- Open Access
Self-parameterized active contours based on regional edge structure for medical image segmentation
© Mylona et al.; licensee Springer. 2014
- Received: 25 June 2014
- Accepted: 24 July 2014
- Published: 11 August 2014
This work introduces a novel framework for unsupervised parameterization of region-based active contour regularization and data fidelity terms, which is applied for medical image segmentation. The work aims to relieve MDs from the laborious, time-consuming task of empirical parameterization and bolster the objectivity of the segmentation results. The proposed framework is inspired by an observed isomorphism between the eigenvalues of structure tensors and active contour parameters. Both may act as descriptors of the orientation coherence in regions containing edges. The experimental results demonstrate that the proposed framework maintains a high segmentation quality without the need of trial-and-error parameter adjustment.
- Active contours
- Unsupervised parameterization
- Structure tensors
- Medical image segmentation
Most medical image segmentation methods are parameterized empirically by medical doctors (MDs), who are unfamiliar with the intrinsic mechanisms of the underlying algorithms. Consequently, they depend on technical support by engineers. A key challenge is to develop reliable unsupervised parameterization approaches in order to set MDs free from the cumbersome and time-consuming process of parameter tuning, as well as to bolster the objectivity and reliability of the segmentation results.
Region-based active contours are prominent in medical image segmentation, due to their inherent noise-filtering mechanism (Ferrari et al. 2004; Liao et al. 2011; Petroudi et al. 2010; Xu et al. 2010). Data fidelity and regularization parameters are of critical importance in region-based active contour segmentation, since they act as amplifiers on the forces guiding contour evolution (Chan & Vese 2001; Li et al. 2010; Liu et al. 2011; Sundaramoorthi et al. 2008). Parameter values are typically adjusted in an empirical fashion and kept fixed for the entire spatial extent of an image. Thus, forces are uniformly weighted until convergence, regardless of the local image content.
Researchers have tried to confront the issue of empirical parameterization of region-based active contours by balancing the trade-off between regularization and data fidelity forces. However, each individual parameter is still empirically adjusted, as in the case of (Ma & Yu 2010). McIntosh and Hamarneh (McIntosh & Hamarneh 2007) adapt regularization weights across a set of images. Even though one weight may be optimal for some regions in an image, the same weight may not be optimal for all regions. Moreover, the data fidelity parameter is still empirically determined. Erdem and Tari (Erdem & Tari 2009) utilize data-driven local cues focusing on edge consistency and texture cues. Nevertheless, this method requires technical skills from the end-user. Dong et al. (2008) present an algorithm to capture brain aneurysms from the vascular tree, by varying the regularization term based on the surface curvature of a pre-segmented vessel. However, the regularization weight does not rely on image content. On the contrary, it depends on the shape of the target region, thus limiting the applicability of the method on different target shapes.
This work introduces a novel framework for unsupervised parameterization of region-based active contours, which is applicable on medical image segmentation. The proposed framework is inspired by the observation of an isomorphism between the eigenvalues of structure tensors and the active contour regularization and data fidelity parameters. The former are capable of describing the orientation coherence of regions containing edges. In a similar fashion, active contour parameters can be derived from a function encoding orientation coherence. The function used in the context of the proposed framework is the orientation entropy (OE). This measure obtains low values in structured regions, which contain edges with low orientation variability, and high values in unstructured regions, which contain edges of multiple orientations. Accordingly, OE is capable to adjust forces driving the contour away from unstructured edge regions and guide it towards more structured ones, which are naturally associated with the boundaries of medical objects.
Regularization and data fidelity terms are self-parameterized in a spatially-varying fashion, as dictated by OE, facilitating the identification of the actual target boundaries, without the requirement for empirical fine-tuning or user-intervention. A byproduct of the proposed framework is that iterations dedicated to false local minima are bypassed, speeding up contour convergence. Any erroneous behavior in the early or middle stages of contour evolution is not propagated, since parameters hinge on information obtained solely from the image content and not from the possibly misleading contour shape.
It should be stressed that the proposed framework: 1) is not focused on convergence acceleration, which is only a byproduct, 2) is not proposed to substitute or outperform any of the numerous state-of-the-art active contour variations. Instead of these, the proposed framework primarily aims at unsupervised parameterization of region-based active contours, so as to achieve a segmentation quality which is comparable to the one obtained by the empirically fine-tuned version. In addition, the process for the identification of edge regions should not be confused with traditional edge detection, which focuses on single edges, often generated by noise.
The proposed framework is applicable to various medical imaging modalities and due to its simplicity and flexibility, can be embedded in various region-based active contour variations. Moreover, it is not sensitive on alterations in the settings of the acquisition devices. Such alterations, often required in clinical practice, naturally affect the acquired image features. As a result, in the case of empirical parameterization, manual fine-tuning might be required on a per-image basis, raising doubts on the actual value of a computational segmentation approach. A preliminary variation of this work appeared in (Mylona et al. 2012). Although the variation proposed in this study obtains high segmentation quality, it is not based on the valuable directional information derived by multi-directional filtering, whereas it has only been applied on a limited number of images.
The remainder of this paper is organized as follows: Section 2 describes the proposed framework and Section 3 presents the experimental results. The conclusions of this study are summarized in Section 4.
where the + sign belongs to λ1. It is worth noting that λ1 is the principal eigenvalue and is longitudinal with respect to the principal axis of the tensor ellipsoid, whereas λ2 is the minor eigenvalue and is vertical with respect to the same principal axis. Additionally, λ1 and λ2 integrate the variation of intensity values within a region and reflect the orientation coherence along the corresponding eigenvectors.
where E reg and E df are associated with regularization and data fidelity energy terms, respectively, whereas w reg and w df are weighting parameters. Regularization forces are most likely tangent to the principal axis of the contour whereas data fidelity forces are vertical to this axis, attracting the contour towards target edges. It is tempting to notice that if we associate the principal axis of the tensor ellipsoid with the principal axis of the contour, the regularization weight w reg corresponds to the same direction as the principal eigenvalue λ1, whereas the data fidelity weight w df corresponds to the same direction as the minor eigenvalue λ2. This premise indicates a link between the active contour parameters and the eigenvalues of the structure tensor.
where I jk is a subband image generated by a multi-directional filtering method (Do & Vetterli 2005), OE jk is the OE of the subband image I jk in the k th direction and the j th level of decomposition, p jk is the probability function, M jk is the row size and N jk the column size of the subband image. It should be highlighted that OE is calculated on each directional frequency of the original image, generated by the contourlet transform (CT) (Do & Vetterli 2005) and not on the original image, since the former is capable to highlight the orientation coherence in edge regions.
The main idea is to navigate the contour towards structured, target regions (blue rectangle of Figure 1(c)) in the early stages of evolution. This is achieved by assigning high values of OE to w df which will appropriately amplify data fidelity forces in randomly oriented, high-entropy regions. Hence, iterations dedicated to erroneous local minima will be bypassed, speeding up contour convergence towards target edges. It should be noted that both parameters are calculated only once at initialization. The aim is to guide the contour directly to target edge regions, already from the beginning, and to prevent any erroneous behavior during evolution by ‘constantly reminding’ where the target edge regions lie. Furthermore, by setting regularization terms as the reciprocal of data fidelity terms, the proposed framework achieves a balanced trade-off between regularization and data fidelity parameters. This is of primary importance in cases of unstructured edge regions where data provide a less reliable clue than contour regularization.
where ϕ is the level set function, I the observed image, c1, c2 the average intensities inside and outside of the contour, respectively, the fixed regularization parameter and the fixed data fidelity parameter. For the empirical case, parameters and obtain the optimal values 0.006 ⋅ 2552 and 1, respectively, according to the original paper (Chan & Vese 2001). For the proposed framework, the regularization and data fidelity parameters are automatically calculated according to (6). In addition, contour is initialized in the proximity of the target region and remains the same for both the proposed framework and the empirically fine-tuned version so as to facilitate fair comparisons.
Experiments are conducted on databases of various medical imaging modalities so as to confirm the framework’s generality with respect to image content. All image modalities were investigated by MDs who provided ground truth images. The shape of all abnormalities as well as the irregularity of their margins are malignant risk factors which are highly considered by MDs before proceeding to fine needle aspiration biopsy.
The first database consists of 50 mammographic images containing abnormalities randomly obtained by the mini-MIAS database (Suckling et al. 1994). The background tissue is characterized as: a) fatty, b) fatty-glandular and c) dense-glandular, whereas the abnormality is classified as: a) well-defined/circumscribed and b) ill-defined. In terms of its severity, the abnormality is defined as benign or malignant.
The second database consists of 45 thyroid ultrasound images containing hypoechoic nodules provided by the Radiology Department of Euromedica S.A., Greece. All ultrasound images were acquired using a digital ultrasound imaging system HDI 3000 ATL with a 5-12 MHz linear transducer. Instrument settings were fixed accordingly to the built-in ‘SmallPartTest’ Philips protocol, magnification was set to 1:1 and dynamic range was set to 150 dB/C4. Hypoechoic nodules with regular boundaries may represent follicular neoplasms of medium-risk, whereas hypoechoic nodules with irregular boundaries are considered suspicious for malignancy and may represent thyroid carcinomas.
The third database consists of 32 endoscopy frame images containing polyps provided by the Gastroenterology Section, Department of Pathophysiology, Medical School, University of Athens, Greece and partially by the Section for Minimal Invasive Surgery, University of Tübingen, Germany. The endoscopic data was acquired from sixty-six different patients with an Olympus CF-100 HL endoscope. All frame images consist of small size adenomatous polyps which are not easily detectable and are more likely to become malignant.
The fourth database consists of 40 labial teeth and gingiva photographic images randomly obtained by the LTG-IDB database (Eckhard et al. 2012) created by the Color Imaging Lab at the Optics Department of the University of Granada, Spain. For the image acquisition, a Canon EOS 7D digital single-lens reflex color camera combined with a Canon EFS 18-135 mm standard zoom lens was used. The scope of this database usage is the task of teeth/non-teeth segmentation.
It should be noted that the size of the images obtained by the first and second database is 256 × 256 and the size of the images obtained by the third, fourth and fifth databases is 320 × 320. For an image of size 256 × 256, an image grid of size 32 × 32 is considered suitable and is fed into CT through an iterative procedure. The image grid is further decomposed to four subbands of size 16 × 16. For an image of size 320 × 320, an image grid of size 40 × 40 is considered suitable for CT decomposition. In this case, the obtained four subbands’ size is 20 × 20. The size of the image grid is experimentally determined as the minimum of the negative power of two of the original image size, which maintains an edge region.
The segmentation results presented in Figure 3 demonstrate that the proposed framework achieves comparable segmentation quality to the one obtained by the empirically fine-tuned version in an unsupervised fashion.
- a)the Tannimoto coefficient (TC) (Crum et al. 2006), which is defined by:(8)
where A is the region identified by the segmentation method under evaluation, B is the ground truth region and N() indicates the number of pixels of the enclosed region and
- b)the Hausdorff distance H (Huttenlocher et al. 1993) defined as:(9)
where is called the directed Hausdorff distance from A to B, A is the ground truth set, B the set under evaluation and a, b the points defined in sets A, B, respectively.
The unsupervised version achieves an average TC and H value of 82.9 ± 1.6% and 42.3 ± 1.3 mm, respectively with regards to all images tested, which is comparable to the TC and H value of 80.7 ± 1.8% and 40.9 ± 1.5 mm, respectively obtained by the empirically fine-tuned version. This comparable segmentation accuracy verifies the value of the proposed framework for unsupervised parameter adjustment.
In this work, a novel framework for unsupervised adjustment of active contour regularization and data fidelity parameters is presented and applied for medical image segmentation. The work is motivated by the need for unsupervised parameterization, which is prominent in medical imaging applications, so as to relieve MDs from the laborious, time-consuming task of empirical parameterization and bolster the objectivity of the segmentation results.
The proposed framework is inspired by an isomorphism between these parameters and the eigenvalues of structure tensors. Considering that the latter provide essential information associated with the orientation coherence of edge regions, we encode this information in parameters by means of OE, which obtains high values in unstructured edge regions and low values in structured ones. In this way, region-based forces drive the contour away from non-target, unstructured regions and navigate it towards the target, structured ones, which are naturally associated with the boundaries of medical objects (Figure 1).
The proposed framework is validated on several medical image databases by comparing its segmentation performance with the empirically fine-tuned version. The experimental results demonstrate that the unsupervised version maintains a high segmentation quality, comparable to the one obtained empirically, yet in an unsupervised fashion. Hence, MDs are set free from the laborious process of empirical parameterization and the objectivity of the results is enhanced. Moreover, contour convergence is accelerated.
Although this work addresses the medical imaging domain, demonstrating the effectiveness of the proposed framework in various medical imaging modalities, in principle it can also be applied to other imaging domains. This could be the subject of a future study, since it is out of the scope of this work. Another future direction involves the integration of the proposed framework on different region-based active contour variations.
This work has been co-financed by the European Union (European Social Fund-ESF) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) - Research Funding Program: Heracleitus II. Investing in knowledge society through the European Social Fund. We would like to thank the Gastroenterology Section, Department of Pathophysiology, Medical School, University of Athens, Greece for the provision of endoscopic data as well as Prof. M. Tzivras and his research group for the provision of the ground truth. We would also like to thank Dr. N. Dimitropoulos, MD Radiologist, and EUROMEDICA S.A., Greece, for the provision of the thyroid ultrasound images as well as their ground truth.
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