European rodent on the edge: status and distribution of the Vojvodina blind mole rat
© Németh et al.; licensee Springer. 2013
Received: 24 October 2012
Accepted: 3 January 2013
Published: 4 January 2013
Recent research of blind mole rats of the species complex Nannospalax (superspecies leucodon) identified a small and fragmented population of these rodents on both sides of the Hungarian-Serbian border. Cytogenetic investigations proved that this population karyologically identical with the Vojvodina blind mole rat described earlier as Nannospalax (leucodon) montanosyrmiensis. Based on cytochrome b gene sequences obtained from three specimens originating from separate locations, these blind mole rats form a discrete phylogenetic clade which, with a difference of about 10%, is well separated from other blind mole rat taxa inhabiting the Carpathian Basin. The taxon has only two extant populations that are 150 km apart from each other. The combined occupied area is estimated to be less than 10 km2, and the total estimated number of individuals is less than 300. These two remaining populations are heavily fragmented and under imminent threat by the establishment of tree plantations, small-scale and agro-industrial farms and land development. The situation is further aggravated by the fact that 80% of the individuals inhabit unprotected areas. A study of the landscape history of the wider area surrounding one of the populations - based on military maps spanning over the last 200 years - has shown a drastic decrease in the extent and quality of potential habitats. Based on our present knowledge, the Vojvodina blind mole rat is one of the most seriously threatened, rarest mammal in Europe, the remaining population of which can be wiped out within years unless immediate conservation action is taken.
Rodents (Order Rodentia) are usually not in the focus of conservation biology (Lidicker 1989). Proper evaluation of the conservation status of rodent species is further complicated by the confusion that surrounds almost all levels of rodent systematics (Corti 2001). Well-established taxonomy is the base for efficient conservation biology, but unclear taxonomical questions can easily result in the negligence of certain groups, whose extinction would mean the loss of entire evolutionary lineages, drastically decreasing the overall biodiversity on Earth. The vulnerability of this order is demonstrated by the fact that rodent species contributed 51–52% to mammalian extinctions in the last 500 years (Ceballos and Brown 1995, MacPhee and Flemming 1999). Counterintuitively though, conservation initiatives will continue to be biased towards the most studied and attractive mammal groups and species (Amori and Gippoliti 2000).
The situation is clearly mirrored in the case of the Eurasian blind mole rats (Spalacidae: Spalacinae). These small mammals represent a distinct group among rodents which is extremely adapted to subterranean life. They have cylindrically shaped body with no external ear and a vestigial tail, and are completely blind spending their entire life in their tunnel system built underground (Topachevskii 1969). Compared to other rodents, the conditions resulting from their lifestyle created a decreased morphological variability and the species are very similar both externally and osteologically (Nevo 2000). Putting aside the lineage of large mole rats (genus Spalax) (for taxonomic context and nomenclatural details see Topachevskii 1969, Németh et al. 2009, Arslan, Akan and Zima 2011, Hadid et al. 2012), taxa belonging to Nannospalax present a long-standing source of dispute and disagreement on their systematics (Savić and Nevo 1990, Musser and Carleton 2005). Within the latter genus one of the recognised species groups (regarded as superspecies) which include a large number of karyologically different taxa (for the list of these named forms see Savić and Soldatović 1984) is Nannospalax (superspecies leucodon) (Musser and Carleton 2005). Molecular genetic investigations of this superspecies are quite limited so far both in terms of geographic and taxonomic coverage (Krystufek et al. 2011, Kandemir et al. 2012, Hadid et al. 2012) and the species status of taxa differentiated solely on chromosomal grounds have not been widely accepted (Sözen et al. 2006, Ivanitskaya et al. 2008). In accordance with the presently accepted view these taxa are herewith called “chromosomal forms”. Alongside with taxonomic uncertainty the determination of conservation status of different mole rat taxa is further hampered by their exclusively subterranean lifestyle which makes it difficult to evaluate their population size. While the leucodon-superspecies itself is categorised as Least Concern (Temple and Terry 2007), populations and habitats of many different European chromosomal forms are disappearing at an alarming rate, a phenomenon which has just recently been realized (Kryštufek and Amori, 2008; Németh et al., 2009).
In the course of researching mole rats of the Carpathian Basin, on both sides of the Hungarian-Serbian border a small and fragmented population of mole rats was identified in 2008. Cytogenetic investigations have proven that this Kelebia-Subotićka peščara population belongs to one of the four endemic chromosomal forms of the Carpathian Basin (Németh et al. 2009), Nannospalax (leucodon) montanosyrmiensis, described by Savić and Soldatović (1974) as Spalax montanosyrmiensis. This form was previously known only from two neighbouring localities at the foothills of Fruška Gora in Serbia (called as Stražilovo-Čortanovci population). In this paper we provide data for population size, distribution area and threatening factors of this poorly known chromosomal form.
Results and discussion
Due to the methodological difficulties to culture very limited amount of blood cells for cytology we were able to karyotype a single individual only. The karyotype of a mole rat found in the vicinity of Kelebia was 2n = 54 NF = 86 consisting of 2 pairs of metacentric autosomes, 8 pairs of submetacentric autosomes, 5 pairs of subtelocentric autosomes and 11 pairs of acrocentric autosomes; the X chromosome is large and metacentric, the Y chromosome is medium sized and acrocentric. This karyotype is identical with that of previously reported from the vicinities of Stražilovo and Čortanovci in Serbia and described as montanosyrmiensis (Savić and Soldatović 1974, Soldatović and Savić 1983).
Blind mole rat specimens used in the genetic analyses
Voucher accession no.
Recent populations of the Vojvodina blind mole rat
Area of occupancy (ha)
No. of individuals
Kelebia- Subotićka population
Kelebiai halastavak mellett
Stražilovo - Čortanovci population
Stražilovo - fallow
Stražilovo weekend houses
vicinity of Čortanovci
close to the Fruska gora- Čortanovci road
total population size
The Stražilovo-Čortanovci population (Figure 2b) is situated on the slopes of Fruška gora, almost 150 km south from the previous location. Clusters of mounds were found on pastures and meadows near Stražilovo and the population extends into the village of Čortanovci where one individual was caught in a kitchen garden. Blind mole-rats occur in a landscape mosaic of small woodlands, vineyards, orchards, pastures and meadows. Many of these cultivated habitat types have turned into spontaneously regenerating fallow land with elements of loess steppic vegetation. The actual habitat of the population is about 50 ha and the estimated population size is less than 100 individuals (Table 2). No part of the habitats of this population is protected.
The study area (10 × 10 km on the border of the settlements of Kelebia, Ásotthalom and Subotićka) encloses all presently known fragments of the Kelebia-Subotićka peščara population. Following the categorisation of the military surveys types of open and closed dry grasslands, dry pastures and dry hayfields are regarded herewith as potential mole rat habitats (see Topachevskii 1969, Savić and Nevo 1990, Horváth et al. 2007).
In the Hungarian part of the Kelebia-Subotićka peščara population the major threat presently is the change in land management: tree plantations, small-scale farming and agro-industrial farming. Following the afforestation of the area in 2007, the extent of the habitat of one of the sub-populations was reduced by 50%. A small fragment (numbering less than 20%) of the population falls into a Natura 2000 site (“Déli Homokhátság” SCI [Southern Sand Ridge SCI], code: HUKN20008), but the rest of the habitats are not protected by any means. In the Serbian part of the Kelebia-Subotićka peščara population the major threats are small-scale wood plantations. The whole area is protected as part of the Subotićka peščara Protected Area, however, despite this status serious habitat destruction took place in 2003.
In the Stražilovo-Čortanovci population the major threat is rapid habitat loss. Remaining grasslands and fallow lands are directly threatened. The field where the authors caught a specimen in 2009 was ploughed in 2010 wiping out all individuals on it. The subpopulations are isolated from each other and habitat fragments are under constant threat of transformation into cultivated land. Another obstacle to effective protection is that habitats are owned by a great number of proprietors, rendering long-term protection of the total population almost impossible. Presently, none of these areas are under any kind of protection.
The montanosyrmiensis form is therefore proposed to be ranked as Critically Endangered according to the IUCN (2001) criteria. This assessment is based on the extent of occurrence which is estimated to be less than 100 km2; area of occupancy is estimated to be less than 10 km2. The populations are severely fragmented in no more than two locations and continuing decline is observed in area, extent and quality of habitats.
This paper presents a case study where a mammal within Europe can drift to the brink of extinction almost unnoticed as a result of the lack of information, unclear taxonomic status and unrecognised tasks in conservation biology.
Karyotype of the blind mole-rat populations flanking the boundary of Serbia and Hungary were found identical to that of the montanosyrmiensis chromosomal form previously known only from Serbia (Soldatović and Savić 1983 ). The mtDNA analysis confirmed that the two populations form a discrete clade which is highly separated from other blind mole-rat taxa found in the Carpathian Basin. According to the results of the most comprehensive molecular biological research on blind mole rats based on 6 mitochondrial genes, the montanosyrmiensis form is a well separated lineage that diverged from the closest taxon examined about 1.8 million years ago (Hadid et al. 2012). Therefore, montanosyrmiensis complies with the criteria of the Genetic Species Concept (Baker and Bradley 2006), as well as the ESU (Evolutionary Significant Unit) (Moritz 1994).
Based on the surveys of all potential habitat patches in the presumed distribution area, the taxon montanosyrmiensis has only two extant populations that are 150 km apart from each other and the total estimated number of individuals is less than 300. Even these two remaining populations are heavily fragmented and extensive unsuitable vegetation types (forests, arable lands) between suitable habitat patches - often smaller than 3 hectares – possibly obstruct recent gene flow between subpopulations. The status and future of these population fragments are precarious as 80% of the individuals inhabits areas with no protection of any form, while constantly being threatened by factors such as ploughing, afforestation and site development. As the landscape history study revealed the Kelebia-Subotićka peščara population has lost more than 70% of its potential habitat in the last 200 years.
Based on our present knowledge, the Vojvodina blind mole rat is one of the most seriously threatened, rarest mammal in Europe, the remaining population of which can be wiped out within years unless immediate conservation action is taken.
Distribution and population size
Coordinates of all clusters of mounds were recorded by a hand-held GPS device. GPS records outlined the total extent of the distribution area. Spatial information, such as size and distribution of habitat patches, annual changes in the geographical position of mounds was tracked by means of Google Earth 5.1 and ArcGIS 9.0.
As the Vojvodina blind mole rat is strictly protected both in Hungary and Serbia only 1-3 specimens were caught alive per sub-populations to prove the presence of the animals as allowed by research permits issued by the National Inspectorate for Environment, Nature Conservation and Water no. 14/05173-3/2006 and 14/1840-3/2008. Altogether 16 animals were captured and handled in the field in accordance with guidelines approved by the American Society of Mammalogists (Gannon et al. 2007). The animals were caught by opening the tunnel system and capturing the animal trying to mend the damage (Németh et al. 2007). After samples were taken for tissue cultivation and DNA-analysis, individuals were released at the site of capture straight into their own tunnels. For cytogenetic investigations, instead of using the lethal direct bone marrow preparation (widely used in rodents) we took blood samples aided by veterinarians, applying topical and systemic aenesthesia and 70% alcohol disinfection following the protocoll described by Sós et al. (2009). Lymphocyte cultures were used for chromosomal investigations. Metaphase arrest, hypotonic treatment and chromosome preparation were carried out according to standard cytogenetic techniques (Moorhead et al.1960) with using RPMI 1640 medium containing 20% FCS, antimycotic, antibiotic solutions and the mixture of phytohaemagglutinin and pokeweed mitogen (1-1%).
The main objective of the molecular biological investigation was to set the Kelebia-Subotićka peščara population and the Stražilovo-Čortanovci population in genetic comparison. Genomic DNA was isolated from fresh tissue samples with Qiagen DNeasy Blood and Tissue kit (Qiagen, California, USA) according to the extraction protocol. The 866 bp long sequences of cytochrome b was amplified with PCR using forward F-muarso (5′-ATGACATGAAAAATCATYGTTGT-3′) and reverse R-muarso (5′-GAAATATCATTCKGGTTTAATRTG-3′) primer pairs. The polymerase chain reaction was performed in total volume of 25 μL containing 30 ng template DNA, 1 μM of each oligonucleotide primer, 1.5 mM MgCl2, 160 μM dNTPs and 0.5U AmpliTaq DNA polymerase (Applied Biosystems). PCR amplification was conducted in a DNA Engine Dyad (MJ Research) employing 94°C for 2 min followed by 45 cycles of 94°C for 45 sec, 48°C for 15 sec (ramp speed down to 48°C: 1C/sec), 60°C for 1 sec (ramp speed: 0.5C/sec up to 60°C) and 72°C for 2 min (ramp speed: 1C/sec up to 72°C), and the final extension step was 72°C for 7 min. The PCR-product was checked on 1.6% agarose gel stained with ethidium bromide and cleaned with Qiagen Clean Up kit (Qiagen, California, USA).
The A-tailing reaction was contained 7 μL of PCR product, 200 μM dATP, 1.5 mM MgCl2, 1x Taq buffer and 5U AmpliTaq DNA polymerase. The tailing profile was 94°C for 3 min and 70°C for 30 min. The A-tailed PCR product was ligated into pGEM Easy vector (Promega) and transformed into JM109 cells. Positive colonies were applied as a template for reamplification by PCR utilizing the T7 and SP6 primers. The products were checked on 1.6% agarose and then cleaned with SureClean (Bioline). The sequencing reaction was accomplished in GeneAmp PCR System 9700 machine according to the thermal profile of 94°C for 4 min followed by 25 cycles of 94°C for 30 sec, 50°C for 15 sec and 60°C for 4 min. The 10 μL sequencing reaction consisted of 80 ng template, 2 μL of BigDye v3.1 Terminator, 1 × buffer and 1 μM primer and then products were cleaned using the BigDye XTerminator Purification Kit. Sequences for both directions were obtained using ABI3130 Genetic Analyser and were aligned and tested in MEGA v5 (Tamura et al. 2011). Prior to the analysis the aligned sequences were checked for the presence of stop codons by using ORF finder at NCBI (http://www.ncbi.nlm.nih.gov/gorf/gorf.html). Phylogenetic relation of the samples was reconstructed using UPGMA method based on similarity matrix calculated with “no. of differences” (the number of different base-pairs between two compared sequence) and “kimura 2-parameter” technique. P-distance was obtained by dividing the number of nucleotide differences by the total number of nucleotides compared.
The landscape history could only be investigated on the two remaining populations of the Vojvodina blind mole rat but historical maps covering the last two centuries are available for the Kelebia-Subotićka peščara population only. These historical sources, which contain information on the changes of the landscape and of land use both in space and time, were the relevant digitized chart pages of military maps from the 1st (Anon. 1785) and 2nd (Anon. 1869) Military Mapping Survey of Austria-Hungary, and from the map series published by Honvéd Mapping Institute (Anon. 1959). Changes in land use during the past 50 years were assessed based on aerial photography (Anon. 2007). To track and interpret changes in landscape history ArcGIS 9.0 software was used.
IUCN classifications and categories
The Red List categories were assessed according to the 2001 criteria (IUCN 2001). Habitats and threats were classified (and terms used) according to the IUCN Habitats Classification Scheme 3.0 and Threats Classification Scheme 2.1, respectively (http://www.iucnredlist.org).
We thank the Hungarian and Serbian nature conservation authorities, especially Zoltán Vajda of Kiskunság National Park Directorate and Mihály Mikes, Bence Mikes, Ottó Szekeres and Jadranka Lada Delić of Institute for Nature Conservation of Serbia, for providing the necessary permits, and for their various support during our field work. Our special thanks are due to the late Ágnes Major for her tireless work and advices in the genetic investigations till the very last moments of her life. The project was partially financed by the Kiskunság National Park Directorate, the Ministry of Rural Development under project no: K-36-09-00182 W and supported by many ways by the Hungarian Natural History Museum.
- Amori G, Gippoliti S: What do mammalogists want to save? Ten years of mammalian conservation biology. Biodiv and Cons 2000, 9: 785-793. 10.1023/A:1008971823774View ArticleGoogle Scholar
- Anon: I. Military Mapping Survey of Austria-Hungary (1763-1785) 1:28.800 Digitized Map of The Hungarian Kingdom. Arcanum Kft, Budapest; 1785.Google Scholar
- Anon: II. Military Mapping Survey of Austria-Hungary (1806-1869) 1:28.000 Digitized Map of The Hungarian Kingdom and Temes Banat. Arcanum Kft, Budapest; 1869.Google Scholar
- Anon: Military Map of Hungary (1953-1959) 1:25.000. Honvéd Mapping Institute, Budapest; 1959.Google Scholar
- Anon: IKONOS 1 meter satellite image 2007. Hungarian Institute of Geodesy, Cartography and Remote Sensing, Budapest; 2007.Google Scholar
- Arslan A, Akan S, Zima J: Variation in C-heterochromatin and NOR distribution among chromosomal races of mole rats (Spalacidae) from Central Anatolia, Turkey. Mamm Biol 2011, 76: 28-35.Google Scholar
- Baker RJ, Bradley RD: Speciation in mammals and the Genetic Species Concept. J Mamm 2006, 87: 643-662. 10.1644/06-MAMM-F-038R2.1View ArticleGoogle Scholar
- Bradley RD, Baker RJ: A test of the Genetic Species Concept: Cytochrome-b sequences and mammals. J Mamm 2001, 82: 960-973. 10.1644/1545-1542(2001)082<0960:ATOTGS>2.0.CO;2View ArticleGoogle Scholar
- Ceballos G, Brown JH: Global patterns of mammalian diversity, endemism, and endangerment. Cons Biol 1995, 9: 559-568. 10.1046/j.1523-1739.1995.09030559.xView ArticleGoogle Scholar
- Corti M: Chromosomal speciation and phenotypic evolution in the house mouse. Biol J Linn Soc 2001, 73: 99-112. 10.1111/j.1095-8312.2001.tb01349.xView ArticleGoogle Scholar
- Gannon WL, Sikes RS, the Animal Care and Use Committee of the American Society of Mammalogists: Guidelines of the American Society of Mammalogists for the use of wild mammals in research. J Mamm 2007, 88: 809-823. 10.1644/06-MAMM-F-185R1.1View ArticleGoogle Scholar
- Hadid Y, Németh A, Snir S, Pavlíček T, Csorba G, Kázmér M, Major A, Mezhzherin S, Rusin M, Coşkun Y, Nevo E: Is evolution of blind mole rats determined by climate oscillations? PLoS One 2012., 7: 10.1371/journal.pone.0030043Google Scholar
- Horváth R, Bihari Z, Németh A, Csorba G: Nyugati földikutya. (Lesser blind mole rat). In Magyarország emlőseinek atlasza. (The Atlas of Hungarian mammals). Edited by: Bihari Z, Csorba G, Heltai M. Kossuth Könyvkiadó, Budapest; 2007:157-159. in HungarianGoogle Scholar
- IUCN: IUCN Red List Categories and Criteria: Version 3.1. IUCN, Gland, Switzerland; 2001.Google Scholar
- Ivanitskaya E, Sözen M, Rashkovetsky L, Matur F, Nevo E: Discriminiation of 2n = 60 Spalax leucodon cytotypes (Spalacidae, Rodentia) in Turkey by means of classical and molecular cytogenetic techniques. Cyt Genome Res 2008, 122: 139-149. 10.1159/000163091View ArticleGoogle Scholar
- Kandemir I, Sözen M, Matur F, Kankiliç T, Çolak F, Özkurt S, Çolak E: Phylogeny of species and cytotypes of mole rats (Spalacidae) in Turkey inferred from mithchondrial cythocrome b gene sequences. Folia Zool 2012, 61: 25-33.Google Scholar
- Kryštufek B, Amori G: Spalax leucodon. In IUCN Red List of Threatened Species Edited by: IUCN 2010. 2008. Version 2010.4. Accessed 7 November 2011 http://www.iucnredlist.org Version 2010.4.Google Scholar
- Kryštufek B, Ivanitskaya E, Arslan A, Arslan E, Buzan EV: Evolutionary history of mole rats (genus Nannospalax) inferred from mitochondrial cytochrome b sequence. Biol J Linn Soc 2011. 10.1111/j.1095-8312.2011.01795.xGoogle Scholar
- Lidicker WZ (Ed): Rodents. A World survey of species of conservation concern. 4th edition. Occ Papers IUCN Species Survival Commission. IUCN, Gland; 1989:60.Google Scholar
- MacPhee RDE, Flemming C: Requiem Æternam: The Last Five Hundred Years of Mammalian Species Extinctions. In Extinctions in Near Time: Causes, Contexts, and Consequences. Edited by: MacPhee RDE. Plenum Press, New York; 1999.View ArticleGoogle Scholar
- Moorhead PS, Nowell PC, Mellman WJ, Battips DM, Hungerford DA: Chromosome preparation of leukocytes cultured from human peripheral blood. Exp Cell Res 1960, 20: 613-616. 10.1016/0014-4827(60)90138-5View ArticleGoogle Scholar
- Moritz C: Defining evolutionarily significant units for conservation. Trends Ecol Evol 1994, 9: 373-375. 10.1016/0169-5347(94)90057-4View ArticleGoogle Scholar
- Musser G, Carleton M: Superfamily Muroidea. In Mammal species of the world: A taxonomic and geographic reference. 3rd edition. Edited by: Wilson DE, Reeder DM. The Johns Hopkins University Press, Baltimore; 2005:894-1531.Google Scholar
- Németh A, Czabán D, Csorba G, Farkas J: Evaluating the methods for live-trapping of the Hungarian lesser blind mole rat (Spalax leucodon). Term véd Közl 2007, 13: 417-421. in Hungarian with English abstractGoogle Scholar
- Németh A, Révay T, Zs H, Farkas J, Czabán D, Rózsás A, Csorba G: Chromosomal forms and risk assessment of Nannospalax (superspecies leucodon) (Mammalia: Rodentia) in the Carpathian Basin. Folia Zool 2009, 58: 349-361.Google Scholar
- Nevo E: Mosaic evolution of subterranean mammals: regression, progression, and global convergence. Oxford University Press, Oxford; 2000:413.Google Scholar
- Savić I, Nevo E: The Spalacidae: Evolutionary history, speciation, and population biology. In Evolution of subterranean mammals at the organismal and molecular levels. Edited by: Reig AO, Nevo E. Alan R. Liss, New York; 1990:129-153.Google Scholar
- Savić I, Soldatović B: Die Verbreitung der Karyotypen der Blindmaus Spalax (Mesospalax) in Jugoslavien. Arhiv Biol Nauka 1974, 26: 115-122. (in German)Google Scholar
- Savić I, Soldatović B: Karyotype evolution and taxonomy of the genus Nannospalax Palmer 1903, Mammalia, in Europe. Separate edition of the Serbian Academy of Science and Arts. Beograd 1984, 59: 104.Google Scholar
- Soldatović B, Savić I: New data on distribution of the representatives of the genus Nannospalax Palmer, 1903, in SR Serbia. Proc Second Symposium on Fauna of SR Serbia, Beograd; 1983:171-174.Google Scholar
- Sós E, Molnár V, Révay T, Németh A, Farkas J, Hidas A, Csorba G: Veterinarian participation at the critically endangered lesser blind mole rat (Nannospalax (superspecies leucodon)) research in Hungary. In Proceedings of the International Conference on Diseases of Zoo and Wild Animals. Edited by: Wibbelt G, Kretzschmar P, Hofer H, Seet S. Beekse Bergen, The Netherlands; 2009:118-121.Google Scholar
- Sözen M, Matur F, Çolak E, Özkurt S, Karatas A: Some karyological records and a new chromosomal form for Spalax (Mammalia: Rodentia) in Turkey. Folia Zool 2006, 55(3):247-256.Google Scholar
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol 2011, 28: 2731-2739. 10.1093/molbev/msr121View ArticleGoogle Scholar
- Temple HJ, Terry A: The Status and Distribution of European Mammals. Office for Official Publications of the European Communities, Luxembourg; 2007:48pp.Google Scholar
- Topachevski VA: Fauna of the USSR: Mammals. Mole rats, Spalacidae. Smithsonian Institution and the National Science Foundation, Washington; 1969.Google Scholar
- Zuri I, Terkel J: Summer tunneling activity of mole rats (Spalax ehrenbergi) in a sloping field with moisture gradient. Mammalia 1997, 61: 47-54.View ArticleGoogle Scholar
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