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Molecular linkage tracing of HIV-1 transmission events in seroconcordant couples in Guangxi Province, Southeastern China
SpringerPlus volume 5, Article number: 2015 (2016)
Guangxi Province in Southeastern China has one of the highest HIV-1 infection and transmission rates in stable couples. However, the mode of transmission at the molecular level has seldom been reported amongst this group. It is important to investigate this issue to support the treatment-as-prevention approach and for efficient interventions.
HIV-1 subgenomic regions (1.2 kb of pol and a 660-bp env C2V5 fragment) were sequenced in 42 couples. A couple linkage assessment was performed by phylogenetic analysis of sequences and Bayesian analysis of genetic distances. A subset of pairs was selected for single-genome amplification.
Thirty-five pairs (83.3 %, 35/42) were identified as linked, 3 pairs (7.1 %, 3/42) were identified as indeterminate, and 4 pairs (9.5 %) were identified as unlinked. The predominant intra-couple-transmitted HIV-1 subtype was CRF01_AE (80 %, 28/35). The median genetic distance of linked couples was 0.5 %.
The majority of HIV-1 transmission events in this study occurred within the partnership, and the predominant HIV-1 subtype was CRF01_AE. Further research on the mode of HIV transmission in other locations is needed.
Guangxi Province in China has a particularly high prevalence of HIV-1 infection. The complexity of the circulating viral subtypes and the high frequency of infection events in this region have placed a considerable economic burden on the government and posed great challenges to the HIV-1 disease control systems (Feng et al. 2013; Zeng et al. 2012). According to national and local molecular epidemiology reports, commercial heterosexual intercourse and intravenous drug use are two major routes of HIV-1 transmission in Guangxi. For infected people, their seronegative stable sexual partners are at a high risk of infection (He et al. 2012; Li et al. 2013). Two retrospective observational cohort studies performed in China showed that anti-retroviral therapy can reduce the seroconversion rate of HIV-1 seronegative partners; however, no molecular analysis was presented in these reports (Jia et al. 2012; Tang et al. 2015). Therefore, the possibility that seroconverters may have been infected from outside of the stable sexual partnership cannot be excluded. Our study aimed to investigate the mode of HIV transmission in heterosexual couples living in Guangxi using molecular methods to determine the optimum treatment programmes.
To improve the reliability of our results, both the HIV pol and env genes were analysed in this study (English et al. 2011; Sturmer et al. 2004). The phylogenetic linkage of HIV viruses from couples in Guangxi was analysed using genetic distance and Bayesian methods. The final results from linkage analysis were confirmed using single-genome amplification (SGA) on a subset of the population examined (Boily et al. 2010; Eyer-Silva and Morgado 2006; Jennes et al. 2012).
Sample sources and data collection
From July 2006 until April 2013, participants at voluntary counselling and testing (VCT) centres in Guangxi that tested positive for HIV-1 during screening donated plasma and whole blood samples for routine HIV-1 diagnostic assays. If they had stable partners, the individuals and their seronegative cohabitating partners had follow-up interviews and were tested at the same time. If the negative partners experienced seroconversion during the follow-up, HIV diagnostic assays and epidemiological data were collected into a serodiscordant database (Table 1, Jia et al. 2012). If the partners were already seropositive at the first follow-up, then the possible transmission time and direction were inferred by separate questionnaires, and these couples were entered into a seroconcordant database (Table 2). In this study, plasma samples obtained from routine deposits at the Guangxi Center for Disease control (CDC) were screened and selected.
The study was approved by the Institutional Review Board of the National Center for AIDS/STD Control and Prevention. Informed consent was obtained from all participants at the time of sample collection.
Viral RNA (vRNA) isolation, amplification and sequencing
Total viral RNA was extracted from 280 µl of thawed plasma using the Qiagen Viral RNA Mini Kit according to the manufacturer’s instructions (Qiagen, Germany). The fragment spanning the HIV-1 pol gene region (1.2 kb nucleotides in length), encoding all 99 amino acids in HIV protease and the first 317 amino acids in HIV reverse transcriptase, was amplified by one-step reverse transcription polymerase chain reaction (RT-PCR) (Phan et al. 2015). Considering the relatively high diversity of the envelope region, C2V5 sequences (HXB2 coordinates: 7002–7663) were amplified using near-limiting-dilution PCR. PCR products were purified and directly sequenced to keep the ambiguous base rate below 0.3 %. Technicians were blinded to specimen partnerships and conducted analyses on the index and partner samples in different rooms to minimize the risk of specimen contamination (Etemad et al. 2015). Additional details regarding the laboratory methods have been described in a previous report (Campbell et al. 2011).
Genotyping HIV-1 sequences by phylogenetic tree analysis
All pol and env sequences were aligned with HIV-1 reference subtypes (A–D, F–H, J, and K) obtained from the Los Alamos HIV Database (http://hiv-web.lanl.gov/) using MUSCLE in MEGA 5 software. Aligned columns with more than 50 % gaps were deleted by Gapstrip. The phylogenetic model was determined using the best-fit model estimated by FindModel (http://hiv-web.lanl.gov/). Maximum-likelihood trees were constructed with MEGA 5 software (Tamura et al. 2011) and bootstrap values were calculated from 500 replicates.
Phylogenetic linkage analysis of couple sequences
Maximum-likelihood trees of pol and env sequences were constructed using a general time reversible (GTR) nucleotide substitution with a gamma distribution of rates. To assure that the cluster represented the real relationship as much as possible, unrelated sequences from a local area (53 pol seq and 54 env seq) were added as controls and additional criteria were utilized, as reported in a previous study (Trask et al. 2002). We considered cluster bootstrap values ≥80 % to be indicative of genetic linkage and bootstrap values <80 % to be indeterminate, which were further analysed by pairwise genetic distance; we considered sequences separated in different branches as genetically unlinked (Jennes et al. 2012). According to our criteria, a couple with either of the two HIV genes (cluster bootstrap value >80 %) was determined to have a linked transmission relationship. The corresponding pairwise genetic distances of couples were calculated separately using MEGA 5 software, with a transition/transversion ratio of 0.5.
Bayesian analysis of genetic distances
We used a Bayesian algorithm to derive an estimate of the probability of the linkage between sequences in the cohort by pairwise genetic distance distribution, as previously reported. According to kernel density estimation, we calculated conditional densities of the genetic distances [f(X|linked)] from couples’ sequences in the serodiscordant cohort, which are epidemiologically and phylogenetically linked (linked training). Using the same method, we calculated f(X|unlinked) from single partner sequences in the control dataset (unlinked training). An empirical Bayes’ approach was used, and the initial value for P (linked) was 0.5; the posterior probabilities of linkage for the seroconcordant cohort was based on the linked and unlinked training data (more details in Additional file 1: Figure S1). The posterior probability of the linkage for each sequence pair, i, is given by Bayes’ formula:
Analysis of HIV env by single-genome amplification
Single-genome amplification was performed in five undetermined or unlinked couples to investigate the linkage relationship between the viral quasispecies of the partners to provide additional evidence to support our results (Haaland et al. 2009; Salazar-Gonzalez et al. 2009). vRNA was extracted and reverse-transcribed to generate cDNA, which was diluted to an optimum concentration to guarantee less than 20 C2V5 env gene PCR products in one reaction well (Salazar-Gonzalez et al. 2009). Phylogenetic trees of each couple were generated by the maximum-likelihood method using the SGA sequences to investigate the topology of paraphyly of the transmitted source (Scaduto et al. 2010).
Subject characteristics and sequence amplification
For this study, we collected 154 frozen serum samples from the Guangxi CDC’s couple library. Additional details on the subject demographic characteristics are provided in Additional file 2: Table S1. Amongst these samples, there were 50 pair samples and 54 single partner samples. Serodiscordant couple samples were collected around the time of seroconversion. Seroconcordant couple samples were selected at the first time of the index partners’ diagnosis. The sample set represented several geographically distinct areas of Guangxi.
Forty-one pol pair sequences had both partners’ samples, including 20 couples from the serodiscordant cohort and 21 couples from the seroconcordant cohort (Additional file 2: Table S1). Thirty-two env pair sequences were obtained as follows: 11 were from serodiscordant couples and 21 were from seroconcordant couples. Another 53 pol and 54 env sequences were obtained from a couple’s cohort in Guangxi Province, but their partners’ samples were not supplied or they experienced amplification failure; therefore, these samples were used as controls to represent the local circulating virus.
The CRF01_AE subtype highly correlates with most couples in Guangxi
A total of 118 env sequences and 135 pol sequences were obtained from this survey. According to the genotype results from the HIV database reference assignment, the major HIV-1 subtype was CRF01_AE (81.5 %, 110/135), followed by subtypes CRF08_BC (12.6 %, 17/135), CRF07_BC (4.4 %, 6/135) and URFs (1.5 %, 2/135) (Fig. 1). This is consistent with a multicentre molecular epidemiological observation in which subtype CRF01_AE was predominant in HIV patients infected via sexual transmission (Li et al. 2013).
Most HIV infections in surveyed couples are genetically linked
Genetic linkage was evaluated through phylogenetic tree analysis on the conserved pol and highly variable env C2V5 viral genes in each couple. A total of 41 pol sequence pairs and 32 env sequence pairs were obtained. Amongst these, 31 couples had both pol and env sequence pairs. Sequence pairs from 30 of 33 couples infected with CRF01_AE were very closely related and clustered in exclusive branches with high bootstrap values in the pol phylogenetic trees (Fig. 2). The median pairwise distance of these couples in the pol region was 0.008, indicating that they share a closely related CRF01_AE viral ancestor. Sequence pairs from two couples infected with CRF07_BC and three couples infected with CRF08_BC that clustered with high bootstrap values were defined as linked. Sequences from two other couples (GXDY1034-2034 and GXDY1294-2294) that clustered with low bootstrap values and one couple (GXDY1057-2057) with a pair sequence that was separated into a different branch were defined as unlinked (for details see Additional file 2: Table S1).
For the env region, 21 CRF01_AE couples and five CRF_07BC/08BC couples were clustered with high bootstrap values, including another linked couple (GXDY1057-2057) that lacked pol sequences. The median pairwise distance of 21 CRF01_AE couples in the env region was 0.05 (Fig. 3).
In conclusion, 35 pairs (85.3 %, 35/41) in the pol phylogenetic tree and 26 pairs (81 %, 26/32) in the env phylogenetic tree were defined as linked by monophyly. Thirty-one pairs had both gene sequences. Amongst them, there were 25 pairs (80 %, 25/31) with linkage in both genes and four pairs were separated phylogenetically (GXDY1057-2057, GXDY1223-2223, GXDY1294-2294, and GXDY1307-2307).
Genetic distance and Bayesian analyses
The median pairwise genetic distance was 0.5 % (range 0.0–4.1 %) in pol and 4.0 % (range 0.0–11.7 %) in env. In unlinked and indeterminate pairs, the genetic distance was 2.0 % (range 0.4–5.0 %) and 12 % (range 8–16 %) in pol and env, respectively. Median Bayesian posterior probabilities for linked (n = 17) and unlinked (n = 6) pairs in seroconcordant couples (n = 23 pairs) were 100.0 and 0.0 % in pol and 99.8 and 9.0 % in env (Additional file 2: Table S1), respectively.
In one couple (GXDY 1307–2307), the sequence pair was monophyletic, and posterior probabilities were high for pol (100.0 %) but low for env (9 %). The results from the phylogenetic and Bayesian analyses were inconsistent in three paired couples (GXDY1290–2290, GXDY1293–2293 and GXDY1294–2294) with respect to pol and one pair (GXDY1293–2293) with respect to env.
Paraphyly was used to identify linked couples in the phylogenetic tree
We evaluated single env sequences in five pairs showing inconsistencies either between the two genes or between the two analytical methods used (GXDY1057–2057, GXDY1223–2223, GXDY1293–2293, GXDY1294–2294, and GXDY1307–2307). One pair (GXDY1290–2290) was classified as indeterminate for a lack of env SGA sequences.
Results from the linked couples showed that sequences from the donors were paraphyletic with respect to the recipients’ sequences, as expected (Scaduto et al. 2010). For example, in the couple GXDY1223–2223, the GXDY2223 sequences were clustered with a small proportion of GXDY1223 sequences as monophyly (Fig. 4). This phenomenon suggests that the genetic distance of some quasispecies sequences from the donor and recipient are close enough to mingle with each other.
To accomplish the 2030 UN goal to end the HIV epidemic, new HIV-1 transmission should be controlled and the potential transmission source identified, especially in stable couples (Cousins 2016). In the famous HPTN 052 prospective research, robust evidence proved the efficiency of the treatment-as-prevention strategy (TAPS) after removing outside transmission couples (Eshleman et al. 2011). The sustained decrease in genetically-linked transmission in couples has been further proven in a newly published HPTN052 report (Cohen et al. 2016). Considering the huge population in China, the TASP approach has great significance in public health. A national retrospective analysis using epidemic data supports the HPTN052 conclusion (Jia et al. 2012), which is the same conclusion made in a recent study in Henan Province (Smith et al. 2015). A recent epidemiological investigation of serodiscordance in Guangxi again supported the efficiency of TASP. However, not one report has investigated the local transmission relationship at the molecular level in China. Here, we provide molecular evidence to complement the related research.
Phylogenetic analyses of suspected transmission relationships have been widely used to provide evidence and clues for many HIV-1 transmission events (Blanchard et al. 1998; Kim et al. 1999; Leitner et al. 1996; Lopes et al. 2015). Unlike previous reports of seroconcordant couples (Jennes et al. 2012), Guangxi Province has the following characteristics that render the identification of the viral transmission relationship challenging (Feng et al. 2013): an influx of a large number of individuals infected with different CRF01_AE lineages, a high rate of HIV-1 infection amongst drug users, and cross-infection amongst different risk groups that provides opportunities for viral recombination. This can be shown from the scale bar presenting the pol phylogenetic tree and one couple infected by a unique recombinant form (pair no. 299) (Wang et al. 2013). In our study, to identify the true relationship of these couples, phylogenetic analysis of two subgenomic regions combined with epidemiological data were used to determine the linkage status. Some pair sequences were further analysed phylogenetically using SGA to validate the results.
We identified 83.3 % (35/42) of intra-couple infections in our sample that were linked and 9.5 % (4/42) that were unlinked. The majority of cases were classified using phylogenetic analysis by bulk PCR. However, for the special cases in which the non-dominant quasispecies of the donor were transmitted or the viral variants of the recipient evolved with selection, the inference made by one or two sequences from couples could not reveal the true relationship. For example, the pol gene of pair no. 057 indicated unlinked transmission, which was inconsistent with the env gene results. From the SGA characterisation of the couple’s quasispecies, we found two distinct groups of progeny virus in the donor, and only the non-dominant variant was transmitted to his partner. Thus, viral diversity may complicate linkage analysis if the sequences chosen from the index and partner belong to two different dominant viruses (Sturmer et al. 2004).
Although there are many epidemiological studies on serodiscordant and seroconcordant couples in China, they are all based on the assumption that intra-couple transmissions are linked. For the first time, this cross-sectional study demonstrates the true relationship of the serodiscordant and seroconcordant couples in the local area using molecular techniques. In this study, we used the standard combination method to investigate the viral relationship of the couples and to learn about the genetic distance distribution of linked pairs.
In comparison with studies from other countries, all the data in this survey showed a comparable intra-family transmission rate in Guangxi, and the main HIV-1 infection source was extramarital occasional sex (Eshleman et al. 2011; Jennes et al. 2012; Trask et al. 2002). The predominant HIV-1 subtype in our sample set was CRF01_AE, which is mainly transmitted through sexual routes and has the characteristics of faster clinical progression than other subtypes (Li et al. 2014). All of these factors support the importance to initiate ART early as a preventative method in Guangxi serodiscordant couples to reduce intra-family sexual transmission.
There are several limitations to this study. First, the sample size is small and spans a long period of time. In future studies, we will enrol more participants from multiple centres. Secondly, the transmission direction of seroconcordant couples who lacked epidemic data were obtained from questionnaires; in future studies, proper mathematic models should be used to identify the direction (Yang et al. 2012). Lastly, this is a small-scale study using convenient samples. Future studies with larger sample sizes are warranted.
In conclusion, this is a comprehensive analysis that aimed to identify the mode of intra-family HIV transmission. The data presented here provide evidence of a transmission source. Further research into the serodiscordant and seroconcordant couples in Guangxi and other regions in China will provide more information about intra-family HIV transmission and will aid in the design of related intervention policies in this region.
Nucleotide sequences of GXDY pol and GXDY env regions have been submitted to GenBank under accession numbers KF835116–KF835250 and KF834998–KF835115.
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NW performed all experiments, data analysis and drafted the manuscript. ZT provided the samples and corresponding epidemiological data. YL revised the manuscript. PX participated in the statistical analysis. YS participated in the study design and coordinated the process in its entirety. All authors read and approved the final manuscript.
This research was supported by the National Major Projects for Infectious Disease Control and Prevention (Grants 2012ZX10001008 and 2012ZX10001002), the National Natural Science Foundation of China (Grant 81020108030), the State Key Laboratory for Infectious Disease Development (Grant 2012SKLID103), and Guangxi Bagui Honor Scholars.
The authors declare that they have no competing interests.
Nidan Wang and Zhenzhu Tang contributed equally to this work
Additional file 1: Figure S1. Flow chart depicting the process of screening our sample set and 339 determining genetic linkage and direction of transmission.
Additional file 2: Table S1. Epidemiological analysis and conclusion of HIV-1 transmission linkage for 42 HIV-1 couples.
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Wang, N., Tang, Z., Li, Y. et al. Molecular linkage tracing of HIV-1 transmission events in seroconcordant couples in Guangxi Province, Southeastern China. SpringerPlus 5, 2015 (2016). https://doi.org/10.1186/s40064-016-3578-2
- Serodiscordant couples
- Seroconcordant couples
- HIV-1 transmission
- Phylogenetic analysis, Bayesian analysis