- Open Access
BCL-2 inhibition impairs mitochondrial function and targets oral tongue squamous cell carcinoma
© The Author(s) 2016
- Received: 26 April 2016
- Accepted: 12 September 2016
- Published: 21 September 2016
To understand the role of Bcl-2 overexpression in oral tongue squamous cell carcinoma (OTSCC) patients and investigate the efficacy of targeting Bcl-2 in OTSCC.
The expression level of Bcl-2 on normal tongue cells and OTSCC cells were measured by real-time PCR and western blotting. The functional roles of Bcl-2 were examined by MTS, flow cytometry and xenograft cancer mouse model. Mechanism studies were performed by analyzing mitochondrial functions in a panel of OTSCC cell lines.
Bcl-2 is up-regulated at mRNA and protein levels in a panel of OTSCC cell lines compared to normal tongue epithelial cells (NTEC). Importantly, overexpression of Bcl-2 confers resistance of OTSCC cells to chemotherapeutic drug cisplatin treatment. Overexpression of Bcl-2 in NTEC significantly increased cell growth. In contrast, inhibition of Bcl-2 by genetic and pharmacological approaches inhibits proliferation and induces apoptosis in OTSCC cells. Mechanistically, Bcl-2 inhibitor ABT-199 impairs mitochondrial functions as shown by the decreased levels of mitochondrial membrane potential, mitochondrial respiration and ATP, and the increased levels of ROS in OTSCC cells. In addition, ABT-199 inhibits proliferation and induces apoptosis and mitochondrial dysfunctions in NTEC cells, but to a less extent than in OTSCC cells. We further show that ABT-199 augments the effects of cisplatin in eliminating OTSCC cells in in vitro tongue cancer cellular system and in vivo tongue cancer xenograft mouse model.
Inhibition of Bcl-2 effectively targets OTSCC cells through inhibiting proliferation and inducing apoptosis. Inhibition of Bcl-2 also augments the inhibitory effects of cisplatin in vitro and in vivo.
- Tongue squamous carcinoma
The clinical management of the majority of the oral cancer patients are still challenge with the 5-year overall survival and disease-free survival remaining ~55 and ~60 % (Goldstein et al. 2013). Oral tongue squamous cell carcinoma (OTSCC) is a subtype of oral cancer, which is more clinically aggressive with rapid local invasion and a high recurrence rate (Tan et al. 2012). OTSCC has increased incidence over the last several years and poor prognosis (Garnaes et al. 2015). Current treatment for OTSCC include surgery (using microvascular reconstructive techniques), radiotherapy (e.g. external beam radiotherapy and brachytherapy), chemotherapy (e.g. cisplatin) and various combinations of these modalities depending on the disease stages and presentations (Ferlay et al. 2015; Shiboski et al. 2005; Huang and O’Sullivan 2013; Andreadis et al. 2003). The molecular pathogenesis of OTSCC and its underlying mechanisms to chemotherapy resistance are not well understood. Research have found that epigenetic and genetic factors, such as oncogenes (e.g. Ras) and tumor suppressor genes (e.g. p53), can significantly influence the development of OTSCC (Khan and Bisen 2013; Murugan et al. 2012). It is therefore important to elucidate the mechanisms involved in the resistance and identify effective targets for patients with OTSCC.
Activation of pro-survival B cell lymphoma 2 (BCL2) family genes (e.g. MCL1, BCL2 and BCLX) is common hallmark of cancer and contributes to tumorigenesis via BCL2-mediated apoptosis (Adams and Cory 2007). BCL-2 is transcriptionally up-regulated in response to cytokines or pathways involved in proliferation, such as PI3K/AKT and Ras (Kinoshita et al. 1995; Franke et al. 2003). Downregulation of Bcl-2 by using ABT-199, a potent and selective inhibitor of Bcl-2 (Souers et al. 2013), has been shown to inhibit growth of a panel of cancers (Ko et al. 2014; Goff et al. 2013). Several studies have been shown that the expression of Bcl-2 family proteins are associated with clinical stage, histologic grade and poor prognosis in OTSCC patients (Camisasca et al. 2009; de Vicente et al. 2006; Zhang et al. 2012). However, little is known about the functional roles of Bcl-2 in OTSCC.
In this study, we investigated the expression and roles of Bcl-2 in normal tongue cells and multiple OTSCC cell lines. Our results show that Bcl-2 is up-regulated in OTSCC compared to normal tongue cells. The up-regulation of Bcl-2 contributes to the resistance of OTSCC to chemotherapeutic drug treatment. We further show the essential roles of Bcl-2 in growth and survival of OTSCC cells. In addition, Bcl-2 inhibition effectively inhibits proliferation and induces apoptosis of OTSCC via impairing mitochondrial functions. Finally, we demonstrate that the combination of BCL-2 inhibitor ABT-199 and chemotherapeutic drug cisplatin are more effective than single drug alone in targeting OTSCC both in vitro and in vivo.
Cell culture and drugs
Human primary cultured normal tongue epithelial cells (NTEC, a kind gift from Dr. Sun’s laboratory and Dr. Zeng’s laboratory) (Wen et al. 2014; Song et al. 2006) were maintained in keratinocyte/serum-free medium (Invitrogen Life Technologies, US). Oral tongue squamous cell carcinoma (OTSCC) cell lines SCC-9 and SCC-25 were purchased from the American Type Culture Collection. Tca8113 and CAL27 were purchased from the Committee of the Type Culture Collection of the Chinese Academy of Sciences. Cells were cultured in RPMI 1640 medium supplemented with 10 % fetal bovine serum (HyClone, UK). ABT-199 (Catalogue No. CT-A199) and cisplatin (Catalogue No. 479306) were purchased from ChemieTek and Sigma, respectively.
Plasmid and siRNA transfection
For Bcl-2 overexpression, OTSCC cell lines were transfected with 1.5 µg pEMD or pEMD-Bcl2 plasmids (a kind gift from Clark Distelhorst) as previously described (Wang et al. 2001). BCL-2 knockdown were carried out in TSCC lines by transfecting with 100 nM scramble siRNA or human BCL-2-specific siRNAs using Dharmafect Transfection Reagent (Dharmacon RNAi Technologies) according to manufacture’s instructions. The target sequences of human BCL-2-specific are siRNA BCL-2: AAC ATC GCC CTG TGG ATG ACT.
Cellular protein were extracted by RIPA lysis buffer (50 mM Tris HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Nonidet P-40, 1 mM Na3VO4, 1 mM NaF, and 1× protease inhibitor, Life Technologies Inc, US). Total protein content was measured using the bicinchoninic acid protein assay kit (Thermo Scientific, US). Equal amount of proteins were resolved using denaturing sodium dodecyl sulphate–polyacrylamide gel electrophoresis and analysed by western blot using antibodies against BCL-2 and β-catenin (Cell Signalling Technologies, US).
Real-time PCR analysis
The total RNA of NTEC, SCC-25, SCC-9, CAL27 and Tca8113 cells were isolated by using TRIzol Reagent (Life technologies, CA, US). The cDNA was amplified using a SsoFast EvaGreen Supermix kit (Bio-rad. CA) and real time PCR analysis was then performed using CFX96 RT PCR system (Bio-rad, CA). The primers are for human BCL-2 (5′-CTG CAC CTG ACG CCC TTC ACC-3′ and 5′-CAC ATG ACC CCA CCG AAC TCA AAG A-3′) and β-actin (5′-AAG GAT TCC TAT GTG GGC GAC G-3′ and 5′-GCC TGG ATA GCA ACG TAC ATG G-3′).
Measurement of proliferation and apoptosis
Cells were treated with ABT-199, cisplatin or combination for 3 days. Proliferation activity was measured by the CellTiter 96R AQueous One Solution Cell Proliferation assay kit (Promega, US). To monitor cell density, 1 × 105 cells were seeded on Day 1. The cell density were counted daily for 4 days under microscope. We determined the cell density by using Trypan Blue staining and then hemocytometer. Briefly, we take the average cell count from each of the sets of 16 corner squares and multiply by 10,000; then multiply by 2 to correct for the 1:2 dilution from the Trypan Blue addition.
Apoptotic cells were stained with Annexin V-FITC (Beckman Coulter, France) and analysed on a Beckman Coulter. The percentage of Annexin V-positive cells was determined by using CXP software.
Determination of mitochondrial membrane potential, oxygen consumption rate (OCR), reactive oxygen species (ROS) and cellular ATP level
Cells were treated with ABT-199 for 24 h. For mitochondrial membrane potential measurement, the cells were incubated with 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl benzimidazolylcarbocyanine iodide (JC-1, Invitrogen) for 30 min prior to PBS washing. Labelled cells were resuspended in 500 µL PBS and flow cytometry was conducted on a Beckman Coulter FC500. The mitochondrial membrane potential level was analysed using FlowJo version 7.7.1 (TreeStar, Ashland, OR). OCR was measured using a Seahorse XF24 extracellular flux analyser (Seahorse Bioscience, US) as per manufacturer’s instructions (Varum et al. 2011). Cells in 24-well-plate were equilibrated to the un-buffered medium in a CO2-free incubator and then transferred to the Seahorse XF24 extracellular flux analyser for OCR measurement. To measure ROS levels, cells were stained with redox-sensitive probe Carboxy-H2DCFDA in PBS buffer. Labelled cells were re-suspended in PI buffer and the level of Carboxy-H2DCFDA was measured using Beckman Coulter FC500. ATP levels were measured by CellTiter-Glo Luminescent Cell Viability Assay (Promega, WI, US) according to the manufacturer’s instructions.
Tongue cancer xenograft in SCID mouse
SCID mice were purchased from Animal Resources Centre Australia. All of the animal experiments were approved by the Institutional Animal Care and Use Committee of Yangtze University. The SCID mice were inoculated with 1 million SCC-9 cells subcutaneously in the flank. The inoculation volume (0.2 ml) comprised a 50:50 mixture of cells in growth media and Matrigel (BD Biosciences). Tumour length and width were measured every 3 days and tumor volume was estimated by applying the following equation: volume = length × width2/2. When tumors reached approximately 200 mm3, the mice were treated with vehicle control, oral cisplatin at 20 mg/kg, oral ABT-199 20 mg/kg or combination of both for 30 days. Tumor frozen section slides were fixed with 4 % paraformaldehyde (Sigma, US). The nuclei and cytoplasm were counterstained with hematoxylin and eosin (H&E). The tumor sections were observed under a confocal microscopy (Zeiss LSM 510, Germany).
All data are expressed as mean ± SD. A non-parametric Student’s t test was performed using the two-tailed distribution. A p value <0.05 was considered statistically significant.
BCL-2 is activated and confers resistance to chemotherapeutic drug treatment in tongue squamous carcinoma cells
BCL-2 inhibition by genetic and pharmacological approaches inhibits proliferation and induces apoptosis of OTSCC cells
BCL-2 inhibition impairs mitochondrial functions in OTSCCs
Bcl-2 inhibitor significantly enhances the inhibitory effects of chemotherapeutic drug cisplatin in vitro and in vivo
OTSCC is an aggressive subtype of oral cancer and is resistant to current chemotherapy. Although several clinical studies have shown that Bcl-2 expression is associated with neck lymph node metastasis and poor prognosis in oral cancer (Camisasca et al. 2009; Mallick et al. 2009; Popovic et al. 2007), the functional roles of Bcl-2 in OTSCC and whether it is involved in the resistance of OTSCC to chemotherapy are not well understood. In this work, we are the first to demonstrate that Bcl-2 is overexpressed and plays essential roles in the growth and survival of OTSCC cells. Importantly, up-regulation of Bcl-2 confers resistance of OTSCC cells to chemotherapeutic drug treatment. In addition, Bcl-2 inhibition effectively targets OTSCC cells via impairing mitochondrial functions and augments cisplatin’s effects in eliminating OTSCC cells.
Our data that the BCL-2 mRNA and protein levels are significantly increased in multiple OTSCC cell lines compared to normal tongue epithelial cells (Fig. 1a, b) are consistent with the previous study that Bcl-2 is overexpressed in primary OTSCC tissues (Popovic et al. 2007). We further extend the previous studies by demonstrating that Bcl-2 up-regulation contributes resistance of OTSCC cells to cisplatin treatment (Fig. 1e, f) and combination of BCL-2 selective inhibitor ABT-199 and cisplatin is more effective in eliminating OTSCC cells (Fig. 6). The combination of ABT-199 and cisplatin is superior to single drug alone and completely arrests OTSCC in vivo tumor growth (Fig. 7). Taken together, our study clearly demonstrates the therapeutic value of targeting BCL-2 in overcoming chemotherapy resistance in OTSCC. Compared to other BCL-2 family inhibitors (e.g. navitoclax), ABT-199 has shown efficacy with less antiplatelet activity and a more favorable therapeutic index in patients with refractory chronic myeloid leukemia (Souers et al. 2013). In lines with the earlier publications, our work suggests that ABT-199 is a useful addition to the treatment armamentarium for OTSCC.
We further demonstrate that Bcl-2 overexpression in normal tongue epithelial cells promotes the growth (Fig. 1c, d). In contrast, knockdown BCL-2 by using siRNA or inhibition of its activity by using ABT-199 inhibits proliferation and induces apoptosis of OTSCC cells (Fig. 2). Activation of pro-survival BCL-2 has been shown to play positive roles in survival in various tumors (Adams and Cory 2007; Domen et al. 1998; Jiang and Milner 2003). In agreement with this notion, we are the first to show the protective and supportive roles of Bcl-2 on growth and survival in OTSCC.
A consequence of Bcl-2 inhibition is the mitochondrial dysfunction as demonstrated by the decreased mitochondrial membrane potential, decreased mitochondrial respiration and decreased ATP levels, and the increased ROS levels in OTSCC cells treated with selective Bcl-2 inhibitor ABT-199 (Fig. 4). This finding supports the previous study that BCL-2 inhibition targets oxidative phosphorylation in leukemia stem cells (Lagadinou et al. 2013). Besides mitochondrial respiration inhibition, our study extends the previous work by demonstrating that inhibition of Bcl-2 decreases mitochondrial membrane potential, inhibits ATP production and induces ROS generation. Targeting mitochondrial metabolism has emerged as an attractive therapeutic strategy in cancer due to their unique dependence on proper mitochondrial functions (Vander Heiden 2011; Jaras and Ebert 2011; Mayevsky 2009; Hagland et al. 2007). Our work suggests that targeting mitochondria may be an alternative therapeutic strategy in OTSCC.
In conclusion, our work on the identification of essential roles of BCL-2 in OTSCC provides better understanding of the molecular mechanisms underlying resistance of OTSCC cells to chemotherapy. Our work also suggests a rationale for targeting BCL-2 and mitochondria in OTSCC treatment.
LX and XZW designed the experiments, interpreted the data and wrote the manuscript. LX, YT, ZYL and JD performed the experiments. All authors read and approved the final manuscript.
We thank Dr. Clark Distelhorst for his kind gift of Bcl-2 plasmid. This work was supported by a research grant provided by Hubei College of Chinese Medicine (HCCM Grant No. 657616).
The authors declare that they have no competing interests.
This article does not contain any studies with human participants performed by any of the authors.
Research involving human participants and/or animals
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Adams JM, Cory S (2007) The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene 26(9):1324–1337. doi:10.1038/sj.onc.1210220 View ArticlePubMedPubMed CentralGoogle Scholar
- Andreadis C, Vahtsevanos K, Sidiras T, Thomaidis I, Antoniadis K, Mouratidou D (2003) 5-Fluorouracil and cisplatin in the treatment of advanced oral cancer. Oral Oncol 39(4):380–385View ArticlePubMedGoogle Scholar
- Camisasca DR, Honorato J, Bernardo V, da Silva LE, da Fonseca EC, de Faria PA et al (2009) Expression of Bcl-2 family proteins and associated clinicopathologic factors predict survival outcome in patients with oral squamous cell carcinoma. Oral Oncol 45(3):225–233. doi:10.1016/j.oraloncology.2008.05.021 View ArticlePubMedGoogle Scholar
- de Vicente JC, Olay S, Lequerica-Fernandez P, Sanchez-Mayoral J, Junquera LM, Fresno MF (2006) Expression of Bcl-2 but not Bax has a prognostic significance in tongue carcinoma. J Oral Pathol Med 35(3):140–145. doi:10.1111/j.1600-0714.2006.00378.x View ArticlePubMedGoogle Scholar
- Domen J, Gandy KL, Weissman IL (1998) Systemic overexpression of BCL-2 in the hematopoietic system protects transgenic mice from the consequences of lethal irradiation. Blood 91(7):2272–2282PubMedGoogle Scholar
- Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M et al (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136(5):E359–E386. doi:10.1002/ijc.29210 View ArticlePubMedGoogle Scholar
- Franke TF, Hornik CP, Segev L, Shostak GA, Sugimoto C (2003) PI3K/Akt and apoptosis: size matters. Oncogene 22(56):8983–8998. doi:10.1038/sj.onc.1207115 View ArticlePubMedGoogle Scholar
- Garnaes E, Kiss K, Andersen L, Therkildsen MH, Franzmann MB, Filtenborg-Barnkob B et al (2015) Increasing incidence of base of tongue cancers from 2000 to 2010 due to HPV: the largest demographic study of 210 Danish patients. Br J Cancer 113(1):131–134. doi:10.1038/bjc.2015.198 View ArticlePubMedPubMed CentralGoogle Scholar
- Goff DJ, Court Recart A, Sadarangani A, Chun HJ, Barrett CL, Krajewska M et al (2013) A Pan-BCL2 inhibitor renders bone-marrow-resident human leukemia stem cells sensitive to tyrosine kinase inhibition. Cell Stem Cell 12(3):316–328. doi:10.1016/j.stem.2012.12.011 View ArticlePubMedPubMed CentralGoogle Scholar
- Goldstein DP, Bachar GY, Lea J, Shrime MG, Patel RS, Gullane PJ et al (2013) Outcomes of squamous cell cancer of the oral tongue managed at the Princess Margaret Hospital. Head Neck 35(5):632–641. doi:10.1002/hed.23001 View ArticlePubMedGoogle Scholar
- Gross A, McDonnell JM, Korsmeyer SJ (1999) BCL-2 family members and the mitochondria in apoptosis. Genes Dev 13(15):1899–1911View ArticlePubMedGoogle Scholar
- Hagland H, Nikolaisen J, Hodneland LI, Gjertsen BT, Bruserud O, Tronstad KJ (2007) Targeting mitochondria in the treatment of human cancer: a coordinated attack against cancer cell energy metabolism and signalling. Expert Opin Ther Targets 11(8):1055–1069. doi:10.1517/1472818.104.22.1685 View ArticlePubMedGoogle Scholar
- Huang SH, O’Sullivan B (2013) Oral cancer: current role of radiotherapy and chemotherapy. Med Oral Patol Oral Cir Bucal 18(2):e233–e240View ArticlePubMedPubMed CentralGoogle Scholar
- Jaras M, Ebert BL (2011) Power cut: inhibiting mitochondrial translation to target leukemia. Cancer Cell 20(5):555–556. doi:10.1016/j.ccr.2011.10.028 View ArticlePubMedGoogle Scholar
- Jiang M, Milner J (2003) Bcl-2 constitutively suppresses p53-dependent apoptosis in colorectal cancer cells. Genes Dev 17(7):832–837. doi:10.1101/gad.252603 View ArticlePubMedPubMed CentralGoogle Scholar
- Khan Z, Bisen PS (2013) Oncoapoptotic signaling and deregulated target genes in cancers: special reference to oral cancer. Biochim Biophys Acta 1836(1):123–145. doi:10.1016/j.bbcan.2013.04.002 PubMedGoogle Scholar
- Kinoshita T, Yokota T, Arai K, Miyajima A (1995) Regulation of Bcl-2 expression by oncogenic Ras protein in hematopoietic cells. Oncogene 10(11):2207–2212PubMedGoogle Scholar
- Ko TK, Chuah CTH, Huang JWJ, Ng KP, Ong ST (2014) The BCL2 inhibitor ABT-199 significantly enhances imatinib-induced cell death in chronic myeloid leukemia progenitors. Oncotarget 5(19):9033–9038View ArticlePubMedPubMed CentralGoogle Scholar
- Lagadinou ED, Sach A, Callahan K, Rossi RM, Neering SJ, Minhajuddin M et al (2013) BCL-2 inhibition targets oxidative phosphorylation and selectively eradicates quiescent human leukemia stem cells. Cell Stem Cell 12(3):329–341. doi:10.1016/j.stem.2012.12.013 View ArticlePubMedPubMed CentralGoogle Scholar
- Mallick S, Patil R, Gyanchandani R, Pawar S, Palve V, Kannan S et al (2009) Human oral cancers have altered expression of Bcl-2 family members and increased expression of the anti-apoptotic splice variant of Mcl-1. J Pathol 217(3):398–407. doi:10.1002/path.2459 View ArticlePubMedGoogle Scholar
- Mayevsky A (2009) Mitochondrial function and energy metabolism in cancer cells: past overview and future perspectives. Mitochondrion 9(3):165–179. doi:10.1016/j.mito.2009.01.009 View ArticlePubMedGoogle Scholar
- Murugan AK, Munirajan AK, Tsuchida N (2012) Ras oncogenes in oral cancer: the past 20 years. Oral Oncol 48(5):383–392. doi:10.1016/j.oraloncology.2011.12.006 View ArticlePubMedGoogle Scholar
- Popovic B, Jekic B, Novakovic I, Lukovic LJ, Tepavcevic Z, Jurisic V et al (2007) Bcl-2 expression in oral squamous cell carcinoma. Ann N Y Acad Sci 1095:19–25. doi:10.1196/annals.1397.003 ADSView ArticlePubMedGoogle Scholar
- Shiboski CH, Schmidt BL, Jordan RC (2005) Tongue and tonsil carcinoma: increasing trends in the U.S. population ages 20–44 years. Cancer 103(9):1843–1849. doi:10.1002/cncr.20998 View ArticlePubMedGoogle Scholar
- Song LB, Zeng MS, Liao WT, Zhang L, Mo HY, Liu WL et al (2006) Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells. Cancer Res 66(12):6225–6232. doi:10.1158/0008-5472.CAN-06-0094 View ArticlePubMedGoogle Scholar
- Souers AJ, Leverson JD, Boghaert ER, Ackler SL, Catron ND, Chen J et al (2013) ABT-199, a potent and selective BCL-2 inhibitor, achieves antitumor activity while sparing platelets. Nat Med 19(2):202–208. doi:10.1038/nm.3048 View ArticlePubMedGoogle Scholar
- Tan WJ, Chia CS, Tan HK, Soo KC, Iyer NG (2012) Prognostic significance of invasion depth in oral tongue squamous cell carcinoma. ORL 74(5):264–270. doi:10.1159/000343796 View ArticlePubMedGoogle Scholar
- Vander Heiden MG (2011) Targeting cancer metabolism: a therapeutic window opens. Nat Rev Drug Discov 10(9):671–684. doi:10.1038/nrd3504 View ArticlePubMedGoogle Scholar
- Varum S, Rodrigues AS, Moura MB, Momcilovic O, Easley CA, Ramalho-Santos J et al (2011) Energy metabolism in human pluripotent stem cells and their differentiated counterparts. PLoS One 6(6):e20914. doi:10.1371/journal.pone.0020914 ADSView ArticlePubMedPubMed CentralGoogle Scholar
- Wang NS, Unkila MT, Reineks EZ, Distelhorst CW (2001) Transient expression of wild-type or mitochondrially targeted Bcl-2 induces apoptosis, whereas transient expression of endoplasmic reticulum-targeted Bcl-2 is protective against Bax-induced cell death. J Biol Chem 276(47):44117–44128. doi:10.1074/jbc.M101958200 View ArticlePubMedGoogle Scholar
- Wen H, Chen Y, Hu Z, Mo Q, Tang J, Sun C (2014) Decreased expression of BATF2 is significantly associated with poor prognosis in oral tongue squamous cell carcinoma. Oncol Rep 31(1):169–174. doi:10.3892/or.2013.2863 PubMedGoogle Scholar
- Zhang B, Liu M, Tang HK, Ma HB, Wang C, Chen X et al (2012) The expression and significance of MRP1, LRP, TOPOIIbeta, and BCL2 in tongue squamous cell carcinoma. J Oral Pathol Med 41(2):141–148. doi:10.1111/j.1600-0714.2011.01066.x View ArticlePubMedGoogle Scholar