Fig. 4
From: The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus
Indirect organogenesis from tuber-induced callus. a–c Developing yellowish green globular callus after 4 weeks. Callus formed on the edges of excised surface and proliferate on the entire surface of tubers; scale bars 0.5 cm. Note the purple colouration of callus in c. d Multiple shoot and root development from a single callus piece. Long shoots are trimmed to ease handling; scale bar 2 cm. e Multiple shoot apical meristems developing on callus; scale bar 0.8 cm. f An individual immature tuber with two shoots growing from the callus tissue; scale bar 0.6 cm. g–h Root development from callus tissue; scale bars 0.6 and 0.8 cm respectively