Fig. 5

RT-PCR analysis of hypothalamic OB-Rb mRNA expression. The OD values of the OB-Rb mRNA were 29.15 ± 7.96, 14.89 ± 4.98, 5.96 ± 2.12 and 5.61 ± 1.13 in the normal control (NC), DIO4 W (fed a high-fat diet for 4 weeks), DIO8 W group (fed a high-fat diet for 8 weeks), and T2DM groups (fed a high-fat diet for 8 weeks + STZ intraperitoneal injection), and also showed a downward trend (P < 0.01). *P < 0.05 vs. NC group