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Table 1 Primers and templates used in generation of deletion and truncated mutants for luciferase activity test

From: Molecular cloning, polymorphism, and functional activity of the bovine and water buffalo Mx2 gene promoter region

Construct Primer name Primer type Template
pGL3-(−422) bMx2 p 422 + KpnI 5′-AGAGGTACCCCGTGGAGGAGCTGTGGATGTC Forward Genomic DNA
  bMx2 p 20 + XhoI 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG Reverse  
pGL-(−154) bMx2 p154 + KpnI 5′-AGAGGTACCCGCTTCAGGTTTCATTTCTG Forward Genomic DNA
  bMx2 p 20 + XhoI 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG Reverse  
pGL-(−132) bMx2 p132 KpnI 5′-AGAGGTACCGCAGGCTAATGGTTTCGTTT Forward Genomic DNA
  bMx2 p 20 + XhoI 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG Reverse  
pGL-(−109) bMx2 p109 + KpnI 5′-AGAGGTACCGGGCAAGCCATTAGTTTCAT Forward Genomic DNA
  bMx2 p 20 + XhoI 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG Reverse  
pGL-(−76) bMx2 p76 + KpnI 5′-AGAGGTACCGGGAAAGCAAGCCACGAG Forward Genomic DNA
  bMx2 p 20 + XhoI 5′-AGACTCGAGCCTGTGCACTTGTAGGAGGG Reverse  
pGL-ΔISRE1 + 2 bMx2 p84F 5′-TTACTTCTGGGAAAGCAAGC Forward pGL-(−154)
  bMx2 p127 5′-GCCTGCCACAGAAATGAAAC Reverse  
pGL-ΔISRE1 bMx2 p84F 5′-TTACTTCTGGGAAAGCAAGC Forward pGL-(−154)
  bMx2 p99 5′-ATGGCTTGCCCTAGAAACG Reverse  
pGL-ΔISRE2 bMx2 p109 5′-GGGCAAGCCATTAGTTTCAT Forward pGL-(−154)
  bMx2 p127 5′-GCCTGCCACAGAAATGAAAC Reverse