Table 4 Partial sequence analysis and enzyme characterization of thermophilic, cold-active and NaCl-tolerant endoglucanase from different strains

Optimum temperature

50–85 °C

60

80

55

Relative activity (%) at 0 °C

NR

NR

NR

34.5

Relative activity (%) at 10 °C

NR

NR

NR

41.6

Relative activity (%) at 20 °C

NR

NR

NR

78.6

Optimum pH

5.0

6.5

3.6–4.5

5.5

pH stability

4.6–5.6 (80 %)

NR

NR

3.0–10.0 (>88 %)

NaCl-tolerant

4 M (70 %)

NR

NR

30 % (ca. 5 M) (83 %)

P (Pro)^a

5.45

5.65

6.41

5.43

H (His)^a

2.88

1.79

2.03

0.54

Hydrophobic AA^a

39.43

42.26

44.23

39.13

Total ASA

13,645.64

12,450.21

21,411.93

13,701.91

Exposed nonpolar ASA

8224

7616.52

13,451.81

7541.92

Total volume (packing)

42,258

41,660.11

61,825.52

46,735.51

Nonpolar relative ASA

0.61

0.62

0.63

0.55

Accession no.

ACI19154

GQ849224

LN626709

KR150023

Organism

Dictyoglomus thermophilum

Yak rumen metagenome

Alicyclobacillus vulcanalis

This study

Reference

Shi et al. (2013)

Bao et al. (2011)

Boyce and Walsh (2015)

This study