Table 1 Primer sequences used for studied genes mRNA expression assay
Primer | 5′-3′ sequence | Fragment size in base pairs |
|---|---|---|
UCP1-ex3/4_F | GTCTTTGGAAAGGGACTACT | 125 |
UCP1-ex5/6_R | GGGGACGTCATCTGCTAATA | |
UCP1-P | FAM-AGTGTCATCATCAATTGTACAGAGCT-BHQ | |
CYP19A1 ex3/4-F | TTATCAGCAAGTCCTCAAGTAT | 179 |
CYP19A1 ex4/5-R | GGGGCCTGACAGAGCTTTC | |
CYP19A1 ex4-P | FAM-AGGCATCATATTTAACAACAATCCAGA-BHQ | |
Omentin-F | AACAGCTCCCTGCTGAGGTA | 199 |
Omentin-R | GCTGGCCATAGGGTGAGTAA | |
Omentin-P | FAM-CATCTACCAGAAATATCCAGTGAAAT-BHQ | |
PTEN ex 5/6-F | GAGACAAAAAGGGAGTAACTAT | 155 |
PTEN ex 6/7-R | ATTGCAAGTTCCGCCACTGA | |
PTEN ex6-P | FAM-TATAGCTACCTGTTAAAGAATCATCTG-BHQ | |
SDHA-F | CCACTCGCTATTGCACACC | 102 |
SDHA-R | CACTCCCCGTTCTCCATCA | |
SDHA-P | JOE-ACGGTCTCTGCGATATGATACCA-BHQ |