Figure 8

HPTLC of proteoglycans. The proteoglycans chondroitin sulfate (CS) and heparan sulfate (HS) were applied at 10 μg/spot to an HPTLC silica gel 60 plate. The plate was developed in chloroform:methanol:0.2%CaCl₂::55:45:10 and stained with resorcinol as described for the plate shown in Figure 6D. The proteoglycans failed to migrate from the origin and stained poorly with resorcinol, in contrast to the GD1a ganglioside standard and with the antigen recognized by MAb 10A5.