Table 1 Primers and templates used in generation of deletion and truncated mutants for luciferase activity test
Construct | Primer name | Primer type | Template | |
|---|---|---|---|---|
pGL3-(−422) | bMx2 p 422 + KpnI | 5′-AGAGGTACCCCGTGGAGGAGCTGTGGATGTC | Forward | Genomic DNA |
bMx2 p 20 + XhoI | 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG | Reverse | ||
pGL-(−154) | bMx2 p154 + KpnI | 5′-AGAGGTACCCGCTTCAGGTTTCATTTCTG | Forward | Genomic DNA |
bMx2 p 20 + XhoI | 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG | Reverse | ||
pGL-(−132) | bMx2 p132 KpnI | 5′-AGAGGTACCGCAGGCTAATGGTTTCGTTT | Forward | Genomic DNA |
bMx2 p 20 + XhoI | 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG | Reverse | ||
pGL-(−109) | bMx2 p109 + KpnI | 5′-AGAGGTACCGGGCAAGCCATTAGTTTCAT | Forward | Genomic DNA |
bMx2 p 20 + XhoI | 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG | Reverse | ||
pGL-(−76) | bMx2 p76 + KpnI | 5′-AGAGGTACCGGGAAAGCAAGCCACGAG | Forward | Genomic DNA |
bMx2 p 20 + XhoI | 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG | Reverse | ||
pGL-ΔISRE1 + 2 | bMx2 p84F | 5′-TTACTTCTGGGAAAGCAAGC | Forward | pGL-(−154) |
bMx2 p127 | 5′-GCCTGCCACAGAAATGAAAC | Reverse | ||
pGL-ΔISRE1 | bMx2 p84F | 5′-TTACTTCTGGGAAAGCAAGC | Forward | pGL-(−154) |
bMx2 p99 | 5′-ATGGCTTGCCCTAGAAACG | Reverse | ||
pGL-ΔISRE2 | bMx2 p109 | 5′-GGGCAAGCCATTAGTTTCAT | Forward | pGL-(−154) |
bMx2 p127 | 5′-GCCTGCCACAGAAATGAAAC | Reverse | ||