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Table 3 Characteristics of the P. pubescens SSR markers derived from FL-cDNAs

From: Identification, cross-taxon transferability and application of full-length cDNA SSR markers in Phyllostachys pubescens

No.

Marker

Accession no.

Primer sequence (5′→ 3′)

Motif

Tm (°C)

PCR fragment (bp)

 PIC

Putative function

Name

cDNA

SSR

1

PBM031

FP094740

GU644371

CGCCGAGTTCCCTATTATTATTT

(AG)6-(AG)7

56

191

0

MYB-like transcription factor

    

AGCACAGCCTCCGTGATTG

     

2

PBM032

FP098085

GU644372

TTTCCCAAATAAAACCTCACC

(CCG)7-(CCT)6

56

143

0

PHD finger protein

    

GTCCATTTAGGGTTCCACTGA

     

3

PBM033

FP099510

GU644373

CTGACTGTGCGTGCGTCTC

(CG)8(AG)14

56

155

0

Small GTP-binding protein

    

CTTGGTCTCGCTCATCTCCTC

     

4

PBM034

FP098748

GU644374

TCGGCTCGGCGTGATGGAT

(GAG)5(GCG)5

62

169

0

GTP binding protein

    

ATCGGCATCCGCGACTGCC

     

5

PBM035

FP100911

GU644375

ACCGTGATGACTACCGCCGCGACC

(GTG)7-(GTG)7

62

165

0.368

U2 snRNP auxiliary factor

    

TGCTGCCTCCACCCCTCCGTCC

     

6

PBM036

FP096684

GU644376

CACATGGACCGCCTCATCC

(TA)8

47

169

0.259

Polypeptide-associated complex alpha subunit-like protein

    

GCAACAAAACGAGAACCAGAC

     

7

PBM037

FP101192

GU644377

TGCAAGCCTGCTATACGTTT

(TA)7-(TA)6

47

130

0

Thaumatin family protein

    

GAAGTGGGAGTACATACTTCCCA

     

8

PBM038

FP101125

GU644378

GGTCGGCTCATTTTGTAGTGT

(TC)9(TA)22

48

210

0.365

GCIP-interacting family protein-like

    

CAACCTTCAGGCAATAGATTACAT

     

9

PBM039

FP091409

GU644379

CATCCTCAGTTTCTCACCG

(TC)12-(CTT)6

53

171

0.355

Unknown protein

    

CAGCTTCACCAACTTGTGG

     

10

PBM040

FP096343

GU644380

GAATCATCTGGGAAGAAGAAGGA

(TC)7-(TC)7

51

178

0

Bicolor hypothetical protein

    

TGCATTGCATTTGGCTTAGTAGT

     

11

PBM041

FP095242

GU644381

TGGTGTTGCCTGTGACCTTAC

(TG)8(AG)10

53

167

0

typeA response regulator 1

    

CCCACCTCCACCTCTACTACG

     

12

PBM042

FP093940

GU644382

TCCTTTACGGCTTTACCCC

(GA)7-(AG)6

53

156

0.365

SAM and SH3 domain-containing protein 1

    

GCCCCAGCTTAGTACACCAC

     

13

PBM043

FP099127

GU644383

CTCACCGCCCCACCTCGCA

(AG)13

60

128

0

IAA15 - auxin-responsive Aux/IAA family member

    

CGGCTGCTGATGCGGAGGA

     

14

PBM044

FP095585

GU644384

AAGGCCCACGTTGCCAGAC

(AG)20

55

173

0.371

Bicolor hypothetical protein

    

GTTCCCGTTGATGCCCCAC

     

15

PBM045

FP098751

GU644385

TGAGCGAGGTAGTTTCATTTTAGTTA

(CA)20

53

132

0.322

DRE binding factor

    

CCTACGACGAGTAGATTGCGAGT

     

16

PBM046

FP094276

GU644386

CTCAGAGCAGACACTGCTTATTCC

(CT)5-(CT)6

50

102

0.395

Unknown protein

    

GCGTCTTCATTGCAGCCATCT

     

17

PBM047

FP099829

GU644387

ACCACGTTGCAGGATTCACT

(CT)13

53

119

0

Bicolor hypothetical protein

    

CGATGAGCAGCACAACAGC

     

18

PBM048

FP092637

GU644388

GCAAAAGAGCGCACTTGAC

(CT)27

53

163

0

Serine carboxypeptidase 1 precursor

    

GGAGGACACTAGAGTTGGCATT

     

19

PBM049

FP099913

GU644389

ACAGCAGATAGTCCCAAAAT

(GA)14

50

117

0.305

Unknown protein

    

GACAGCAGGATGAAGAGCA

     

20

PBM050

FP093015

GU644390

AGTATAGTATGTTCGTTTAAGTGG

(CA)11

45

137

0

Oxidoreductase

    

TGTAATGTTTAAGGTTCCGT

     

21

PBM051

FP092618

GU644391

AGACATTGTCAACTGTAAGTTGGTAGAG

(TC)23

50

111

0

VQ motif family protein

  

(FP099842)

 

TTTACAAGCAATACACCCAGAAATAG

     

22

PBM052

FP095787

GU644392

AGCGGGCAGGCTATGTATT

(TCT)11

51

140

0.359

ELF4-like protein

    

TTGCTTCTCCCCTAATGACA

     

23

PBM053

FP094717

GU644393

CCCCATAATCTGCTCCCTTCT

(TTC)10

51

102

0

KN1-type homeobox transcription factor

    

GGTTCTTGGCGTATGGTATGTTC

     

24

PBM054

FP100158

GU644394

ATCGGGAGGGATGCGGCAGC

(GGCGGA)6

62

121

0.305

Unknown protein

    

GCGGACCAAGCGGAACACC

     

25

PBM055

FP100601

GU644395

CATGGATGTTGTTGAGTTGAGGC

(TC)7

53

199

0

Nonspecific lipid-transfer protein 2 precursor

    

GCACAAAGACTAGTACTCGAGGTGG

     

26

PBM056

FP100601

GU644396

CATGGATGTTGTTGAGTTGAGGC

(CTCCAT)6

53

177

0

Nonspecific lipid-transfer protein 2 precursor

    

GCACAAAGACTAGTACTCGAGGTGG

     

27

PBM057

FP097951

GU644397

CGCCCACCCCTCCTTCGTCT

(ACACAG)5

59

111

0

Cp protein

    

TCCTTGGCACGGCCACTCA

     

28

PBM058

FP097794

GU644398

GGCCGAGATCCTCCTTTCT

(GGCGGT)5

59

171

0

Unknown protein

    

CCATCCCCGCCTTCACCAC

     

29

PBM059

FP094127

GU644399

ATTAGTCACGCACCGAGAAGGAA

(AGATG)6

55

172

0

Transcription elongation factor-related protein

    

AGACGCAAGAACTCGACAGGGA

     

30

PBM060

FP101691

GU644400

CACGCCAGCTCCAGATGCCACCAT

(CACCC)5

59

119

0

Sucrose transporter

    

TGCCCTTCCACCTCCTCTGACCTCC

     

31

PBM061

FP095238

GU644401

CCCTATCCCATCCTCCTCCC

(CCTCT)5

55

119

0

Smr domain containing protein

    

GGTTGCTCACTTTCCTGCTCC

     

32

PBM062

FP096136

GU644402

TGCTGGTTGGGTTCATCACGA

(TTCT)7

53

156

0

Bicolor hypothetical protein

    

GAGGGTTACAACAGGGGCAAAGA

     

33

PBM063

FP098746

GU644403

CAACGCAACGCCATTCCAAACA

(TCCA)5

59

138

0

U-box domain containing protein

    

CACCTCCAGGCCCTGGTACTCCA

     

34

PBM064

FP099572

GU644404

CATTTCTCATTGCCGCTGTAAC

(GAGT)5

53

139

0

Unknown protein

    

TCCTTTGCCCTCCTCTTCCT

     

35

PBM065

FP096965

GU644405

GTCAGTCAGGCGGCACGAG

(CG)5-(CGG)9

60

183

0

Bicolor hypothetical protein

    

CGCGTAGGACGAGATCACCTC

     

36

PBM066

FP095562

GU644406

CTCTTCACCGAAACCGAAAG

(CGG)9

57

137

0.477

Spliceosomal protein

    

CGTTGAGGTTCCTGAGGTAGAC

     

37

PBM067

FP098504

GU644407

GGTGCGGGTGCAGTTTATT

(CTT)8

51

185

0

RNA-binding protein

    

AGCATCATCCGCCAGAATA

     

38

PBM068

FP093884

GU644408

AACCGTGCACTACTTGCTCT

(TCT)8

51

155

0

pollenless3 mRNA

    

ACCTTGTGGACGACATGGA

     

39

PBM069

FP099427

GU644409

CCCTTTCCCTTCAACAACAA

(CCG)8

57

101

0.360

Alba superfamily protein

    

TACCGATCCATGGCTCCTT

     

40

PBM070

FP094239

GU644410

TCGTGCCTTTCGCCTCCTG

(TCT)7

55

117

0

Bicolor hypothetical protein

    

CTGTACGGCCCGAACTTGTA

     

41

PBM071

FP093953

GU644411

AGCGTCACCTCCGCCTTCT

(CGA)8

57

101

0

Unknown protein

    

TCCTTGGCCTCGTCTTGGT

     

42

PBM072

FP093285

GU644412

CCTCCCACTGTCACGGCACC

(CTC)9

59

116

0

Bicolor hypothetical protein

    

GGCTGTGGCGACAAGGCTG

     

43

PBM073

FP096973

GU644413

AGCAGCTCTACGGCAAGAAGAAG

(ATC)8

53

139

0

Bicolor hypothetical protein

    

TGCAGCCTTGAGGAATTGAGAA

     

44

PBM074

FP096816

GU644414

CCCACCGAAGTAATCACGC

(CTC)8

55

119

0

Transcription factor HBP-1a(c14)

    

CTCGCACAACAAAAGAAATCA

     

45

PBM075

FP096707

GU644415

AGTTTCCTTCTTTCCTTCCTTCCGTGGTG

(GAA)8

53

101

0.510

Unknown protein

    

CGGCATTTGCGATTTGTGC

     

46

PBM076

FP101632

GU644416

ATGCCTTCACCACACTTAC

(GCA)8

51

121

0

AP2/ERF domain protein

    

CATCGTGATGTCTCCAATC

     

47

PBM077

FP096443

GU644417

CCGCTTCCTCCCACCAAAT

(CCG)5

59

181

0

Bicolor hypothetical protein

    

CGCAGTACAGCAGCTCCCC

     

48

PBM078

FP095554

GU644418

CCCAAATCCAACCAGAACCA

(CGG)11

59

187

0

Anti-silencing protein

    

GGAGGAGGCATTCGTAGGAGA

     

49

PBM079

FP097911

GU644419

AAGGATGGTAACGTACATACA

(AT)5

44

159

0

Unknown protein

  

(FP092888)

 

CATGACAAATTTAAAGGTATCA

     

50

PBM080

FP093425

GU644420

CGAGGTTCTTGGGCTCAGTT

(AG)13

53

116

0.375

ATP binding protein

    

ACACGCCTCCAATAAAACAAAC

     

51

PBM081

FP097485

GU644421

TCTACTCCGTAGCCGCCTTC

(CT)16

56

135

0

Pyridoxamine 5-phosphate oxidase

    

AGAGCCTCCATTGGATGGG

     

52

PBM082

FP099753

GU644422

AATTTGTTGCCCTGCCTAGCT

(TC)5-(TC)16

53

148

0

Homeodomain leucine-zipper protein Hox8

    

GCAAGATGAGAAGAATTAAAGCTGC

     

53

PBM083

FP101428

GU644423

CCATTTGGCATTTGCTCCC

(GA)15

59

186

0

GTPase SAR1 (Sar1.1)

    

GCACCCCGTAGAACCAGTCC

     

54

PBM084

FP092513

GU644424

CTTCTCATGGGGTCAGCTACTC

(TC)17(AC)16

53

201

0.369

Brown planthopper-induced resistance protein 1 (Bi1)

    

ATCACTTCTGCGATCTTGGTC

     

55

PBM085

FP091409

GU644425

GGGGAGCCATCCTCAGTTT

(TC)12-(CTT)6

55

183

0.346

Putative precursor micro RNA R167h gene

    

GCTGGCAGCTTCACCAACT

     

56

PBM086

FP096167

GU644426

GTGGAAAATAAAGAAGCGC

(TC)9-(TC)9

51

139

0

Unknown protein

    

TTCCTGCTTTTGATCTTGC

     

57

PBM087

FP093957

GU644427

ACCCCAAGCATCCCCAAAA

(CCT)5-(CGC)9

59

166

0.373

Bicolor hypothetical protein

    

CCGCAGGGAAGTCGAAGGTC

     

58

PBM088

FP091571

GU644428

GTGTATTGGCTTTCCAGCTTTTCC

(AG)11

55

211

0

Knotted class 1 homeodomain protein

    

TCTCCGCACGCTACTGTCCC

     

59

PBM089

FP097920

GU644429

TCCCTTATCCACCAAACACGC

(CT)17

56

172

0.369

Bicolor hypothetical protein

    

GCTGGCAACGACGCACCTC

     

60

PBM090

FP097267

GU644430

AGAGTCGGATAAGGGTAGCG

(AG)12

53

106

0.195

Repair protein RAD23

    

CGATCTCGAAGTTCGTGCC

     

61

PBM091

FP100553

GU644431

ATAGAGGCATACAGCCGCAGAC

(AG)14

56

126

0.369

Macrophage migration inhibitory factor

    

TAGGCACGGCATCACGGAC

     

62

PBM092

FP099642

GU644432

GAACGCCGCATCCAGCCTCT

(TC)13

53

155

0

Basic/leucine zipper protein

    

GGTCGGGTCCTTGGACAAAC

     

63

PBM093

FP100738

GU644433

TCGCAGTAAACAGTCTCATCACATC

(CCT)8

59

150

0

Disulfide isomerase (PDIL2-2)

    

TCAGGGCCACCACCTCGTCT

     

64

PBM094

FP095169

GU644434

GATTGAGGAGCCCCAAACC

(CCG)8

57

257

0

DUF2372 superfamily protein

    

CACAACAACCGCAAGAGCC

     

65

PBM095

FP098630

GU644435

TTATTAGTCGAGTTTGGGTCTCC

(CCT)8

55

115

0.430

Unknown protein

    

GGTGAACGGCATGGCTGCT

     

66

PBM096

FP100124

GU644436

CACTCGGCTCGTCCTCGTCT

(CCTC)6

60

129

0

PLAC8 superfamily protein

    

AGGGTGGCTAAGGCTCGTCTC

     

67

PBM097

FP099849

GU644437

CTGCCACTCCATCCCTGCC

(CACGCG)5

59

101

0

Unknown protein

    

CTCGATGGCGACGGCTGTT

     

68

PBM098

FP097471

GU644438

CCCCGTCTTCTCGTCGTCT

(TCGCCG)5

56

169

0

BAH_BAHCC1 superfamily protein

    

GACTTTGTCGGAGCCCTTGA

     
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