Membrane property and biofunction of phospholiposome incorporated with anomeric galactolipids
© The Author(s). 2016
Received: 13 January 2016
Accepted: 26 April 2016
Published: 17 May 2016
There has been increasing interest in the construction of liposomes containing a targeting reagent for target-specific drug delivery. Glycoconjugates that can be recognized by transmembrane glycoprotein receptors have been extensively used to form glyco-liposomal drug carriers. However, the impact of anomerism, which is a common identity of natural glycoconjugates, on the glyco-liposomal properties has been hardly probed in previous studies. Here we investigate the liposomal properties of phospholipid incorporated with a pair of anomeric galactolipids. The anomeric galacto-liposomes are characterized and their membrane fluidity, thermo-stability, DNA condensation efficiency and fluorescence leakage are comparatively tested. The in vitro cellular internalization effect of the galacto-liposomes is also demonstrated. This study suggests that anomerism might give distinct impact on the membrane properties and even biofunctions of glyco-liposomes.
Liposome has the similar structure to biomembrane, such as cell membrane, and is always used as substitute in research of biomembrane. It is easy to believe that the properties of membranes should give large influence on their biofunctions. For example, lower thermal stability might increase membrane permeability and release drug quickly (Yang et al. 2014). Positive charged liposomal surface might promote interaction with cells (Rädler et al. 1997). And even, the membrane fusion should be determined partly by properties of bilayer membranes.
In the drug or DNA delivery system, liposomes can effectively protect cargoes from being biodegraded in vivo before reaching a target tissue. However, the drug delivering efficiency of commercial liposomes is usually compromised due to the lack of a ‘warhead’ that effectively directs them to the target. In recent years, construction of glycolipid (a saccharide covalently linked to a lipid)-incorporated liposomes (Jayaraman et al. 2013; Ueno et al. 2007; Róg et al. 2007; Ramezani et al. 2009; Stimac et al. 2012; Yin et al. 2013) has been of increasing interest since the glycosyl group of which can be specifically recognized by a transmembrane glycoprotein receptor of a target tissue (Weis et al. 1998; Marth and Grewal 2008). The recognition may then facilitate the endocytosis and/or fusion of the liposomes by the host cells, releasing an encapsulated therapeutic agent. Among them, galactoliposomes that can be recognized by the asialoglycoprotein receptors (ASGPR) expressed by the hepatocyte (Rigopoulou et al. 2012) are the most extensively developed, and are considered to be promising non-viral vectors for target-specific treatment of hepatic diseases (Sliedregt et al. 1999; Zhao et al. 2011; Symens et al. 2012; Hu et al. 2013; Jain et al. 2012). Monosaccharides are linked to each other or with an aglycon in either the α- or β-configuration (at the anomeric carbon) to form cell-surface ‘antennas’, such as glycoproteins or glycolipids, that transmit signals between cells (Varki 1993; Bertozzi and Kiessling 2001; Seeberger 2005; Hart and Copeland 2010). These anomeric counterparts may, however, possess different physiochemical attributes, thereby leading to a diversification of biological function. In addition, some synthetic anomeric glycolipids have shown distinct pharmacological properties (He et al. 2011). Despite the fruitful results obtained in the investigation of glycolipid-based liposomal transfection (Ueno et al. 2007), to our knowledge, investigations on the properties of anomeric galacto-liposomes have been elusive.
Preparation of liposome
5.0 mg of DPPE (99 %, Lipoid, Germany) and 1.0 mg of cationic cholesterol (Sigma, USA) (molar ratio ≈ 3/1) were dissolved in 5 mL of chloroform, and 2.5 mg of galactolipid was dissolved in 5 mL of dichloromethane containing minute quantity of methyl alcohol. Then the two solutions were mixed with different molar ratios. The organic solvents were removed by a rotary evaporator for 45 min at 37 °C and another 1 h under vacuum at room temperature. The lipid film was hydrated with ultrapure water (for characterization experiments) or Tris–HCl buffer solution (10 mM, pH 7.4, for biology experiments), and the suspension was sonicated in a bath sonicator under 65 °C for 30 min. The suspension was then extruded (11 times) through a polycarbonate membrane with a pore diameter of 200 nm (Whatman, UK) by using LiposoFast (LF-1, Avestin, Canada). The particle sizes were about 150 ± 10 nm measured by a dynamic light scattering (Zetasizer Nano S, Malvern, UK). Their phase transition temperatures were determined on a differential scanning calorimetry DSC (Setaram DSC III, USA) at a scan rate of 1 °C/min covering a temperature range of 25–70 °C. For TEM measurement, the samples were dropped onto a copper net and the morphologies of liposomes were recorded with JEOL JEM-1400.
Membrane fluidity of liposome
Load and release of fluorescein
Liposomes were prepared as mentioned above except that the lipid membrane was hydrated with a fluorescein (400 μM) contained Tris–HCl buffer solution (10 mM, pH 7.4). The not-incorporated fluorescein molecules were removed by gel permeation chromatography on a Sephadex G50 resin column, which was preconditioned with PBS (pH 7.4). The fluorescein left in gel column was measured for calculating the loading amount in gel layer. The weight ratio of incorporated fluorescein to lipid was calculated to be 1/100 (g/g). 2 mL of fluorescein loaded liposome was sealed in a dialysis tube (MWCO 3500), which was placed in 30 mL of phosphate buffered saline (PBS, pH 7.4). The fluorescein leakages were investigated at 37 °C by using a spectrfluorometer (F-4500, Hitachi, Japan). The emission and excitation wavelengths were 517 nm and 495 nm. Complete release of fluorescein was obtained from controlling ethanol-treated liposome. The accumulated leakage percentage was then determined by the FL intensity relative to 100 % dequenching.
Preparation of plasmid DNA
Plasmid DNA pIRES2-EGFP (pDNA) was purchased from BD Biosciences Clontech, USA. EndoFree Plasmid Maxi Kit (Qiagen, Germany) was used to prepare high-quality endotoxin free plasmid. Briefly, pDNA was transferred into competent cell Escherichia coli DH5α and streaked on kanamycin plate. A single colony was picked from the freshly selective plate and inoculated a starter culture of 3 mL Luria–Bertani medium containing 50 μg/mL kanamycin for 6 h at 37 °C with 300 rpm shaking. The starter culture was diluted at 1/500 into 200 mL LB medium containing 50 μg/mL kanamycin, and grew at 37 °C for 12 h with vigorous shaking (300 rpm). After inoculation, the bacterial cells were harvested by centrifugation at 4000×g for 15 min at 4 °C. After being re-suspended, lyses and neutralization, the pDNA was released from the lysate. Being recovered and purified with QIAGEN-tips, the pDNA was eventually resolved in 500 μL endotoxin-free Buffer TE at a final concentration of 1.2 μg/μL.
Evaluation of pDNA condensation efficiency of liposome
pDNA was diluted with Tris–HCl buffer (pH 7.4) and mixed with liposome suspension at a terminal final concentration of 3.6 μg/mL. The mixture was kept for 30 min at room temperature before further experiment. The amount of pDNA protected from intercalation of EtBr by liposome was evaluated by ethidium intercalation assay (EtBr was excited at 520 nm to produce a FL emission at 595 nm). The pDNA composition efficiency was obtained through dividing the relative FL intensity of EtBr added to pDNA encapsulated in liposome by the maximum FL intensity of which added to free pDNA.
In vitro cellular internalization effect of liposome to Huh7 cells
Rhodamine 6G loaded liposomes were prepared as mentioned above. Hepatocarcinoma cell line Huh7 was recovered from liquid nitrogen using DMEM (Gbico, USA) culture media with 10 % FBS and P-S antibiotics, and passed 2–3 generations before use. Cells were placed in a volume of 500 μL growth medium without antibiotics per well in a 24-well plate 1 day before transfection. The cells would be 90–95 % confluent at the time of transfection. The liposome suspension (30 μg/mL, 200 μL/per well) was transfected to 24-well plate containing 90–95 % confluent fresh cells and was rinsed by growth medium after 15 or 30 min incubation individually. Then the samples were imaged under a FL microscopy.
Characterization of galacto-liposomes
Isomerism has been suggested to impact the LB monolayer property of glycolipids as well as the cell uptake of glycopolymer-containing nanoparticles (Liu et al. 2015). We have recently determined that anomerism, which is a common identity of nearly all glycoconjugates, could influence largely the bioactivity of glycolipids (He et al. 2011). Despite the extensive efforts in the construction of glyco-liposomes, investigations as regards the impact of anomerism on glyco-liposomes have been elusive.
Here we used a pair of anomeric galactolipids with a 16-carbon lipid chain, synthesized in a previous study (He et al. 2011), to test their liposomal properties while imbedded in a phospholipid-based liposome (Fig. 2). Liposomes that consist of pure DPPE (LipoDPPE), DPPE embedded with α-galactolipid 1 (Lipo1) or DPPE embedded with β-galactolipid 2 (Lipo2) of a similar particle size were fabricated. The mixing ratio was below 30 % as a previous study has suggested (Ueno et al. 2007) that further over-mixing would compromise the stability of glyco-liposomes.
The membrane fluidity of liposomes is an important parameter, which can influence liposomal biofunctions such as drug encapsulation and drug release. Indeed, higher membrane fluidity can result in a quicker drug leakage or releasing. The 1/P values, which are proportional to the membrane fluidity of the liposome, are calculated from the DPH FL polarizations (Fig. 5b) (Treichel et al. 1994). We observed that the 1/P values of LipoDPPE increased with increasing galactolipid, while the 1/P value of Lipo1 was lower than that of Lipo2.
The drug leakage of liposomes
DNA condensation efficiency
The DNA condensation efficiencies of the pure liposome and galacto-liposome were judged through the FL intensity of EtBr. When DNA molecules were encapsulated or condensed, EtBr cannot insert into DNA base pairs and then fluoresce. A plasmid DNA that encodes the internal ribosome entry site and the enhanced green fluorescent protein (pIRES2-EGFP) was employed.
In vitro cellular internalization
As galactolipid was incorporated into liposomes, the influence of the galactolipid on liposomes was characteries by DSC, FL polarization and drug leakage experiment. As shown in Fig. 5a, obviously, the imbedding of 2 has a larger impact on the stability of the liposomal property than 1. It is known that imbedding lipids with unsaturated carbon bond or with shorter carbon chain should decrease T m values and then thermo-stability. Here, being imbedded into the DPPE liposome, the anomeric galactolipids could probably have different interactions with DPPE which results from their small configurational difference as mentioned in a previous paper (Liu et al. 2015). The observation that the T m of Lipo1 is higher than Lipo2 (Fig. 5a) might be caused by a stronger intermolecular interaction of the α-galacto-liposome than the β-counterpart, leading to the increased thermal stability.
From Fig. 5b, the 1/P values of LipoDPPE were found to increase with content of galactolipid, suggesting that the DPPE molecules are disturbed by addition of the galactolipid molecules. And Lipo1 had lower 1/P value than Lipo2, which is also thought to be related to the interaction between DPPE and the anomeric galactolipids. A stronger interacton might hamper the motion of the moleclues in the bilayer.
For the fluorescein leakage experiment shown in Fig. 5b, their accumulated leakages increased with content of galactolipid and 1/P value. The accumulated leakages of Lipo2 were slightly higher than those of Lipo1, indicating the less stable membrane of the former. As a drug carrier, high drug leakage is thought to increase side-effect of drug during cycling in body. However, too stable liposome might have another problem, that is, might tend to hardly release even if the carrier gets to the sick site. So it is necessary to study on drug release system which could release smartly and controllably.
From these results, we observed that, with the increase of the molar fraction of galactolipids, the thermal stability decreased and the fluidity of the liposome membrane increased. The higher membrane fluidity may account for its lower thermal stability. This is because the two properties might both relate directly to the motion of lipid carbon chain.
The ability of these liposomes to condense DNA was also investigated. Although it has been suggested that the charge amount, fluidity of membrane and alkyl chain length of lipsome had impact on the DNA condensation and transfection efficiency, the exact mechanisms are not known (Lentz 1993). It is easy to think that the DNA condensation efficiency should firstly depend on the Zeta-potential and size of the liposome, which have been unified in this experiment. And secondly, the micro-property of liposomal membrane, including membrane fluidity, intermolecular interaction, molecular density, etc., should be concerned. According to the discussion mentioned above, we focus on the first two. The difference in DNA condensation between Lipo1 and Lipo2 could probably be ascribed to the slightly higher fluidity and weaker molecular interaction of the β- than the α-galacto-liposome (Song et al. 2012), which is unfavourable to condense the DNA strand more efficiently. We deduced that the liposomal membrane with higher fluidity (Lipo2) might hamper the process of DNA adsorption and condensation, leading to decreased DNA condensation efficiency.
As for the cellular internalization ability of these liposomes, within the short incubation with cells, there was very little amount of rhodamine released from liposomes. So it could be thought that there was very little free rhodamine could enter into cells through permeabilization. The intensity of FL was used to judge the amount of internalized liposomes. It was found that the difference between the results of Lipo1 and Lipo2 was not significant from the FL images. However, both anomeric galacto-liposomes showed better internalization ability than the pure DPPE liposome, suggesting that the presence of the galactoside warhead might give an improvement in the function of cellular internalization.
From the quantitative results of internalization, it was found that increase the ratio of galactolipid did not improve the internalization linearly, suggesting this bioprocess of cellular internalization should be very complex. But we could confirm that it was impacted seriously by membrane properties. An appropriate fluidity of liposomal membrane should be necessary. It was indicated a lower containing ratio of galactolipid seemed to get a less drug leakage and a better internalization effect.
We note that obvious difference was observed between the anomeric counterparts (Lipo1 and Lipo2), suggesting that further sophisticated experiments are needed to elaborate such difference. However, the above-shown cellular data clearly demonstrated the advantage of using Lipo1 for preparing hepatoma cell targeted drug carriers.
As what discussed above, using a pair of anomeric galactolipids we showed that their liposomal properties and biofunctions, while incorporated with a DPPE based liposome, were different as a result of their small configurational difference between α- and β-galacolipid. Galacto-liposomes (Lipo1 and Lipo2) showed decreased thermal stability and increased membrnane fluidity, leading to the better DNA condensation efficiency probably due to stronger ability to bind and condense DNA chains. And also the all galacto-liposomes, especially Lipo1, showed better internalization ability than the pure DPPE liposome. We suggest that the elaboration of the structural and functional distinction between isomeric glyco-liposomes should be an important task to address in the field of targeted drug delivery, shedding light on the better understanding of the endocytosis and membrane fusion of these vesicles on the cellular level.
The manuscript was conceived and designed by XSH and LHL. LDY and ZJQ performed acquisition and analysis of data. All authors were involved in revising the manuscript. All authors read and approved the final manuscript.
The galactolipid was kindly provided by Prof. Xiao-Peng He. This work is supported by the National Natural Science Foundation of China (21276074, 91334203) and the Fundamental Research Funds for the Central Universities.
The authors declare that they have no competing interests.
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- Bertozzi CR, Kiessling LL (2001) Chemical glycobiology. Science 291(5512):2357–2364View ArticleGoogle Scholar
- Hart GW, Copeland RJ (2010) Glycomics hits the big time. Cell 143(5):672–676View ArticleGoogle Scholar
- He X-P, Xu X, Zhang H-L, Chen G-R, Xu S, Liu H (2011) Disclosing the distinct interfacial behaviors of structurally and configurationally diverse triazologlycolipids. Carbohydr Res 346(11):1320–1326View ArticleGoogle Scholar
- Hu Y, Haynes MT, Wang Y, Liu F, Huang L (2013) A highly efficient synthetic vector: nonhydrodynamic delivery of DNA to hepatocyte nuclei in vivo. ACS Nano 7(6):5376–5384View ArticleGoogle Scholar
- Jain K, Kesharwani P, Gupta U, Jain NK (2012) A review of glycosylated carriers for drug delivery. Biomaterials 33(16):4166–4186View ArticleGoogle Scholar
- Jayaraman N, Maiti K, Naresh K (2013) Multivalent glycoliposomes and micelles to study carbohydrate–protein and carbohydrate–carbohydrate interactions. Chem Soc Rev 42(11):4640–4656View ArticleGoogle Scholar
- Kobayashi T, Ishida T, Okada Y, Ise S, Harashima H, Kiwada H (2007) Effect of transferrin receptor-targeted liposomal doxorubicin in P-glycoprotein-mediated drug resistant tumor cells. Int J Pharm 329(1–2):94–102View ArticleGoogle Scholar
- Lentz BR (1993) Use of fluorescent probes to monitor molecular order and motions within liposome bilayers. Chem Phys Lipids 64(1):99–116View ArticleGoogle Scholar
- Liu D, Wang W, He X, Chen G, Xu S, Liu H (2015) Anomerism differentiates the interfacial property of galactolipid-doped phospholipid monolayers. Issues Biol Sci Pharm Res 3(8):78–85Google Scholar
- Marth JD, Grewal PK (2008) Mammalian glycosylation in immunity. Nat Rev Immunol 8(11):874–887View ArticleGoogle Scholar
- Rädler JO, Koltover I, Salditt T, Safinya CR (1997) Structure of DNA–cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes. Science 275(5301):810–814View ArticleGoogle Scholar
- Ramezani M, Khoshhamdam M, Dehshahri A, Malaekeh-Nikouei B (2009) The influence of size, lipid composition and bilayer fluidity of cationic liposomes on the transfection efficiency of nanolipoplexes. Colloids Surf B 72(1):1–5View ArticleGoogle Scholar
- Rigopoulou EI, Roggenbuck D, Smyk DS, Liaskos C, Mytilinaiou MG, Feist E, Conrad K, Bogdanos DP (2012) Asialoglycoprotein receptor (ASGPR) as target autoantigen in liver autoimmunity: lost and found. Autoimmun Rev 12(2):260–269View ArticleGoogle Scholar
- Róg T, Vattulainen I, Bunker A, Karttunen M (2007) Glycolipid membranes through atomistic simulations: effect of glucose and galactose head groups on lipid bilayer properties. J Phys Chem B 111(34):10146–10154View ArticleGoogle Scholar
- Seddon JM, Cevc G, Marsh D (1983) Calorimetric studies of the gel-fluid (L.beta.–L.alpha.) and lamellar-inverted hexagonal (L.alpha.-HII) phase transitions in dialkyl- and diacylphosphatidylethanolamines. Biochemistry 22(5):1280–1289View ArticleGoogle Scholar
- Seeberger PH (2005) Exploring life’s sweet spot. Nature 437(7063):1239View ArticleGoogle Scholar
- Sliedregt LAJM, Rensen PCN, Rump ET, van Santbrink PJ, Bijsterbosch MK, Valentijn ARPM, van der Marel GA, van Boom JH, van Berkel TJC, Biessen EAL (1999) Design and synthesis of novel amphiphilic dendritic galactosides for selective targeting of liposomes to the hepatic asialoglycoprotein receptor. J Med Chem 42(4):609–618View ArticleGoogle Scholar
- Song S-X, Wu M-L, He X-P, Zhou Y-B, Sheng L, Li J, Chen G-R (2012) The anomeric mixture of some O-galactolipid derivatives is more toxic against cancer cells than either anomer alone. Bioorg Med Chem Lett 22(5):2030–2032View ArticleGoogle Scholar
- Stimac A, Segota S, Dutour Sikiric M, Ribic R, Frkanec L, Svetlicic V, Tomic S, Vranesic B, Frkanec R (2012) Surface modified liposomes by mannosylated conjugates anchored via the adamantyl moiety in the lipid bilayer. Biochim Biophys Acta 1818(9):2252–2259View ArticleGoogle Scholar
- Sun P, He Y, Lin M, Zhao Y, Ding Y, Chen G, Jiang M (2014) Glyco-regioisomerism effect on lectin-binding and cell-uptake pathway of glycopolymer-containing nanoparticles. ACS Macro Lett 3(1):96–101View ArticleGoogle Scholar
- Symens N, Méndez-Ardoy A, Díaz-Moscoso A, Sánchez-Fernández E, Remaut K, Demeester J, Fernández JMG, De Smedt SC, Rejman J (2012) Efficient transfection of hepatocytes mediated by mRNA complexed to galactosylated cyclodextrins. Bioconjug Chem 23(6):1276–1289View ArticleGoogle Scholar
- Treichel U, Meyer zum Büschenfelde K-H, Stockert RJ, Poralla T, Gerken G (1994) The asialoglycoprotein receptor mediates hepatic binding and uptake of natural hepatitis B virus particles derived from viraemic carriers. J Gen Virol 75(11):3021–3029View ArticleGoogle Scholar
- Ueno Y, Hirashima N, Inoh Y, Furuno T, Nakanishi M (2007) Characterization of biosurfactant-containing liposomes and their efficiency for gene transfection. Biol Pharm Bull 30(1):169–172View ArticleGoogle Scholar
- Varki A (1993) Biological roles of oligosaccharides: all of the theories are correct. Glycobiology 3(2):97–130View ArticleGoogle Scholar
- Weis WI, Taylor ME, Drickamer K (1998) The C-type lectin superfamily in the immune system. Immunol Rev 163(1):19–34View ArticleGoogle Scholar
- Yang Y, Yang Y, Xie X, Cai X, Zhang H, Gong W, Wang Z, Mei X (2014) PEGylated liposomes with NGR ligand and heat-activable cell-penetrating peptide–doxorubicin conjugate for tumor-specific therapy. Biomaterials 35(14):4368–4381View ArticleGoogle Scholar
- Yin L, Song Z, Kim KH, Zheng N, Gabrielson NP, Cheng J (2013) Non-viral gene delivery via membrane-penetrating, mannose-targeting supramolecular self-assembled nanocomplexes. Adv Mater 25(22):3063–3070View ArticleGoogle Scholar
- Zhao Q, Chen W, Chen Y, Zhang L, Zhang J, Zhang Z (2011) Self-assembled virus-like particles from rotavirus structural protein VP6 for targeted drug delivery. Bioconjug Chem 22(3):346–352View ArticleGoogle Scholar