- Case study
- Open Access
Nanofat grafting under a split-thickness skin graft for problematic wound management
© Kemaloğlu. 2016
- Received: 19 November 2015
- Accepted: 12 February 2016
- Published: 20 February 2016
Obesity and certain medical disorders make the reconstruction of skin defects challenging. Different kind of procedure can be used for these defect, besides, skin grafting is one of the most common and simplest procedure. Fat grafting and stem cells which are located in the adipose tissue have been commonly used in plastic surgery for regeneration and rejuvenation purposes. To decrease graft failure rate we performed nanofat grafting under an autologous split-thickness skin graft in our patient who had a problematic wound.
The case of a 35-year-old female patient with a traumatic skin defect on her left anterior crural region is described herein. After subsequent flap reconstruction, the result was disappointing and the defect size was widened. The defect was treated with combined grafting (nanofat grafting under an autologous split-thickness skin graft). At the 6 months follow-up assessment after combined grafting, the integrity of the skin graft was good with excellent pliability.
Combined grafting for problematic wounds seems to be a useful technique for cases requiring reconstruction. The potential existence of stem cells may be responsible for the successful result in our patient.
- Split-thickness skin graft
- Fat graft
- Stem cell
- Extremity reconstruction
Full-thickness skin defects frequently occur after trauma, vascular problems or tumor excision. Split-thickness skin grafts can be used to reconstruct these defects by applying them over the healthy recipient wound bed. Although split-thickness skin grafts can be easily used in lower limb reconstruction, they have higher failure and complication rates than those applied in other areas of the body since it is hard to keep the graft immobile. On the other hand, obese individuals are also at increased risk of wound complications including wound infection, dehiscence, hematoma, and seroma formation (Myers et al. 2007). Graft failure and chronic wounds in these patients are challenging problems and may also be expensive and time consuming to treat.
After the first reports on autologous fat grafting were published in the early twentieth century, it became popular in the plastic surgery armamentarium (Coleman 1995, 2001). Recent studies have demonstrated that the stromal-vascular cell fraction of adipose tissue represents a rich reservoir of regenerative precursor cells with proangiogenic capabilities (Zuk et al. 2001).
The term “nanofat grafting” was first used by Tonnard et al. and it can be used easily for skin rejuvenation purposes due to its small size and the fact that it contains stem cells (Tonnard et al. 2013).
The aim of this case report is to present an alternative method for managing chronic wounds of the lower limb by using nanofat grafting under an autologous split-thickness skin graft.
Lower extremity reconstruction can be complicated for both patients and surgeons. Unlike the head and neck, the lower limb is essential for mobilization and, as a result, movement can cause shear forces on the repair. Increased hydrostatic forces in the lower limb during mobilization may lead to hematoma or seroma formation. If there is no exposed bone, tendon or vital tissues exist, various techniques can be used like secondary healing, skin grafting or flap surgery. Although secondary healing is the simplest method, it can only be used for small defects. In defects which have a healthy wound bed, skin grafting is the most convenient technique for reconstruction.
A higher BMI has repeatedly been correlated with increased incidence of surgical complications, including atelectasis, thrombophlebitis, mortality, wound infection, flap necrosis and wound separation (Pitanguy et al. 2000; Matory et al. 1994; Selber et al. 2006). Thus, lower extremity reconstruction in obese patients can be challenging due to the possibility of potential wound healing problems. Although the use of a vacuum assisted closure device (VAC) on split-thickness skin grafts is associated with improved graft survival (Scherer et al. 2002), there is no evidence about the benefits of combined VAC therapy with skin graft in obese patients.
After the first adipose-derived stem cells were discovered in 2001, they have been used for various purposes, including the regeneration of missing tissues and rejuvenation (Rigotti et al. 2009; Rehman et al. 2004). Adipose-derived stem cells have an extensive proliferative capacity and the ability to differentiate into mesoderm, ectoderm, and endoderm lineages (Brzoska et al. 2007). To isolate stem cells, the stromal vascular fraction has to be separated from the adipocytes. However, isolating the stromal vascular fraction from the adipocytes before injection in routine clinical cases would be time consuming, complicated, and expensive. Therefore obtaining nanofat grafting is the simplest method to achieve adipose-derived stem cells and performing the injection with small cannulas in very small amounts is another advantage of this procedure. Although the regeneration properties of adipose-derived stem cells in clinical usage have been reported for radiodermatitis, atrophic scars, chronic ulcerations and antiageing therapy, this case is the first in the literature to report nanofat graft use for the management of a wound healing problem combined with skin grafting (Rigotti et al. 2007; Sarfati et al. 2011).
Due to the amount of dermis, full-thickness skin grafts provide better scar quality and less contraction. However, large defects can not be reconstructed by full-thickness skin graft because of limited donor area. Replacement of all the missing parts of a wound is essential to achieve the best scar and functional results. Therefore, the advantages of using a split-thickness skin graft were combined with nanofat grafting to obtain subdermal fat tissue and the stimulation of collagen by potential stem cells which are located in a nanofat graft (Tonnard et al. 2013).
To the best of our knowledge, the present study is the first to investigate the use of autologous split-thickness skin grafting with nanofat grafting. Because of previous skin graft failure history, a combined grafting technique was tried in our patient to provide better outcomes without increasing the complications. Stem cells can be differentiated to other cells in 2 weeks (Zuk et al. 2001). The probable mechanism of increased graft take, such as was observed in our obese patient, may have resulted from the stimulation of collagen and endothelial cells by stem cells. Endothelial cell stimulation promotes angiogenesis and these properties are very important not in the acute but in the vascular ingrowth phases of graft healing (Rigotti et al. 2007).
Regardless of the fact that adipose-derived stem cells have a function in combined grafting, molecular and immunohistochemical investigations should be conducted in future clinical studies. They may also be suitable for future use in combined grafting for difficult wounds, such as wound beds with poor granulation or small exposed tendons without paratenon.
Wound healing problems are common in obese patients. Split -thickness skin grafting is the simplest technique for the treatment of wounds which have good granulation tissue. Nowadays, adipose- derived stem cells are widely used in plastic surgery practice. Combining a skin graft with nanofat grafting may be an alternative for difficult cases. Further investigation which provides histopathologic and immunological evidence is necessary to support our findings.
The author declares that there is no competing interests.
There is no financial support related to this manuscript.
Informed consent was obtained from the patient regarding publication of the case report.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Brzoska M, Geiger H, Gauer S, Baer P (2007) Epithelial differentiation of human adipose tissue-derived adult stem cells. Biochem Biophys Res Commun 330:142–150View ArticleGoogle Scholar
- Coleman SR (1995) Long-term survival of fat transplants: controlled demonstrations. Aesthet Plast Surg 19:421–425View ArticleGoogle Scholar
- Coleman SR (2001) Structural fat grafts: the ideal filler? Clin Plast Surg 28:111–119Google Scholar
- Matory WE Jr, O’Sullivan J, Fudem G, Dunn R (1994) Abdominal surgery in patients with severe morbid obesity. Plast Reconstr Surg 94:976–987View ArticleGoogle Scholar
- Myers WT, Leong M, Phillips LG (2007) Optimizing the patient for surgical treatment of the wound. Clin Plast Surg 34:607–620View ArticleGoogle Scholar
- Pitanguy I, Gontijo de Amorim NF, Radwanski HN (2000) Contour surgery in the patient with great weight loss. Aesthet Plast Surg 24:406–411View ArticleGoogle Scholar
- Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE et al (2004) Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation 109:1292–1298View ArticleGoogle Scholar
- Rigotti G, Marchi A, Galie M et al (2007) Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose derived adult stem cells. Plast Reconstr Surg 119:1409–1424View ArticleGoogle Scholar
- Rigotti G, Marchi A, Sbarbati A (2009) Adipose-derived mesenchymal stem cells: past, present and future. Aesthet Plast Surg 33:271–273View ArticleGoogle Scholar
- Sarfati I, Ihrai T, Kaufman G, Nos C, Clough KB (2011) Adipose-tissue grafting to the post-mastectomy irradiated chest wall: preparing the ground for implant reconstruction. J Plast Reconstr Aesthet Surg 64:1161–1166View ArticleGoogle Scholar
- Scherer LA, Shiver S, Chang M, Meredith JW, Owings JT (2002) The vacuum assisted closure device: a method of securing skin grafts and improving graft survival. Arch Surg 137:930–933View ArticleGoogle Scholar
- Selber JC, Kurichi JE, Vega SJ, Sonnad SS, Serletti JM (2006) Risk factors and complications in free TRAM flap breast reconstruction. Ann Plast Surg 56:492–497View ArticleGoogle Scholar
- Tonnard P, Verpaele A, Peeters G, Hamdi M, Cornelissen M, Declercq H (2013) Nanofat grafting: basic research and clinical applications. Plast Reconstr Surg 132:1017–1026View ArticleGoogle Scholar
- Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ et al (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228View ArticleGoogle Scholar