Knowing the enemy: ant behavior and control in a pediatric hospital of Buenos Aires
© Josens et al.; licensee Springer. 2014
Received: 4 March 2014
Accepted: 24 April 2014
Published: 6 May 2014
Ant control is difficult in systems even where a variety of control strategies and compounds are allowed; in sensitive places such as hospitals, where there are often restrictions on the methods and toxicants to be applied, the challenge is even greater. Here we report the methods and results of how we faced this challenge of controlling ants in a pediatric hospital using baits. Our strategy was based on identifying the species present and analyzing their behavior. On the one hand, we evaluated outdoors in the green areas of the hospital, the relative abundance of ant genera, their food preferences and the behavioral dominances. On the other hand, control treatments were performed using separately two boron compounds added to sucrose solution which was not highly concentrated to avoid constrains due to the viscosity.
Most of the species in the food preference test accepted sugary food; only one species was recorded to visit it less than the protein foods. This result was consistent with the efficacy of control treatments by sugary baits within the rooms. For species that showed good acceptance of sugar solutions in the preference test outdoors, sugar bait control indoors was 100& effective. Conversely, for the only species that foraged significantly less on sugar food, the bait treatment was ineffective. This work reveals the importance of considering the behavior and feeding preferences of the species to be controlled by toxic baits.
KeywordsAnt Chemical control Baits Food preferences Nylanderia fulva
Many ant species are serious pests in urban environments. They may cause damage to structures, electronic devices and affect household residents; in addition, many species can sting and/or bite. Presently there is greater awareness of the risks involved in the indiscriminate release of toxicants on the environments we live and work, thus the demand for environmentally friendly control methods is growing. For ant control in urban settings, insecticides have been commonly used as perimeter barrier treatments (Mallis 1969; Ebeling 1978; Hedges 2010; Klotz et al. 2010). However, fast-acting barriers or sprays may result in ants trapped within the building in treatment (Klotz et al. 20082010) or in colony budding (Hedges 2010; Buczkowski et al. 2005; Oi 2008), even increasing the foraging activity indoors (Oi et al. 1996). The use of toxic baits is, undoubtedly, the least polluting way to control these insects and it is easy to be commercialized and applied both in homes and buildings. Exterior application of a delayed-action toxicant could result in successful control for indoor ant infestations (Oi et al. 1996).
Ants, as insects in general, have on their cuticle different microorganisms (Beatson 1972; Bueno and Campos-Farinha 1999; Santos et al. 2009), therefore they are frequently believed to be involved in food contamination and mechanical vectoring of disease agents (Beatson 1972; Fowler et al. 1993; Olaya-Masmela et al. 2005; Moreira et al. 2005). In addition, as there are minute species, they can enter places where other, larger insects, cannot (such as cockroaches, flies, mosquitoes, etc.). Moreover, ants travel long distances searching for food, circulating in these trails from several to hundreds of individuals. These characteristics give them the potential capacity to spread pathogens in sensitive places that should be kept aseptic (such as surgical environments, sites for storing sterile instruments or equipment, etc.) and move from hospital areas that may harbor pathogens to patients and between them (Beatson 1972).
Hospitals require careful attention; neonates, infants, elderly or immunosuppressed patients are more sensitive to insect stings and to different pathogens that insects could transport, but also to the pesticides used for their control. Pesticides in sprays, liquids or powders could represent a risk to these patients (Owens 2003). For these reasons, food baits containing a toxicant are recommended to control ants and cockroaches in these sensitive places (Owens 2003; Pampiglione and Velo 2010). This method has many advantages over others: on the one hand, it avoids toxic volatiles and reduces human toxicant exposure, and localization or access to the nest is not necessary as the ants distribute the bait amongst themselves. As social insects, ants live in a colony which presents division of labor among members. The reproductive caste rarely leaves the nest, but a proportion of the sterile workers do in order to forage on different resources. Hence, foragers themselves transport the bait towards the nest, where it is distributed among the remaining colony members. For these insects, the toxicant should have a delayed action, giving them enough time to allow foraging, transport to the nest, sharing therein and recruitment.
There are commercial toxic baits with different toxicants for most urban ants. They are commercialized in different countries as gels or similar vehicles contained in syringes. However, their success is variable (O’Brien and Hooper-Bui 2005; McDonald 2012). This could be due to many reasons that affect the behavior and decision making of the ant individuals during foraging. Foragers modulate their behavior according to the colony needs (Howard and Tschinkel 1980; Josens and Roces 2000; Falibene et al. 2009), the bait properties (Josens et al. 1998; Cassill and Tschinkel 1999; Medan and Josens 2005), the toxicant and its concentration (Hooper-Bui and Rust 2001; O’Brien and Hooper-Bui 2005; Sola et al. 2013), among many other factors. It is known that nectar ingesting ants do not always reach their full crop load and usually leave the food source with partial loads (Josens et al. 1998; Dornhaus et al. 2006). Highly viscous solutions are ingested at a very low rate (Josens et al. 1998; Medan and Josens 2005; O’Brien and Hooper-Bui 2005); ants drink longer and reach smaller loads than for more dilute or less viscous solutions (Josens et al. 1998). Besides, foragers can accept or reject certain baits, recruit other ants or not and intake faster or slower from a certain fluid depending on their motivation to feed (Howard and Tschinkel 1980; Josens and Roces 2000; Falibene et al. 2009; Falibene and Josens 2008; Mailleux et al. 2006). Moreover, behavioral responses to a certain toxic bait are not always the same among different species. For two species with similar feeding habits, like the carpenter ant Camponotus mus and the Argentine ant Linepithema humile (Newell and Barber 1913; Markin 1970; Hansen and Klotz 2005), boric acid can affect acceptance negatively for the former but not for the latter. On the other hand, borax may cause the inverse situation (Sola et al. 2013). Therefore, all these factors should be considered when developing baits for ant control.
Traditionally, the toxicological effects of pesticides were studied in cases of acute poisoning by accidental exposure to high doses of insecticide, which are well documented (Shannon et al. 2007). However, nowadays there is greater awareness of the risk of long term effects attributable to frequent exposure to doses of low concentrate pesticides (Kapoor et al. 2011; Herin et al. 2011). Also, new studies revealed the close relationship between the chronic health effects that become apparent at a given moment in life and the prolonged or repeated exposure to low doses of pesticides at earlier ages (Winans et al. 2011; Bailey et al. 2011). In this regard, pediatric hospitals should consider that their patients are particularly sensitive to pesticides, as they might have an impact on the development of a child and also contribute to the onset of diseases later (Winans et al. 2011). For example, several reports suggest an increase in the incidence of acute lymphoblastic leukemia in children by exposure to pesticides (Leiss and Savitz 1995; Infante-Rivard et al. 1999; Alexander et al. 2001; Thompson et al. 2001; Ma et al. 2002; Menegaux et al. 2006; Pombo-de-Oliveira and Koifman 2006; Rudant et al. 2007). Recently, a study conducted in Australia showed a correlation between the application of pest control treatments in homes and the posterior incidence of the mentioned pathology and also revealed a critical age period for children (2 to 3 years old) (Bailey et al. 2011). The authors argue that this window of greater sensitivity could be related to a greater exposure due to the habits and typical behavior of that age.
Taking all this into account, the pediatric hospital “Hospital de Niños Ricardo Gutiérrez” from Buenos Aires City has generally been trying to avoid the use of pesticide sprays or powders and has only been using commercial baits. Consequently, it has been facing some difficulties to control ant populations within the grounds of the hospital. Owing to this, we were summoned in September 2011. Thus, our challenge was to develop a protocol to control ants in the hospital that would improve the standards with commercial baits. In order to achieve that, we performed two different phases: 1) evaluation of the species present and some aspects of their behavior, and 2) control using baits.
Material and methods
The public pediatric hospital in this study occupies 3 hectares in the City of Buenos Aires (34° 35′ 39.20″ S; 58° 24′ 40.76″ W). At the site, there are around 18, 1 and 2 storey buildings interspersed with green spaces with different types of vegetation: trees, grass and ornamental plants. This hospital moved to these grounds in 1896. Several of the present buildings were constructed at that time. The hospital has 300 beds and approximately 2500 medical doctors and nurses work therein; thousands of people circulate there per day.
The objectives of this phase were to evaluate which species were present in green spaces, quantify their abundance, assess their food preferences and determine which were behaviorally dominant.
The individuals collected from manual sampling, pitfall traps and food baits outdoors and also those found indoors were used to make a list of species present in there. The genus abundance was quantified from pitfall traps, and behavioral dominance and food preferences were estimated by means of food baits. Samplings were performed from December 2011 to January 2012, which corresponds to late spring and summer in our latitude.
Six trained students toured the grounds sampling ants in the vegetation, grass, walls, pedestrian paths, and indoors, in order to identify the species and document which of those were associated with buildings. Samples were preserved in ethanol (96&) in bottles labeled with the capture site and date. Manual samplings were performed on two different days between 10 am and 3 pm. The only objective of this sampling was to contribute to the list of species present in the hospital.
24 non-baited pitfall traps were placed twice (two weeks apart) in different green spaces of the hospital and left for two days. Each trap consisted of a 200 ml plastic cup buried in the ground and half-filled with alcohol (70&). Pitfalls were left for 48 h, which allowed sampling ants with low activity at the times we did not have access to the hospital. Trapped ants were identified to species or genera with available keys (Kusnezov 1951 1978; MacKay and MacKay 2002; Fernández 2003; Wilson 2003). Relative abundance per genus was measured as the number of ants per genus over the total number of ants collected during the study.
Food sources were used in order to study the first genus in discovering the resource, the recruitment dynamics and the behavioral dominance among the different genera (Fellers 1987; Savolainen and Vepsäläinen 1988; Andersen 1997; Morrison et al. 2000). Four different types of food were offered simultaneously on “a station” (a square plastic sheet of 20 cm × 20 cm): commercial canned tuna in oil, commercial canned corned meat, honey-water and raw lean beef in similar amounts (ca. 1 g). Within the square sheet, each food was placed in the center of each quarter (i.e. 10 × 10 cm). A total of 32 baits were placed on 8 stations during 70 min. Every 10 min (from T0 = time 0 min to T7 = time 70 min), a photo of each bait was taken (Canon EOS 400D XTi, Canon EF50mm Compact macro lens); recordings and notes about the species visiting the baits were also made in parallel. Later in laboratory, the number of ants per species was counted per bait from the photos taken, in conjunction with ant samples taken from food bait stations.
Offering 4 alternative types of food per station also allowed us to evaluate food preferences for the species that visited these stations. The distribution of the different species that visited the 4 food baits was compared by means of Chi Squared Homogeneity test. Then, for each species, the number of ants foraging on each food bait was compared by means of Chi Squared Goodness of Fit Test in order to establish the preferences of each species.
The toxic baits
The bait consisted of sucrose solution (20& w/w) added with a toxicant offered in plastic tubes (5 ml and 10 mm diameter) with a plug of non-compacted cotton. It is important that the plug of cotton is set lightly so as not to dry on the outside, but does not drip when tilted down. This solution has low viscosity which is preferred for urban ant baits, because it does not present a mechanical difficulty for small species to ingest (Baker et al. 1985 Josens et al. 1998; Silverman and Roulston 2001; O’Brien and Hooper-Bui 2005; Medan and Josens 2005). The toxics used were two boron compounds: boric acid and borax (sodium borate). These boron toxicants are two of the few allowed by the toxicological service of the hospital, due to their very low toxicity for mammals.
Whenever possible, two baits were offered together: one containing boric acid (BA) and the other, borax (B). This decision was based on previous data on two species which presented the opposite rejection response toward those toxicants (Sola et al. 2013). As the response of the species to be found could not be predicted, offering two alternatives of boron compounds would increase the probability that one of them would be taken to the nest. If a given species rejected a particular boron compound, it would find and feed on the other.
Due to the constant presence of children, tubes had to be located in hidden or hard to reach places so as not to call their attention. Respecting this premise, tubes were placed as close to the ant trails as possible and fixed to the walls with tape (masking tape or duct tape) of a similar color to the background.
Highly infested pavilions and Focal infested rooms
Since the beginning of the cooperation with the hospital, its director instructed both the staff and the pest control personnel to notify us if ants were detected in the interior of the buildings.
Our first step in this control phase was to identify the pavilions highly infested with ants. For that, two kinds of recordings were considered: the detection of high ant activity on outer walls and the reports of the hospital staff from the rooms. The ant activity on outer walls was evaluated by means of an outside building visual survey for detection of ant trails by a group of 6 trained students who toured the site.
List of ant morphospecies detected in the hospital considering every sample method used: pitfall trapping, baits and manual sampling either inside the buildings or outside areas
Extremely rare. Individual ants on the grass, no trails were observed.
Abundant presence. Very small species; usually enters through tiny gaps and cracks in buildings or nests therein.
Abundant presence. Very small species; usually enters through tiny gaps and cracks in buildings or nests therein.
One of the most abundant species. Usually enter and nest in the buildings. Long trails alongside the outer walls of several pavilions.
Camponotus sp (prob. fuscocinctus):
Extremely rare. Arboreal, associated with green areas
Pheidole sp1 (cf P. rosae (Wilson))
Abundant in green areas. Not found associated to buildings.
Pheidole sp2 (cf P. radoszkowskii)
Abundant in green areas but rarely found inside buildings.
Pheidole acutiloba (Mackay)
Not abundant in green areas. It was found nesting in one pavilion.
Very small ants. They were found only on land and in the grass.
Black leaf-cutting ants, the biggest in the hospital. Nests are in green areas and also associated to buildings.
Very rare. Only in grass and trash. Very small ants.
Small ants. They were only detected in one building.
Low abundance. In green areas.
Low abundance. In green areas.
Low abundance. Arboreal, associated with green areas.
Relative abundance of genera
A total of 4,767 ants were collected in the 24 pitfall traps placed in the green areas of the hospital. Their genera and, when possible, the species were determined for each individual.
A more detailed observation of each sampling site revealed that Pheidole and Nylanderia were found together in each and every site (data not shown). That means they coexisted in all the green places and seemed not to be partitioned across the premises.
Visits and behavioral dominance at the bait stations
First genera to discover/recruit to baits
Time of Nf presence.> 3 workers*
Time of Nf recruit. > 20 workers**
Species (&) at T7 (time = 70 m)
Nylanderia ants discovered 5 out of the 8 stations, mostly, between 40 and 50 minutes after their placement and only then, recruited workers did begin to increase in number and the gradual dropping of Pheidole started. In some stations where Nylanderia arrived after 40 min, our recordings ended before this process was completed (see Station ST4 and ST8 in Table 2).
In conclusion, Nylanderia seems to be the behaviorally dominant ant due to their behavior of massive recruitment.
The different species that visited the baits have similar feeding habits, they are omnivorous and opportunistic. However, some differences were observed in the presence of ants of each genus among the four types of food baits offered (M eat, T una, H oney-water, C anned meat).
Considering each of these four species in particular, none of them visited the four food baits equally. Each species showed significant differences in the number of ants visiting the four food baits; that is at least one food type was either more or less visited than the others (Figure 4; Fit-Goodness Test: P < 0.001 for each species).
Although Nylanderia was found in all the foods offered, the sugary bait was the most visited and showed the fastest recruitment; tuna was preferred over the canned meat. In the case of Pheidole, the tuna bait was the least visited by the two species of Pheidole mostly represented (P. sp1 and P. sp2, cf. P. rosae and cf. P. radoszkowskii, respectively). P. sp1 showed a preference for honey-water and canned meat, while P. sp2 mainly visited canned meat over the other baits. P. acutiloba (Mackay et al. 2011) visited the honey-water significantly less compared to the other baits.
Brachymyrmex and Solenopsis were found visiting the baits in low numbers. Solenopsis albidula was found in two stations visiting at least once the Meat, Tuna and Canned meat, but with a clear preference for the meat. Brachymyrmex patagonicus was observed in only one honey-water bait.
Even though we only had 8 stations, the patterns of preferences described in the previous lines were consistent. The same pattern was observed when considering all the stations together (Figure 3), only the station where each Pheidole species was dominant (Table 2: Station ST6 for P. acutiloba; Station ST3 for P. sp1; Station ST7 for P. sp2), or even considering only the times that each species was dominant in the stations (i.e. “before” Nylanderia arrived).
Many details of the protocol to be developed indoors were determined after trying different alternatives in laboratory and from our previous research. In general terms, the protocol followed some guidelines.
As a general rule to be applied in each case, some individual ants were sampled in order to identify the species and conserved in alcohol (96&) in flasks labeled with the capture site and date.
Regarding the collocation of the bait tubes, they were placed horizontally to ensure that the cotton plug was wet as long as possible. To promote the ants to find the mouth of the tube rapidly, a line of sugar solution was painted by means of a thin brush so that it connected each tube mouth with the ant trail. The procedure described above was followed on every occasion: two tubes close to each other were placed with two boron baits -whenever possible- in hidden places, attached horizontally with tape of the background color, etc. (see Materials and Methods).
Highly infested pavilions
The reports received from the hospital staff about ants found in rooms revealed that most of them were located on the ground floor and that several buildings along the premises were highly infested.
Several very active trails in the outer walls were found from the outside building visual survey. Particularly, in three buildings where ants walking on the edge of a wall always entered the building, either through a window or through small cracks or holes in the wall.
As a general pattern, there was a correspondence between the buildings with a lot of reports in rooms and buildings with high level of activity outside.
For the severely infested pavilions, our criterion was to set the outside baits all around the buildings, as it is better to attract ants outward rather than inward.
Following the described procedure, firstly we surrounded 3 pavilions (N, P and F) with an extensive outer baiting where children did not circulate or access. That is to say, there were walls with a lot of tubes because children had no access, and sections of walls that had no tubes at all because children were all around. Moldings or baseboards were used to support and fix the tubes as explained above. In Pavilion N, 28 tubes were placed (14 with BA and 14 with B) and for all those tubes and throughout the days recorded, the only species observed was Nylanderia fulva. In Pavilion P, 30 tubes were placed (15 BA and 15 B) and the most abundant species was also N. fulva. In Pavilion F, 20 tubes were placed (10 BA and 10 B) and the species recorded were N. fulva, one species of Brachymyrmex and the leaf cutting ant Acromyrmex lundi.
As the weather was turning cooler, Nylanderia ants were particularly abundant close to the heating-pipe outlets, a fact that is likely to be accentuated during the winter. In early May 2012, the temperature next to these outlets was 30°C (recorded on the wall next to the ant trail with a laser thermometer), while on other walls was about 20°C.
In these pavilions Nylanderia commonly presented active trails up to ca. 20 meters long bordering the outer walls. Ants entered and exited from the building through different places, either small holes or the windows. This suggests a high connectivity between colonies with a unicolonial structure, which is common in this species.
The extensive outer baiting lasted a month and was highly effective in reducing the Nylanderia infestation indoors in all the pavilions. After this, no more N. fulva ants were detected in rooms with outer walls and in the outside for several months.
Focal infested rooms
In those focal rooms where ants were detected by the hospital staff, the entrance hole or crack inside the room through which the ants came in was searched. That was an easy task if there was high ant activity. Otherwise, if there were no ants at the moment of this evaluation, small drops of sugar-water were spread on the counters near the corners where the ants had previously been seen. Then, the first ants that appeared were followed to spot the entry. After that, the drops were cleaned.
Then, it was evaluated if the entrance hole was located in walls that limited the building to the outside or in inner walls.
In case of external walls, whenever possible, ant activity on the outside was searched, particularly in the sector that matched the indoor ant detection. If the same type of ant was on both sides of the wall, we assumed they belonged to the same colony. Following the premise that it was better to attract ants outward rather than inward, baits were placed firstly on the outside.
In case of inner walls, whenever possible, ant activity on the other side of the wall was searched. If the same type of ant was also there, then we chose the less sensitive room for bait placement. Thereafter, small cracks and fissures of the more sensitive room were sealed, since the ants left for the baits in the other room. Only in cases when the other side of the wall had no ants or was inaccessible, a bait station in the affected room was set.
We were very careful in placing the baits as close as possible to the hole where the ants came out; otherwise, they could establish trails on the walls or across the countertops, which clearly had to be avoided, especially if patients were children.
Indoor sites treated with sugar toxic baits
Nº of tubes: baits applied
Result + / -
Individual Room 4, U5
Individual Room 2, U5
Individual Room 3, U5
2: BA, B
Individual Room B, IC and office
2: BA, B
Individual Room A IC
2: BA, B
Individual Room A IC
2: BA, B
Resident physician’s Bathroom, U5
2: BA, B
2: BA, B
Individual Room 4, U10
Individual Room 10, U9
2: BA, B
Laboratory and office
4: BA, B
General room and nurse office
10: BA, B
6: BA, B
The most frequent species indoors were Nylanderia fulva, Brachymyrmex patagonicus and B. australis. Out of the 13 rooms affected by ants, 6 corresponded to N. fulva (46&). The activity of these ants was always particularly intense. The delay or days until non-detection (Delay in Table 3) ranged from 5 to 21 days. Four rooms were affected by Brachymyrmex (31&): two by B. patagonicus and two by B. australis. In all cases the treatment was effective, ants were no longer detected between the first and second week after placing the baits.
One site (11) had very intense and constant activity of the small Pharaoh ants, Monomorium pharaonis. This laboratory consisted of 3 rooms, 2 of which were the only places in the hospital where this species was found. Two tubes (1 with BA and 1 with B) were set in each of these two rooms, and a week later no more ants were observed.
Pheidole sp1 was detected only once indoors (site 4), and this small colony showed no further activity after 5 days of treatment.
We followed up the rooms treated, both by visual assessment and by querying the staff working therein about ant detection. In all the cases mentioned above, no ants were observed in the following seven months. It is worth mentioning that in any case when ants disappeared from one place, they never appeared in a proximal other place, i.e. out of this first control experience, there was no evidence that the baiting provoked the ants to move from one place to another.
One of the two species in which the treatment was not effective was Acromyrmex lundi (leaf-cutting ants). They regularly visited the baits during the outer baiting first and then in indoor baiting. However, it is known that this species is not specialized in sugary solutions but forage for fresh plant material they need as substrate for their symbiotic fungus. For this species, there are commercial granulated baits.
The other species that could not be controlled was P. acutiloba. This case required special work and attention because two nests were in a very sensitive area. For the first nest detected, the baits were controlled three times per week and the solutions were replaced once a week at least for a month and it still showed signs of activity. Meanwhile, we observed the ants’ behavior. The activity of this colony was always very low: only one to three individuals came out of the mouth of the nest, and walked slowly without straying more than a few centimeters from it. The furthest they were seen was ca. 20 cm from the mouth. The tubes with the sugar baits were initially placed on the molding that formed a curve between the wall and the floor of the room. In one observation, we noticed that some ants had difficulties climbing over this curved area as they slipped down. Consequently, the tubes were placed at the ground level. Despite our efforts, this nest could not be controlled, and maintained a low level of activity, which could be seen not only by the observation of individual ants walking around, but also by construction material frequently deposited in a semicircle around the mouth of the nest.
Considerations regarding the developed protocol
The protocol showed to be highly effective for the ants that most frequently nested in the hospital buildings (Nylanderia and Brachymyrmex). Combining two different toxicants –boric acid and borax- has proved to be particularly effective, since in all cases tested, each species visited one toxicant more than the other (data not shown).
The results were evident for patients and hospital staff. In all the sites where control measures were applied, no ants were observed in the following seven months. No further action was taken on those sites, outside or inside. Only until March and April 2013, when summer ended, some of the treated rooms did show ants again. The species were the same as those identified one year earlier; a fact that was recorded for N. fulva, Brachymyrmex and Monomorium. This suggests that the nests were not entirely eliminated, but instead the population dropped. In 2013, the same protocol was applied in the sites where ants resurged and they disappeared once more. Based on this result - and as expected- once or twice annual maintenance applications are recommended at key moments, maybe at the end of summer or early fall. In this temperate climate, where winter has temperatures than can reach zero degrees Celsius (°C), ants tend to congregate in hot spots and heated buildings.
It is often difficult to locate ant nests, but with this protocol it is not necessary. External baiting was effective for the most abundant ant N. fulva; as placing baits outside the affected buildings resulted in a dramatic reduction in activity inside. These results coincide with previous studies involving Pharaoh ants (Haack 1991; Oi et al. 1994) and Argentine ants (Forschler and Evans 1994). It was suggested that a toxicant bait formulation could be simply scattered around the exterior periphery of a building, which would be easier to use than placing and removing bait stations (Oi et al. 1996). Even when bait station arrangement and refilling would be harder, certainly the effect of evaporation in hot weather is lower when the solution is within a tube than when scattered. On the other hand, baiting or scattering all along the perimeter is not applicable to sensitive areas such as pediatric hospitals because of the restrictions imposed by the children’s presence. There, bait stations required to be localized in place where children had no access, along the perimeter. Nonetheless, it proved to be effective.
Considerations regarding the hospital
In relation to the situation of the hospital, there are several issues that hinder ant control within buildings.
The buildings had many small holes that allowed ants enter the walls where they could nest and then exit in the inside. In this sense it was important for us to seal the smaller holes by using silicone and also to work together with the maintenance staff in order to repair deteriorated walls in some sensitive rooms.
The patients’ visitors’ habits seem to be difficult to control. On several occasions we detected ants collecting food in garbage thrown into the bathroom waste basket. Even though visitors are aware that this induces insects to enter the rooms, as indicated by nurses, they state that it is easier for them to do so. It is difficult to change these habits, thus, it should be important to implement some measures in order to increase the visitors’ awareness of the problem or even evaluate an alternative measure so that they can get rid of food wastes in the rooms in a safer way to prevent insect attraction.
In this survey on the ant species present in the hospital, we found 7 different ant species indoor and a total of 15 species considering both indoor and green areas. Several studies have demonstrated a great variability of ant assemblage richness and species composition associated with human structures in Brazil, even in hospitals (Fowler et al. 1993; Bueno and Fowler 1994). This is unlike the findings of studies reported from Europe and North America, which have few associated species. It is natural to expect a small number of species in hospitals of temperate climates (Fowler et al. 1993). However, we have found in a hospital of Buenos Aires a similar number of species to several hospitals of Brazil, despite our temperate climate.
It is possible that for some species, the observed activity was low because the ants had different time patterns, being more active at night, dusk or dawn rather than in the middle of the day. However, the pitfall traps could collect and show such an activity as they were left for 48 h. Besides, hospital staff could detect and report ant activity indoors at any time of the day.
Nylanderia fulva resulted to be the main species in association with the buildings. In green areas throughout the hospital we saw the coexistence of N. fulva with Pheidole, Brachymyrmex and Solenopsis. This coexistence had previously been observed in other urban environments (McDonald 2012). Nylanderia accepted all food baits, but the most visited was the sugary bait. This is in accordance with other studies, where sugar based baits were preferred over protein in the laboratory (Cook et al. 2011) and in the field (Lynch et al. 1980). Another study found that N. fulva was apparently not attracted to oil based baits (Drees et al. 2010); however, tuna in oil was the second most accepted bait in this study.
Nylanderia was typically found in trash cans and restroom drains, but it was also observed in the beds of patients. Oral reports from nurses indicated that if dextrose serum leaked during routine handling, ants would subsequently appear.
A recent study on N. fulva (McDonald 2012) mentioned that it is not attracted to most commercial ant baits but one granular product. Some treatments involving insecticides applied to surfaces provided temporary buffer zones around structures, only partially controlling N. fulva. Field assessments of the only commercial bait that N. fulva found attractive was not effective for control: reduction in the number of ants was low, and after the third or fourth week numbers returned to levels prior to treatment. Although other authors (Zenner-Polania 1990) concluded that effective control of N. fulva could not be accomplished through a single control method, we found that our bait and application method were sufficient to control localized populations for up to 7 months. We do not disregard the fact that in temperate climates, harsh winters can work in favor of extending control effectiveness.
Brachymyrmex was observed in kitchens, offices, laundry rooms; also in neonatal units. These ants showed an intense activity and quick visits to the baits once placed. Brachymyrmex patagonicus was described as an invader ant in the USA, where it nests in natural and disturbed areas, and it is also found in very high numbers in hospitals and other buildings (MacGown et al. 2007). Its diet consists largely of honeydew of different insects, so it is expected to readily recruit to sweet baits. In fact, this was observed in the food bait stations where they were detected only in the sugary bait and also in the effectiveness of the toxic sugar bait used for control. Brachymyrmex ants have been found as the most frequent species in some hospitals in Porto Alegre, Brazil (Garcia et al. 2011).
Monomorium pharaonis, the Pharaoh ants, were detected only indoors, which coincides with observations for this species which nests primarily indoors in temperate regions (Ebeling 1978). The sugar toxic baits were well accepted and promptly effective. These ants compensate for what they are lacking nutritionally by foraging on either carbohydrates or proteins (Edwards and Abraham 1990). M. pharaonis was dominant in several hospitals in Brazil (Fowler et al. 1993; Lise et al. 2006), as well as in temperate climates such as in England (Edwards and Baker 1981). Nevertheless, we only found this species in one laboratory of our hospital in Buenos Aires.
Generally speaking, Pheidole is a very diverse genus composed mainly of omnivorous ants. Some groups of species were characterized as specialized, either strict granivores or carnivores (Kusnezov 1951). Carnivores can forage on dead or alive insects but also feed on sweet liquids such as honeydew or extrafloral nectars (Blom and Clark 1980). However, P. grallipes that was thought to be exclusively entomophagus, resulted to have a more diversified diet (Clark et al. 1986), and two other Pheidole from Argentina also revealed that they were less specialized than expected (Pirk et al. 2009). In a hospital in Brazil, a Pheidole ant visited equally sugar and protein baits (Pesquero et al. 2008), which coincides with two species of Pheidole found in our study which collected protein and sugar bait as well.
P. acutiloba preferred the sugary baits less than the protein baits. Considering that sugar was not a main resource for this ant, in the indoor control situation, after some weeks of no response toward the boron baits, we tried a mixed amino acid-sucrose solution; however, no signs of ants drinking on it were observed either. Therefore, it is possible that this species avoids liquid baits. A study on a laboratory colony of a Pheidole species offering simultaneously 4 different vehicles for 30& sucrose bait, suggested a preference for dry vehicles (Lee 2008).
As mentioned above, P. acutiloba proved to be the only species among those observed on the food bait stations that visited significantly less the sugary bait than the other foods. This result could explain the low effectiveness of the toxic bait used with this particular species, confirming the need to consider the food preferences of the target species for bait formulations. Eichler (1990) suggested the use of “pre-baits” for M. pharaonis in order to determine their preferences; this recommendation is valid for many species, especially for those whose feeding habits are unknown.
In summary, more and more studies conclude that it is necessary to consider the behavior and nutritional preferences of the target species, and also the toxic to be used for the baits when designing control programs (Lee 2008; Sola et al. 2013). For the species where sugary baits did not work, a bait-preference test could indicate the most appropriate bait to use in that particular case. In this sense, we are still working on increasing the acceptance of sugar toxic baits in situations where they are not well accepted.
As mentioned previously, the fact that the species and locations were the same from one year to the next shows that total eradication of the pest ants was not possible in this instance. However, we were able to control the populations of problematic ants eliminating their detection from inside buildings. Based on this, we suggest consistent and regular population monitoring and the use of an integrated management to achieve proper levels of pest control.
We would like to thank the Hospital Authorities, medical and general staff for their support and kindness. Special thanks to Isabel Ortega Insaurralde, Tomás Righetti, Pablo Jusim for their collaboration in outdoor tasks at the hospital.
This study was supported by the National Agency for Scientific and Technological Promotion (PICT 2007–01319) and the National Council for Scientific and Technological Research of Argentina.
All necessary permits were obtained for the described study, which complied with all relevant regulations. The species we worked on are not endangered or protected. We had the permissions required for these activities in the hospital from the corresponding authorities.
- Alexander FE, Patheal SL, Biondi A, Brandalise S, Cabrera ME, Chan LC, Chen Z, Cimino G, Cordoba JC, Gu LJ, Hussein H, Ishii E, Kamel AM, Labra S, Magalhães IQ, Mizutani S, Petridou E, Pombo de Oliveira M, Yuen P, Wiemels JL, Greaves MF: Transplacental chemical exposure and risk of infant leukemia with MLL gene fusion. Cancer Res 2001, 61: 2542-2546.Google Scholar
- Andersen AN: Functional groups and patterns of organization in North American ant communities: a comparison with Australia. J Biogeogr 1997, 24: 433-460. 10.1111/j.1365-2699.1997.00137.xView ArticleGoogle Scholar
- Bailey HD, Armstrong BK, de Klerk NH, Fritschi L, Attia J, Scott RJ, Smibert E, Milnel E: Exposure to professional pest control treatments and the risk of childhood acute lymphoblastic leukemia. Int J Cancer 2011, 129: 1678-1688. 10.1002/ijc.25769View ArticleGoogle Scholar
- Baker TC, Van Vorhis Key SE, Gaston LK: Bait-preference tests for the Argentine ant (Hymenoptera: Formicidae). J Econ Entomol 1985, 78: 1083-1088.View ArticleGoogle Scholar
- Beatson SH: Pharaoh’s ants as pathogen vectors in hospitals. Lancet 1972, 1: 425-427.View ArticleGoogle Scholar
- Blom PE, Clark WH: Observations of ants (Hymenoptera: Formicidae) visiting extrafloral nectaries of the barrel cactus Ferrocactus gracilis (Cactaceae) in Baja California, Mexico. Southwest Nat 1980, 25: 181-196. 10.2307/3671240View ArticleGoogle Scholar
- Buczkowski G, Scharf ME, Ratliff CR, Bennett GW: Efficacy of simulated barrier treatments against laboratory colonies of Pharaoh ant. J Econ Entomol 2005, 98: 485-492. 10.1603/0022-0493-98.2.485View ArticleGoogle Scholar
- Bueno OC, Campos-Farinha AEC: As formigas domésticas. In Insetos e outros invasores de residências. Edited by: Mariconi FAM. Piracicaba: Fundação de Estudos Agrários Luiz de Queiroz; 1999:135-180.Google Scholar
- Bueno OC, Fowler HG: Exotic ants and native ant fauna of Brazilian hospitals. In Exotic ants – Biology, impact and control of introduced species. Edited by: Williams DF. Boulder: Westview Press; 1994:191-198.Google Scholar
- Cassill DL, Tschinkel WR: Regulation of diet in the fire ant, Solenopsis invicta . J Insect Behav 1999, 12: 307-328. 10.1023/A:1020835304713View ArticleGoogle Scholar
- Clark WH, Blom PE, Lownam AM: Contents of a nest of the desert ant, Pheidole grallipes wheeler, in Baja California, Mexico. Pan-Pac Entomol 1986, 62: 99-102.Google Scholar
- Cook S, Wynalda R, Gold R, Behmer S: Macronutrient regulation in the Rasberry crazy ant ( Nylanderia sp. nr. pubens ). Insect Soc 2011, 59: 1-8.Google Scholar
- Dornhaus A, Collins EJ, Dechaume-Moncharmont FX, Houston AI, Franks NR, McNamara JM: Paying for information: partial loads in central place foragers. Behav Ecol Sociobiol 2006, 61: 151-161. 10.1007/s00265-006-0246-5View ArticleGoogle Scholar
- Drees BM, McDonald DL, Calixto A, Nester P, Bowen C, Rasberry T, Gold R 2010 IPM Handbook. Evaluation of bait attractiveness for the Rasberry crazy ant 2010, 93-95.Google Scholar
- Ebeling W: Urban Entomology. Berkeley: Division of Agricultural Sciences University of California; 1978.Google Scholar
- Edwards JP, Abraham L: Changes in food selection by workers of the Pharaoh’s ant, Monomorium pharaonis . Med Vet Entomol 1990, 4: 205-211. 10.1111/j.1365-2915.1990.tb00278.xView ArticleGoogle Scholar
- Edwards JP, Baker LF: Distribution and importance of the Pharaoh’s ant Monomorium pharaonis (L.) in National Health Service hospitals in England. J Hosp Infect 1981, 2: 249-254.View ArticleGoogle Scholar
- Eichler W: Health aspects and control of Monomorium pharaonis . In Applied Myrmecology: A World Perspective. Edited by: Vander Meer RK, Jaffe K, Cedeno A. Boulder: Westview Press; 1990:671-675.Google Scholar
- Falibene A, Josens R: Nectar intake rate is modulated by changes in sucking pump activity according to colony starvation in carpenter ants. J Comp Physiol A 2008, 194: 491-500. 10.1007/s00359-008-0323-5View ArticleGoogle Scholar
- Falibene A, Figueiredo Gontijo A, Josens R: Sucking pump activity in feeding behaviour regulation in carpenter ants. J Insect Physiol 2009, 55: 518-524. 10.1016/j.jinsphys.2009.01.015View ArticleGoogle Scholar
- Fellers JH: Interference and exploitation in a guild of woodland ants. Ecology 1987, 69: 1466-1478.View ArticleGoogle Scholar
- Fernández F: Introducción a Las Hormigas de la Región Neotropical. Bogotá: Instituto de Investigación de Recursos biológicos Alexander von Humboldt; 2003.Google Scholar
- Forschler BT, Evans GM: Perimeter treatment strategy using containerized baits to manage Argentine ants, Linepithema humile (Mayr) (Hymenoptera: Formicidae). J Entomol Sci 1994, 29: 264-267.Google Scholar
- Fowler HG, Bueno OC, Sadastsune T, Montelli A: Ants as potential vectors of pathogens in hospitals in the state of São Paulo, Brazil. Int J Trop Insect Sci 1993, 14: 367-370. 10.1017/S1742758400014879View ArticleGoogle Scholar
- Garcia FRM, Ahlert CC, de Freitas BR, Trautmann MM, Tancredo SP, Lutinski JA: Ants (Hymenoptera: Formicidae) in five hospitals of Porto Alegre, Rio Grande do Sul State, Brazil. Acta Scientiarum Health Sci 2011, 33: 203-209.View ArticleGoogle Scholar
- Haack KD: Elimination of Pharaoh ants, an analysis of field trials with Pro-Control and Maxforce ant baits. Pest Control Technol 1991, 19: 32-33.Google Scholar
- Hansen LD, Klotz JH: Carpenter Ants of the United States and Canada. Ithaca, NY: Cornell Univ. Press; 2005.Google Scholar
- Hedges SA: Field Guide for the Management of Structure – Infesting Ants. Richfield: GIE Inc Publishers; 2010.Google Scholar
- Herin F, Boutet-Robinet E, Levant A, Dulaurent S, Manika M, Galatry-Bouju F, Caron P, Soulat JM: Thyroid function tests in persons with occupational exposure to fipronil. Thyroid 2011, 21: 701-706. 10.1089/thy.2010.0449View ArticleGoogle Scholar
- Hooper-Bui LM, Rust MK: An oral bioassay for the toxicity of hydramethylnon to individual workers and queens of Argentine ants, Linepithema humile . Pest Manag Sci 2001, 57: 1011-1016. 10.1002/ps.378View ArticleGoogle Scholar
- Howard DF, Tschinkel WR: The effect of colony size and starvation on food flow in the fire ant, Solenopsis invicta (Hymenoptera: Formicidae). Behav Ecol Sociobiol 1980, 7: 293-300. 10.1007/BF00300670View ArticleGoogle Scholar
- Infante-Rivard C, Labuda D, Krajinovic M, Sinnett D: Risk of childhood leukemia associated with exposure to pesticides and with gene polymorphisms. Epidemiology 1999, 10: 481-489. 10.1097/00001648-199909000-00004View ArticleGoogle Scholar
- Josens R, Roces F: Foraging in the ant Camponotus mus : nectar intake rate and crop filling depend on colony starvation. J Insect Physiol 2000, 46: 1103-1110. 10.1016/S0022-1910(99)00220-6View ArticleGoogle Scholar
- Josens R, Farina WM, Roces F: Nectar feeding by the ant Camponotus mus : intake rate and crop filling as a function of sucrose concentration. J Insect Physiol 1998, 44: 579-585. 10.1016/S0022-1910(98)00053-5View ArticleGoogle Scholar
- Kapoor U, Srivastava MK, Srivastava LP: Toxicological impact of technical imidacloprid on ovarian morphology, hormones and antioxidant enzymes in female rats. Food Chem Toxicol 2011, 49: 3086-3089. 10.1016/j.fct.2011.09.009View ArticleGoogle Scholar
- Klotz JH: Liquid bait delivery systems for controlling Argentine ants in citrus groves (Hymenoptera: Formicidae). Sociobiol 2004, 43: 419-427.Google Scholar
- Klotz JH, Greenberg L, Amrhein C, Rust M: Toxicity and repellency of borate-sucrose water baits to Argentine ants (Hymenoptera: Formicidae). J Econ Entomol 2000, 93: 1256-1258. 10.1603/0022-0493-93.4.1256View ArticleGoogle Scholar
- Klotz JH, Hansen L, Pospischil R, Rust M: Urban Ants of North America and Europe. Identification, Biology and Management. Ithaca: Cornell University Press; 2008.Google Scholar
- Klotz JH, Hansen L, Field H, Rust M, Oi D, Kupfer K: Urban Pest Management of Ants in California. Richmond: Division of Agricultural and Natural Resources University of California; 2010.Google Scholar
- Kusnezov N: El Género “ Pheidole ” en la Argentina (Hymenoptera Formicidae). Acta Zool Lilloana 1951, 12: 5-88.Google Scholar
- Kusnezov N: Hormigas argentinas – Clave para su identificación. Fundación Miguel Lillo. Tucumán: Miscelánea 61; 1978.Google Scholar
- Lee CY: Sucrose bait base preference of selected urban pest ants (Hymenoptera: Formicidae). In Proceedings of the Sixth International Conference on Urban Pests. Edited by: Robinson WH, Bajomi D. Veszprém: OOK-Press Kft; 2008:59-63.Google Scholar
- Leiss JK, Savitz DA: Home pesticide use and childhood cancer: a case–control study. Am J Public Health 1995, 85: 249-252. 10.2105/AJPH.85.2.249View ArticleGoogle Scholar
- Lise F, Garcia FRM, Lutinski JA: Association of ants (Hymenoptera: Formicidae) with bacteria in hospitals in the State of Santa Catarina. Rev Soc Bras Med Trop 2006, 39: 523-526. 10.1590/S0037-86822006000600002View ArticleGoogle Scholar
- Lynch JF, Balinsky EC, Vail SG: Foraging patterns in three sympatric forest ant species, Prenolepis imparis , Paratrechina melanderi and Aphaenogaster rudis (Hymenoptera: Formicidae). Ecol Entomol 1980, 5: 353-371. 10.1111/j.1365-2311.1980.tb01160.xView ArticleGoogle Scholar
- Ma X, Buffler PA, Gunier RB, Dahl G, Smith MT, Reinier K, Reynolds P: Critical windows of exposure to household pesticides and risk of childhood leukemia. Environ Health Perspect 2002, 110: 955-960. 10.1289/ehp.02110955View ArticleGoogle Scholar
- MacGown JA, Hill JG, Deyrup MA: Brachymyrmex patagonicus (Hymenoptera: Formicidae), an emerging pest species in the southeastern United States. Fla Entomol 2007, 90: 457-464. 10.1653/0015-4040(2007)90[457:BPHFAE]2.0.CO;2View ArticleGoogle Scholar
- MacKay WP, MacKay E: The ants of New Mexico (Hymentoptera: Formicidae). Lewiston: Edwin Mellen Press; 2002.Google Scholar
- Mackay WP, Sola FJ, Josens R: A new shield-faced ant of the genus Pheidole (Hymenoptera: Formicidae: aberrans Group) from Argentina. Trans Am Entomol Soc 2011, 137: 297-305.Google Scholar
- Mailleux AC, Detrain C, Deneubourg JL: Starvation drives a triggering communication. J Exp Biol 2006, 209: 4224-4229. 10.1242/jeb.02461View ArticleGoogle Scholar
- Mallis A: Handbook of Pest Control. New York: MacNair-Dorland Co.; 1969.Google Scholar
- Markin GP: Foraging Behavior of the Argentine Ant in a California Citrus Grove. J Econ Entomol 1970, 63(3):740-744.View ArticleGoogle Scholar
- McDonald DL Doctoral dissertation. In Investigation of an invasive ant species: Nylanderia fulva. Colony extraction, management, diet preference, fecundity, and mechanical vector potential. Texas A & M University; 2012.Google Scholar
- Medan V, Josens R: Nectar foraging behaviour is affected by ant body size in Camponotus mus . J Insect Physiol 2005, 51: 853-860. 10.1016/j.jinsphys.2005.03.016View ArticleGoogle Scholar
- Menegaux F, Baruchel A, Bertrand Y, Lescoeur B, Leverger G, Nelken B, Sommelet D, Hémon D, Clavel J: Household exposure to pesticides and risk of childhood acute leukaemia. Occup Environ Med 2006, 63: 131-134. 10.1136/oem.2005.023036View ArticleGoogle Scholar
- Moreira DDO, Morais V, Vieira-da-Motta O, Campos-Farinha AEC, Tonhasca JA: Ants as carriers of antibiotic-resistant bacteria in hospitals. Neotrop Entomol 2005, 34: 999-1006. 10.1590/S1519-566X2005000600017View ArticleGoogle Scholar
- Morrison LW, Kawazoe EA, Guerra R, Gilbert LE: Ecological Interactions of Pseudacteon parasitoids and Solenopsis ant hosts: environmental correlates of activity and effects on competitive hierarchies. Ecol Entomol 2000, 25: 433-444. 10.1046/j.1365-2311.2000.00280.xView ArticleGoogle Scholar
- Newell W, Barber TC: The Argentine ant. US Dept Agric Bureau of Entomology Bulletin; 1913:122.Google Scholar
- O’Brien KS, Hooper-Bui LM: Hunger in red imported fire ants and their behavioral response to two liquid bait products. J Econ Entomol 2005, 98: 2153-2159. 10.1603/0022-0493-98.6.2153View ArticleGoogle Scholar
- Oi DH: Pharaoh ants and fire ants. In Public Health Significance of Urban Pests. Edited by: Bonnefoy X, Kampen H, Sweeney K. Copenhagen: WHO; 2008:175-208.Google Scholar
- Oi DH, Vail KM, Williams DF, Bieman DN: Indoor and outdoor foraging locations of Pharaoh ants (Hymenoptera: Formicidae) and control strategies using bait stations. Fla Entomol 1994, 77: 85-91. 10.2307/3495874View ArticleGoogle Scholar
- Oi DH, Vail KM, Williams DF: Field evaluation of perimeter treatments for Pharaoh ant (Hymenoptera: Formicidae) control. Fla Entomol 1996, 79: 252-263. 10.2307/3495822View ArticleGoogle Scholar
- Olaya-Masmela LA, Chacón de Ulloa P, Payán A: Hormigas (Hymenoptera: Formicidae) en centros hospitalarios del Valle del Cauca como vectores de patógenos nosocomiales. Rev Colomb Entomol 2005, 31: 183-187.Google Scholar
- Owens K: Healthy Hospitals: Controlling Pest Without Harmful Pesticides. Washington: Beyond Pesticides; 2003.Google Scholar
- Pampiglione G, Velo E: Pest control in Albania: an example of collaboration in technical and scientific development in public health. Vet Ital 2010, 46: 37-43.Google Scholar
- Pesquero MA, Elias-Filho J, Carneiro LC, Feitosa SB, Oliveira MA, Quintana RC: Formigas em ambiente hospitalar e seu potencial como transmissoras de bactérias. Neotrop Entomol 2008, 37: 472-477. 10.1590/S1519-566X2008000400017View ArticleGoogle Scholar
- Pirk GI, Lopez de Casenave J, Pol R, Marone L, Milesi F: Influence of temporal fluctuations in seed abundance on the diet of harvester ants ( Pogonomyrmex spp.) in the central Monte desert, Argentina. Austral Ecol 2009, 34: 908-919. 10.1111/j.1442-9993.2009.01999.xView ArticleGoogle Scholar
- Pombo-de-Oliveira MS, Koifman S: Infant acute leukemia and maternal exposures during pregnancy. Cancer Epidemiol Biomarkers Prev 2006, 15: 2336-2341. 10.1158/1055-9965.EPI-06-0031View ArticleGoogle Scholar
- Rudant J, Menegaux F, Leverger G, Baruchel A, Nelken B, Bertrand Y, Patte C, Pacquement H, Vérité C, Robert A, Michel G, Margueritte G, Gandemer V, Hémon D, Clavel J: Household exposure to pesticides and risk of childhood hematopoietic malignancies: the ESCALE study (SFCE). Environ Health Perspect 2007, 115: 1787-1793. 10.1289/ehp.10596View ArticleGoogle Scholar
- Santos PF, Fonseca AR, Sanchez NM: Formigas (Hymenoptera: Formicidae) como vetores de bactérias em dois hospitais do município de Divinópolis, Estado de Minas Gerais. Rev Soc Bras Med Trop 2009, 42: 565-569. 10.1590/S0037-86822009000500016View ArticleGoogle Scholar
- Savolainen R, Vepsäläinen K: A competition hierarchy among boreal ants: impact on resource partitioning and community structure. Oikos 1988, 51: 135-155. 10.2307/3565636View ArticleGoogle Scholar
- Shannon WM, Borron SW, Burns MJ: Haddad and Winchester’s Clinical Management of Poisoning and drug overdose. Philadelphia: WB Saunders Company; 2007.Google Scholar
- Silverman J, Roulston TH: Acceptance and intake of gel and liquid sucrose compositions by the Argentine ant (Hymenoptera: Formicidae). J Econ Entomol 2001, 94: 511-515. 10.1603/0022-0493-94.2.511View ArticleGoogle Scholar
- Sola F, Falibene A, Josens R: Asymmetrical behavioral response toward two boron toxicants depends on the ant species (Hymenoptera: Formicidae). J Econ Entomol 2013, 106: 929-938. 10.1603/EC12246View ArticleGoogle Scholar
- Thompson JR, Gerald PF, Willoughby ML, Armstrong BK: Maternal folate supplementation in pregnancy and protection against acute lymphoblastic leukaemia in childhood: a case–control study. Lancet 2001, 358: 1935-1940. 10.1016/S0140-6736(01)06959-8View ArticleGoogle Scholar
- Wilson EO: Pheidole in the New World: a dominant, hyperdiverse ant genus, vol 1. Cambridge, USA: Harvard University Press; 2003.Google Scholar
- Winans B, Humble MC, Lawrence BP: Environmental toxicants and the developing immune system: a missing link in the global battle against infectious disease? Reprod Toxicol 2011, 31: 327-336. 10.1016/j.reprotox.2010.09.004View ArticleGoogle Scholar
- Zenner-Polania I: Biological aspects of the “Hormiga Loca” Paratrechina ( Nylanderia ) fulva (Mayr), in Colombia. In Applied Myrmecology: A World Perspective. Edited by: Vander Meer RK, Jaffe K, Cedeno A. Boulder: Westview Press; 1990:290-297.Google Scholar
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