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Fig. 2 | SpringerPlus

Fig. 2

From: Efficacy of indirect ELISA for serodiagnosis of melioidosis using immunodominant antigens from non-pathogenic Burkholderia thailandensis

Fig. 2

The expressed and purified OmpABT was confirmed by 12 % SDS-PAGE (a) and western blotting (b). Lanes 1 and 2, total cell lysate of E. coli harboring pET24a-OmpABT of uninduced and induced clone with IPTG, respectively, Lane 3, soluble fraction, Lane 4, inclusion bodies fraction, Lane 5, concentrated eluted protein and Lane M, protein molecular weight marker. Relative molecular mass (kDa) of standard proteins is shown on the left-hand side. The arrowhead indicates the location of targeted protein

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