Table 1 Callus and shoot formation (%) from base segments of rye (Secale cereale) on MS and N6 medium supplemented with carbohydrate and plant growth regulators
From: Efficient regeneration system from rye leaf base segments
Basal medium | Carbohydrate source | Combination of plant growth regulatorsa | Callus formation (%) | Shoot formation (%) |
|---|---|---|---|---|
MS | Maltose | A | 12.50 | 2.50 |
B | 15.00 | 0.00 | ||
C | 27.50 | 8.75 | ||
D | 11.25 | 2.50 | ||
Average | 16.56 | 3.44 | ||
Sucrose | A | 16.25 | 3.75 | |
B | 7.50 | 7.50 | ||
C | 15.00 | 18.75 | ||
D | 21.25 | 10.00 | ||
Average | 15.00 | 10.00 | ||
Average | A | 14.38 | 3.13 | |
B | 11.25 | 3.75 | ||
C | 21.25 | 13.75 | ||
D | 16.25 | 6.25 | ||
Average | 15.78 | 6.72 | ||
N6 | Maltose | A | 1.25 | 3.75 |
B | 1.25 | 1.25 | ||
C | 12.50 | 3.75 | ||
D | 12.50 | 2.50 | ||
Average | 6.88 | 2.81 | ||
Sucrose | A | 2.50 | 0.00 | |
B | 1.25 | 0.00 | ||
C | 1.25 | 1.25 | ||
D | 7.50 | 6.25 | ||
Average | 3.13 | 1.88 | ||
Average | A | 1.88 | 1.88 | |
B | 1.25 | 0.63 | ||
C | 6.88 | 2.50 | ||
D | 10.00 | 4.38 | ||
Average | 5.00 | 2.34 | ||
The average of medium | Maltose | A | 6.88 | 3.13 |
B | 8.13 | 0.63 | ||
C | 20.00 | 6.25 | ||
D | 11.88 | 2.50 | ||
Average | 11.72 | 3.13 | ||
Sucrose | A | 9.38 | 1.88 | |
B | 4.38 | 3.75 | ||
C | 8.13 | 10.00 | ||
D | 14.38 | 8.13 | ||
Average | 9.06 | 5.94 | ||
Average | A | 8.13 | 2.50 | |
B | 6.25 | 2.19 | ||
C | 14.06 | 8.13 | ||
D | 13.13 | 5.31 | ||
Average | 10.39 | 4.53 | ||
F value | Basal medium (M) | 42.383** | 13.364** | |
Carbohydrate source (C) | 2.573ns | 5.523* | ||
Combination of plant growth regulators (R) | 5.255** | 5.386** | ||
M × C | 0.436ns | 9.818** | ||
M × R | 1.125ns | 3.773* | ||
C × R | 4.234* | 1.477ns | ||
M × C × R | 2.050ns | 0.682ns | ||
LSD(0.05) | Basal medium (M) | 3.33 | 2.41 | |
Carbohydrate source (C) | – | 2.41 | ||
Combination of plant growth regulators (R) | 4.71 | 3.40 | ||
M × C | – | 3.40 | ||
M × R | – | 4.81 | ||
C × R | 6.66 | – | ||
M × C × R | – | – | ||