Genomic studies of envelope gene sequences from mosquito and human samples from Bangkok, Thailand

SpringerPlus

Table 1 Nucleotide and amino acid sequence diversity of the E-gene region of DENV-2 from infected patients and mosquitoes

Sample	Disease	No. of clones					Amino acid sequences
			No. of substitutions^a		Mean diversity (%)^c	Mean distance (10⁻²)^c	No. of substitutions^a	Mean diversity (%)^b	Mean distance (10⁻²)^c
			Non-silent	Silent	Mean diversity (%)^c	Mean distance (10⁻²)^c	No. of substitutions^a	Mean diversity (%)^b	Mean distance (10⁻²)^c
ID36	DF	10	2	0	2/3460 (0.058)	0.12	2	2/1150 (0.17)	0.35
ID37	DHF	10	10	110	120/3460 (3.47)	0.00	10	10/1150 (0.87)	0
ID41	DHF	9	9	81	90/3114 (2.89)	0.00	9	9/1035 (0.87)	0
ID45	DF	6	1	55	56/2076 (2.69)	0.19	1	1/690 (0.14)	0.29
ID47	DHF	10	0	2	2/3460 (0.058)	0.12	0	0/1150 (0.00)	0
ID50	DHF	10	21	91	112/3460 (3.24)	0.12	21	21/1150 (1.83)	0.17
Humans		55	42	340	382/19030 (2.01)		42	42/6325 (0.66)
Mos56		14	12	112	124/4844 (2.56)	1.69	12	12/1610 (0.75)	0.63
Mos275		14	5	18	23/4844 (0.47)	0.89	5	5/1610 (0.31)	0.61
Mosquitoes		28	17	130	147/9660 (1.52)		17	17/3220 (0.53)

DF dengue fever, DHF dengue hemorrhagic fever
^aNumber of substitutions relative to master sequences hu2 and m1
^bNumber of nucleotide (or amino acid) substitutions divided by the total number of sequenced nucleotides (or amino acids) multiplied by 100
^cCalculated by pairwise comparison of nucleotide or amino acid sequences between clones within each sample using within- and between-group mean distances in MEGA