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Table 3 Enzyme activity of wild and mutant type recombinant PgluE8 E55A, PgluE8 D116A, PgluE8 C23G, PgluE8 C364G and PgluE8 C23G-C364G

From: NaCl-, protease-tolerant and cold-active endoglucanase from Paenibacillus sp. YD236 isolated from the feces of Bos frontalis

Enzyme

Activity (U/mg)

Relative activity (%)a

Wild type PgluE8

5.86 ± 0.23

100.00

PgluE8 E55A

0.52 ± 0.12

8.87

PgluE8 D116A

0.71 ± 0.09

12.12

PgluE8 C23G

8.60 ± 0.30

148.08

PgluE8 C364

8.24 ± 0.13

140.46

PgluE8 C23G-C364G

9.10 ± 0.42

155.28

  1. aValues represent the mean ± SD (n = 3) relative to the untreated control samples