Muscle free amino acid profiles are related to differences in skeletal muscle growth between single and twin ovine fetuses near term

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Table 1 Free amino acid concentration in semitendinosus muscle: three-way interaction

	Heavy ewes				Light ewes				LSD	P RxSxN
	Ad libitum		Maintenance		Ad libitum		Maintenance
	Single	Twin	Single	Twin	Single	Twin	Single	Twin
Essential amino acids
L-Histidine	225	242	201	232	199	261	227	259	76	0.57
L-Leucine	60	52	54	40	50	42	60	47	15	0.96
L-Lysine	109	115	80	81	67	88	70	119	37	0.37
L-Methionine	48	79	42	64	46	86	46	74	17	0.85
L-Phenylalanine	41	50	47	37	44	43	48	47	13	0.18
L-Threonine	1252	823	968	841	1222	839	1212	1065	287	0.82
L-Valine	175	98	119	69	150	121	191	119	38	0.07
Total EAA	1,690	1,423	1,455	1,272	1,763	1,462	1,707	1,713	185	0.55
Non essential amino acids
L-Alanine	2393	2495	2239	1858	2137	2164	2400	2164	368	0.55
L-Arginine¹	410	239	424	263	392	263	340	333	88	0.20
L-Aspartic Acid	465	302	132	85	500	343	423	345	96	0.70
L-Carnosine	1407	1379	1297	1124	1157	1432	1148	1088	323	0.56
L-Citrulline	75	53	59	52	87	72	92	97	27	0.86
L-Cystathionine	140	97	218	157	153	169	287	208	102	0.45
L-Glutamic acid	1483	1240	1191	1201	1045	1807	1365	1835	369	0.14
L-Glutamine	3202^a	3276^a	3111^a	1973^b	3302^a	3539^a	3043^a	3514^a	609	0.02
L-Glycine	1844	2794	2010	2868	1888	2215	2134	2052	543	0.56
L-Ornithine	102	99	93	119	69	92	90	113	29	0.32
L-Proline	352	246	370	295	319	285	322	391	105	0.51
L-Serine	1353	1520	995	1474	772	1365	1239	1452	382	0.07
L-Taurine	5501	6046	5786	5770	6170	6728	6485	6939	1348	0.74
L-Tyrosine	51^a	57^a	60^a	52^b	64^a	44^a	68^a	72^a	17	0.03
Total NEAA	18,634	19,640	17,665	17,238	17,766	19,831	19,361	20,227	895	0.90
TOTAL	20,359	21,118	19,177	18,590	19,567	21,358	21,114	22,000	942	0.82

¹Deemed as conditionally essential (Wu 2009).
Three-way interaction between maternal size,(S, Heavy vs. Light) plane of nutrition (N, Ad libitum vs. Maintenance) and pregnancy rank (R, Single vs. Twin) for the concentration (nmol/g wet tissue) of free amino acids in M. semitendinosus of fetuses at 140 days gestation. Values are expressed as least square mean (LSM). The average of the difference of the least square means (LSD, α = 0.05) and probability of significance for the three way interaction (RxSxN) are presented.