Effects of exogenous enzymes and application method on nutrient intake, digestibility and growth performance of Pelibuey lambs
© The Author(s) 2016
Received: 12 April 2016
Accepted: 15 August 2016
Published: 23 August 2016
Pelibuey sheep is the main breed in the tropical and subtropical regions of Mexico, and high demand of sheep meat has favored the finishing of lambs in feedlots with diets containing high levels of grains. The objective of this study was to evaluate the effects of exogenous enzymes (EE) and application method on nutrient intake and digestibility and performance of growing Pelibuey lambs. Treatments were based on comparison of two different methods of adding an enzyme product (sprayed on the total mixed ration or applied orally to the lambs) versus control treatment (no added enzyme). Twenty-one Pelibuey lambs, weighing 15.7 kg (SD = 1.8 kg) initial body weight, were individually housed in shaded pens and assigned randomly to one of the three enzyme treatments. At the end of study (lasting for 45 days), three lambs from each treatment were randomly selected and adapted to a pants and harness designed for fecal collection to measure nutrient digestibilities. Total body gain and average daily gain were affected (P < 0.05) by supplemental EE. The application method of EE had significant (P < 0.05) effect on FCE and FCR, but no effects were observed on nutrient intake. Supplemental EE did improve (P < 0.05) the digestibilities of dry matter, organic matter, neutral and acid detergent fiber, but no differences were observed in crude protein digestibility. The application method of EE had significant (P < 0.05) effect on the digestibility of acid detergent fiber. Supplemental EE can improve body weight gain and nutrient digestibilities without affecting nutrient intake in Pelibuey lambs, but the results of feed conversion efficiency and acid detergent fiber digestibility depend on the application method used of the EE.
Pelibuey sheep is the main breed in the tropical and subtropical regions of Mexico, probably due to exceptional fecundity and adaptability to heat, humidity, parasites, scarcity of feed and other harsh environment conditions. High demand of sheep meat has favored the exploitation of Pelibuey sheep in the northeast dry tropic of México with breeding stock under grazing conditions and growing lambs finished in feedlots with diets containing high levels of grains. Inclusion of high levels of grain in the diet for ruminants has been associated with declining rates of fiber digestion due to lower fibrolytic activity in the rumen (Martin and Michalet-Doreau 1995; Noziere et al. 1996) and an apparent microbial preference for non-structural carbohydrates (Mould and Ørskov 1983). This may create conditions in which supplementary exogenous enzymes (EE) can have beneficial effects.
The beneficial impact of addition of EE depends on several factors such as diet composition, type of enzyme preparation, enzyme stability, specific enzyme activities, amount of enzyme added and application method (Yang et al. 2000; Morgavi et al. 2001; Wallace et al. 2001). Applying fibrolytic exogenous enzymes in a liquid form to feeds prior to consumption can have a positive effect on animal performance (Rode et al. 1999; Yang et al. 2000). The close association of enzymes with feed may enable some form of pre-ingestive attack of the enzymes upon the plant fiber and enhance binding of the enzymes to the feed. Kung et al. (2000) emphasized the importance of interaction time, since enzyme addition to feeds may create a stable enzyme-feed complex that protects free enzymes from proteolysis in the rumen, but other authors have indicated an alteration in fiber structure, which would stimulate microbial colonization (Nsereko et al. 2000). In contrast, direct infusion of enzyme into the rumen is far less effective or can even reduce digestibility of forages compared with application of liquid enzyme to substrate (Lewis et al. 1996). Yang et al. (2000) reported increased milk production and digestibility of the diet when enzymes were added to the concentrate portion of a dairy cow diet, but not when they were added directly to total mixed ration. The objective of this study was to evaluate the effect of cellulases exogenous enzymes and application method on nutrient digestibilities and performance of growing Pelibuey lambs.
Animals and diets
The study was conducted at an experimental farm of Facultad de Ingeniería y Ciencias-Universidad Autónoma de Tamaulipas, located in northeast Mexico (23°56′N, 99°06′W), at 190 m above sea level. The climate is considered dry tropic, which is semi-arid and sub-humid, with summer rains and sporadic winter rains. The average annual temperature is 23 °C with a total annual rainfall of 800 mm. All procedures involving the animals followed the international guiding principles.
Ingredients and chemical composition (g/kg DM) of basal diet of buffel grass fed to growing male Pelibuey lambs
Buffel grass hay
Metabolizable energy (Mcal/kg)
Neutral detergent fiber
Acid detergent fiber
Enzyme product and application methods
TMR fed ad libitum with no added enzyme (Control).
Control TMR diet plus enzyme product (TMR-E). 1 ml of enzyme product was diluted in 10 ml of distilled water, and 10 ml of diluted enzyme were added per kg of TMR DM. This was equivalent to 1 ml of enzyme product per kg of TMR DM. Diluted enzyme solution was sprayed on the TMR and mixed daily 24 h before morning feeding.
Control TMR plus enzyme product applied orally (Oral-E). The enzyme product was diluted as explained in the TMR-E treatment but the solution was given orally to each lamb using a syringe 1 h before the morning feeding. The daily dose of the diluted enzyme product was applied at 10 ml per kg of TMR DM. The dosage was calculated according to the dry matter intake recorded the previous day.
The amounts of TMR offered and refused were recorded daily for each lamb and were adjusted to ensure ad libitum consumption (refusal of about 10 % of DM intake). For each lamb, individual refusals of feed were weighed daily during the experimental period and stored at −20 °C until further analysis. Offered and refusal feed samples were analyzed for DM and other nutrients in order to evaluate daily nutrient intake. Lambs were weighed at the beginning of the experiment and subsequent weights were measured every 15 days before the morning feeding. Then, total gain and average daily gain (ADG) for lambs were calculated by subtraction of the initial from final BW and then dividing by duration of the study (51 days). Feed conversion ratio (FCR = DM intake/ADG) and feed conversion efficiency (FCE = ADG/DM intake) were also calculated.
At the end of the study, three lambs of similar BW and DM intake from each treatment were randomly selected and adapted to a pants and harness designed for total fecal collection to measure nutrient digestibilities. Lambs had 2 days of an adaptation period to pants and harness followed by 5 days of sample collection. Feed intake and refusals were recorded and sampled for further analysis. Daily fecal output was collected, weighed, and recorded. A sub-sample (10 % of total feces) was stored (−18 °C) for latter analysis.
TMR diet and refusal samples were dried at 55 °C in a forced-air oven to reach a constant weight, air equilibrated and ground to pass through 1 mm sieve. Samples of TMR diet offered and feed refusals were analyzed for dry matter (DM; 105 °C in forced-air oven for 24 h), organic matter (OM; weight loss upon ashing at 550 °C for 6 h) and nitrogen (N; Kjeldahl procedure) according to AOAC (1990). The crude protein (CP) content was calculated by multiplying N by 6.25. Neutral detergent fiber (NDF; with heat stable α-amylase and Na sulfite) and acid detergent fiber (ADF) content (Van Soest et al. 1991) were analyzed using the ANKOM F-57 filter bags in an ANKOM200 fiber analyzer (ANKOM Technolgy, Macedon, NY USA). Values for NDF and ADF were not corrected for ash contents. Hemicellulose content was calculated from the difference between NDF and ADF. Samples of the dried feces, offered and refused feed obtained from the digestibility study were analyzed for DM, OM, CP, NDF and ADF to calculate nutrient digestibilities.
Data were analyzed as a randomized complete design with three treatments (Control, TMR-E and Oral-E) with seven repetitions per treatment (three in case of digestibility traits) using the GLM procedure of the statistical package SAS version 9.2 (SAS Institute, Cary, NC, USA). The linear model included the fixed effect of treatment. Initial BW was included as a covariate for the BW data. Orthogonal contrasts were used to determine effect of enzyme addition (contrast 1, Control vs TMR-E and Oral-E) and application method (contrast 2, Oral-E vs TMR-E) on all dependent variables. Least squares means and standard errors for each treatment were obtained and used for multiple mean comparisons using the LSD test as implemented in the GLM procedure.
Effect of supplemental exogenous enzymes and application method on the productive performance and nutrient intake of growing Pelibuey male lambs fed a diet containing buffel grass
Contrastb (P values):
Initial BW (kg)
Final BW (kg)
Total gain (kg)
Nutrient intake, g/d
Feed conversion efficiencyd
Feed conversion ratiod
Effect of supplemental exogenous enzymes and application method on nutrient digestibility of growing Pelibuey male lambs fed a diet containing buffel grass
Contrastb (P values):
Research on effects of adding EE on nutritive value of the diet and animal performance are not consistent. In this study, the addition of the EE product did not improve intake of nutrients, but ADG of the lambs was improved possibly due to the improvement of nutrient digestibility. Previous research reported that application of EE preparations of different compositions improved DM intake and growth performance of lambs (Cruywagen and van Zyl 2008), goat kids (Titi 2003) and beef steers (Beauchemin et al. 1999; McAllister et al. 1999) and nutrient digestibility and milk production in dairy cattle (Yang et al. 1999; Gado et al. 2009). In contrast, other studies have reported that EE supplementation failed to improve DM intake and growth performance of lambs (McAllister et al. 2000; Mora-Jaimes et al. 2002; Rojo et al. 2005; Miller et al. 2008a; Awawdeh and Obeidat 2011) and beef steers (Krueger et al. 2008; Miller et al. 2008b).
This study found that the EE product improved the digestibility of DM, OM, NDF and ADF, but not the digestibility of CP of this diet for Pelibuey lambs. These results agree with most the published literature reporting increased total tract digestibilities of DM, OM or both, following treatment with a fibrolytic enzyme mixture (Rode et al. 1999; Yang et al. 1999; Beauchemin et al. 2000). Specifically, in a diet for lambs, Titi and Tabbaa (2004) reported that digestibility of nutrients was improved with the addition of cellulase enzyme, but the digestibility of CP was not affected.
It has been suggested that improved digestibility caused by EE treatment of feed might be related to improved microbial colonization (Yang et al. 1999; Nsereko et al. 2002) or both improved colonization and increased activity of the EE within the rumen (Beauchemin et al. 2000). This view is similar to previous hypotheses that exogenous enzymes increased fibrolytic activity due to increased numbers of ruminal microbes, and increased bacterial attachment and synergistic effects with hydrolysis of ruminal microorganisms (Colombatto et al. 2003).
This study was also designed to compare the efficacy of two methods of enzyme application. Some authors have suggested that pre-treatment of feed with enzymes could create a stable enzyme-feed complex (Kung et al. 2000), but others have indicated an alteration in the fiber structure, which would stimulate microbial colonization (Nsereko et al. 2000). In our study the FCE, FCR and ADF digestibility was affected more by application method. Several reports on animal response to feed enzymes mentioned that applying fibrolytic exogenous enzymes in a liquid form onto feeds prior to consumption could have a positive effect on animal performance (Rode et al. 1999; Yang et al. 2000). In contrast, direct infusion of enzyme into the rumen is far less effective or can even reduce digestibility of forage compared with application of liquid enzyme to substrate (Lewis et al. 1996).
In conclusion, supplemental EE can modify body weight gain of Pelibuey lambs and nutrient digestibilities without affecting nutrient intake, but the results of feed conversion efficiency and improved ADF digestibility depend on the application method used.
This manuscript is part of Ph.D. Dissertation of DLA where JHM, AZMS and JFVA were members of the advisory committee. DLA, JHM, JCMG and SR collected field data, DLA, RRR, JFVA and NLV performed statistical analyses, DLA, FSD, JFVA and NLV prepared the manuscript. All authors read and commented on drafts prepared and finally approved the manuscript. All authors read and approved the final manuscript.
This work was undertaken with funds from the Universidad Autónoma del Estado de México (project UAEM 3201/2012CHT). Daniel Lopez Aguirre acknowledges the scholarship granted by the National Council for Science and Technology (Consejo Nacional de Ciencia y Tecnología-CONACYT, Mexico).
The authors declare that they have no competing interests.
The experimental protocol utilized with the animals in this study completely adhered to ethical issues, since international recognized guidelines were followed.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- AOAC (1990) Official methods of analysis, 16th. Association of Official Analytical chemist, GaithersburgGoogle Scholar
- Awawdeh MS, Obeidat BS (2011) Effect of supplemental exogenous enzymes on performance of finishing Awassi lambs fed olive cake-containing diets. Livest Sci 138:20–24View ArticleGoogle Scholar
- Beauchemin KA, Yang WZ, Rode LM (1999a) Effects of grain source and enzyme additive or grain source on site and extent of nutrient digestion in dairy cows. J Dairy Sci 82:378–390View ArticleGoogle Scholar
- Beauchemin KA, Rode LM, Maekawa M, Morgavi DP, Kampen R (2000) Evaluation of a nonstarch polysaccharidase feed enzyme in dairy cow diets. J Dairy Sci 83:543–553View ArticleGoogle Scholar
- Colombatto D, Mould FL, Bhat MK, Morgavi DP, Beauchemin KA, Owen E (2003) Influence of fibrolytic enzymes on the hydrolysis and fermentation of pure cellulose and xylan by mixed ruminal microorganisms in vitro. J Anim Sci 81:1040–1050View ArticleGoogle Scholar
- Cruywagen CW, van Zyl WH (2008) Effects of a fungal enzyme cocktail treatment of high and low forage diets on lamb growth. Anim Feed Sci Technol 145:151–158View ArticleGoogle Scholar
- Gado HM, Salem AZM, Robinson PH, Hassan M (2009) Influence of exogenous enzymes on nutrient digestibility, extent of ruminal fermentation as well as milk production and composition in dairy cows. Anim Feed Sci Technol 154:36–46View ArticleGoogle Scholar
- Krueger NA, Adesogan AT, Staples CR, Krueger WK, Kim SC, Littell RC, Sollenberger LE (2008) Effect of method of applying fibrolytic enzymes or ammonia to bermudagrass hay on feed intake, digestion, and growth of beef steers. J Anim Sci 86:882–889View ArticleGoogle Scholar
- Kung L Jr, Treacher RJ, Nauman GA, Smagala AM, Endres KM, Cohen MA (2000) The effect of treating forages with fibrolytic enzymes on its nutritive value and lactation performance of dairy cows. J Dairy Sci 83:115–122View ArticleGoogle Scholar
- Lewis GE, Hunt CW, Sanchez WK, Treacher R, Pritchard GT, Feng P (1996) Effect of direct-fed fibrolytic enzymes on the digestive characteristics of a forage-based diet fed to beef steers. J Anim Sci 74:3020–3028View ArticleGoogle Scholar
- Martin C, Michalet-Doreau B (1995) Variations in mass and enzyme activity of rumen microorganisms: effect of barley and buffer supplements. J Sci Food Agric 67:407–413View ArticleGoogle Scholar
- McAllister TA, Oosting SJ, Popp JD, Mir Z, Yanke LJ, Hristov AN, Treacher RJ, Cheng K-J (1999) Effect of exogenous enzymes on digestibility of barley silage and growth performance of feedlot cattle. Can J Ani Sci 79:353–360View ArticleGoogle Scholar
- McAllister TA, Stanford K, Bae HD, Treacher RJ, Hristov AN, Baah J, Shelford JA, Cheng K-J (2000) Effect of a surfactant and exogenous enzymes on digestibility of feed and on growth performance and carcass traits of lambs. Can J Anim Sci 80:35–44View ArticleGoogle Scholar
- Miller DR, Elliott R, Norton BW (2008a) Effects of an exogenous enzyme, Roxazyme® G2 Liquid, on digestion and utilization of barley and sorghum grain-based diets by ewe lambs. Anim Feed Sci Technol 140:90–109View ArticleGoogle Scholar
- Miller DR, Elliott R, Norton BW (2008b) Effects of an exogenous enzyme, Roxazyme® G2, on intake, digestion and utilisation of sorghum and barley grain-based diets by beef steers. Anim Feed Sci Technol 145:159–181View ArticleGoogle Scholar
- Mora-Jaimes G, Bárcena-Gama R, Mendoza-Martínez GD, González-Muñoz SS, Herrera-Haro J (2002) Performance and ruminal fermentation in lambs fed sorghum grain treated with amylases. Agrociencia 36:31–38Google Scholar
- Morgavi DP, Beauchemin KA, Nsereko VL, Rode LM, McAllister TA, Iwaasa AD, Wang Y, Yang WZ (2001) Resistance of feed enzymes to proteolytic inactivation by rumen microorganisms and gastrointestinal proteases. J Anim Sci 79:1621–1630View ArticleGoogle Scholar
- Mould FL, Ørskov ER (1983) Manipulation of rumen fluid pH and its influence on cellulolysis in sacco, dry matter degradation and the rumen microflora of sheep offered either hay or concentrate. Anim Feed Sci Technol 10:1–14View ArticleGoogle Scholar
- National Research Council (2007) Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids. National Academy Press, WashingtonGoogle Scholar
- Noziere P, Besle JM, Martin C, Michalet-Doreau B (1996) Effect of barley supplement on microbial fibrolytic enzyme activities and cell wall degradation rate in the rumen. J Sci Food Agric 72:235–242View ArticleGoogle Scholar
- Nsereko VL, Morgavi DP, Rode LM, Beauchemin KA, McAllister TA (2000) Effects of fungal enzyme preparations on hydrolysis and subsequent degradation of alfalfa hay fiber by mixed rumen microorganisms in vitro. Anim Feed Sci Technol 88:153–170View ArticleGoogle Scholar
- Nsereko VL, Beauchemin KA, Morgavi DP, Rode LM, Furtado AF, McAllister TA, Iwaasa AD, Yang WZ, Wang Y (2002) Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Can J Microbiol 48:14–20View ArticleGoogle Scholar
- Rode LM, Yang WZ, Beauchemin KA (1999) Fibrolytic enzyme supplements for dairy cows in early lactation. J Dairy Sci 82:2121–2126View ArticleGoogle Scholar
- Rojo R, Mendoza GD, González SS, Landois L, Bárcena R, Crosby MM (2005) Effects of exogenous amylases from Bacillus licheniformis and Aspergillus niger on ruminal starch digestion and lamb performance. Anim Feed Sci Technol 123–124:655–665View ArticleGoogle Scholar
- Titi HH (2003) Replacing soybean meal with sunflower meal with or without fibrolytic enzymes in fattening diets of goat kids. Small Rumin Res 48:45–50View ArticleGoogle Scholar
- Titi HH, Tabbaa MJ (2004) Efficacy of exogenous cellulase on digestibility in lambs and growth of dairy calves. Livest Prod Sci 87:207–214View ArticleGoogle Scholar
- Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fibre, neutral detergent fibre, and non-starch carbohydrates in relation to animal nutrition. J Dairy Sci 74:3583–3597View ArticleGoogle Scholar
- Wallace RJ, Wallace SJA, McKain N, Nsereko VL, Hartnell GF (2001) Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro. J Anim Sci 79:1905–1916View ArticleGoogle Scholar
- Yang WZ, Beauchemin KA, Rode LM (1999) Effect of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows. J Dairy Sci 82:391–403View ArticleGoogle Scholar
- Yang WZ, Beauchemin KA, Rode LM (2000) A comparison of methods of adding fibrolytic enzymes to lactating cow diets. J Dairy Sci 83:2512–2520View ArticleGoogle Scholar