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Table 1 Effect of plant growth regulators (PGRs) on shoot differentiation of D. hamiltonii

From: Callus induction and regeneration via shoot tips of Dendrocalamus hamiltonii

Orthogonal combination PGRs (mg/l) Shoot differentiation rate (%) Shoot growth pattern
BA KT NAA
1 1 0.1 0.1 21.67 ± 1.67c Yellow–green; few buds
2 1 0.3 0.3 51.65 ± 1.65a Yellow–green; fascicled buds
3 1 1 1 18.39 ± 1.62d Yellow–green; light browning
4 2 0.1 0.3 28.35 ± 1.65b Yellow–green; few buds
5 2 0.3 1 23.32 ± 0.02bc Yellow–green; few buds
6 2 1 0.1 21.67 ± 1.67c Yellow–green; few buds
7 4 0.1 1 18.39 ± 1.62d Yellow–green; few buds
8 4 0.3 0.1 21.67 ± 1.67c Yellow–green; few buds
9 4 1 0.3 20.00 ± 0.02c,d Yellow–green; albino
X1 30.57 22.80 21.67   
X2 24.45 32.21 33.33   
X3 20.02 20.02 20.03   
R 10.55 12.19 13.30   
  1. Values represent the mean ± standard error. Values within the same column followed by the same letter are not significantly different according to the least significant difference at P < 0.05 (Duncan 1955)
  2. X mean value of the test corresponding to a factor in the same level. R = Xmax − Xmin
  3. Each treatment consisted of three replications of 20 explants each. All data were collected 4 weeks after inoculated into the medium supplemented with PGRs