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Table 1 HPLC conditions maintained for analysis of study drugs

From: Effect of various anticoagulants on the bioanalysis of drugs in rat blood: implication for pharmacokinetic studies of anticancer drugs

  Drugs
  Erlotinib Voriconazole Imatinib 6-MP Meropenem Pemetrexed
Mobile phase A:B = 40:60 A = acetonitrile B = ammonium acetate buffer (20 mM) pH 3.0 A:B = 35:65 A = acetonitrile B = ammonium dihydrogen phosphate buffer pH 7.0 A:B = 26.8:73.2 A = acetonitrile B = potassium dihydrogen phosphate buffer pH 3.0 A:B = 4:96 A = acetonitrile:methanol (50:50) B = triethylamine buffer pH 3.5 A:B = 5:95 A = acetonitrile:methanol (50:50) B = potassium dihydrogen phosphate buffer pH 6.2 A:B = 5:95 A = 1:1 methanol: ACN B = ammonium acetate buffer pH 6.2
Column C18 4.6 × 100 mm, 5 μ C18 4.6 × 100 mm, 5 μ C18 4.6 × 100 mm, 5 μ C18 4.6 × 100 mm, 5 μ C18 4.6 × 100 mm, 5 μ C18 4.6 × 100 mm, 5 μ
Column temperature (°C) 40 47 43 25 40 40
Flow rate (mL/min) 1 1 0.8 0.7 1 1
Retention time (min) 4.2 + 0.5 5.7 + 0.5 3.4 + 0.5 3.7 + 0.5 6.67 + 0.5 6.7 + 0.5
UV detection wavelength (nm) 247 255 265 324 297 255