Figure 5

The effect of p31 ^comet overexpression on drug-induced apoptotic cell death. (a) Apoptosis analysis of p31^comet-overexpressing cells with continuous treatment by anti-mitotic drugs. HeLa cells overexpressing EGFP or EGFP-p31^comet and treated with anti-mitotic drugs were subject to apoptosis analysis with MultiCaspase detection kit. (b) Cell survival analysis of p31^comet overexpressing cells with continuous treatment by anti-mitotic drugs. HeLa cells overexpressing EGFP or EGFP-p31^comet and treated with anti-mitotic drugs were subject to trypan blue exclusion to counte live cells at the indicated time points. (c) The FACS analysis of p31^comet-overexpressing HCT116 cells. HeLa or HCT116 cells were infected with EGFP or EGFP p31^comet adenovirus for 24 h, and the viruses were washed out with fresh medium. The infected cells were incubated with or without nocodazole for 24 h and prepared for FACS analysis. A population of >4 N DNA content in the cells was shown.