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Table 2 Nucleotide sequence variants of the 346-bp E-gene region isolated from human plasma and mosquito samples compared with their corresponding master sequences (hu2 and mo1)

From: Genomic studies of envelope gene sequences from mosquito and human samples from Bangkok, Thailand

Sequence variant No. of clones (% of total) Clones No. of nucleotide substitutionsa Total no. of substitutionsb dN/dSc,d
hu1 1 (1.80) 36-2 1 1 0.16
hu2 16 (29.1) 36-3, 4, 6, 8, 9, 10, 11, 12
47-2, 3, 5, 6, 7, 8, 9, 10
0 0 1.00
hu3 1 (1.8) 36-14 1 1 0.16
hu4 1 (1.8) 47-4 1 1 1.00
hu5 1 (1.8) 47-1 1 1 1.00
hu6 10 (18) 37-1, 2, 3, 4, 5, 6, 8, 10, 16, 18 12 120  
hu7 9 (16.4) 41-2, 4, 9, 18, 23, 24, 26, 27, 28 10 90 1.00
hu8 1 (1.8) 45-1 10 10 1.00
hu9 4 (7.3) 45-5, 6, 14, 25 9 36 1.00
hu10 1 (1.8) 45-15 10 10 1.00
hu11 8 (14.5) 50-2, 3, 4, 6, 7, 8, 9, 10 11 88 1.00
hu12 1 (1.8) 50-1 12 12 1.00
hu13 1 (1.8) 50-5 12 12 1.00
Total 55    382  
m1 11 (39.3) 56-4, 7, 15
275-24, 25, 27, 30, 32, 33, 35, 36
0 0 1.00
m2 1 (3.5) 56-20 1 1 0.16
m3 1 (3.5) 275-26 1 1 0.16
m4 1 (3.5) 275-31 2 2 0.08
m5 2 (7.1) 275-37, 38 9 18 1.00
m6 1 (3.5) 275-39 1 1 0.16
m7 1 (3.5) 275-40 1 1 0.16
m8 8 (28.6) 56-2, 5, 6, 10, 12, 13, 14, 17 12 96 1.00
m9 1 (7.1) 56-3 14 14 1.00
m10 1 (3.5) 56-8 13 13 1.00
Total 28    147  
  1. aNumber of substitutions relative to master sequences hu2 and mo1
  2. bTotal number of substitutions in each variant multiplied by the number of clones of that variant
  3. cRatio of non-synonymous to synonymous substitutions per site, calculated using the codon-based test of positive selection in MEGA
  4. d p value = 0.05