Figure 2
Immunoblot analysis of lens membrane fractions isolated from 75-day-old rats. (A) Lens membrane fractions separated by electrophoresis through 18% polyacrylamide gels were transferred to PVDF and immunoblotted with MAb 10A5. The NSMF and 25/45 fraction were applied at 5 ÎĽg/lane. (B) A duplicate gel was stained with Coomassie Blue R-250. Mr, Bio-Rad Precision Plus dual color markers (5 ÎĽl).