Table 1 HPLC conditions maintained for analysis of study drugs
Drugs | ||||||
|---|---|---|---|---|---|---|
Erlotinib | Voriconazole | Imatinib | 6-MP | Meropenem | Pemetrexed | |
Mobile phase | A:B = 40:60 A = acetonitrile B = ammonium acetate buffer (20 mM) pH 3.0 | A:B = 35:65 A = acetonitrile B = ammonium dihydrogen phosphate buffer pH 7.0 | A:B = 26.8:73.2 A = acetonitrile B = potassium dihydrogen phosphate buffer pH 3.0 | A:B = 4:96 A = acetonitrile:methanol (50:50) B = triethylamine buffer pH 3.5 | A:B = 5:95 A = acetonitrile:methanol (50:50) B = potassium dihydrogen phosphate buffer pH 6.2 | A:B = 5:95 A = 1:1 methanol: ACN B = ammonium acetate buffer pH 6.2 |
Column | C18 4.6 × 100 mm, 5 μ | C18 4.6 × 100 mm, 5 μ | C18 4.6 × 100 mm, 5 μ | C18 4.6 × 100 mm, 5 μ | C18 4.6 × 100 mm, 5 μ | C18 4.6 × 100 mm, 5 μ |
Column temperature (°C) | 40 | 47 | 43 | 25 | 40 | 40 |
Flow rate (mL/min) | 1 | 1 | 0.8 | 0.7 | 1 | 1 |
Retention time (min) | 4.2 + 0.5 | 5.7 + 0.5 | 3.4 + 0.5 | 3.7 + 0.5 | 6.67 + 0.5 | 6.7 + 0.5 |
UV detection wavelength (nm) | 247 | 255 | 265 | 324 | 297 | 255 |