Genetic diversity and relationship of cattle populations of East India: distinguishing lesser known cattle populations and established breeds based on STR markers

Sharma, Rekha; Maitra, Avishek; Singh, Pramod Kumar; Tantia, Madhu Sudan

doi:10.1186/2193-1801-2-359

SpringerPlus

Table 1 Characteristics of 21 microsatellite loci used in present study

From: Genetic diversity and relationship of cattle populations of East India: distinguishing lesser known cattle populations and established breeds based on STR markers

Primers	Primer sequences (5′-3′)	Forward label	Set	Annealing temp	Product size (bp)	Total number of alleles per locus
BM1824	gagcaaggtgtttttccaatc	VIC	4	58°C	178-194	7
BM1824	cattctccaactgcttccttg	VIC	4	58°C	178-194	7
CSSM08	cttggtgttactagccctggg	VIC	3	55°C	182-200	7
CSSM08	gatatatttgccagagattctgca	VIC	3	55°C	182-200	7
CSSM33	cactgtgaatgcatgtgtgtgagc	NED	5	58°C	148-186	19
CSSM33	cccatgataagagtgcagatgact	NED	5	58°C	148-186	19
CSSM66	acacaaatcctttctgccagctga	FAM	4	60°C	167-207	17
CSSM66	aatttaatgcactgaggagcttgg	FAM	4	60°C	167-207	17
ETH10	gttcaggactggccctgctaaca	NED	1	58°C	185-221	14
ETH10	cctccagcccactttctcttctc	NED	1	58°C	185-221	14
ETH225	gaacctgcctctcctgcattgg	VIC	4	64°C	134-156	11
ETH225	actctgcctgtggccaagtagg	VIC	4	64°C	134-156	11
ETH3	gatcaccttgccactatttcct	NED	4	57°C	92-122	14
ETH3	acatgacagccagctgctact	NED	4	57°C	92-122	14
HEL09	cccattcagtcttcagaggt	FAM	5	59°C	140-168	13
HEL09	cacatccatgttctcaccac	FAM	5	59°C	140-168	13
HEL5	gcaggatcacttgttaggga	VIC	3	55°C	137-187	20
HEL5	agacgttagtgtacattaac	VIC	3	55°C	137-187	20
ILSTS06	tgtctgtatttctgctgtgg	FAM	5	58°C	279-303	11
ILSTS06	acacggaagcgatctaaacg	FAM	5	58°C	279-303	11
ILSTS11	gcttgctacatggaaagtgc	NED	1	58°C	261-269	5
ILSTS11	ctaaaatgcagagccctacc	NED	1	58°C	261-269	5
ILSTS34	aagggtctaagtccactggc	VIC	5	59°C	138-208	29
ILSTS34	gacctggtttagcagagagc	VIC	5	59°C	138-208	29
ILSTS33	tattagagtggctcagtgcc	PET	3	55°C	137-163	10
ILSTS33	atgcagacagttttagaggg	PET	3	55°C	137-163	10
INRA05	caatctgcatgaagtataaatat	FAM	2	54°C	130-144	8
INRA05	cttcaggcataccctacacc	FAM	2	54°C	130-144	8
INRA35	atcctttgcagcctccacattg	FAM	3	54°C	80-142	24
INRA35	ttgtgctttatgacactatccg	FAM	3	54°C	80-142	24
INRA63	atttgcacaagctaaatctaacc	PET	2	54°C	164-188	11
INRA63	aaaccacagaaatgcttggaag	PET	2	54°C	164-188	11
MM12	caagacaggtgtttcaatct	PET	4	52°C	88-132	20
MM12	atcgactctggggatgatgt	PET	4	52°C	88-132	20
MM8	cccaaggacagaaaagact	NED	2	55°C	114-140	10
MM8	ctcaagataagaccacacc	NED	2	55°C	114-140	10
TGLA122	ccctcctccaggtaaatcagc	VIC	1	58°C	135-179	18
TGLA122	aatcacatggcaaataagtacatac	VIC	1	58°C	135-179	18
TGLA227	cgaattccaaatctgttaatttgct	PET	2	55°C	97-119	16
TGLA227	acagacagaaactcaatgaaagca	PET	2	55°C	97-119	16
TGLA53	gctttcagaaatagtttgcattca	FAM	1	58°C	142-184	20
	atcttcacatgatattacagcaga

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