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Fig. 3 | SpringerPlus

Fig. 3

From: Purification and antigenic detection of O-specific polysaccharides of Salmonella enterica serovar Paratyphi A isolate from Pakistan: an emerging threat

Fig. 3

a The antigen antibody interaction in vitro, LPS of S. Paratyphi A by immunodiffusion assay. Well 1 LPS (250 μg/ml) in normal saline, well 2 and 3 shows Mice serum (1/8 time diluted polyclonal antibodies). The arrow shows precipitin line between S. Paratyphi A LPS and hyper immune mice serum raised against S. Paratyphi A, indicating LPS are antigenically active. b Antigenic evaluation of OSP against hyper immune mice sera by immunodiffusion assay Well 1 OSP (250 μg/ml) in normal saline, well 2 Negative control (normal saline), Well 3 Mice serum (1/8 time diluted polyclonal antibodies). The arrow shows precipitin line between S. Paratyphi A OSP and hyper immune mice serum raised against S. Paratyphi A, indicating antigenicity well maintained during purification procedure

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