Hoang Van Nong†1,
- Le Xuan Hung†2,
- Pham Nam Thang†1,
- Vu Duc Chinh†1,
- Le Van Vu†3,
- Phan Tien Dung†4, 1,
- Tran Van Trung†5 and
- Pham Thu Nga†1, 2Email authorView ORCID ID profile
© The Author(s) 2016
Received: 15 November 2015
Accepted: 12 July 2016
Published: 22 July 2016
In this report, we present the research results on using the conventional method and microwave technology to extract curcuminoid from turmeric roots originated in different regions of Northern Vietnam. This method is simple, yet economical, non-toxic and still able to achieve high extraction performance to get curcuminoid from turmeric roots. The detailed results on the Raman vibration spectra combined with X-ray powder diffraction and high-performance liquid chromatography/mass spectrometry allowed the evaluation of each batch of curcumin crystalline powder sample received, under the conditions of applied fabrication technology. Also, the absorption and fluorescence spectroscopies of the samples are presented in the paper. The information to be presented in this paper: absorption and fluorescence spectroscopies of the samples; new experimental study results on applied technology to mass-produce curcumin from turmeric rhizomes; comparative study results between fabricated samples and marketing curcumin products—to state the complexity of co-existing crystalline phase in curcumin powder samples. We noticed that, it is possible to use the vibration line at ~959 cm−1—characteristic of the ν C=O vibration, and the ~1625 cm−1 line—characteristic of the ν C=O and ν C=C vibration in curcumin molecules, for preliminary quality assessment of naturally originated curcumin crystalline powder samples. Data on these new optical spectra will contribute to the bringing of detailed information on natural curcumin in Vietnam, serving research purposes and applications of natural curcumin powder and nanocurcumin in Vietnam, as well as being initial materials for the pharmaceutical, cosmetics or functional food industries.
Curcumin is a natural, yellow colored phenolic antioxidant and was first extracted in an impure form by Vogel et al. (1815). Curcumin is a hydrophobic phenol having the chemical name [1,7-bis (4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (Kolev et al. 2005) and empirical formula C21H20O6. Turmeric does not contain only curcumin (compound I), but also its analogues demetoxycurcumin (compound II), bisdemetoxycurcumin (compound III) along with the water-soluble protein turmerin, and curcumin is responsible for its characteristic yellow-to-bright-orange color. The biological and molecular properties of curcumin and its analogues are similar, thus it is suggested that in natural pathways the bisdemethoxycurcumin converts to demethoxycucumin, which in turn converts to curcumin.
Curcumin is known for its wide-ranging pharmacological applications such as antioxidant, anti-inflammatory, antimicrobial, antimalarial, anti-carcinogenic, anti-HIV agent, etc. (Sanphui et al. 2011; Agarwal and Sung 2009). Curcumin, being a diphenolic compound extracted from the rhizome of turmeric, is a prominent candidate for treating cystic fibrosis, Alzheimer’s and malarial diseases in addition to cancer (Maheshwari et al. 2006; Yallapu et al. 2012). Curcumin is safe even at a high dose of 12 g per day (Qureshi et al. 1992; Lao et al. 2006) proven by experiments on both animals and humans. The first crystal structure of curcumin was reported in 1982 in the monoclinic space group P2/n (Tønnesen et al. 1982).
In Vietnam, detailed studies on the crystalline phase and optical spectra of curcumin powder extracted from yellow turmeric are yet to be announced. In this study, we present the research results on natural curcuminoid samples, extracted from turmeric rhizomes grown in Northern Vietnam using the microwave technology, and compare it with the conventional method. The primary purpose of this research is to analyze the technology to produce curcumin, the initial material for producing nanocurcumin, from Vietnamese turmeric rhizomes, and apply it in the pharmaceutical industry. Therefore, we have conducted comparative studies on the crystalline phase of natural curcumin crystal powder, between being fabricated using the conventional method and microwave technology, then present the absorption and fluorescence spectroscopy in this report. This is the first detailed study, carried out on curcumin extracted from Vietnamese turmeric rhizomes with the production scale of hundreds of kilograms of turmeric. The analyzed curcumin content by the high performance liquid chromatography/mass spectrometry method (HPLC/MS) on the three components of curcuminoid was presented to clarify the studies on crystalline phase structure of these natural powder samples.
Fresh rhizomes of Curcuma longa are purchased from local markets at Khoai Chau, Hung Yen and Hai Duong. After collection, fresh rhizomes were immediately kept in shed, washed with normal water, then again with distilled water. For the next step, they were peeled, shredded into small threads and then ready for curcumin extraction. Different solvents used were absolute ethanol, hexane and isopropanol, all with the pure grade (P.A.), purchased from Xilong Chemical Co. Ltd. (China).
Curcumin extracting methods
We used two methods: (1) conventional solvent-extraction method with eco-friendly absolute ethanol, and (2) microwave assisted extraction. These methods were mentioned early in Priyadarsini (2014), Paulucci et al. (2013), Lee et al. (2012a, b), Li et al. (2014), Patel et al. (2000), Kim et al. (2013).
Conventional solvent-extraction method
Fiber-formed rhizome (1 kg) is kept in a 3 L amber glass beaker and extracted at room temperature with 1 L of absolute ethanol, soaked and stirred (400 rounds/min). Cover the beaker to prevent loss of ethanol, then leave it for a week. The extracts were filtered separately under vacuum. They can be removed from the ethanol and stored for use as a dry composition. The prepared ethanol extract is transferred to the round-bottomed flask of a rotary evaporator and heated to a maximum temperature of 45 °C. The condensed ethanol is collected via a condenser for the purpose of re-using in subsequent extractions, and the rest of the extracted solution that is free from solvent is removed from the flask for curcumin precipitation with hexane. The resultant powder is stored in an amber bottle until needed. The dried powder was weighed accurately and percentage yield was calculated (sample N1). Afterwards, weigh out a small amount of this sample and prepared it into a solution with concentration 1 mg/mL for HPLC/MS analysis.
Microwave assisted extraction
An extraction system comprised of a domestic microwave oven manufactured by SANIO Electric Co. Ltd. equipped with a magnetron of 2450 MHz with a nominal maximum power of 1000 W, 10 power levels, timed controller, convection temperature sensors and exhaust system is used for microwave extraction. The microwave-assisted extraction is carried out as following: place 1 kg of the fresh curcuma threads in a glass vessel and irradiated for a pre-defined time period (1, 3, 5 min) at 200 W microwave power. After irradiation, pour the absolute ethanol into the curcuma longthreads (mass: solvent ratio 1:1 w/v). The ethanol-soaked curcuma longthreads are irradiated at 800 W microwave power for 2, 4 and 6 min. The extraction is performed at 700 W microwave power for 5 min. After the defined extraction period, the samples are collected from the extraction vessel, filtered, and rotary-vacuumed to rid of the solvent, until the volume of the solution remains 30 % compared to the original. Next, take this solution out of the flask, remove the essence and oil from curcumin, then crystallize it with hexane. Finally, the powder sample is weighed and prepared into a 1 mg/mL solution for content analysis using the HPLC/MS method. Hence, we have samples N2, N3, N4, N5, N5-1 and N12, N13.
Characterization techniques for curcumin
The fabricated curcumin is analyzed with high performance liquid chromatography/mass spectrometry (HPLC/MS) of Agilent 1260 Series Single Quadrupole LC/MS Systems (Agilent Technologies, USA). Stock solutions of curcumin (200 μg/mL) was prepared by dissolving accurately weighed 10 mg of curcumin in methanol using 50 mL volumetric flask. This solution was further diluted with methanol to obtain standard solutions in the concentration range of 1–20 μg/mL. The separation was carried out in a Zorbax Eclipse XDB C18 column (4.6 × 150 mm, 5 µm) with a C18 guard column maintained at 24 °C. The elution was performed at flow rate of 0.5 mL/min with a gradient mobile phase from 10 to 30 % acetonitrile in water in 5 min, followed by an increase from 30 to 70 % acetonitrile in another 20 min. The injection volume was of 5 µL. The DAD acquisition wavelength for demethoxycurcumin, bisdemethoxycurcumin and curcumin detection was set at 425 nm. The mass detector used a MM-ES multimode source. OpenLAB software (Agilent Technologies) was employed to control and collect sample. The ion peaks at m/z 339.0, 309.1, and 369.1 ([M + H]+) were detected for demethoxycurcumin, bisdemethoxycurcumin and curcumin, respectively.
Moreover, the curcumin samples are analyzed by Micro Raman spectroscopy (Explore-Horiba) using 785 nm excitation line from a diode pumped, solid state laser at 100 mW to analyze the vibrational bonds and their Raman frequencies. 10× Objectives are used to focus the excitation laser light on the right spot of the investigated samples, the spot size of the laser beam is 1 μm, spectral resolution is 2 cm−1, and acquisition time is always 10 s.
X-ray powder diffraction (XRD) method (Siemens D5005) is used to identify curcumin crystalline phase in the samples. The UV–Vis spectra of curcumin in ethanol are scanned within the wavelength range of 200–600 nm using a Shimadzu (UV-1800) UV–Vis spectrophotometer. All UV–Vis measurements are performed at 25 °C and automatically corrected for the solvent medium, which is the absolute ethanol. The photoluminescence (PL) spectra of curcumin deposited on glass slit are recorded at room temperature on a Fluorolog-3 Model FL3-22 spectrofluorometer system (HORIBA Jobin–Yvon). The emission spectra are measured utilizing a 450 W xenon lamp as the excitation source.
Results and discussion
HPLC method for curcumin analysis
The peaks of demethoxycurcumin (II), bisdemethoxycurcumin (III) and curcumin (I) are spotted based on the match in the retention time Rt and mass number MS compared to the indicator substance. The mass percentages of the three compounds and content analysis result of the substances are calculated.
We have carried out content analysis of curcumin in some market products, which are named samples N6 and N7 (two samples of the same manufacturer with the same label, and both are nano curcumin according to the company’s instructions), N8 which only contains curcumin and neither demethoxycurcumin (II) nor bisdemethoxycurcumin (III), N9 and N10 which contain a certain amount of form II and form III. The sample N11 is also a commercial nano cucumin product. These data can be used as references to compare. The analysis data by HPLC/MS method shows that the curcumin samples N1 (54 %), N4 (41.66 %), N5-1 (41.43 %) and N12 (56.1 %) have superior quality, and can be compared to those being sold on the market.
Curcumin characterization by Micro-Raman and X-ray diffraction
Vibration Raman spectrum is a strong tool to define important information on molecular structures of a chemical substance, especially the chemical molecules of a natural product, since they show some vibration modes of some chemical bonds characteristic of the substance itself. On the other hand, if the substance also exists in crystalline powder form, we can also identify the crystalline phase of that substance by the X-ray diffraction (XRD) method. Therefore, we have begun to register micro-Raman spectra and XRD in order to have structural information on curcumin and the crystalline phase of fabricated powder samples under the extracting conditions of room temperature and in a microwave with different power levels and time periods.
Experimental Raman (crystalline powder) spectral data of curcumin in frequency region 1700–900 cm−1
Cur N3-1 6 months later
Mangolim et al. (2014)
Kolev et al. (2005)
Kolev et al. (2005)
ν C=O (II)
ν C=O (III)
ν C=O (I) νC=C
ν C=C (I, II) Aromatic
νC=C (II, III) Aromatic
Phenol C–O (I)
Phenol C–O (II, III)
Enol C–O (I)
Enol C–O (II, III)
Similar to observations in Fig. 1, we can see that the vibration spectra of fabricated natural product samples from N1 to N4 all possess nearly the same characteristic lines and similar to those recorded from fresh turmeric grown in Northern Vietnam. The vibration spectra of this fresh turmeric are similar to the Raman spectra of those reported before (Bich et al. 2009). The vibration spectra of sample N6 is very similar to those of the fabricated samples and have been presented in this paper. Comparing to the Raman spectrum of the sample N8, which only contains curcumin (I), we see that other than the observed vibration lines at frequencies being the same, there are two lines that are characteristic of curcumin’s vibration frequencies (I) whose peak, in samples containing curcumin (II) and (III), is shifted towards frequencies with larger values. Observed quite clearly, being characteristic of curcumin form I chemical structure, they are lines that appear at frequency 959 cm−1—characteristic of vibration ν(C=O) stretching, and at frequency 1625 cm−1—characteristic of vibration ν (C=O) stretching or C=C stretching (form I) of curcumin (Kolev et al. 2005; Sanphui et al. 2011; Krishna Mohan et al. 2012). From the detailed analysis of the positions of these two vibration lines, combined with curcumin I, II, III values defined using the HPLC/MS method and the positions of these two peaks on the Raman spectra, it can be concluded—in a quantitative way right after recording the Raman spectrum of a sample—that the sample contains more or less curcumin form I. From comparing positions of frequencies at 959 and 1625 cm−1, it clarifies that in the series of samples for production research, sample N1 contains the most curcumin with higher quality compared to the rest. However, sample N6 may contain less curcumin I than samples N8 and N1, despite having the exact same origin as samples fabricated from turmeric in this research (Fig. 2).
We have also carried out experiments to clean, crystallize and re-crystallize curcumin multiple times with fabricated samples. The Raman measurement results show that the Raman spectra do not change after crystallizations. With the same sample N1 kept for 6 months, we dissolved, re-crystallized and recorded its Raman spectra to find that the Raman spectra of these samples remain completely unchanged, and still the same as those of the original sample N1 and fresh turmeric.
Absorption and PL spectra of curcumin
The more diluted the curcumin solution is, the more the UV–Vis absorption intensity decreases. The main reason the absorbance decreases in aqueous solution when concentration decreases is the decrease in the number of absorption centers, while on the other hand it is also the degradation of curcumin in water medium by a reaction at the keto-enol group (Singh et al. 2014).
We have succeeded in fabricating a large amount of curcumin from turmeric of northern Vietnam. The extraction method, with the help of microwave technology, saved much extraction time and produced high-quality curcumin which can be compared with the nano curcumin available on the Vietnamese market. Furthermore, the study also allows evaluation of the curcumin sample fabricated by extracting with ethanol at room temperature. This research on Raman and PL spectra allows the curcumin quality evaluation as well as comparison and identification of the fabricated and commercial products on the Vietnamese market. The study of Raman spectra shows that it is possible to use the two vibration lines at frequencies ~959 and ~1625 cm−1 to evaluate the quality of the curcumin sample as well as identifying curcumin of natural or synthesis origins.
The research has pointed out that our fabricated curcumin has achieved the first step, which is being made from Vietnamese yellow turmeric (Curcuma Longa Linn), has quality that can be compared well to those being sold on the Vietnamese market, and stable after being maintained for 6 months at room temperature. Our fabricated curcumin product serves well for being a natural initial material in nanotechnology to fabricate nanocurcumin used in the pharmaceutical industry, healing burns, cosmetics, functional food and foods.
TVT and HVN contributed in buying the input turmeric supply for the curcumin production process as well as taking responsibility in washing and cleaning turmeric roots, peeling and cutting them into turmeric threads, then processing the convention extraction and microwave. HVN, VDC take responsibility in the rotary evaporation process and cleansing the products after rotary evaporation. PNT contributed in analyzing the products by the HPLC and absorption methods. LVV carried out X-Ray Diffraction (XRD) measurements on curcumin powder samples. LXH and PTD recorded the fluorescent and Raman spectra of all fabricated curcumin samples. PTN is in charge of designing the whole experiment, processing the obtained data and discuss the results with the whole team as well as writing the manuscript. All authors read and approved the final manuscript.
The authors thank the National Key Laboratory for Electronic Materials and Devices of Institute of Materials Science (IMS), DAST Company and Duy Tan University for the use of facilities. We sincerely thank Profs. Nguyen Quang Liem, Luu Minh Dai, Vũ Xuan Quang, Nguyen Thi Thuc Hien and Dr. Dao Ngoc Nhiem (IMS) for the help and precious discussion.
The authors declare that they have no competing interests
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