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Table 2 Effect of inhibitors, detergents, chelating agents, oxidizing agents and reducing agents on the activity of purified β-mannanase

From: Kinetic study of a β-mannanase from the Bacillus licheniformis HDYM-04 and its decolorization ability of twenty-two structurally different dyes

Reagent Relative activity (%)
Control 100a
Inhibitors  
 Citric acid 87.53 ± 2.34b
 Oxalic acid 68.76 ± 1.98c
 Sodium thioglycolate 45.34 ± 2.67d
 Hydrogen 37.29 ± 1.34de
 Phenylmethyl sulfonyl fluoride 26.98 ± 2.44e
Detergents  
 Polyethylene glycol 79.62 ± 2.47b
 Cetyl trimethyl ammonium bromide 14.15 ± 1.21f
Chelating agents  
 Ethylene diamine teraacetic acid 82.43 ± 1.23b
 Sodium citrate 78.87 ± 3.23b
 Sodium azide 65.98 ± 1.34c
Oxidizing agents  
 Potassium iodide 83.23 ± 1.23b
 Ammonium persulfate 66.37 ± 1.19c
 Hydrogen peroxide 45.82 ± 2.12d
Reducing agents  
 Ascorbic acid 38.81 ± 1.13de
 Dithiothreitol 27.63 ± 2.41e
  1. The 100 % activity represented the control enzyme activity without any agents. Different letters represent significant differences (p < 0.05) relative to the control