- Open Access
Intracellular complexes of the early-onset torsion dystonia-associated AAA+ ATPase TorsinA
© Li et al.; licensee Springer. 2014
- Received: 21 October 2014
- Accepted: 9 December 2014
- Published: 16 December 2014
A single GAG codon deletion in the gene encoding torsinA is linked to most cases of early-onset torsion dystonia. TorsinA is an ER-localized membrane-associated ATPase from the AAA+ superfamily with an unknown biological function. We investigated the formation of oligomeric complexes of torsinA in cultured mammalian cells and found that wild type torsinA associates into a complex with a molecular weight consistent with that of a homohexamer. Interestingly, the dystonia-linked variant torsinAΔE displayed a reduced propensity to form the oligomers compared to the wild type protein. We also discovered that the deletion of the N-terminal membrane-associating region of torsinA abolished oligomer formation. Our results demonstrate that the dystonia-linked mutation in the torsinA gene produces a protein variant that is deficient in maintaining its oligomeric state and suggest that ER membrane association is required to stabilize the torsinA complex.
- Early-onset dystonia, TorsinA
- AAA+ ATPase
- Protein association
Early-onset torsion dystonia (EOTD) is the most common and severe form of primary dystonia, a neurological disorder that manifests as uncontrollable movements and abnormal body postures. Most cases of EOTD are associated with a deletion of a single GAG codon in the DYT1 gene. As a result, a single glutamic acid residue is absent in the EE pair located in the C-terminal region of torsinA (Ozelius et al. 1997). TorsinA is a putative member of the AAA+ superfamily of A TPases a ssociated with different a ctivities (Neuwald et al. 1999). The torsinA mRNA is widely expressed in various human tissues, including the central nervous system, but the biological role of torsinA is not completely clear (reviewed in (Tanabe et al. 2009; Zolkiewski and Wu 2011)). AAA+ ATPases are energy-driven “molecular machines”, which remodel the conformation of macromolecules and disassemble macromolecular complexes (Hanson and Whiteheart 2005). Proteins from the AAA+ family form ring-shaped hexameric complexes, which enclose their substrate molecules. Hexamer formation is essential for the activity of AAA+ ATPases (Barnett et al. 2000). Numerous torsinA partners have been identified and the association with some of these is compromised when the mutant gene product is expressed (Naismith et al. 2009; Zolkiewski and Wu 2011). However, the identity of a torsinA substrate that is critical for its cellular activity is unknown and, more fundamentally, whether torsinA even forms a hexamer in cells has not been fully established. Moreover, it is unclear how the glutamate deletion affects these biochemical properties and in turn, which defect(s) associated with the mutant protein are linked to EOTD.
The torsinA sequence contains an N-terminal ER-targeting signal peptide that is cleaved upon import into the ER lumen, producing the mature 36-kDa form of the protein (Liu et al. 2003). The signal sequence is followed by a 20-residue-long hydrophobic segment that is responsible for membrane association (Liu et al. 2003) and ER retention (Vander Heyden et al. 2011). The AAA+ module of torsinA is located downstream of the membrane-binding domain and contains a non-canonical ATP-binding Walker-A motif (Nagy et al. 2009; Zolkiewski and Wu 2011) and six cysteines that are absent from other AAA+ ATPases (Zhu et al. 2008; Zolkiewski and Wu 2011). The site of the dystonia-linked glutamate deletion (E302/E303) is located within the C-terminal AAA+ subdomain, which supports oligomerization of other AAA+ ATPases (Barnett et al. 2000). Studies with purified recombinant torsinA revealed either a monomeric protein (Kustedjo et al. 2003; Zhu et al. 2008) or a spectrum of high-molecular weight particles (Zhao et al. 2013). In contrast, torsinA assemblies ranging from monomers and dimers to hexamers were detected in lysates from mammalian cells (Kustedjo et al. 2000; Gordon and Gonzalez-Alegre 2008; Vander Heyden et al. 2009; Jungwirth et al. 2010). How these assemblies are affected by the disease-causing mutation or the hydrophobic membrane anchor has not yet been established.
To this end, we investigated the size of human torsinA complexes after isolation from cultured mammalian cells. We found that the main oligomeric species is consistent with the formation of torsinA hexamers, but this structure becomes less stable when the dystonia-linked protein variant is expressed. We also found that the membrane-bound hydrophobic segment stabilizes the torsinA oligomer. These data add fundamental new insights to our understanding of torsinA structure and suggest why the loss of a single amino acid can exhibit profound cellular effects.
To corroborate these data, we next investigated the oligomeric state of torsinAΔ40 in mammalian cell lysates (Figure 2C). In contrast to the full-length protein (WT), the torsinAΔ40 and torsinAΔ40ΔE variants again failed to form oligomeric species in BN-PAGE. This result is in accordance with the properties of purified torsinAΔ40 (Figure 2B) and indicates that the 20 residue-long N-terminal hydrophobic segment is essential to stabilize torsinA complexes. Two mechanisms can be proposed to account for this result. First, the hydrophobic segment may directly participate in either self-association or hetero-association with another protein’s membrane-embedded domain. Second, the membrane association of torsinA and its retention in the ER lumen may increase the likelihood of forming the homo- or heterooligomers. Recently, hetero-hexamers of torsinA and LAP1 were reconstituted with purified proteins (Sosa et al. 2014). Since LAP1 is a transmembrane protein, its interaction with torsinA in the cell might be efficient only if torsinA is also targeted to the ER membrane by its N-terminal hydrophobic segment. Future efforts will be directed to address this hypothesis.
In summary, we found that torsinA forms a discrete high-molecular weight complex in mammalian cells. However, the complex is destabilized by the dystonia-linked mutation, but is stabilized by the membrane anchor. Establishing a link between defects in torsinAΔE oligomerization and EOTD will be an important focus of future research efforts.
Plasmids, antibodies, and reagents
DNA constructs containing the human torsinA sequence in pcDNA3 vector were described before (Liu et al. 2003) and anti-torsin antibodies were obtained as described (Zacchi et al. 2014). Sweet potato β-amylase was from Sigma. Native electrophoresis protein standards were from Invitrogen/Life Technologies.
HEK293 cells were maintained in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (BioWhittaker) at 37°C in the presence of 5% CO2. CHO-K1 cells (ATCC) were maintained in F-12 K medium (Invitrogen) supplemented with 10% fetal bovine serum. Cells were transfected with pcDNA3 expression vectors containing the torsinA variants using FuGene 6 transfection reagent (Roche) according to the manufacturer’s instructions. Stably transfected cells were selected in the presence of 1 mg/mL G418 (Invitrogen).
BN-PAGE is a native gel electrophoresis technique, where the Coomassie Brilliant Blue dye binds to membrane protein complexes and provides the electric charge for the electrophoretic separation. BN-PAGE was carried out as previously described (Wittig et al. 2006; Vander Heyden et al. 2009; Jungwirth et al. 2010). Briefly, ~90% confluent cells were collected and solubilized in a lysis buffer (50 mM imidazole pH 7.0, 50 mM NaCl, 2 mM 6-aminohexanoic acid, 4 mM MgCl2, 2 mM EDTA, 2 mM ATP, 1 mM PMSF, and 0.25% dodecylmaltoside) for 15 min in 4°C, and centrifuged twice for 15 min at 15,000 rpm in IEC Micromax benchtop centrifuge. The supernatant supplemented with 0.0625% Coomassie blue G-250 and 5% glycerol was loaded onto a 9% polyacrylamide gel. Following electrophoresis, separated proteins were transferred onto PVDF membrane and subjected to immunoblotting using anti-torsinA antibodies, followed by horseradish peroxidase-conjugated anti-rabbit IgG antibodies (Southern Biotechnology). Signal detection was performed with WestPico chemiluminescence kit (Pierce).
The S2 cell line stably transfected with a torsinAΔ40 expression plasmid containing the BiP signal sequence followed by an N-terminal His-tag was produced as previously described (Liu et al. 2003). Cells were grown at 23°C in S2 medium (Invitrogen) supplemented with 0.5% DMSO when the density reached 107 cells/ml and protein expression was induced after 24 h with 0.7 mM CuSO4. The cells were separated from the culture medium by centrifugation 6 days post induction. The medium was filtered through a 0.45 μm membrane and loaded onto a 2.5-ml Chelating Sepharose column (GE Healthcare). Proteins were eluted with an imidazole step concentration gradient. Fractions containing torsinAΔ40 (eluted at 10–50 mM imidazole) were pooled, concentrated on Centriplus YM-10 (Millipore), and dialyzed in 50 mM Tris–HCl pH 7.5, 100 mM NaCl, 20 mM MgCl2, and 10% glycerol.
Circular dichroism spectroscopy
CD spectra were measured with a Jasco J-720 spectrometer using a 0.01-cm cylindrical cuvette at room temperature.
Gel filtration analysis was performed at room temperature with a Shimadzu HPLC. TorsinAΔ40 samples (20 μl, ~0.2 mg/ml) were analyzed at 0.04 ml/min on a Superdex 200 PC 3.2/30 column (GE Healthcare) equilibrated in 50 mM Tris/HCl pH 7.5, 0.2 M KCl, 20 mM MgCl2 without nucleotides or with 2 mM ATP or ADP.
This research was supported by a grant (to JLB and MZ) and a postdoctoral fellowship (to LFZ) from the Dystonia Medical Research Foundation and by grant GM75061 from the National Institutes of Health to JLB. This is contribution 15-105-J from the Kansas Agricultural Experiment Station. Publication of this article was funded in part by the Kansas State University Open Access Publishing Fund.
- Akoev V, Gogol EP, Barnett ME, Zolkiewski M: Nucleotide-induced switch in oligomerization of the AAA+ ATPase ClpB. Protein Sci 2004, 13: 567-574. 10.1110/ps.03422604View ArticleGoogle Scholar
- Barnett ME, Zolkiewska A, Zolkiewski M: Structure and activity of ClpB from escherichia coli. Role of the amino-and -carboxyl-terminal domains. J Biol Chem 2000, 275: 37565-37571. 10.1074/jbc.M005211200View ArticleGoogle Scholar
- Goodchild RE, Dauer WT: Mislocalization to the nuclear envelope: an effect of the dystonia-causing torsinA mutation. Proc Natl Acad Sci U S A 2004, 101: 847-852. 10.1073/pnas.0304375101View ArticleGoogle Scholar
- Goodchild RE, Dauer WT: The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein. J Cell Biol 2005, 168: 855-862. 10.1083/jcb.200411026View ArticleGoogle Scholar
- Gordon KL, Gonzalez-Alegre P: Consequences of the DYT1 mutation on torsinA oligomerization and degradation. Neuroscience 2008, 157: 588-595. 10.1016/j.neuroscience.2008.09.028View ArticleGoogle Scholar
- Hanson PI, Whiteheart SW: AAA+ proteins: have engine, will work. Nat Rev Mol Cell Biol 2005, 6: 519-529. 10.1038/nrm1684View ArticleGoogle Scholar
- Jungwirth M, Dear ML, Brown P, Holbrook K, Goodchild R: Relative tissue expression of homologous torsinB correlates with the neuronal specific importance of DYT1 dystonia-associated torsinA. Hum Mol Genet 2010, 19: 888-900. 10.1093/hmg/ddp557View ArticleGoogle Scholar
- Kustedjo K, Bracey MH, Cravatt BF: Torsin A and its torsion dystonia-associated mutant forms are lumenal glycoproteins that exhibit distinct subcellular localizations. J Biol Chem 2000, 275: 27933-27939.Google Scholar
- Kustedjo K, Deechongkit S, Kelly JW, Cravatt BF: Recombinant expression, purification, and comparative characterization of torsinA and its torsion dystonia-associated variant delta E-torsinA. Biochemistry 2003, 42: 15333-15341. 10.1021/bi0349569View ArticleGoogle Scholar
- Liu Z, Zolkiewska A, Zolkiewski M: Characterization of human torsinA and its dystonia-associated mutant form. Biochem J 2003, 374: 117-122. 10.1042/BJ20030258View ArticleGoogle Scholar
- Nagy M, Wu HC, Liu Z, Kedzierska-Mieszkowska S, Zolkiewski M: Walker-A threonine couples nucleotide occupancy with the chaperone activity of the AAA+ ATPase ClpB. Protein Sci 2009, 18: 287-293. 10.1002/pro.36View ArticleGoogle Scholar
- Naismith TV, Heuser JE, Breakefield XO, Hanson PI: TorsinA in the nuclear envelope. Proc Natl Acad Sci U S A 2004, 101: 7612-7617. 10.1073/pnas.0308760101View ArticleGoogle Scholar
- Naismith TV, Dalal S, Hanson PI: Interaction of torsinA with its major binding partners is impaired by the dystonia-associated DeltaGAG deletion. J Biol Chem 2009, 284: 27866-27874. 10.1074/jbc.M109.020164View ArticleGoogle Scholar
- Nery FC, Zeng J, Niland BP, Hewett J, Farley J, Irimia D, Li Y, Wiche G, Sonnenberg A, Breakefield XO: TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton. J Cell Sci 2008, 121: 3476-3486. 10.1242/jcs.029454View ArticleGoogle Scholar
- Neuwald AF, Aravind L, Spouge JL, Koonin EV: AAA+: A class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes. Genome Res 1999, 9: 27-43.Google Scholar
- Ozelius LJ, Hewett JW, Page CE, Bressman SB, Kramer PL, Shalish C, de Leon D, Brin MF, Raymond D, Corey DP, Fahn S, Risch NJ, Buckler AJ, Gusella JF, Breakefield XO: The early-onset torsion dystonia gene (DYT1) encodes an ATP-binding protein. Nat Genet 1997, 17: 40-48. 10.1038/ng0997-40View ArticleGoogle Scholar
- Sosa BA, Demircioglu FE, Chen JZ, Ingram J, Ploegh H, Schwartz TU: How lamina-associated polypeptide 1 (LAP1) activates torsin. eLife 2014, 3: e03239.View ArticleGoogle Scholar
- Tanabe LM, Kim CE, Alagem N, Dauer WT: Primary dystonia: molecules and mechanisms. Nat Rev Neurol 2009, 5: 598-609. 10.1038/nrneurol.2009.160View ArticleGoogle Scholar
- Vander Heyden AB, Naismith TV, Snapp EL, Hodzic D, Hanson PI: LULL1 retargets TorsinA to the nuclear envelope revealing an activity that is impaired by the DYT1 dystonia mutation. Mol Biol Cell 2009, 20: 2661-2672. 10.1091/mbc.E09-01-0094View ArticleGoogle Scholar
- Vander Heyden AB, Naismith TV, Snapp EL, Hanson PI: Static retention of the lumenal monotopic membrane protein torsinA in the endoplasmic reticulum. EMBO J 2011, 30: 3217-3231. 10.1038/emboj.2011.233View ArticleGoogle Scholar
- Wittig I, Braun HP, Schagger H: Blue native PAGE. Nat Protoc 2006, 1: 418-428. 10.1038/nprot.2006.62View ArticleGoogle Scholar
- Zacchi LF, Wu HC, Bell SL, Millen L, Paton AW, Paton JC, Thomas PJ, Zolkiewski M, Brodsky JL: The BiP molecular chaperone plays multiple roles during the biogenesis of torsinA, an AAA+ ATPase associated with the neurological disease early-onset torsion dystonia. J Biol Chem 2014, 289: 12727-12747. 10.1074/jbc.M113.529123View ArticleGoogle Scholar
- Zhao C, Brown RS, Chase AR, Eisele MR, Schlieker C: Regulation of Torsin ATPases by LAP1 and LULL1. Proc Natl Acad Sci U S A 2013, 110: E1545-E1554. 10.1073/pnas.1300676110View ArticleGoogle Scholar
- Zhu L, Wrabl JO, Hayashi AP, Rose LS, Thomas PJ: The torsin-family AAA+ protein OOC-5 contains a critical disulfide adjacent to Sensor-II that couples redox state to nucleotide binding. Mol Biol Cell 2008, 19: 3599-3612. 10.1091/mbc.E08-01-0015View ArticleGoogle Scholar
- Zolkiewski M, Wu HC: Emerging area: TorsinA, a novel ATP-dependent factor linked to dystonia, in protein chaperones and protection from neurodegenerative diseases. Edited by: Witt SN. Hoboken, New Jersey: John Wiley & Sons; 2011.Google Scholar
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