Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray

Zhang, Yingjie; Liu, Qiqi; Wang, Dou; Chen, Suhong; Wang, Xiaobo; Wang, Shengqi

doi:10.1186/s40064-016-3482-9

SpringerPlus

Table 5 The genotyping results of 66 clinical throat swab samples

From: Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray

No.	H1^a (Ct)	N1^b (Ct)	H3^c (Ct)	H7^d (Ct)	Microarray results	No.	H1 (Ct)	N1 (Ct)	H3 (Ct)	H7 (Ct)	Microarray results
1	27.79	29.59	–	–	PH1N1 + A	34	32.71	33.01	–	–	A
2	–	–	39.20	–	A	35	21.11	21.14	–	–	PH1N1 + A
3	–	–	34.26	–	A	36	21.08	22.59	–	–	PH1N1 + A
4	26.04	29.06	–	–	PH1N1 + A	37	29.17	31.4	–	–	PH1N1 + A
5	–	–	37.23	–	A	38	–	–	27.30	–	H3N2 + A
6	–	–	32.57	–	H3N2 + A	39	–	–	–	–	–
7	23.23	25.41	–	–	PH1N1 + A	40	30.06	31.83	–	–	PH1N1 + A
8	26.74	28.78	–	–	PH1N1 + A	41	25.34	21.22	–	–	PH1N1 + A
9	–	–	31.15	–	A	42	32.88	34.43	–	–	A
10	21.06	22.65	–	–	PH1N1 + A	43	20.37	21.41	–	–	PH1N1 + A
11	17.43	19.43	–	–	PH1N1 + A	44	26.09	26.3	–	–	PH1N1 + A
12	–	–	31.91	–	H3N2 + A	45	–	–	31.53	–	A
13	–	30.29	–	–	A	46	32.59	33.09	–	–	–
14	–	–	–	–	A	47	26.88	27.87	–	–	PH1N1 + A
15	25.79	26.51	–	–	PH1N1 + A	48	–	–	38.43	–	A
16	35.71	36.76	–	–	–	49	–	–	–	–	–
17	30.20	33.15	–	–	PH1N1 + A	50	31.75	31.37	–	–	PH1N1 + A
18	28.48	28.42	–	–	PH1N1 + A	51	–	–	35.09	–	A
19	27.42	26.41	33.61	–	PH1N1 + A	52	30.63	31.68	–	–	A
20	28.91	31.06	30.64	–	PH1N1 + H3N2 + A	53	35.10	35.15	–	–	–
21	27.97	29.61	–	–	PH1N1 + A	54	–	–	36.08	–	A
22	–	–	–	–	A	55	–	–	38.69	–	A
23	–	–	–	–	–	56	–	–	–	–	A
24	27.43	27.58	–	–	PH1N1 + A	57	–	–	–	–	–
25	–	–	–	–	A	58	–	–	–	–	–
26	26.63	28.38	–	–	PH1N1 + A	59	–	–	33.94	–	A
27	28.53	30	–	–	PH1N1 + A	60	–	–	–	–	–
28	35.71	35.16	–	–	–	61	27.38	27.57	–	–	PH1N1 + A
29	–	–	38.22	–	A	62	32.67	35.06	–	–	A
30	23.68	26.25	–	–	PH1N1 + A	63	24.28	21.9	–	–	PH1N1 + A
31	33.90	35.31	–	–	A	64	33.17	32.83	–	–	–
32	33.76	34.12	–	–	A	65	32.53	32.64	–	–	A
33	33.82	35.42	–	–	A	66	30.55	31.76	–	–	A

^aThe real-time PCR reference reagent of H1 was a 2009 Influenza A Virus (H1N1) Nucleic Acid Detection Kit (PCR-Fluorescent probe) (Shenzhen Puruikang Biotech Co., Ltd.)
^bThe real-time PCR reference reagent of N1 was 2009 Influenza A Virus (H1N1) Nucleic Acid Detection Kit (PCR-fluorescent probe) (DAAN Gene Co., Ltd.)
^cThe real time PCR reference reagent of H3 was Seasonal Influenza A Virus (H3) Nucleic Acid Detection Kit (PCR-Fluorescent probe) (DAAN Gene Co., Ltd.)
^d The real time PCR reference reagent of H7 was Diagnosis Kit for H7N9 Avian Influenza Virus RNA (PCR-fluorescent probe) (Shenzhen Puruikang Biotech Co., Ltd.)
Results indicated that 25 of the samples were 2009 influenza A (H1N1), 3 were influenza A (H3N2), 26 were influenza A, 1 contained both 2009 influenza A (H1N1) and influenza A (H3N2), and 11 were negative. The results showed that microarray always failed to determine the subtypes of samples when the CT value of real-time RT-PCR exceeded 31. However, general detection of influenza A virus is possible even when the CT values were as high as 39. Consequently, general detection of influenza A virus was more sensitive than genotyping and detection of influenza A virus subtypes

Back to article page