Effect of dietary flaxseed oil level on the growth performance and fatty acid composition of fingerlings of rainbow trout, Oncorhynchus mykiss

In the course of just a few decades, fish farming has developed into a highly productive and efficient industry to produce animal protein for human consumption. In addition to good growing conditions, a prerequisite for productivity and economic sustainability in fish farming can be a reliable supply of effective feeds. For various reasons, fish meal and fish oil have historically been the dominant raw materials in the production of fish feeds. Due to the development of more energy dense feed types as well as general growth of the aquaculture industry, a significant proportion of the total global fish oil is used for its feed preparation. A lipid requirement equal to 100% of the world’s total fish oil production is estimated by the year 2010 (New, 1999).

While marine oils are superior in their fatty acid composition they also contain a variety of toxic compounds including polychlorinated dibenzo-p-dioxins (PCDD), polychlorinated dibenzofurans (PCDF) and dioxin-like polychlorinated biphenyls (DL-PCB), particularly the non-ortho and mono-ortho substituted PCBs (Jacobs et al., 2002a b; Hites et al., 2004a b). These compounds are suspected to be carcinogenic and immunosuppressive in humans (Birnbaum and Tuomisto, 2000; Baccarelli et al., 2002; Van Den Heuvel et al., 2002).

It is also well-known that lipid oxidation is one of the major concerns in fish-derived food products. Polyunsaturated fatty acids (PUFAs) are more easily oxidized than saturated fatty acids (SFAs), and therefore, food products enhanced with the PUFAs n-3 are also more prone to lipid oxidation. There is potential human health risks associated with increased consumption of oxidized PUFAs n-3 products (Fritsche and Johnston, 1990; Kubow, 1993). Another important factor to limit a more common use of PUFAs n-3 enhanced food products is the development of off-flavors following lipid oxidation that may be offensive to consumers (Waagbø et al., 1993).

While it is obvious that a substitute must be found, replacing fish oil in diets has its own difficulties as most of the vegetable oils are relatively poor sources of n-3 fatty acids. Exceptions to this are flaxseed and canola oils which are rich in alpha linolenic acid (18:3n-3) (53% and 12%, respectively) (NRC, 1993). However, these oils are devoid of longer chain n-3 highly unsaturated fatty acids (HUFAs n-3) and their inclusion in trout diets results in a significant decrease in the tissue levels of eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) (Bell et al., 2002 2003a). Moreover, enhancement of omega-3 fatty acid content in rainbow trout fillet was observed in farmed rainbow trout and brook trout as results of flaxseed oil inclusion in diet (Chen et al., 2006; Simmons et al., 2011).

Freshwater fish are capable of converting C18 PUFAs to the longer chain C20 and C22 PUFAs (Henderson and Tocher, 1987) which are the functionally essential fatty acids in vertebrates (Lauritzen et al., 2001).

Several studies conducted on freshwater fish indicated that vegetable oils can successfully replace fish oil in the feed without affecting their survival and growth (Wonnacott et al., 2004; Subhadra et al., 2006). Caballero et al., (2002) reported that in rainbow trout (Oncorhynchus mykiss) up to 80–90% of vegetable oils e.g. soybean, rapeseed, olive, and palm oils can be used without compromising fish growth. It has also been reported that partial replacement of fish oil by vegetable oils such as rapeseed, soybean, flaxseed or palm oils in fish feeds had no negative impacts on growth and survival of Atlantic salmon (Salmo salar) (Rosenlund et al., 2001), brook char (Salvelinus fontinalis) (Guillou et al., 1995), gilthead sea bream (Sparus aurata), European sea bass (Dicentrarchus labrax) (Izquierdo et al., 2003) and rainbow trout (Greene and Selivonchick, 1990; Caballero et al., 2002).

The aim of the present study was to evaluate the effects of fish oil replacement with flaxseed oil (relatively easily obtained and low priced oil) on growth, feed conversion ratio and fillet fatty acid composition of fingerlings of rainbow trout.

270 rainbow trout fingerlings with a mean initial body weight of 16.5 ± 0.5 g were purchased from Cheshmeh Dimeh fish hatchery (Shahre kord, Chaharmahal and Bakhtiari, Iran) and used in this study. Prior to the start of the experiment the fish were acclimatized to the new environmental conditions and the commercial diet (SFT2 of Chineh feed production factory, Tehran, Iran) for a two week period within a semi re-circulating system.

The results of the present study suggest that flaxseed oil can be used to replace fish oil without adverse effects on growth performance of rainbow trout fingerlings, as reported in other studies (Montero et al., 2005; Bell et al., 2004; Izquierdo et al., 2005). This was evidenced by the weight gain and feed conversion ratio which ranged from 246.7 ± 10.8% to 260.1 ± 4.2% and 0.90 ± 0.17 to 1.01 ± 0.05, respectively, with no significant differences from fish fed all experimental diets.

In agreement with previous studies (Caballero et al., 2002; Martino et al., 2002; Glencross et al., 2003; Turchini et al., 2003b), considerable differences were evident in the fatty acid composition of fish fed different lipid sources. For example, there was a high increase in the levels of α-linolenic acid in fish fed either with FxOD and/or FFxOD. As reported by other researchers, (Turchini et al., 2003a b; Guillou et al., 1995; Bell et al., 2003a b; Torstensen et al., 2004; Chen et al., 2006; Chen et al., 2008; Simmons et al., 2011), a high correlations are also exist between the individual fatty acids as well as MUFAs and PUFAs of a diet and the fish fillet (Table 6). There was, however, a high correlation between the amount of SFAs in the diet and SFAs in the fillet, which was not in accordance with the findings of Turchini et al., (2003a b) who postulated that SFAs were not used efficiently by Murray cod (Maccullochella peelii peelii) as an energy source and were subsequently deposited at an optimal level in preference to the other major fatty acid classes.

It is well known that freshwater fish have a dietary requirement for n-3 and n-6 fatty acids, predominantly in the form of α-linolenic and linoleic acids (Kanazawa et al., 1979 1980; Guillou et al., 1995; Martino et al., 2002; Izquierdo et al., 2003; Tocher, 2003). In comparison to marine fish species, freshwater fish are also generally better adapted to desaturate and elongate these base fatty acids to higher homologs (Guillou et al., 1995; Tocher, 2003). This study observed α-linolenic acid in lower concentrations in the muscle than in the diets. It is therefore suspected that a high degree of metabolism of this fatty acid for β-oxidation and/or desaturation and elongation is taking place in fingerlings of rainbow trout (O. mykiss). This is further bolstered by the presence of n-3 desaturation and elongation enzyme products in the form of 18:4n-3 and 20:3n-3 in fish fed FxOD and FFxOD. These fatty acids were found in much lower concentrations in the diets. Likewise, fish fed the FxOD and FFxOD contained n-6 desaturation and elongation intermediates (18:3n-6 and 20:3n-6) and indicate an elongation and desaturation of linoleic acid via Δ6 desaturase. However, further desaturation of 20:3n-6 to 20:4n-6 and 20:3n-3 to EPA and ultimately DHA was shrouded by high concentrations of these fatty acids within the fillet of initial fish samples. The Department of Health of England (Committee on Medical Aspects of Food Policy 1994) recommends a minimum PUFAs/SFAs ratio of 0.45, and a maximum n-6/n-3 of 4.0. Table 5 shows that our fish in all treatments met the PUFAs/SFAs and n-6/n-3 ratios. Despite the decrease in EPA and DHA in fillet from fish fed FFxOD, the trout fillets contained a relatively rich source of these fatty acids (584 mg of EPA plus DHA) with a 200 g serving portion of the fillets from fish fed FFxOD. This meets the intake of 500 mg day⁻¹ of EPA plus DHA recommended by the International Society for the Study of Fatty Acids and Lipids (Simopoulos et al., 1999).

Present study showed the substitution of fish oil with flaxseed oil in the rainbow trout (O. mykiss) diet have been possible without any negative effects on the growth and feed conversion ratio. However, the reflection of the dietary oil source on the fillet fatty acid composition of the fish could be a potential drawback for vegetable oil substitution from a human nutritional point of view, given the decreases in levels of EPA and DHA in fish fed the vegetable oil diets. Further investigation into the benefits of other vegetable oils or indeed a blend of various vegetable oils is required in order to reduce usage of traditionally used fish oils, while simultaneously avoiding a reduction in the human health protective properties found within fish flesh.