From: Molecular diagnostic markers of Tachysurus fulvidraco and Leiocassis longirostris and their hybrids
Locus | Primer sequences (5′–3′) | PCR products sizes (bp) | |||
---|---|---|---|---|---|
T. fulvidraco | F TL | L. Longirostris | F LT | ||
COI | F: CTACAATCCACCGCCTAA R: TAGAAGAAAGTGACAGAGCG | 1515 | 1515 | 1515 | 1515 |
ITS | F: GTAGGTGAACCTGCGGAAGGATCA R: GAGTTTACCACCCGCTTTGGGCTGCATT | 1106 | – | 1018 | – |
ITSP | F: CGTAACAAGGTTTCCGTAGGTG R: ATCCACCGCTAAGAGTTGTCAG | 678 | 602 and 678 | 602 | 602 and 678 |