Table 4 Characteristics of the 26 long-core repeat primer pairs selected for fingerprinting of the five Prunus species
SSR ID | Pseudochrom. | TA | (SSR motif)n | Primer Forward | Primer Reverse | ||
|---|---|---|---|---|---|---|---|
Sequence | Peach v1.0 start | Sequence | Peach v1.0 start | ||||
RPPG1-017 | 1 | 56 | (AGCTT)5 | GCTCATCAAAACTCTCAACCA | 2,785,626 | CCCTTTCTTCAATCCCATC | 2,785,848 |
RPPG1-026 | 1 | 51 | (GAT)7 | CTTCTGGCACTCTTCCATTT | 4,980,754 | GTTCCCAAGTTTTCCTCTCA | 4,980,991 |
RPPG1-032 | 1 | 53 | (CTT)7 | ATGGCAGAGAGCACAACAA | 22,022,341 | TTGAGAGGTAACAGCGAGAA | 22,022,564 |
RPPG1-037 | 1 | 53 | (AGC)7 | GTCTCTGATCCAAGCCAACT | 42,186,590 | ACGCTGCCATTGTTTCTATT | 42,186,831 |
RPPG1-041 | 1 | 56 | (ATT)7 | TGTTGTAATGGATGGTGTCTTC | 44,374,756 | CTTGGTCTTGGTTTCATTCA | 44,374,983 |
RPPG2-011 | 2 | 53 | (ATTT)5 | TTTACAGGTGCCTCAACAAA | 3,728,089 | GTACAGCCGATGGAGAGAAA | 3,728,287 |
RPPG2-022 | 2 | 53 | (CTGT)6 | CTGCTGCGTCTGATGATG | 26,576,155 | ACAGGACAGGACCACTTTCT | 26,576,364 |
RPPG3-026 | 3 | 53 | (CTGT)6 | AGAACGCTATTCCCCTGTAA | 3,151,168 | TCATCCTCTCCAAATGTCAA | 3,151,412 |
RPPG4-059 | 4 | 56 | (ACTGG)6 | GACGGCTGTTTATTTGCATT | 138,756 | TGCATTTGTGATCTCGTTTC | 138,937 |
RPPG4-067 | 4 | 58 | (GGTTT)4 | AGAAGGGAGGGTGAGAGAAG | 3,564,871 | CACGAAGGAAGAAACGAAGT | 3,565,136 |
RPPG4-077 | 4 | 57 | (AATT)5 | CCTCGTCTTCAGTCTTTTCTG | 18,611,275 | CTGTCCCTTCTGTGTTCCTAA | 18,611,433 |
RPPG4-084 | 4 | 58 | (ATTT)5 | TCCTCAAAAGTTACCCCAAG | 26,137,915 | CTTGCTGTGGAAGAAGAACC | 26,138,190 |
RPPG4-091 | 4 | 49 | (CTTTT)6 | GGAGGGTAGAGAACAGAGCA | 27,055,301 | CGGAAGATGTGATTGTGAGA | 27,055,542 |
RPPG5-018 | 5 | 56 | (ATT)8 | GCATGAAATTGACCCATACA | 5,331,336 | TAATTGCTTTGGGGAGGAC | 5,331,523 |
RPPG5-022 | 5 | 58 | (ATC)11 | CTTGTGAACTGGCATCTGTC | 8,805,836 | AGTTGTATGGGCATGTTGTG | 8,806,134 |
RPPG5-023 | 5 | 56 | (ATT)7 | TTGTTTGCACTAGGCTTTGA | 16,625,324 | TTCTTCTTGCATGTCCTTGA | 16,625,517 |
RPPG5-025 | 5 | 53 | (CCCTT)5 | GTGTCTCCTCCTCAAAGCAA | 16,792,568 | TACGGCAACCAAGAACATC | 16,792,866 |
RPPG5-030 | 5 | 53 | (AATT)5 | AAGGCAAGGAATTGGGTAGT | 18,027,410 | TGGTTTGTCGTAAGAGTCCA | 18,027,575 |
RPPG6-009 | 6 | 53 | (GTTTT)4 | GGGCTTGGCTGATAAAATAA | 1,068,427 | TGGTAAAATAGAAGAGCGAGAAG | 1,068,608 |
RPPG6-032 | 6 | 53 | (ATCGC)5 | TCCTATGGCAAAAACAAAATC | 26,949,411 | TGAAGAGATGGAGTGGAAGAG | 26,949,563 |
RPPG6-033 | 6 | 56 | (CTGT)6 | CATTATCAAACCACGACCAA | 27,071,911 | AAAGCTCAACAGCGACTTCT | 27,072,026 |
RPPG7-015 | 7 | 58 | (ATTT)6 | TCTTGGTGGTGGTGAAGTAA | 2,533,650 | GAGAGATGGAGGAGGCTGA | 2,533,925 |
RPPG7-026 | 7 | 53 | (ACATT)4 | TTTGGTGAGTGGGCTCTATT | 18,786,038 | CTATCGTTCGCTGGTCTTCT | 18,786,203 |
RPPG7-032 | 7 | 53 | (AGG)7 | AAGGGAGGAGGATTGTGAA | 22,275,889 | TGGTAGACGGGTAGATGTTG | 22,276,079 |
RPPG8-007 | 8 | 53 | (GGT)7 | ACCACCACCTCTTCCAATC | 86,262 | ACCTCAAAGTGTCCCAGAAA | 86,469 |
RPPG8-028 | 8 | 58 | (AACCC)6 | AAGGAGCCGACATCAGAAC | 20,410,671 | TGACCAGAAGCCAAATACATC | 20,410,876 |