Fig. 2

Fluorescent micrographs of AO/PI double stained of HepG2 cells treated with C. betacea at 30 µg/ml for 24, 48 and 72 h. a Untreated HepG2 cells showing normal cell structure and viable cells fluoresce green with round intact nuclei. b After 24 h treated group showing chromatin condensation, membrane blebbing and late apoptosis. c Chromatin condensation, membrane blebbing, DNA fragmentation and late apoptosis events were observed after 48 h of treatment. d Apoptotic body formation after 72 h of treatment. e HepG2 cell treated with 8 µg/ml of doxorubicin showed early apoptosis event and formation of apoptotic body. Scale bars (A–E): 200 μm (magnification: × 200). VC Viable cells, BL Blebbing of cell membrane, CC Chromatin condensation, FN Fragmented nuclei, EA Early apoptosis, LA Late apoptosis, AB Apoptotic bodies