In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation
© Mohd Hilmi et al.; licensee Springer. 2013
Received: 23 December 2012
Accepted: 20 January 2013
Published: 5 March 2013
Chitosan is a marine-derived product that has been widely used in clinical applications, especially in skin reconstruction. The mammalian scaffolds derived from bovine and porcine material have many limitations, for example, prion transmission and religious concerns. Therefore, we created a chitosan skin regenerating template (SRT) and investigated the behavior of fibroblast cell-scaffold constructs. Primary human dermal fibroblasts (HDF) were isolated and then characterized using vimentin and versican. HDF were seeded into chitosan SRT at a density of 3×106 cells/cm2 for fourteen days. Histological analysis and live cells imaging revealed that the cell-chitosan constructs within interconnected porous chitosan showed significant interaction between the cells as well as between the cells and the chitosan. Scanning electron microscopy (SEM) analysis revealed cells spreading and covering the pores. As the pore sizes of the chitosan SRT range between 40–140 μm, an average porosity is about 93 ± 12.57% and water uptake ratio of chitosan SRT is 536.02 ± 14.29%, it is a supportive template for fibroblast attachment and has potential in applications as a dermal substitute.
Chitosan is a biopolymer (β-1,4-D-glucosamine) derivative of chitin (poly-N-acetylglucosamine) that has become an excellent biomedical resource in clinical medicine, especially in dermatology and plastic reconstructive surgery. Chitosan biomaterials are normally used to cover full-thickness skin defects resulting from traumas (Halim et al. 1998; Pusateri et al.2003), burns (Jin et al. 2007 ; Guo et al. 2011) and skin ulcers (Anandan et al. 2004 ; Park et al. 2009). The recent use of chitosan in regenerative medicine, particularly in skin tissue engineering, has been beneficial for the treatment of full-thickness skin defects (Tchemtchoua et al. 2011).
Chitosan was reported to improve homeostasis (Kozen et al. 2008) and promote granulation by enhancing the function of inflammatory cells such as leukocytes, fibroblasts and macrophages (Ueno et al. 2001). Furthermore, chitosan has antimicrobial effects against bacteria, fungi and viruses (Rabea et al. 2003). In addition to the skin, chitosan scaffolds also stimulate the regeneration of spinal cord (Li et al. 2009), cornea (Auxenfans et al. 2009) and liver (Wang et al. 2008) tissues in their respective three-dimensional (3-D) environments.
As a marine product, chitosan has many advantages compared with other collagen biomaterials that are prepared from bovine or porcine sources. These mammalian products have resulted in the transmission of prions to humans. In contrast, marine-derived products are safe for human use due to the species barrier (Prusiner 1998).
Fibroblasts are mesenchymal cells that provide tissue maintenance and support by secreting extracellular matrix (ECM) in connective tissue. In the full-thickness skin model, cultured allogenic fibroblasts in 3-D scaffolds induce inflammation and scar formation, which are important events in wound healing (Lamme et al. 2002). This model has been reported to improve the regeneration of dermis (Lamme et al. 2000) and epidermis (El-Ghalbzouri et al. 2002). Van den Bogaerd et al. (2002) demonstrated that dermis is the best source for skin tissue engineering applications, based on fibroblast yields and the reduced contraction of the wound.
Recently, a study conducted by Lim et al. (2010) showed that chitosan porous skin regenerating templates (SRT) are biocompatible, as determined using primary human keratinocytes with the direct-contact method. They found that chitosan porous SRT did not induce additional inflammatory responses or DNA damage. Their data highlighted the potential use of chitosan porous SRT in skin tissue engineering. However, its potential as a scaffold to support cell attachment and proliferation is still unexplored.
Therefore, this study investigated the ability of a 3-D structure of chitosan porous SRT to support human fibroblast spreading and viability. Chitosan SRT supports fibroblast attachment and has potential for use in skin tissue engineering applications especially in the de novo fabrication of dermal substitutes.
Results and discussion
Isolation and characterization of HDF
Characterization of chitosan SRT, cell attachment and proliferation in vitro
Tabulated data for the characterization of chitosan SRT (mean ± SEM, n = 6)
5.02 ± 0.73 N/m2
Average pore diameter
39.41 ± 15.11 μm
143.55 ± 18.21 μm
93.21 ± 12.57%
3.97 ± 0.34 mg/cm2 hr
Water uptake ratio
536.02 ± 14.29%
In vitro degradation
35 ± 5 days
OD 570 value of fibroblasts into chitosan (mean ± SEM, n = 4)
As a graded porous scaffold, chitosan SRT mimics the natural porous structure more closely than a uniformly porous scaffold. Uniformly porous scaffolds have many limitations. They only allow one cell type to grow depending on the pore size, whereas graded porous scaffold regenerated multiple types of tissue simultaneously (Oh et al. 2007). Yannas et al. (1989) found that the optimum pore size for skin regeneration templates ranged between 20 – 125 μm. Hence, chitosan SRT fulfills this criterion and is suitable as a template for skin tissue engineering.
The ability of chitosan to adsorb nutrients involved in wound healing and exudation of the wound bed is important factors in skin regeneration. Therefore, the determination of water adsorption of chitosan is important to enhance the biological activity of a dermal equivalent. The water uptake ratio of chitosan SRT (536.02 ± 14.29%) was higher than reported previously by Shi et al. (2006). It can attribute to hydrophilicity and the maintenance of 3-D structure. Scaffold with high water uptake ratio is suitable both in full thickness wound that contains excess exudates and chronic inflammation that leading to impair wound healing. To enhance the dermal regeneration and wound closure, it is important to maintain a moist of wound bed. They can be achieved if scaffold has suitable water vapor permeability (WVP). The WVP of chitosan SRT (3.97 ± 0.34 mg/cm2 hr) was higher than suggested by Lamke et al. (1977). It happened because of chitosan SRT adsorbed more water. Therefore, more water was vaporized. The WVP depended on scaffold thickness and the ratio of scaffold area to water surface area (Hu et al. 2001). The WVP must be suitable for wound dressing. If the WVP is too high, the wound bed will dry and will increase the metabolism activity. Contrary, if the WVP is too low, the accumulation of exudates will trigger the onset of bacterial growth. The tensile strength of chitosan SRT was determined as 5.02 ± 0.73 MPa. Jansen and Rottier (1958) reported that the tensile strength for male skin ranged between 3 – 14 MPa. Meanwhile, for female skin, the ranged was 4 – 13 MPa. Therefore, chitosan SRT is suitable for both male and female skin regeneration.
In conclusion, we demonstrated that a chitosan SRT supports HDF attachment. The presence of interconnected pores of both macroscopic and microscopic size in the chitosan is considered as a factor in regulating the survival and attachment of the cell-chitosan construct. Furthermore, the physical and mechanical properties of chitosan SRT are useful for the cultivation of HDF for dermal regeneration. To the best of our knowledge, this is the first microscopic study to analyze the integration of HDF and chitosan SRT.
This research was approved by the Research Ethics Committee (Human) of Universiti Sains Malaysia (approval code: USMKK/PPP/JEPeM [212.3(15)]).
Isolation and characterization of HDF
Skin samples were obtained from ten consenting patients who underwent plastic surgery (according to a protocol approved by an ethics committee and in accordance with the Declaration of Helsinki). To avoid contamination and to maintain cellular integrity, the skin was dissected within 24 hours. It was cut into small pieces, each approximately 0.3 cm2 to 0.5 cm2, in a Petri dish (Nunc, Denmark) containing phosphate-buffered saline (PBS) (Gibco, USA). PBS was used for washing off cell debris, including fat and coagulated blood. To separate the epidermis and dermis, the skin pieces were incubated in William’s E Medium (Sigma, Germany) supplemented with 0.1% (w/v) reconstituted dispase (Invitrogen, Japan) and 1% (v/v) antimycotic (Invitrogen, USA) overnight at 4°C or for two to three hours at 37°C. After the epidermis was discarded, the dermis was incubated in William’s E Medium (Sigma, Germany) containing 0.1% (w/v) collagenase type I (Gibco, USA) overnight in a 37°C incubator-roller (Binder, Germany). After vigorous resuspension, the cell suspensions were filtered using 70-μm strainers (BD Falcon, USA) to separate the hair shafts and agglutinated cells. The William’s E Medium was dispensed onto the strainer to increase the yield of cells. Human dermal fibroblasts (HDF) were collected by centrifugation (Hettichzentrifugen, Germany) at 420 × g for 5 minutes and cultured in 6-well plates (Nunc, Denmark) at a density of 1×105 cells per well in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen, USA) supplemented with 1% (v/v) antimycotic and 10% (v/v) fetal bovine serum (FBS) (Invitrogen, USA). The HDF cultures were monitored daily to confirm that there was no contamination.
For characterization experiments, HDF cells from passages two to five were used. The cells were seeded on a 4-well chamber slide (Nunc, Denmark) at a density of 1×103 cell/ml and fixed with cold methanol (Merck, Germany) for 10 minutes at −20°C. Indirect immunofluorescence staining was performed using mouse monoclonal antibodies against human vimentin (Abcam, UK) and human versican (Abcam, UK). The negative control was performed using mouse IgG1 negative control (1:500) (Serotec, USA). Incubations with the primary antibody (1:500) and fluorescent secondary antibody against mouse IgG (1:500) (Abcam, UK) were performed at room temperature for one hour or 45 minutes respectively. To stain the nuclei, 4', 6-diamidino-2-phenylindole (DAPI) (1:500) (Sigma, Germany) was used. Images were captured using a laser scanning microscope (LSM) (Zeiss, Germany).
Fabrication and characterization of chitosan SRT
Ultrapure medical-grade chitosan powder (Hunza Nutriceuticals, Malaysia) was produced from prawn shell. It was irradiated with 10 kGy of gamma radiation to produce a molecular weight of 440,000 Daltons and dissolved in 0.5 M acetic acid. Distilled water was added and the mixture was heated at 70°C for seven hours in the oven. The mixture was cooled to room temperature, 20% (w/v) glycerol was added and pH was adjusted to 6.2 with 5% (w/v) sodium bicarbonate (NaHCO3). The mixture formed a gel to which distilled water was added until the final concentration of the chitosan solution was 2% (w/v). To obtain an inner porous sponge layer, the chitosan solution was poured into a 10 cm by 10 cm polytetrafluoroethylene (PTFE) mold, deep-frozen at −20°C for 24 hours and freeze-dried for 20 hours. The porosity and pore size were controlled by varying the freezing rate which resulted in the formation of ice crystals of varying sizes. Nucleation of the ice crystals was performed by the application of thermal gradients. Removal of the ice crystal by lyophilization generated a porous material. The pore size decreased as the freezing temperature increased. The pores were orientated by controlling the geometry of the thermal gradients during the freezing process.
To determine the pore size, chitosan was punched into small discs that were 15 mm in diameter. The pore diameter was determined at the disc surface by scanning electron microscope (SEM) (Leo, UK). The porosity was calculated using the formula V m /V p × 100%, where V m is the volume of the pores and V p is the volume of chitosan.
To determine biodegradation, chitosan was incubated in PBS containing 1000 unit/ml of lysozyme for 40 days at 37°C. The chitosan was washed with distilled water and freeze-dried. In vitro degradation, D, was calculated using the formula D= (W 0 – W 1 )/W 0 × 100%, where W 0 is the original weight and W 1 is the weight at the time of measurement.
where F is force, A is the cross-sectional area of the chitosan, ΔL is its total elongation (change in length) and L is its original length.
The water uptake ratio was determined using the formula W 1 -W o /W o × 100%, where W o is the initial weight and W 1 is the wet weight after incubating the chitosan in PBS at room temperature for 24 hours.
The water vapor permeability (WVP) of the chitosan was determined using the flexible bottles permeation test (Systech, UK). The bottles were stored at room temperature for 5 hours and the mass of water lost from the bottles was monitored as a function of time. WVP was calculated at steady-state using the formula WVP = W/AT where W is the mass of water lost, A is the area (1.18 cm2) and T is the exposure time.
3-D cultivation of HDF
For 3-D cultivation, chitosan disc of 5 mm diameter and 2 mm thickness were used. The chitosan discs were put into a 96-well plate and initially seeded with 40 μl of culture medium containing cells at a density of 3×106/cm2. The first 20 μl of cells were dispensed using a micropipette onto the center of each chitosan disc. After these cells were adsorbed into the chitosan, the remaining cells were dispensed at its edge. After 2 hours, another 100 μL of culture medium was added to ensure adequate cells proliferation. After 6 hours, the chitosan discs were transferred into 24-well plates to ensure that the cells had enough growth medium for 3-D proliferation. The cultured chitosan discs were incubated in 5% CO2 at 37°C. The growth medium was changed every day. On day 14, the chitosan discs with the 3-D cell cultures were harvested for further experiments.
Attachment of HDF onto chitosan SRT and viability analysis
The viability of cells grown into chitosan was analyzed using a cell viability kit (Invitrogen, USA). The 3-D cell cultures grown into chitosan were incubated with calcein and ethidium, (1:4) in 2 mL PBS to stain live or dead cells respectively. The incubation was performed in 24-well culture plates (Orange Scientific, Belgium) at room temperature for 30 minutes. Chitosan without cells was used as a control. The 3-D cultivated live cells were imaged using a laser scanning confocal microscope (LSCM) (Zeiss, Germany).
To observe the interactions of HDF onto chitosan, the 3-D cells cultures were washed twice with PBS and fixed with 2.5% glutaraldehyde for an hour at 4°C. Serial dehydration using a graded ethanol series of 30, 60 and 100% was performed for 5 minutes each followed by air drying at 37°C for 4 hours. Prior to imaging, the surface of chitosan was sputter-coated with gold (Leica, Czech Republic). Images were captured using SEM (Quanta, Netherlands).
The attachment of cells into 3-D chitosan scaffold was further analyzed by hematoxylin and eosin staining. Briefly, 3-D cultured cells into the chitosan scaffold were fixed with 10% formalin, embedded in paraffin and sectioned at 5 μm thickness. The attachment of cells into the chitosan was viewed using a Mirax Desk scanner (Zeiss, Germany).
To evaluate the viability of cells in 3-D culture, HDF from four independent samples were cultured in a 24-well plate at a density of 3×106/cm2. Each well including one with chitosan but without cells as a negative control, was incubated with Presto Blue Cell Viability Reagent (Invitrogen, USA) (1:10) for 24 hours. The medium (100 μl) from each well was transferred into a 96-well plate (Orange, Belgium) and analyzed using a Nano Quant enzyme-linked immunosorbent assay reader (Tecan, Austria). The absorbance was determined at 570 nm with a reference wavelength of 600 nm.
The data are presented as the mean ± SEM.
This study was supported by the Universiti Sains Malaysia Research University Grant (1001/PPSP/812037) and the Research University Postgraduate Research Grant Scheme (1001/PPSP/8144012). We thank the Craniofacial Laboratory staff for their permission and guidance in using the latest machines in PPSG. We are most grateful to Rageshwari Ramupillai from Carl Zeiss (M) for her special technical assistance, support and commitment. The generous support of Mohd Hafiz from SIRIM is gratefully acknowledged.
- Anandan R, Nair PGV, Mathew S: Anti-ulcerogenic effect of chitin and chitosan on mucosal antioxidant defence system in hcl-ethanol-induced ulcer in rats. J Pharm Pharmacol 2004, 56: 265-269. 10.1211/0022357023079View ArticleGoogle Scholar
- Auxenfans C, Builles N, Andre V, Lequeux C, Fievet A, Rose S, Braye FM, Fradette J, Janin-Manificat H, Nataf S, Burillon C, Damour O: Porous matrix and primary-cell culture: A shared concept for skin and cornea tissue engineering. Pathol Biol (Paris) 2009, 57: 290-298. 10.1016/j.patbio.2008.04.014View ArticleGoogle Scholar
- Chang HY, Chi J-T, Dudoit S, Bondre C, van de Rijn M, Botstein D, Brown PO: Diversity, topographic differentiation, and positional memory in human fibroblasts. Proceedings of the National Academy of Sciences 2002, 99: 12877-12882. 10.1073/pnas.162488599View ArticleGoogle Scholar
- Chen VJ, Ma PX: Nano-fibrous poly(l-lactic acid) scaffolds with interconnected spherical macropores. Biomaterials 2004, 25: 2065-2073. 10.1016/j.biomaterials.2003.08.058View ArticleGoogle Scholar
- Chen FG, Zhang WJ, Bi D, Liu W, Wei X, Chen FF, Zhu L, Cui L, Cao Y: Clonal analysis of nestin- vimentin+ multipotent fibroblasts isolated from human dermis. J Cell Sci 2007, 120: 2875-2883. 10.1242/jcs.03478View ArticleGoogle Scholar
- El-Ghalbzouri A, Gibbs S, Lamme E, Van Blitterswijk CA, Ponec M: Effect of fibroblasts on epidermal regeneration. Br J Dermatol 2002, 147: 230-243. 10.1046/j.1365-2133.2002.04871.xView ArticleGoogle Scholar
- Even-Ram S, Yamada KM: Cell migration in 3d matrix. Curr Opin Cell Biol 2005, 17: 524-532. 10.1016/j.ceb.2005.08.015View ArticleGoogle Scholar
- Faigle W, Colucci-Guyon E, Louvard D, Amigorena S, Galli T: Vimentin filaments in fibroblasts are a reservoir for snap23, a component of the membrane fusion machinery. Mol Biol Cell 2000, 11: 3485-3494.View ArticleGoogle Scholar
- Frederick G: Fibroblast biology in three-dimensional collagen matrices. Trends Cell Biol 2003, 13: 264-269. 10.1016/S0962-8924(03)00057-6View ArticleGoogle Scholar
- Guo R, Xu S, Ma L, Huang A, Gao C: The healing of full-thickness burns treated by using plasmid DNA encoding vegf-165 activated collagen-chitosan dermal equivalents. Biomaterials 2011, 32: 1019-1031. 10.1016/j.biomaterials.2010.08.087View ArticleGoogle Scholar
- Halim AS, Stone CA, Devaraj VS: The hyphecan cap: A biological fingertip dressing. Injury 1998, 29: 261-263.View ArticleGoogle Scholar
- Hattori N, Carrino DA, Lauer ME, Vasanji A, Wylie JD, Nelson CM, Apte SS: Pericellular versican regulates the fibroblast-myofibroblast transition. J Biol Chem 2011, 286: 34298-34310. 10.1074/jbc.M111.254938View ArticleGoogle Scholar
- Hu Y, Topolkaraev V, Hiltner A, Baer E: Measurement of water vapor transmission rate in highly permeable films. Journal of Applied Polymer Science 2001, 81: 1624-1633. 10.1002/app.1593View ArticleGoogle Scholar
- Jansen L, Rottier P: Comparison of the mechanical properties of strips of human abdominal skin excised from below and from above the umbilic. Dermatology 1958, 117: 252-258. 10.1159/000255597View ArticleGoogle Scholar
- Jin Y, Ling P-X, He Y-L, Zhang T-M: Effects of chitosan and heparin on early extension of burns. Burns 2007, 33: 1027-1031. 10.1016/j.burns.2006.12.002View ArticleGoogle Scholar
- Johnston JA, Ward CL, Kopito RR: Aggresomes: A cellular response to misfolded proteins. The Journal of Cell Biology 1998, 143: 1883-1898. 10.1083/jcb.143.7.1883View ArticleGoogle Scholar
- Katsumoto T, Mitsushima A, Kurimura T: The role of the vimentin intermediate filaments in rat 3y1 cells elucidated by immunoelectron microscopy and computer-graphic reconstruction. Biol Cell 1990, 68: 139-146. 10.1016/0248-4900(90)90299-IView ArticleGoogle Scholar
- Kozen BG, Kircher SJ, Henao J, Godinez FS, Johnson AS: An alternative hemostatic dressing: Comparison of celox, hemcon, and quikclot. Acad Emerg Med 2008, 15: 74-81. 10.1111/j.1553-2712.2007.00009.xView ArticleGoogle Scholar
- Lamke LO, Nilsson G, Reithner H: The evaporative water loss from burns and the water-vapour permeability of grafts and artificial membranes used in the treatment of burns. Burns 1977, 3: 159-165. 10.1016/0305-4179(77)90004-3View ArticleGoogle Scholar
- Lamme EN, Van Leeuwen RTJ, Brandsma K, Van Marle J, Middelkoop E: Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation. The Journal of Pathology 2000, 190: 595-603. 10.1002/(SICI)1096-9896(200004)190:5<595::AID-PATH572>3.0.CO;2-VView ArticleGoogle Scholar
- Lamme EN, van Leeuwen RT, Mekkes JR, Middelkoop E: Allogeneic fibroblasts in dermal substitutes induce inflammation and scar formation. Wound Repair Regen 2002, 10: 60-152.View ArticleGoogle Scholar
- Li X, Yang Z, Zhang A, Wang T, Chen W: Repair of thoracic spinal cord injury by chitosan tube implantation in adult rats. Biomaterials 2009, 30: 1121-1132. 10.1016/j.biomaterials.2008.10.063View ArticleGoogle Scholar
- Lim CK, Yaacob NS, Ismail Z, Halim AS: In vitro biocompatibility of chitosan porous skin regenerating templates (psrts) using primary human skin keratinocytes. Toxicology in Vitro 2010, 24: 721-727. 10.1016/j.tiv.2010.01.006View ArticleGoogle Scholar
- Ma PX, Choi J-W: Biodegradable polymer scaffolds with well-defined interconnected spherical pore network. Tissue Eng 2001, 7: 23-33. 10.1089/107632701300003269View ArticleGoogle Scholar
- Oh SH, Park IK, Kim JM, Lee JH: In vitro and in vivo characteristics of pcl scaffolds with pore size gradient fabricated by a centrifugation method. Biomaterials 2007, 28: 1664-1671. 10.1016/j.biomaterials.2006.11.024View ArticleGoogle Scholar
- Park CJ, Clark SG, Lichtensteiger CA, Jamison RD, Johnson AJW: Accelerated wound closure of pressure ulcers in aged mice by chitosan scaffolds with and without bfgf. Acta Biomaterialia 2009, 5: 1926-1936. 10.1016/j.actbio.2009.03.002View ArticleGoogle Scholar
- Peter XM: Biomimetic materials for tissue engineering. Advanced Drug Delivery Reviews 2008, 60: 184-198. 10.1016/j.addr.2007.08.041View ArticleGoogle Scholar
- Prusiner SB: Prions. Proceedings of the National Academy of Sciences 1998, 95: 13363-13383. 10.1073/pnas.95.23.13363View ArticleGoogle Scholar
- Pusateri AE, McCarthy SJ, Gregory KW, Harris RA, Cardenas L, McManus AT, Goodwin CWJ: Effect of a chitosan-based hemostatic dressing on blood loss and survival in a model of severe venous hemorrhage and hepatic injury in swine. The Journal of Trauma 2003, 54: 177-182. 10.1097/00005373-200301000-00023View ArticleGoogle Scholar
- Rabea EI, Badawy MET, Stevens CV, Smagghe G, Steurbaut W: Chitosan as antimicrobial agent: Applications and mode of action. Biomacromolecules 2003, 4: 1457-1465. 10.1021/bm034130mView ArticleGoogle Scholar
- Sheng W, Wang G, La Pierre DP, Wen J, Deng Z, Wong C-KA, Lee DY, Yang BB: Versican mediates mesenchymal-epithelial transition. Mol Biol Cell 2006, 17: 2009-2020. 10.1091/mbc.E05-10-0951View ArticleGoogle Scholar
- Shi Y, Ma L, Zhou J, Mao Z, Gao C: Collagen/chitosan‐silicone membrane bilayer scaffold as a dermal equivalent. Polymers for advanced technologies 2006, 16: 789-794.View ArticleGoogle Scholar
- Tchemtchoua VT, Atanasova G, Aqil A, FileÌe P, Garbacki N, Vanhooteghem O, Deroanne C, As NÌ, JeÌrome C, Nusgens B, Poumay Y, Colige A: Development of a chitosan nanofibrillar scaffold for skin repair and regeneration. Biomacromolecules 2011, 12: 3194-3204. 10.1021/bm200680qView ArticleGoogle Scholar
- Thomas NW: Versican: A versatile extracellular matrix proteoglycan in cell biology. Curr Opin Cell Biol 2002, 14: 617-623. 10.1016/S0955-0674(02)00375-7View ArticleGoogle Scholar
- Ueno H, Mori T, Fujinaga T: Topical formulations and wound healing applications of chitosan. Advanced Drug Delivery Reviews 2001, 52: 105-115. 10.1016/S0169-409X(01)00189-2View ArticleGoogle Scholar
- Van den Bogaerdt AB, van Zuijlen PZ, van Galen MG, Lamme EL, Middelkoop EM: The suitability of cells from different tissues for use in tissue-engineered skin substitutes. Arch Dermatol Res 2002, 294: 135-142. 10.1007/s00403-002-0305-3View ArticleGoogle Scholar
- Wang X, Yu X, Yan Y, Zhang R: Liver tissue responses to gelatin and gelatin/chitosan gels. Journal of Biomedical Materials Research Part A 2008, 87A: 62-68. 10.1002/jbm.a.31712View ArticleGoogle Scholar
- Wu Y, Chen L, Zheng P-S, Yang BB: B1-integrin-mediated glioma cell adhesion and free radical-induced apoptosis are regulated by binding to a c-terminal domain of pg-m/versican. J Biol Chem 2002, 277: 12294-12301. 10.1074/jbc.M110748200View ArticleGoogle Scholar
- Yannas IV, Lee E, Orgill DP, Skrabut EM, Murphy GF: Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin. Proceedings of the National Academy of Sciences 1989, 86: 933-937. 10.1073/pnas.86.3.933View ArticleGoogle Scholar
- Zeltinger J, Sherwood JK, Graham DA, Mueller R, Griffith LG: Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. Tissue Eng 2001, 7: 557-572. 10.1089/107632701753213183View ArticleGoogle Scholar
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