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Fig. 7 | SpringerPlus

Fig. 7

From: Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as possible markers of amino acid and fatty acid catabolic pathways by LC–ESI–MS/MS

Fig. 7

Influence of standing saliva samples at RT on stability of 3HB (a), 3HIB (b), 3HMB (c), and 2HB (d). Saliva samples collected from healthy volunteers (n = 4) were aliquoted into seven microcentrifuge tubes and standing at RT for various times (0, 1, 2, 4, 6, 10, and 24 h). Thereafter, the saliva samples were stored at −20 °C until analysis. RT room temperature. See legends of Figs. 1, 2, and 3 for other abbreviations. Data are shown as mean ± SEM. Statistical analysis by one-way ANOVA multiple comparison and a post hoc Dunnett’s multiple comparison test. ANOVA P values are a P = 0.9949, b P = 0.9968, c P = 0.7058, and d P < 0.0001. *P < 0.05, ‡ P < 0.0001 vs. 0-time control

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