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Table 5 The genotyping results of 66 clinical throat swab samples

From: Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray

No. H1a (Ct) N1b (Ct) H3c (Ct) H7d (Ct) Microarray results No. H1 (Ct) N1 (Ct) H3 (Ct) H7 (Ct) Microarray results
1 27.79 29.59 PH1N1 + A 34 32.71 33.01 A
2 39.20 A 35 21.11 21.14 PH1N1 + A
3 34.26 A 36 21.08 22.59 PH1N1 + A
4 26.04 29.06 PH1N1 + A 37 29.17 31.4 PH1N1 + A
5 37.23 A 38 27.30 H3N2 + A
6 32.57 H3N2 + A 39
7 23.23 25.41 PH1N1 + A 40 30.06 31.83 PH1N1 + A
8 26.74 28.78 PH1N1 + A 41 25.34 21.22 PH1N1 + A
9 31.15 A 42 32.88 34.43 A
10 21.06 22.65 PH1N1 + A 43 20.37 21.41 PH1N1 + A
11 17.43 19.43 PH1N1 + A 44 26.09 26.3 PH1N1 + A
12 31.91 H3N2 + A 45 31.53 A
13 30.29 A 46 32.59 33.09
14 A 47 26.88 27.87 PH1N1 + A
15 25.79 26.51 PH1N1 + A 48 38.43 A
16 35.71 36.76 49
17 30.20 33.15 PH1N1 + A 50 31.75 31.37 PH1N1 + A
18 28.48 28.42 PH1N1 + A 51 35.09 A
19 27.42 26.41 33.61 PH1N1 + A 52 30.63 31.68 A
20 28.91 31.06 30.64 PH1N1 + H3N2 + A 53 35.10 35.15
21 27.97 29.61 PH1N1 + A 54 36.08 A
22 A 55 38.69 A
23 56 A
24 27.43 27.58 PH1N1 + A 57
25 A 58
26 26.63 28.38 PH1N1 + A 59 33.94 A
27 28.53 30 PH1N1 + A 60
28 35.71 35.16 61 27.38 27.57 PH1N1 + A
29 38.22 A 62 32.67 35.06 A
30 23.68 26.25 PH1N1 + A 63 24.28 21.9 PH1N1 + A
31 33.90 35.31 A 64 33.17 32.83
32 33.76 34.12 A 65 32.53 32.64 A
33 33.82 35.42 A 66 30.55 31.76 A
  1. aThe real-time PCR reference reagent of H1 was a 2009 Influenza A Virus (H1N1) Nucleic Acid Detection Kit (PCR-Fluorescent probe) (Shenzhen Puruikang Biotech Co., Ltd.)
  2. bThe real-time PCR reference reagent of N1 was 2009 Influenza A Virus (H1N1) Nucleic Acid Detection Kit (PCR-fluorescent probe) (DAAN Gene Co., Ltd.)
  3. cThe real time PCR reference reagent of H3 was Seasonal Influenza A Virus (H3) Nucleic Acid Detection Kit (PCR-Fluorescent probe) (DAAN Gene Co., Ltd.)
  4. d The real time PCR reference reagent of H7 was Diagnosis Kit for H7N9 Avian Influenza Virus RNA (PCR-fluorescent probe) (Shenzhen Puruikang Biotech Co., Ltd.)
  5. Results indicated that 25 of the samples were 2009 influenza A (H1N1), 3 were influenza A (H3N2), 26 were influenza A, 1 contained both 2009 influenza A (H1N1) and influenza A (H3N2), and 11 were negative. The results showed that microarray always failed to determine the subtypes of samples when the CT value of real-time RT-PCR exceeded 31. However, general detection of influenza A virus is possible even when the CT values were as high as 39. Consequently, general detection of influenza A virus was more sensitive than genotyping and detection of influenza A virus subtypes