Table 1 Detection methods and success rate (efficiency)
From: A simple and efficient total genomic DNA extraction method for individual zooplankton
TGDNA extraction methods | Detection methods | Success rate (efficiency) (%) | ||
|---|---|---|---|---|
AGE | Spectrophotometer | PCR | \( \frac{success}{50\;trails} \times 100\% \) | |
2× CTAB | − | − | − | 0 |
Phenol chloroform | − | − | − | 0 |
KAPA express extract | − | − | + | 46 |
Direct boiling | − | − | − | 0 |
Incubation in lysis buffer and proteinase K | − | − | + | 18 |
Incubation in PCR buffer | − | − | + | 82 |