Table 2 Effect of metal ions and inhibitors on the proteolyticactivity of hatching enzyme
From: Characterization of a novel hatching enzyme purified from starfish Asterina pectinifera
Inhibitors or metal ions | Concentration (mM) | Relative activity (%) |
|---|---|---|
EDTA | 5 | 38.15 ± 9.86 |
EGTA | 5 | 42.31 ± 8.41 |
Cu2+ | 10 | 75.38 ± 7.01 |
Mg2+ | 10 | 71.22 ± 4.65 |
Zn2+ | 10 | 167.28 ± 12.69 |
Ca2+ | 10 | 86.47 ± 2.50 |
Leupeptin | 0.1 | 56.29 ± 2.57 |
PMSF | 0.1 | 56.20 ± 4.15 |
TLCK | 0.1 | 56.72 ± 2.34 |
TPCK | 0.1 | 40.47 ± 8.40 |