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Table 1 Effect of plant growth regulators (PGRs) on shoot differentiation of D. hamiltonii

From: Callus induction and regeneration via shoot tips of Dendrocalamus hamiltonii

Orthogonal combination

PGRs (mg/l)

Shoot differentiation rate (%)

Shoot growth pattern

BA

KT

NAA

1

1

0.1

0.1

21.67 ± 1.67c

Yellow–green; few buds

2

1

0.3

0.3

51.65 ± 1.65a

Yellow–green; fascicled buds

3

1

1

1

18.39 ± 1.62d

Yellow–green; light browning

4

2

0.1

0.3

28.35 ± 1.65b

Yellow–green; few buds

5

2

0.3

1

23.32 ± 0.02bc

Yellow–green; few buds

6

2

1

0.1

21.67 ± 1.67c

Yellow–green; few buds

7

4

0.1

1

18.39 ± 1.62d

Yellow–green; few buds

8

4

0.3

0.1

21.67 ± 1.67c

Yellow–green; few buds

9

4

1

0.3

20.00 ± 0.02c,d

Yellow–green; albino

X1

30.57

22.80

21.67

  

X2

24.45

32.21

33.33

  

X3

20.02

20.02

20.03

  

R

10.55

12.19

13.30

  
  1. Values represent the mean ± standard error. Values within the same column followed by the same letter are not significantly different according to the least significant difference at P < 0.05 (Duncan 1955)
  2. X mean value of the test corresponding to a factor in the same level. R = Xmax − Xmin
  3. Each treatment consisted of three replications of 20 explants each. All data were collected 4 weeks after inoculated into the medium supplemented with PGRs
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