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Table 2 Nucleotide sequence variants of the 346-bp E-gene region isolated from human plasma and mosquito samples compared with their corresponding master sequences (hu2 and mo1)

From: Genomic studies of envelope gene sequences from mosquito and human samples from Bangkok, Thailand

Sequence variant

No. of clones (% of total)

Clones

No. of nucleotide substitutionsa

Total no. of substitutionsb

dN/dSc,d

hu1

1 (1.80)

36-2

1

1

0.16

hu2

16 (29.1)

36-3, 4, 6, 8, 9, 10, 11, 12

47-2, 3, 5, 6, 7, 8, 9, 10

0

0

1.00

hu3

1 (1.8)

36-14

1

1

0.16

hu4

1 (1.8)

47-4

1

1

1.00

hu5

1 (1.8)

47-1

1

1

1.00

hu6

10 (18)

37-1, 2, 3, 4, 5, 6, 8, 10, 16, 18

12

120

 

hu7

9 (16.4)

41-2, 4, 9, 18, 23, 24, 26, 27, 28

10

90

1.00

hu8

1 (1.8)

45-1

10

10

1.00

hu9

4 (7.3)

45-5, 6, 14, 25

9

36

1.00

hu10

1 (1.8)

45-15

10

10

1.00

hu11

8 (14.5)

50-2, 3, 4, 6, 7, 8, 9, 10

11

88

1.00

hu12

1 (1.8)

50-1

12

12

1.00

hu13

1 (1.8)

50-5

12

12

1.00

Total

55

  

382

 

m1

11 (39.3)

56-4, 7, 15

275-24, 25, 27, 30, 32, 33, 35, 36

0

0

1.00

m2

1 (3.5)

56-20

1

1

0.16

m3

1 (3.5)

275-26

1

1

0.16

m4

1 (3.5)

275-31

2

2

0.08

m5

2 (7.1)

275-37, 38

9

18

1.00

m6

1 (3.5)

275-39

1

1

0.16

m7

1 (3.5)

275-40

1

1

0.16

m8

8 (28.6)

56-2, 5, 6, 10, 12, 13, 14, 17

12

96

1.00

m9

1 (7.1)

56-3

14

14

1.00

m10

1 (3.5)

56-8

13

13

1.00

Total

28

  

147

 
  1. aNumber of substitutions relative to master sequences hu2 and mo1
  2. bTotal number of substitutions in each variant multiplied by the number of clones of that variant
  3. cRatio of non-synonymous to synonymous substitutions per site, calculated using the codon-based test of positive selection in MEGA
  4. d p value = 0.05