Table 1 Primers for q-PCR and RT-PCR
From: Identification of differentially methylated regions (DMRs) of neuronatin in mice
Gene | Primer sequence (5′ → 3′) | Tann (°C) | Length (bp) |
|---|---|---|---|
NNAT–RT | F:GCTCATCATCGGCTGGTACA | 60 | 343 |
R:CTTGGCAAGTGCTCCTCTGA | 262 | ||
β-actin-RT | F:ATATCGCTGCGCTGGTCGTC | 60 | 517 |
R:AGGATGGCGTGAGGGAGAGC | |||
NNAT-q | F:GTCCCCTGTGTTCCCTCGTC | 60 | 81 |
R:TGTCGGTGCTGCTTTTCTGG | |||
Actin-q | F:GGCACCACACYTTCTACAATG | 60 | 133 |
R:GGGGTGTTGAAGGTCTCAAAC |