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Table 2 Effect of inhibitors, detergents, chelating agents, oxidizing agents and reducing agents on the activity of purified β-mannanase

From: Kinetic study of a β-mannanase from the Bacillus licheniformis HDYM-04 and its decolorization ability of twenty-two structurally different dyes

Reagent

Relative activity (%)

Control

100a

Inhibitors

 

 Citric acid

87.53 ± 2.34b

 Oxalic acid

68.76 ± 1.98c

 Sodium thioglycolate

45.34 ± 2.67d

 Hydrogen

37.29 ± 1.34de

 Phenylmethyl sulfonyl fluoride

26.98 ± 2.44e

Detergents

 

 Polyethylene glycol

79.62 ± 2.47b

 Cetyl trimethyl ammonium bromide

14.15 ± 1.21f

Chelating agents

 

 Ethylene diamine teraacetic acid

82.43 ± 1.23b

 Sodium citrate

78.87 ± 3.23b

 Sodium azide

65.98 ± 1.34c

Oxidizing agents

 

 Potassium iodide

83.23 ± 1.23b

 Ammonium persulfate

66.37 ± 1.19c

 Hydrogen peroxide

45.82 ± 2.12d

Reducing agents

 

 Ascorbic acid

38.81 ± 1.13de

 Dithiothreitol

27.63 ± 2.41e

  1. The 100 % activity represented the control enzyme activity without any agents. Different letters represent significant differences (p < 0.05) relative to the control