Fig. 4

Bcl-2 inhibitor impairs mitochondrial functions in OTSCCs. a ABT-199 decreases mitochondrial membrane potential in SCC-25, SCC-9, CAL27 and Tca3188 cells. Cells were stained with JC-1 dye prior to flow cytometry. b ABT-199 dose-dependently inhibits mitochondrial respiration. OCR was measured using a Seahorse XF24 extracellular flux analyser in the absence of compound injection. ABT-199 increases ROS (c) and decreases ATP (d) levels in OTSCCs. Cells were treated with ABT-199 for 24 h prior to mitochondrial function assays. Data were presented as mean ± SD. *p < 0.05