Marinobacter hydrocarbonoclasticus NY-4, a novel denitrifying, moderately halophilic marine bacterium
© Li et al.; licensee Springer. 2013
Received: 13 April 2013
Accepted: 26 July 2013
Published: 27 July 2013
The isolation and characterization of a novel halophilic denitrifying marine bacterium is described. The halophilic bacterium, designated as NY-4, was isolated from soil in Yancheng City, China, and identified as Marinobacter hydrocarbonoclasticus by 16S rRNA gene sequence phylogenetic analysis. This organism can grow in NaCl concentrations ranging from 20 to 120 g/L. Optimum growth occurs at 80 g/L NaCl and pH 8.0. The organism can grow on a broad range of carbon sources and demonstrated efficient denitrifying ability (94.2% of nitrate removal and 80.9% of total nitrogen removal in 48 h). During denitrification by NY-4, no NO2 --N was accumulated, N2 was the only gaseous product and no harmful N2O was produced. Because of its rapid denitrification ability, broad carbon use range and ability to grow under high salinity and pH conditions, NY-4 holds promise for the treatment of saline waste waters.
Excessive nitrate contamination in surface and ground water leads to many health and other problems, so that nitrogen (N) removal from water is of great importance (Paerl et al., 2002; Spalding and Exner, 1993). Denitrification is a bacterial process that can remove nitrogen from wastewater through the heterotrophic conversion of nitrate to N2 (Khardnavis et al., 2007). This biological process is highly efficient at nitrogen removal, and does not produce any secondary pollution or residues (Song et al., 2011).
Denitrification occurs in many species of the bacteria and archaea (Zumft 1992), such as Pseudomonas stutzeri (Su et al., 2001), Paracoccus denitrificans (Robertson and Kuenen, 1983), Alcaligenes faecalis (Joo et al., 2005), Bacillus spp. (Kim et al., 2005), and Microvirgula aerodenitrificans (Patureau et al., 2001). Most denitrifying organisms used for biotechnological applications are mesophilic denitrifying bacteria (pH optima near 7.0 and with low or no salt tolerance). These strains are not always suitable for nitrate removal at high salt concentrations and high pH of industrial wastewaters (van der Hoek et al., 1987). Unlike domestic or landscape wastewaters, industrial wastewaters are complex matrices, which may contain many cell growth inhibitors (Hockenbury and Grady, 1977; Grunditz et al., 1998), such as high salt concentrations. As previously reported, when the salt concentration was higher than 6 g/L, a significant drop in the denitrification efficiency was observed, because many bacteria died when the salt concentration was higher than 6 g/L (Vendramel et al., 2011).
Use of bacterial strains that have both efficient denitrifying ability and salt tolerance in industrial wastewater treatment may solve this problem. As reported, denitrification under moderately halophilic conditions (pH range from 7.0 to 9.0 and a salt concentration under 2M Na+) has been shown for several members of the Gammaproteobacteria, such as Thioalkalivibrio spp. (Sorokin et al., 2001; Sorokin and Kuenen 2005) and Halomonas spp. (Berendes et al., 1996; Mormille et al., 1999; Peyton et al., 2001; Romano et al., 2005; Boltyyansakaya et al., 2004, Boltyanskaya et al. 2007). Some Halomonas strains were also demonstrated to actively denitrify under highly halophilic conditions (pH 9.0 and a salt concentration of 4M Na+) (Klup et al., 2007; Shapovalova et al., 2008). It is unfortunate that all these reported halophilic species only grew and actively denitrified under aerobic conditions. Because of the low concentrations of dissolved oxygen in industrial wastewaters, these reported aerobic halophilic species were not suitable for application in treatment of industrial wastewater (Shapovalova et al., 2008).
The objective of this research was to isolate and characterize new halophilic denitrifying bacteria with better potential for saline industrial wastewater treatment. To achieve this objective, sediment samples were collected from Xintan Saltern, Yancheng City, Jiangsu Province, China. A halophilic bacterium NY-4 was isolated and identified as Marinobacter sp. by 16S rRNA analysis. NY-4 has a high anaerobic denitrifying efficiency (94.2% nitrite removal and 80.9% total nitrogen removal in 48 h), moderate salt tolerance (from 20 g/L to 120 g/L), pH tolerance (from 7.0 to 9.0) and broad carbon use range, which makes NY-4 a promising inoculant for saline industrial wastewater treatment.
Materials and methods
Strains, medium and culture condition
NY-4 was isolated from soil in Xintan Saltern, Yancheng City, Jiangsu Province, China, and maintained in 8% glycerol HSLB plus medium (High Salinity LB medium, LB with 80 g/L NaCl) at −80°C. For the isolation of bacterial strains, a soil sample was suspended and vortexed thoroughly in sterile H2O and serially diluted 10-fold four times using sterile H2O. One hundred μl of the 10-4 dilution was spread on an HSLB plus plate and incubated at 30°C for 48 h. Single colonies were selected. NY-4 was obtained after strict investigation of its purity.
The following indicates the contents of each medium that was prepared: Denitrification medium (DM, g/L): NaNO3, 0.607; KH2PO4, 5; NaCl, 80, trace element solution, 1 ml, pH, 7.0-8.0; the amount of carbon source varied by experiments; trace element solution (g/L): Na2EDTA, 63.7; ZnSO4, 2.2; CaCl2, 5.5; MnCl2 .4H2O, 5.06; FeSO4.7H2O, 5.0; Na2MoO4 .4H2O, 1.1; CuSO4 .5H2O, 1.57; CoCl2 .6H2O, 1.61, pH, 7.0-7.5. High Salinity LB plus medium (HSLB, g/L): tryptone, 10; yeast extract, 5; NaCl, 80; KCl, 5; MgSO4 .7H2O, 2.5; pH, 7.0-7.5. To prepare HSLB plus medium plates, 2% (w/v) agar was added. For strain maintenance, glycerol was added into LB plus liquid medium to a final concentration of 8% (v/v).
NY-4 was transferred from an HSLB plus frozen culture maintained at −80°C to a fresh HSLB plus medium plate and incubated at 30°C for 24 h. NY-4 from the plate was inoculated into the HSLB plus liquid media and incubated at 30°C at 150 rpm for 18 h (about OD600 at 5.0) as the preculture. The preculture was inoculated into DM medium and cultivated as described for each experiment.
Electron microscopy observation
Detailed cell shapes and flagella were observed both by scanning electron microscopy and transmission electron microscopy. For scanning electron microscopy analysis, bacteria were fixed with 1% glutaraldehyde overnight and then dehydrated in ethanol completely. Cells were coated with gold-palladium and observed with a JSM-5610LV scanning electron microscope (JEOL Ltd., Tokyo, Japan) (Bertrand et al., 1990). For transmission electron microscopy examinations, cells were negatively stained with phosphotungstic acid according to the method of Jahn (1986) and observed with an H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
16S rRNA analysis
The 16S rRNA was amplified by PCR using the universal bacterial primers 27F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-GGYTACCTTGTTACGACTT-3’) (Lane 1991) and was sequenced by TAKARA Corp. (Dalian, China). The sequence was submitted to a Blast search to compare with available 16S rRNA gene sequences in GenBank of the NCBI database (Altschul et al., 1990). A neighbor-joining tree was constructed using the MEGA 4.0 program (Kumar 2004).
A 1.5 mL of preculture of NY-4 in HSLB plus medium was inoculated in 150 mL of DM liquid medium sealed with 15 mL paraffin wax, contained in 250-mL flasks. The flasks were then incubated at 30°C in a constant temperature incubator. The cultures were sampled periodically to determine bacterial growth and the concentration of NO2 --N, NO3 --N and the total nitrogen (TN) were measured every 12 h during incubation for 72 h. The same denitrification process was performed to detect gaseous nitrogen products, with the exception that tightly sealed infusion bottles (150 mL DM/250-mL flask) were used. N2 or N2O product was monitored in DM medium at the end of cultivation (72 h).
Effect of culture conditions on denitrification performance of NY-4
The effects of the NaCl concentration, carbon source, C/N ratio, and initial pH on the denitrification performance of NY-4 were investigated by single factor tests (Tang and Luo, 2008).
In experiments to determine the role of the carbon source, sodium succinate, trisodium citrate, sodium acetate and ethanol were used as the sole carbon source in DM. The other conditions were as follows: initial concentration, 100 mg/L of NO3 --N; C/N = 10; 80 g/L of NaCl; initial pH of 8.0; and temperature of 30°C. In the experiments to test the effect of C/N ratio, trisodium citrate served as the sole carbon source, and the concentration was varied to yield different C/N ratios (1, 5, 10, 15, 20, 25 and 30) at a fixed concentration of NO3 --N (100 mg/L). The other conditions were the same as those used for the carbon source experiments. To observe the effects of salinity and initial pH on nitrogen removal, the concentration of NaCl in DM was varied from 0 to 160 g/L (at intervals of 20 g/L), and the initial pH from 6.0 to 10.0 (at intervals of 1.0 pH units). These tests were conducted at a C/N ratio that demonstrated optimum growth conditions. Samples were collected for the determination of bacterial growth rate, NO2 --N, NO3 --N and TN during incubation for 48 h.
Bacterial cells were stained with a Gram staining method (Magee et al., 1975), observed and counted in blood counting chamber, using an optical microscope (LW40B, Cewei Corp. Ltd., China). Growth rate was calculated as change in the number of incerased cells vs culturing time during the exponential phase of growth.
Nitrate (NO3 --N), nitrite (NO2 --N), and total nitrogen (TN) were determined according to standard methods (APHA et al., 1998), including diphenylamine spectrophotometry, N-(1-naphthyl)-1, 2-diaminoethane dihydrochloride spectrophotometry, and potassium persulfate digestion UV spectrophotometric methods, respectively.
The gaseous nitrogen products N2O and N2 from headspace were analyzed by gas chromatography (GC9790-2, Guji Corp. Ltd., China). The conditions were as follows: Packed GC Column [Porapak Q] (mesh size 60/80, Φ3×3 m, Agilent); sample injection port and detector temperature set at 70°C and 100°C, respectively. Gas flow: carrier H2 1.2 mL/min; the added quantity was 600 μL.
Amplification of the nitrite reductase gene and nitrous oxide reductase of NY-4
Fragments of the nir S gene of NY-4 were amplified using the primers cd3F and cd4R, developed by Michotey et al. (2000). The primer sequences were cd3F: 5’- GT(A/T/C/G)AA(T/C)GT(A/T/C/G)AA(A/G)GA(A/G)AC(A/T/C/G)GG-3’, cd4R: 5’-AC(A/G)TT(A/G)AA(T/C)TT(A/T/C/G)CC(A/T/C/G)GT(A/T/C/G)GG-3’. Fragments of the nir K gene of NY-4 were amplified using primers F1aCu and R3Cu, developed by Hallin et al. (1999). The primer sequences were F1aCu: 5’-ATCATGGT(C/G)CTGCCGCG-3’, R3Cu: 5’-GCCTCGATCAG(A/G)TTGTGGTT-3’. Fragments of the nos Z of NY-4 were amplified using primers nosz47F and nosz1776R, referenced with the reported potential nos Z gene (GenBank, GI: 107515115). The primer sequences were nosz47F: 5’-GAGGTTTCCGAAGGTGGTCTG-3’, nosz1776R: 5’-GGTCACGGAAGCGGTCTGC-3’. The PCR conditions used were according to the references described for each primer pair. Aliquots of 10 μl of the reactions were analyzed by electrophoresis on 2% (w/v) agarose gels. Bands were visualized by UV excitation and sequenced by TAKARA Corp. (Dalian, China). The sequences were compared with available reported gene sequences in GenBank using the BLAST program.
All results were shown as the average of at least three independent experiments; variation was expressed as SD. Student’s t-test, analysis of variance (ANOVA) and the Duncan’s multiple range tests were used to determine whether the nitrite-N degradation rate differed significantly between treatments (P < 0.05). All statistics were performed using SPSS for Windows version 11.0.
Identification of NY-4
Factors affecting denitrification of NY-4
We tested the effects of the NaCl concentration, carbon source, C/N ratio, and initial pH on the denitrification performance of NY-4 by single factor tests (Tang and Luo, 2008). In most different cultivation conditions, NY-4 had grown at a maximum cell density and entered the stationary phase when the cultures were sampled at 48 h, except for no growth in extreme unsuitable conditions. In addition, cells of NY-4 at the similar time is in the same growth phase so that the data of nitrogen removal were significative and comparable with each other.
As the main aim in this study was to isolate and identify strains with both effective denitrifying ability and high tolerance to salinity, we first assessed the denitrifying ability of NY-4 cultured in DM medium with a large range of salt concentrations, from 0 to 160 g/L NaCl.
The carbon source is one of the most important factors affecting denitrifying ability. During the denitrification process, cells use the carbon source as an electron donor and gradually reduce nitrate to N2, thus removing organic matter and nitrate simultaneously. Her and Huang (1995) proposed that the structure and molecular content of the carbon source were significant factors affecting the efficiency of denitrification.
Effect of carbon source on the denitrifying performance of NY-4
Growth rate (106mL-1/h)
NO3 --N removal (% 48 h)
TN removal (% 48 h)
1.29 ± 0.13
88.8 ± 2.1
71.0 ± 3.2
1.38 ± 0.18
92.9 ± 3.5
83.1 ± 2.4
0.68 ± 0.09
52.1 ± 3.3
43.9 ± 4.1
0.10 ± 0.01
8.4 ± 1.6
6.8 ± 1.2
Generally speaking, a carbon source with a simple, small molecular structure would be more favorable for denitrification. For NY-4, sodium succinate was the best carbon source, probably because sodium succinate can easily enter the tricarboxylic acid cycle (TCA) and rapidly provide energy and reduction force (Zheng et al., 2012).
Interestingly, even with all the different factors we changed and assessed, no obvious NO2 --N accumulation was detected, which meant that any NO2 --N produced was quickly used as an electron donor and converted to N2O by NY-4.
Detection of gaseous products of NY-4
At the end of the incubation of NY-4, lots of bubbles were observed in the cell cultures. Because no NO2 --N accumulation was detected, we presumed that any NO2 --N formed by denitrification was quickly converted to gaseous products. The gaseous nitrogen products of denitrification are N2O or N2 (Jones et al., 2011), which has been demonstrated in some species of bacteria such as Alcaligenes faecalis (Joo et al., 2007). Nitrous oxide and N2 from the culture headspace were analyzed by gas chromatography. As shown in Additional file 2: Figure S2, the peak of a standard sample of N2 emerged between 0.664 min and 1.013 min, while the peak of a standard sample of N2O emerged between 2.063 min and 3.026 min. The peak of gaseous nitrogen production from NY-4 appeared between 0.673 min to 1.002 min, corresponding to the standard N2 peak. A small peak was detected at 2.095 min, corresponding to N2O. This result demonstrated that NY-4 could produce both N2O and N2 through its denitrification process, but N2O was rapidly converted to N2 so that negligible N2O was detected (Additional file 2: Figure S2).
Amplification of denitrification nitrite reductase and nitrous oxide reductase of NY-4
Denitrification is part of the bioenergetic ability of the bacterial cell, where the nitrate and nitrite and the gaseous NO and N2O serve instead of dioxygen (O2) as terminal acceptors for electron transport phosphorylation. It is clearly the role of denitrification in the global N cycle and in cellular bioenergetics that makes a detailed knowledge of this process essential (Zumfit 1997).
Denitrification will be considered as the assemblage of nitrate respiration, nitrite respiration combined with NO reduction, and N2O respiration:
NO3 -→NO2 -→NO→N2O→N2
In the denitrifying process, three gases (NO, N2O and N2) can be produced. Because of the high activity and specificity of NO reductase, NO is hardly accumulated, so the main gaseous products of the denitrifying process are N2O and N2. N2O is an important greenhouse gas, with ~320-fold higher impact than CO2 on greenhouse effect (Lashof and Ahuja, 1990). For this reason it was important to isolate strains that completely denitrify NO3 --N to N2. The presence of the nosZ gene, encoding nitrous oxide reductase, indicates whether the strain has the genetic capacity for complete reduction to N2 (Jones et al., 2011).
We analyzed the gases produced by NY-4 and found that the majority of gas produced was N2 with very little N2O accumulation. We were able to amplify the nos Z gene of NY-4 (1896 nt) (Figure 6). The BLAST result showed this nos Z gene shared 94% homology with a reported M. hydrocarbonoclasticus potential nos Z gene (GenBank, GI: 107515115). We postulate that the nitrous oxide reductase encoded by the nosZ gene of NY-4 had a very high enzymatic activity so that the N2O produced was quickly converted to N2.
As we know, industrial wastewaters contain a large amount of nitrates and salt (Glass and Silverstein, 1997; Hirata et al., 2001; Labbe et al., 2003). van der Hoek et al. (1987) and Clifford and Liu (1993) showed that denitrification was possible under 3% (w/w) NaCl conditions. However, the denitrification rate of saline wastewaters decreased under highly saline conditions (Yang et al., 1995). Therefore, nitrogen removal from saline wastewater has been considered to be difficult (Yoshie et al., 2004). The discovery of denitrifying halophilic bacteria was thought to solve this problem and many such strains have been reported. Most of these strains only grow under aerobic conditions, which limits their use in treatment of low DO industrial wastewaters.
In this study, a novel M. hydrocarbonoclasticus NY-4 was shown to almost completely reduce nitrate under optimum conditions (80 g/L NaCl and pH 8.0). Compared with other halophilic species, NY-4 could grow faster (maximum at 1.39 × 106 mL-1/h) and to a higher density (3.92 × 108 mL-1) under anaerobic conditions. M. hydrocarbonoclasticus NY-4 also rapidly converted NO3 --N into N2, without NO2 --N or N2O accumulation. Generally, NY-4 could reduce nitrate within a NaCl concentration range from 20 to 120 g/L and pH range from 7.0 to 9.0. The strain was also able to use a range of carbon sources for growth and denitrification, such as sodium succinate, trisodium citrate and sodium acetate. Because of its denitrification ability, broad carbon use range and high tolerance to salinity and pH, NY-4 holds promise for the treatment of saline wastewaters.
We also tested the denitrifying ability of NY-4 under aerobic conditions (data not shown). The best TN removal was only about 40% under aerobic conditions, which is no better than other reported strains in nitrogen removal. Considering the low dissolved oxygen in industrial wastewaters, NY-4 remains a promising potential inoculant for saline wastewater treatment.
LRP and HN carried out the strains isolation, characteristics, analysis of denitrification ability, and drafted the manuscript. ZXL carried out the 16S rRNA gene amplication. WXF carried out researches on effect of culture conditions on cell growth. ZX and GHF participated in the cell cultivations and 16S rRNA sequence alignment. All authors read and approved the final manuscript.
This research was supported by the National Science Foundation of China (No. 51008012) and Open Science Foundation of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology (No. JSBEET1314).
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J. Mol. Biol 1990, 215: 403-140.View ArticleGoogle Scholar
- APHA, AWWA, and WPCF: Standards methods for the examination of water and wastewater. 9th edition. APHA, Washington, D.C; 1998.Google Scholar
- Berendes F, Gottschalk G, Heine-Donnernack E, Moore ERB, Tindall BJ: Halomonas desiderata sp. Nov., a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. Syst. Appl. Microbiol 1996, 19: 158-167. 10.1016/S0723-2020(96)80041-5View ArticleGoogle Scholar
- Bertrand JC, Al-Mallah M, Acquaviva M, Mille G: Biodegradation of hydrocarbons by an extremely halophilic archaebacterium. Lett. Appl. Microbiol 1990, 11: 260-263. 10.1111/j.1472-765X.1990.tb00176.xView ArticleGoogle Scholar
- Boltyanskaya YV, Antipov AN, Kolganova TV, Lysenko AM, Kostrikina NA, Zhilina TN: Halomonas campisalis , an obligatorily alkaliphilic, nitrous oxide-reducing denitrifier with a molybdenum cofactor-lacking nitrate reductase. Mikrobiologia (Moscow Engl Transl) 2004, 73: 271-278.View ArticleGoogle Scholar
- Boltyanskaya YV, Kevbrin VV, Lysenko AM, Kolganova TV, Tourova TP, Osipov GA, Zhilina TN: Halomonas mongoliensis sp. nov. and Halomonas kenyensis sp. nov., new haloalkaliphilic denitrifiers capable of N2O reduction, isolated form soda lakes. Mikrobiologia (Moscow Engl Transl) 2007, 76: 739-747.View ArticleGoogle Scholar
- Brake G, Zhou J, Wu L, Shou JZ, Allan H: Nitrite reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in pacific northwest marine sediment communities. Appl. Environ. Microbool 2000, 66: 2096-2104. 10.1128/AEM.66.5.2096-2104.2000View ArticleGoogle Scholar
- Cliford D, Liu X: Ion exchange for nitrate removal. J. Am. Water. Works. Assoc 1993, 85: 135-143.Google Scholar
- Glass C, Silverstein J: Denitrification of high-nitrate high-salinity, wastewater. Water. Res 1997, 33: 223-229.View ArticleGoogle Scholar
- Grunditz C, Gumaelius L, Dalhammar G: Comparison of inhibition assays using nitrogen removing bacteria: application to industrial wastewater. Water. Res 1998, 32: 2995-3000. 10.1016/S0043-1354(98)00050-5View ArticleGoogle Scholar
- Hallin S, Lindgren P: PCR detection of genes encoding nitrite reductase in denitrifying bacteria. Appl. Environ. Microbiol 1999, 65: 1652-1657.Google Scholar
- Her JJ, Huang JS: Influences of carbon source and C/N ratio on nitrate/nitrate denitrification and carbon breakthrough. Bioresour. Technol 1995, 54: 45-51. 10.1016/0960-8524(95)00113-1View ArticleGoogle Scholar
- Hirata A, Nakamura Y, Tsuneda S: Nitrogen removal from industrial wastewater discharged from metal recovery processes. Water. Sci. Technol 2001, 44: 171-180.Google Scholar
- Hockenbury MR, Grady CPL Jr: Inhibition of nitrification effects of selected organic compounds. J. Water. Pollut. Control. Fed 1977, 49: 768-777.Google Scholar
- Jahn K: Isolation and characterization of fimbriae form Wscherichia coli. In The virulence of Escherichia coli. Reviews and methods. The Society for General Microbiology. Edited by: Sussman M. Academic Press, London; 1986:381-388.Google Scholar
- Jones CM, Weish A, Throback IN, Dorsch P, Bakken LR, Hallin S: Phenotypic and genotypic heterogeneity among closely related soil-borne N2- and N2O- producing Bacillus isolates harboring the nosZ gene. FEMS Micobiol Ecol 2011, 76: 541-552. 10.1111/j.1574-6941.2011.01071.xView ArticleGoogle Scholar
- Joo HS, Hirai M, Shoda M: Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis no. 4. J. Biosci. Bioeng 2005, 100: 184-191. 10.1263/jbb.100.184View ArticleGoogle Scholar
- Joo HS, Hirai M, Shoda M: Improvement in ammonium removal efficiency in wastewater treatment by mixed culture of alcaligenes faecalis no. 4 and L1. J. Biosci. Bioeng 2007, 103: 66-73. 10.1263/jbb.103.66View ArticleGoogle Scholar
- Khardnavis AA, kapley A, Purohit KJ: Simultaneous nitrification and denitrification by diverse Diaphorobacter sp. Appl. Microbiol. Biotechnol 2007, 77: 403-409. 10.1007/s00253-007-1176-5View ArticleGoogle Scholar
- Kim JK, Paik KJ, Cho KS, Nam S-W, Park TJ, Bajpai R: Aerobic nitrification-denitrification by heterotrophic Bacilis strains. Bioresour. Technol 2005, 96: 1897-1906. 10.1016/j.biortech.2005.01.040View ArticleGoogle Scholar
- Kulp TR, Han S, Saltikov CW, Lanoil BD, Zargar K, Oremland RS: Effects of imposed salinity gradients on dissimilatory arsenate reduction, sulfate reduction, and other microbial processes in sediments from two California soda lakes. Appl. Environ. Microbiol 2007, 73: 5130-5137. 10.1128/AEM.00771-07View ArticleGoogle Scholar
- Kumar S, Tamura K, Nei M: MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform 2004, 5: 150-163. 10.1093/bib/5.2.150View ArticleGoogle Scholar
- Labbe N, Juteau P, Parent S, Villemur R: Bacterial diversity in a marine methanol-fed denitrification reactor at the Montreal bidome. Canada. Microb. Ecol 2003, 46: 12-21. 10.1007/s00248-002-1056-6View ArticleGoogle Scholar
- Lane DJ: 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systematics. Edited by: Stackebrandt E, Goodfellow M. Wiley, Chichester; 1991:115-175.Google Scholar
- Lashof DA, Ahuja DR: Relative contributions of greenhouse gas emissions to global warming. Nature 1990, 344: 529-531. 10.1038/344529a0View ArticleGoogle Scholar
- Lesley A, Robortson LA, Kuenen JG: Thiosphaera pantotropha gen. nov., sp. Nov., a facultative autotrophic sulphur bacterium. J. Gen. Microbiol 1983, 129: 2847-2855.Google Scholar
- Magee CM, Rodeheaver G, Edgerton MT, Edlich RF: A more reliable Gram staining technique for diagnosis of surgical infections. Am. J. Surg 1975, 130: 341-346. 10.1016/0002-9610(75)90398-0View ArticleGoogle Scholar
- Michotey V, Mejean V, Bonin P: Comparison of methods for quantification of cytochrome cd1-denitrification bacteria in environmental marine sample. Appl. Environ. Microbiol 2000, 66: 1564-1571. 10.1128/AEM.66.4.1564-1571.2000View ArticleGoogle Scholar
- Mormile MR, Romine MF, Garcia MT, Ventosa A, Bailey TJ, Peyton BM: Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst. Appl. Microbiol 1999, 22: 551-558. 10.1016/S0723-2020(99)80008-3View ArticleGoogle Scholar
- Ole F, Maurizio B, Francesca C, Serena R, Scot W, Israel P: Intramolecular electron transfer in Pseudomonas aeruginosa cd1 nitrite reductase: thermodynamics and kinetics. Biophys. J 2009, 96: 2849-2856. 10.1016/j.bpj.2008.12.3937View ArticleGoogle Scholar
- Paerl HW, Dennis RL, Whitall DR: Atmospheric deposition of nitrogen: implications for nutrient over-enrichment of coastal waters. Estuaries 2002, 25: 677-693. 10.1007/BF02804899View ArticleGoogle Scholar
- Patureau D, Helloin E, Rustrain E, Bouchez T, Delgenes JP, Moletta R: Combined phosphate and nitrogen removal in a sequencing batch reactor using the aerobic denitrifier., Microvirgula aerodenitrificans . Water. Res 2001, 35: 189-197. 10.1016/S0043-1354(00)00244-XView ArticleGoogle Scholar
- Peyton BM, Mormile MR, Petersen JN: Nitrate reduction with Halomonas campsisalis : kinetics of denitrification at pH 9 and 12.5% NaCl. Water. Res 2001, 35: 4237-4242. 10.1016/S0043-1354(01)00149-XView ArticleGoogle Scholar
- Robertson LA, Kuenen JG: Thiosphaera pamtotropha gen. nov., sp. Nov., a facultatively anaerobic, facultatively antotrophic sulphur bacterium. J. Gen. Microbiol 1983, 129: 2847-2855.Google Scholar
- Romano I, Giordano A, Lama L, Nicolaus B, Gambacorta A: Halomonas campaniensis sp. nov., a haloakaliphilic bacterium isolated from a mineral pool of Campania region. Italy. Syst. Appl. Microbiol 2005, 28: 610-618. 10.1016/j.syapm.2005.03.010View ArticleGoogle Scholar
- Shao Q, Yu XB: Isolation and characterization of a strain denitrifier. J. Biotechnol 2008, 18: 63-65.Google Scholar
- Shapovalova AA, Khijniak TV, Tourova TP, Muyzer G, Sorokin DY: Heterotrophic denitrification at extremely high salt and pH by haloalkaliphilic Gammaproteobacteria from hypersaline soda lakes. Extremophiles 2008, 12: 619-625. 10.1007/s00792-008-0166-6View ArticleGoogle Scholar
- Song ZF, An J, Fu GH, Yang XL: Isolation and characterization of an aerobic denitrifying bacillus sp. YX-6 form shrimp culture ponds. Aquaculture 2011, 319: 188-193. 10.1016/j.aquaculture.2011.06.018View ArticleGoogle Scholar
- Sorokin DY, Kuenen JG: Alkaliphilic chemolithotrophs from sodas lakes. FEMS. Microbiol. Ecol 2005, 52: 287-295. 10.1016/j.femsec.2005.02.012View ArticleGoogle Scholar
- Sorokin DY, Kuenen JG, Jetten M: Denitrification at extremely alkaline conditions in obligately autotrophic alkaliphilic sulfuroxidizing bacterium Thioalkalivibrio denitrificans . Arch. Microbiol 2001, 175: 94-101. 10.1007/s002030000210View ArticleGoogle Scholar
- Spalding RF, Exner ME: Occurrence of nitrate in groundwater – a review. J. Environ. Qual 1993, 22: 392-402.View ArticleGoogle Scholar
- Stouthamer AH: Metabolic pathways in Paracoccus denitrificans and closely related bacterial in relation to the phylogeny of prokaryotes. Anton. Leeuw. Int. J.C 1992, 61: 1-33. 10.1007/BF00572119View ArticleGoogle Scholar
- Su JJ, Liu BY, Liu CY: Comparison of aerobic denitrification under high oxygen atmosphere by Thiosphaera pantotropha ATCC 35512 and Pseudomonas stutzeri SU2 newly isolated form the activated sludge of a piggery wastewater treatment system. J. Appl. Microbiol 2001, 90: 457-462. 10.1046/j.1365-2672.2001.01265.xView ArticleGoogle Scholar
- Tang L, Luo GY: Study on nitrogen removal characteristics of an aerobic denitrifying bacterium Rhodococcus strain T7. Environ. Ecol. Three Gorges 2008, 1: 24-27.Google Scholar
- Van der Hoek JP, Latour PJM, Klapwijk A: Denitrification with methanol in the presence of high salt concentrations and at high pH levels. Appl. Microbiol. Biotechnol 1987, 27: 199-205.View ArticleGoogle Scholar
- Vendramel S, Dezotti M, Sant’Anna GL: Nitrification of an industrial wastewater in a moving-bed biofilm reactor: effect of salt concentration. Environmental. Technology 2011, 32: 837-846. 10.1080/09593330.2010.514949View ArticleGoogle Scholar
- Yang PY, Nitisoravut S, Wu JS: Nitrate removal using mixted-culture entrapped microbial cell immobilization process under high salt condition. Water. Res 1995, 29: 1525-1532. 10.1016/0043-1354(94)00296-JView ArticleGoogle Scholar
- Yoshie S, Noda N, Tsuneda S, Hirata A, Inamori Y: Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system. FEMS Lett 2004, 235: 183-189. 10.1111/j.1574-6968.2004.tb09585.xView ArticleGoogle Scholar
- Zheng HY, Liu Y, Gao XY, Ai GM, Miao LL, Liu ZP: Characterization of a marine origin aerobic nitrifying-denitrifying bacterium. J. Biosci. Bioeng 2012, 114: 33-37. 10.1016/j.jbiosc.2012.02.025View ArticleGoogle Scholar
- Zumft WG: The denitrifying prokaryotes. In The Prokaryotes. 2nd edition. Edited by: Balows A, Truper HG, Dworkin M, Harder W, Schlerfer K-H. Springer-Verlag, New York; 1992:554-582.Google Scholar
- Zumft WG: Cell biology and molecular basis of denitrification. Microb. Mol. Biol. Rev 1997, 61: 533-616.Google Scholar
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