Molecular characterization and phylogenetic analysis of dengue virus type 1 in Guangdong in 2014

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Table 5 Nucleotide sequence of primers used in the PCR assay

Name	Forward primer sequence (5′ → 3′)	Position	Name	Reverse primer sequence (5′ → 3′)	Position
1F	GTTAGTCTACGTGGACCGAC	6–25	1R	CCAACACCACATCTACCCAA	992–1011
2F	TAGCACATGCCATAGGAACATC	856–887	2R	CCTTCGTATTTAACCTGCACTAG	1896–1918
3F	TCACAAGAAGGAGCAATGCACA	1698–1719	3R	AAGAAGAACTTCTCTGGATGTTA	3763–3785
4F	TGGAGCCAACGCTTCCGACA	3651–3675	4R	TCCCAGGAGACCTCAGCCGC	4296–4315
5F	ATATCTGGAAGCTCAGCCGA	4260–4279	5R	CCCTGGTTCAACAGCAATCA	4810–4829
6F	CTGTACTCATGTATCAAGGGAAGA	4687–4710	6R	CTTGGATAACTGCGTTGCTCTG	5502–5523
7F	AGGGAATGCCAATAAGGTAC	5230–5249	7R	GTCCTGCTAAGATGACACGC	5840–5859
8F	CAGAGCAACGCAGTTATCCA	5502–5521	8R	CAATTTAGCGGTTCCTCTCG	7741–7760
9F	GCAAGTCAGAATTCAACACC	7636–7655	9R	CATATGATCCATGATAGGCC	8480–8499
10F	CGATTCACAATGGCTCACAGGA	8295–8316	10R	ATGGCACCACTATTTCCCTCCC	9732–9753
11F	CTAACCTACCAAAATAAAGT	9383–9301	11R	TTAGTCTTCTCACTTGGTTT	10,172–10,191
12F	CCATATTTAGGGAAAAGGGA	10,080–10,099	12R	CGCCTGGAATGATGCTGTAG	10,672–10,691
E 1F	TAGCACATGCCATAGGAA	856–873	E 1R	CTGGGTCTCAGCCACTTC	1866–1883
E 2F	ATGCAAAGAAGCAGGAAG	1666–1683	E 2R	AATTTGTATTGCTCTGTCCA	2502–2521
E 3F	CATAGGAACATCCATCACC	866–884	E 3R	TATTGCTCTGTCCAAGTGTG	2496–2515
E 4F	GAACATCCATCACCCAGAAA	871–890	E 4R	GCTCTGTCCAAGTGTGAACTT	2491–2511