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Table 1 Callus and shoot formation (%) from base segments of rye (Secale cereale) on MS and N6 medium supplemented with carbohydrate and plant growth regulators

From: Efficient regeneration system from rye leaf base segments

Basal medium Carbohydrate source Combination of plant growth regulatorsa Callus formation (%) Shoot formation (%)
MS Maltose A 12.50 2.50
B 15.00 0.00
C 27.50 8.75
D 11.25 2.50
Average 16.56 3.44
Sucrose A 16.25 3.75
B 7.50 7.50
C 15.00 18.75
D 21.25 10.00
Average 15.00 10.00
Average A 14.38 3.13
B 11.25 3.75
C 21.25 13.75
D 16.25 6.25
Average 15.78 6.72
N6 Maltose A 1.25 3.75
B 1.25 1.25
C 12.50 3.75
D 12.50 2.50
Average 6.88 2.81
Sucrose A 2.50 0.00
B 1.25 0.00
C 1.25 1.25
D 7.50 6.25
Average 3.13 1.88
Average A 1.88 1.88
B 1.25 0.63
C 6.88 2.50
D 10.00 4.38
Average 5.00 2.34
The average of medium Maltose A 6.88 3.13
B 8.13 0.63
C 20.00 6.25
D 11.88 2.50
Average 11.72 3.13
Sucrose A 9.38 1.88
B 4.38 3.75
C 8.13 10.00
D 14.38 8.13
Average 9.06 5.94
Average A 8.13 2.50
B 6.25 2.19
C 14.06 8.13
D 13.13 5.31
Average 10.39 4.53
F value Basal medium (M) 42.383** 13.364**
Carbohydrate source (C) 2.573ns 5.523*
Combination of plant growth regulators (R) 5.255** 5.386**
M × C 0.436ns 9.818**
M × R 1.125ns 3.773*
C × R 4.234* 1.477ns
M × C × R 2.050ns 0.682ns
LSD(0.05) Basal medium (M) 3.33 2.41
Carbohydrate source (C) 2.41
Combination of plant growth regulators (R) 4.71 3.40
M × C 3.40
M × R 4.81
C × R 6.66
M × C × R
  1. aA: 1 mg/L 2,4-D + 1 mg/L TDZ; B: 1 mg/L 2,4-D + 2 mg/L TDZ; C: 2 mg/L 2,4-D + 1 mg/L TDZ; D: 2 mg/L 2,4-D + 2 mg/L TDZ
  2. * Significant at the 0.05 probability level
  3. ** Significant at the 0.01 probability level
  4. ns Non significant