Altered expression of the mismatch repair genes in DF-1 cells infected with the avian leukosis virus subgroup A
© The Author(s) 2016
Received: 12 May 2016
Accepted: 29 September 2016
Published: 7 October 2016
The absence or deficiency of DNA mismatch repair (MMR) activity results in microsatellite instability (MSI) in cancer. The avian leukosis virus (ALV) causes neoplastic disease in chickens. In this study, the status of MMR, MSI, the cell cycle and apoptosis were detected in DF-1 cells after avian leukosis virus subgroup A infection. Flow cytometry analysis results indicated that there was no significant difference in cell apoptosis between the control and infected groups. The percentage of cells in S and G2 phases were increased in the infected group. MSI and mutation of MSH2 and MLH1 gene exons were absent in DF-1 cells after infection. Levels of MSH2 and MLH1 mRNA were dramatically increased in DF-1 cells after infection. These results demonstrated that ALV RAV-1 infection may promote the expression of MSH2 and MLH1 genes rather than resulting in gene mutations. Mismatch repair functions were normal and may be have relationships with the arrest of S phase and G2 phase.
KeywordsALV DF-1 cells Microsatellite instability Mismatch repair
DNA mismatch repair (MMR) is a highly conserved pathway from yeast to humans (Klucking et al. 2005). It contributes to DNA replication fidelity by recognizing mis incorporation errors and facilitating their excision. Several MMR proteins have been identified in eukaryotic cells include the MutS homologs (MutSα and MutSβ) and the MutL homologs (MutLα and MutLβ) (Hsieh and Yamane 2008). MutSα, MSH2–MSH6, recognizes single base mismatches and small loops in the DNA. MutSβ, MSH2-MSH3, recognizes lager loops. MLH1 and PMS2 bind to form MutL protein, known as MutLα, in mammalian cells. The formation of a ternary complex consisting of MutSα, MutLα and a mismatched DNA signals the start of the excision step of MMR. The following steps include strand discrimination, excision, resynthesis, and ligation by exonuclease I (EXO1), proliferating cell nuclear antigen (PCNA), replication protein A (RPA), single strand DNA-binding protein (SSB), high mobility group protein B1(HMGB1), DNA pol δ, DNA ligase I and so on.
The absence or deficiency of DNA MMR activity can developed instability in simple sequence repeats, that known as microsatellite instability (MSI). MSI can be detected by isolation of the DNA from a tumor specimen that has defective DNA MMR activity. The microsatellite sequence was amplified by polymerase chain reaction (PCR), and compared with normal DNA of that individual. The DNA of the cancerous cell contains deletions or addition in the microsatellite sequence, which can serve as a surrogate for the absence of DNA MMR activity. Some microsatellite sequences occur in coding regions and mutations in target genes actually mediate neoplastic behavior, This phenotype was first found in Lynch syndrome tumors (Boland et al. 1998). Germline mutations in one of the four major HNPCC-associated MMR genes; i.e., MLH1, MSH2, MSH6, and PMS2, are detected in up to 70–80 % of such families (Peltomaki 2003). MMR deficiency is a common feature of many types of tumors i.e.. Loss of expression from one of the MMR proteins (MSH2, MSH6, MSH3, MLH1, and PMS2) which is associated with an increased predisposition to colorectal, gastric, endometrial, ovarian, and other cancers (Guillotin and Martin 2014; McConechy et al. 2015; Shilpa et al. 2014; van Grieken et al. 2013). MSI is also observed in a subset of tumors induced with viral infections. EBV DNase caused chromosomal aberrations and increased the MSI and frequency of genetic mutations in human epithelial cells (Wu et al. 2010). The hepatitis C virus core protein promoted microsatellite instability in PH5CH8 cells. MSI was a real event occurring in primary chicken embryo fibroblasts and in chickens with lymphoma infected with MDV (Yao et al. 2013; Zhou et al. 2012).
The avian leukosis virus (ALVs), which belong to the genus alpha retrovirus of the Retroviridae family, cause neoplastic diseases and other reproduction problems in the poultry industry worldwide (Gao et al. 2012). According to the host range, the antigen structure of glycoproteins on the virus envelope and the interference between different strains in cell culture, avian leukosis viruses are divided into 10 subgroups (A-J), with six subgroups (A, B, C, D, E and J) existing in chickens (Dai et al. 2015; Reinisova et al. 2008; Yang et al. 2011). The subgroups A, B and J are mainly exogenous avian leukosis viruses that cause avian tumors, while subgroup E is a non-pathogenic or low pathogenic endogenous avian leukosis virus (Dai et al. 2015).
In this paper, we address the question of whether MMR deficiency and MSI are present in avian tumors induced with avian leukosis virus subgroup A. We describe the status of MSI, the MMR system (MSH2 and MLH1), the cell cycle and apoptosis in DF-1 cells infected with avian leukosis virus subgroup A. Our data revealed that infection with ALV RAV-1 could cause confusion in the cell cycle and increase the expression of MSH2 and MLH1 mRNA. There was no occurrence of MSI on the selected 16 microsatellite sites and no mutations of exons in the MSH2 and MLH1 genes.
DF-1 cells were grown in 25 cm2 flasks with Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific Inc.) supplemented with 10 % fetal bovine serum (FBS) and 1 % antibiotics (penicillin, streptomycin) and were maintained at 37 °C in an incubator under an atmosphere of 5 % CO2.
Avian leukosis virus subgroup A, ALV RAV-1(CAVCC AV228), was purchased from the China Veterinary Culture Collection Center. After expanding the culture of the DF-1 cell line in our laboratory, each 0.1 mL cell supernatant contained 104.75 TCID50.
Infection of DF-1 with ALV RAV-1
The DF-1 cells were seeded (approximately 1 × 105 cells/well) in 6-well culture plates. The cells of the infected group were infected with 100 μL ALV RAV-1 (n = 3). After 2 h incubation, the cells were washed and maintained in media with 1 % FBS for 7 days. The cells of the control group were supplemented with 100 μL DMEM at the same time (n = 3).
Specific p27 antigen test
Samples of 150 µL of cell supernatants were collected at 7 days post infection (d.p.i.), and ALV-p27 antigen was detected with an Avian Leukosis Virus Antigen Test Kit (IDEXX USA Inc.) according to the manufacturer’s instructions.
Cell cycle and apoptosis analysis by flow cytometry (FCM)
For cell cycle analysis, cells at 7 days post infection were fixed with ice cold 75 % ethanol and were treated with RNase A (50 μg/mL) and 0.05 % Triton X for 20 min at room temperature. Subsequently, PI (50 μg/mL) was added to the RNase treated cell suspension and incubated for 15 min in the dark before analysis with FCM (BD FACS Calibur, BD, USA). Data were analyzed using Cell Quest Pro Software. Cells were washed twice with ice-cold PBS. The levels of apoptosis were determined by flow cytometric method after Annexin V-FITC/propidium iodide (PI) double staining using an Annexin V-FITC Apoptosis Detection Kit (KeyGEN BioTECH, China) according to the manufacturer’s instructions.
Detection of microsatellite instability
Cells were harvested at 7 days post infection (d.p.i.). DNA extraction and PCR amplification were performed according to previous reports (Zhou et al. 2012). The 16 microsatellite markers used in this research were ABR0008, ABR0547, ABR0161, ABR0587, ABR0520, ABR0197, ABR0236, ABR0563, ABR0189, ABR0579, MCW0311, MCW0027, MCW0220, ADL0217, LEI0223, and LEI0096. The primers information were reported previously (Yao et al. 2013).
Detection of exon mutations of the mismatch repair gene
Primers used to amplify exon of MSH2 gene
Size of fragments amplified (bp)
MSH2 exon1 F
MSH2 exon1 R
MSH2 exon2 F
MSH2 exon2 R
MSH2 exon3 F
MSH2 exon3 R
MSH2 exon4 F
MSH2 exon4 R
MSH2 exon5 F
MSH2 exon5 R
MSH2 exon6 F
MSH2 exon6 R
MSH2 exon7 F
MSH2 exon7 R
MSH2 exon8 F
MSH2 exon8 R
MSH2 exon9 F
MSH2 exon9 R
MSH2 exon10 F
MSH2 exon10 R
MSH2 exon11 F
MSH2 exon11 R
MSH2 exon12 F
MSH2 exon12 R
MSH2 exon13 F
MSH2 exon13 R
MSH2 exon14 F
MSH2 exon14 R
MSH2 exon15 F
MSH2 exon15 R
MSH2 exon16 F
MSH2 exon16 R
Primers used to amplify exon of MLH1 gene
Size of fragments amplified (bp)
MLH1 exon1 F
MLH1 exon1 R
MLH1 exon2 F
MLH1 exon2 R
MLH1 exon3 F
MLH1 exon3 R
MLH1 exon4 F
MLH1 exon4 R
MLH1 exon5 F
MLH1 exon5 R
MLH1 exon6 F
MLH1 exon6 R
MLH1 exon7, 8 F
MLH1 exon7, 8 R
MLH1 exon9 F
MLH1 exon9 R
MLH1 exon10 F
MLH1 exon10 R
MLH1 exon11 F
MLH1 exon11 R
MLH1 exon12 F
MLH1 exon12 R
MLH1 exon13 F
MLH1 exon13 R
MLH1 exon14 F
MLH1 exon14 R
MLH1 exon15 F
MLH1 exon15 R
MLH1 exon16 F
MLH1 exon16 R
MLH1 exon17 F
MLH1 exon17 R
MLH1 exon18 F
MLH1 exon18 R
MLH1 exon19 F
MLH1 exon19 R
Mismatch repair-related gene expression
The primers used for real-time PCR
Size of fragments amplified (bp)
β-acting mRNA F
β-acting mRNA R
MSH2 mRNA F
MSH2 mRNA R
MLH1 mRNA F
MLH1 mRNA R
Cell supernatant p27 antigen S/P value
Viral infection was confirmed by measuring the ALV-p27 antigen levels with an ELISA. DF-1 cells were infected with ALV RAV-1 and maintained in culture for 7 days. The cell supernatant was collected at 7 d.p.i. The S/P values of the infected group were between 0.551 and 0.920 (positive critical value was 0.2), and the S/P values of the control group were between 0.065 and 0.153. If the S/P value was more than the critical value 0.2, the p27 antigen was positive.
Cell cycle and apoptosis results
The results of cell cycle
Control (n = 3)
82.17 ± 1.37a
4.06 ± 0.46b
13.77 ± 0.93b
Infected (n = 3)
78.48 ± 1.49b
5.03 ± 0.43a
16.48 ± 1.36a
The results of the apoptosis rate
Control (n = 3)
10.00 ± 0.51a
Infected (n = 3)
10.62 ± 0.93a
Microsatellite instability test results
Detection of mutation of MSH2 and MLH1 gene exons
Mismatch repair-related gene expression
DF-1 cells are a chicken fibroblast cell line. DF-1 cells were derived from ELL chicken embryos and appeared to have a fibrous form, containing no avian leukosis virus endogenous genes (Himly et al. 1998). Endogenous ALVs could not proliferate in DF-1 cells. Therefore, we selected this cell line to reduce the effects of endogenous factors and further decrease the false positive rate in this test.
ALV can replicate and proliferate in DF-1 cells without cytopathic effects. P27 antigen is a specific antigen of the avian leukosis virus group (Yun et al. 2013). It can be produced by both exogenous viruses and endogenous viruses in infected cells, and there are no differences in the p27 antigens of different subgroups, except for the expression levels. A commercialized p27 antigen ELISA kit was used in this study to confirm infection with ALV RAV-1. ALV RAV-1 was used to infect DF-1 cells and the culture was maintained for 7 days. The S/P value of the infected group was higher than the critical value 0.2, which suggests that ALV RAV-1 successfully infected the DF-1 cells.
Most previous studies reported that there were no obvious cytopathic effects in CEFs or DF-1 cells after ALV-A infection. DF-1 cells overexpressed TVA, a low-density lipoprotein receptor-related protein, become susceptible to ALV-A induced CPE. ALV-A cytopathic effects is associated with higher levels of viral superinfection and DNA accumulation(Kolodner and Marsischky 1999). In this study, flow cytometry was utilized to evaluate DF-1 cells apoptosis. The results revealed that ALV RAV-1 did not induce cell apoptosis. Gai et al. reported ALV-A and Radish anthocyanin had a significant impact on promoting the biological activity of DF-1 cells using an MTT assay (Lili 2012). These results were contrary to previous reports. Wang et al. reported that ALV-A induced cell apoptosis in vitro using an MTT assay. The death of cells induced by ALV-A can be inhibited by adding anthocyanins from purple corn (Wang et al. 2014). In addition, it has also been reported that different results were observed in immune cells. It is likely that ALV-J infection induced apoptosis in dendritic cells (Liu et al. 2016).
Although ALV RAV-1 infection could not induce apoptosis in DF-1 cells, the flow cytometry cell cycle analysis indicated that ALV RAV-1 infection can inhibit cell cycle progression. The percentages of cells in S phase and G2 phase were increased after ALV RAV-1 infection. This suggests that cell cycle arrest occurred at S phase and G2 phase. In a previous study, a time- and dose-dependent G2/M arrest after Na2SeO3 and MSeA exposure was reported in HCT116 + hMLH1 cells, but not in HCT116 cells (Qi et al. 2010). This suggested that G2 arrest was dependent on hMLH1 and was regulated by ATM and ROS. In this study, the real-time PCR results indicate that expression of the DNA mismatch repair genes mRNA (MSH2 and MLH1) were increased after ALV RAV-1 infection. Up-regulation of MSH2 and MLH1 mRNA may have certain relationships with cycle of arrest S phase and G2 phase, and with the DNA repair of damage in DF-1 cells after ALV RAV-1 infection.
Defective MMR results in abnormalities in the processes of DNA repair. The differential instability at microsatellites emphasizes the heterogeneity of phenotypes associated with MMR. Exon mutations of MSH2 and MLH1 genes were not detected by non-denaturing polyacrylamide gel electrophoresis. The results indicate no mutation was found in MSH2 and MLH1 gene exons in DF-1 cells infected with ALV RAV-1. Meanwhile, there was no MSI of the 16 microsatellite loci in the genomic DNA of DF-1 cells infected with ALV RAV-1. These results suggest that the function of MMR in DF-1 cells infected with ALV RAV-1 was normal.
In conclusion, our results indicate that ALV RAV-1 infection may promote the expression of MSH2 and MLH1 mRNA rather than causing gene mutations. The DF-1 cells were arrested in S phase and G2 phase after the ALV RAV-1 infection.
Conceived and designed the experiments: DJY DWY. Performed the experiments: LZ YH JL. Analyzed the data: DWY JX. Contributed reagents/materials/analysis tools: LZ YH JL. Wrote the paper: LZ JX DWY. All authors read and approved the final manuscript.
This study was supported from the Natural Science Foundation of Jiangsu Province (BK20130686), National Natural Science Foundation of China (30871847), Ministry of Agriculture 948 Project (2011-z20) and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
The authors declare that they have no competing interests.
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- Boland CR, Thibodeau SN, Hamilton SR, Sidransky D, Eshleman JR, Burt RW, Meltzer SJ, Rodriguez-Bigas MA, Fodde R, Ranzani GN, Srivastava S (1998) A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res 58:5248–5257PubMedGoogle Scholar
- Dai M, Feng M, Liu D, Cao W, Liao M (2015) Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B. Virol J 12:52View ArticlePubMedPubMed CentralGoogle Scholar
- Gao Y, Yun B, Qin L, Pan W, Qu Y, Liu Z, Wang Y, Qi X, Gao H, Wang X (2012) Molecular epidemiology of avian leukosis virus subgroup J in layer flocks in China. J Clin Microbiol 50:953–960View ArticlePubMedPubMed CentralGoogle Scholar
- Guillotin D, Martin SA (2014) Exploiting DNA mismatch repair deficiency as a therapeutic strategy. Exp Cell Res 329:110–115View ArticlePubMedGoogle Scholar
- Himly M, Foster DN, Bottoli I, Iacovoni JS, Vogt PK (1998) The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses. Virology 248:295–304View ArticlePubMedGoogle Scholar
- Hsieh P, Yamane K (2008) DNA mismatch repair: molecular mechanism, cancer, and ageing. Mech Ageing Dev 129:391–407View ArticlePubMedPubMed CentralGoogle Scholar
- Klucking S, Collins AS, Young JAT (2005) Avian sarcoma and leukosis virus cytopathic effect in the absence of TVB death domain signaling. J Virol 79:8243–8248View ArticlePubMedPubMed CentralGoogle Scholar
- Kolodner RD, Marsischky GT (1999) Eukaryotic DNA mismatch repair. Curr Opin Genet Dev 9:89–96View ArticlePubMedGoogle Scholar
- Lili G (2012) The impact of different species sources of anthocyanins on the biological activity of DF-1 cell line which infected by avian leukemia virus subgroup A. College of Animal Science and Veterinary Medicine. Shandong Agricultural University, Shandong, pp 59–62Google Scholar
- Liu D, Dai M, Zhang X, Cao W, Liao M (2016) Subgroup J avian leukosis virus infection of chicken dendritic cells induces apoptosis via the aberrant expression of microRNAs. Sci Rep 6:20188ADSView ArticlePubMedPubMed CentralGoogle Scholar
- McConechy MK, Talhouk A, Li-Chang HH, Leung S, Huntsman DG, Gilks CB, McAlpine JN (2015) Detection of DNA mismatch repair (MMR) deficiencies by immunohistochemistry can effectively diagnose the microsatellite instability (MSI) phenotype in endometrial carcinomas. Gynecol Oncol 137:306–310View ArticlePubMedGoogle Scholar
- Peltomaki P (2003) Role of DNA mismatch repair defects in the pathogenesis of human cancer. J Clin Oncol 21:1174–1179View ArticlePubMedGoogle Scholar
- Qi Y, Schoene NW, Lartey FM, Cheng W-H (2010) Selenium compounds activate ATM-dependent DNA damage response via the mismatch repair protein hMLH1 in colorectal cancer cells. J Biol Chem 285:33010–33017View ArticlePubMedPubMed CentralGoogle Scholar
- Reinisova M, Senigl F, Yin X, Plachy J, Geryk J, Elleder D, Svoboda J, Federspiel MJ, Hejnar J (2008) A single-amino-acid substitution in the Tvb(S1) receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo. J Virol 82:2097–2105View ArticlePubMedGoogle Scholar
- Shilpa V, Bhagat R, Premalata CS, Pallavi VR, Krishnamoorthy L (2014) Microsatellite instability, promoter methylation and protein expression of the DNA mismatch repair genes in epithelial ovarian cancer. Genomics 104:257–263View ArticleGoogle Scholar
- van Grieken NCT, Aoyma T, Chambers PA, Bottomley D, Ward LC, Inam I, Buffart TE, Das K, Lim T, Pang B, Zhang SL, Tan IB, Carvalho B, Heideman DAM, Miyagi Y, Kameda Y, Arai T, Meijer GA, Tsuburaya A, Tan P, Yoshikawa T, Grabsch HI (2013) KRAS and BRAF mutations are rare and related to DNA mismatch repair deficiency in gastric cancer from the East and the West: results from a large international multicentre study. Br J Cancer 108:1495–1501View ArticlePubMedPubMed CentralGoogle Scholar
- Wang D, Lei Y, Ma Y, Zhang L, Zhao X (2014) Inhibition of ALV-A-induced apoptosis in DF-1 cells via inactivation of nuclear transcription factor kappa S by anthocyanins from purple corn (Zea mays L.). J Funct Foods 10:274–282View ArticleGoogle Scholar
- Wu C-C, Liu M-T, Chang Y-T, Fang C-Y, Chou S-P, Liao H-W, Kuo K-L, Hsu S-L, Chen Y-R, Wang P-W, Chen Y-L, Chuang H-Y, Lee C-H, Chen M, Chang W-SW, Chen J-Y (2010) Epstein-Barr Virus DNase (BGLF5) induces genomic instability in human epithelial cells. Nucleic Acids Res 38:1932–1949View ArticlePubMedGoogle Scholar
- Yang J, Yu Y, Yao J, Chen Y, Xu G, Yang N, Sun D, Zhang Y (2011) Molecular identification of avian leukosis virus subgroup E loci and tumor virus B locus in Chinese indigenous chickens. Poult Sci 90:759–765View ArticlePubMedGoogle Scholar
- Yao D-W, Xu J-R, Zhou Z-L, Li S-T, Yang D-J (2013) Microsatellite instability in chicken lymphoma induced by gallid herpesvirus 2. Plos One 8:e68058ADSView ArticlePubMedPubMed CentralGoogle Scholar
- Yun B, Li D, Zhu H, Liu W, Qin L, Liu Z, Wu G, Wang Y, Qi X, Gao H, Wang X, Gao Y (2013) Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. J Virol Methods 187:278–283View ArticlePubMedGoogle Scholar
- Zhou Z, Yao D, Qiu Y, Yang D (2012) Microsatellite instability in Marek’s Disease Virus infected primary chicken embryo fibroblasts. Virol J 9:193View ArticlePubMedPubMed CentralGoogle Scholar